NDB ID: PR0007

Features

RNA single strand

Compound name

POLYDENYLATE BINDING PROTEIN 1/RNA COMPLEX

Sequence in asymmetric unit

Chain A: A A A A A A A A A A A
Chain B: A A A A A A A A A A A
Chain C: A A A A A A A A A A A
Chain D: A A A A A A A A A A A
Chain E: A A A A A A A A A A A
Chain F: A A A A A A A A A A A
Chain G: A A A A A A A A A A A
Chain H: A A A A A A A A A A A
Chain I: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain J: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain K: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain L: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain M: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain N: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain O: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE
Chain P: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA LYS GLU PHE

Citation

R.C. Deo, J.B. Bonanno, N. Sonenberg, S.K. Burley
Recognition of polyadenylate RNA by the poly(A)-binding protein
Cell (Cambridge,Mass. ), 17, pp. 835-845, 1999.

Space group

P 2₁

Cell constants

     A =  79.557     B =  176.797     C =  82.489 (Ångstroms)
 alpha =  90.00   beta = 117.67  gamma =  90.00  (degrees)

Crystallization conditions

Method: vapor diffusion, hanging drop
Drop: tcep, tris-hcl, KCl, (NH4)SO4, glycerol
Reservoir: (NH4)SO4, glycerol

Refinement

The structure was refined using the CNS program. The R value is 23.0 for 57149 reflections in the resolution range 20.0 to 2.6 Å with Fobs > 2.0 sigma(Fobs).

Coordinates

The coordinates for the asymmetric unit of this structure are stored in the NDB archive.

Views of PR0007

A view of PR0007