NOTE: Homing
endonucleases do not really have recognition sequences in the way
that restriction enzymes do. The recognition sequence listed is one
site that is known to be recognized and cleaved. In general, single
base changes merely change the efficiency of cleavage and the
precise boundary of required bases is not known.
Recognition Sequence:
C T G G G T T C A A A A C G T C G T G A^G A C A G T T T G G 0 G A C C C A A G T T T T G C A G^C A C T C T G T C A A A C C 0
REBASE
enzyme #: 2540 Prototype:
I-CreI Source: J.-D.
Rochaix Microorganism: Chlamydomonasreinhardtii Growth Temperature: 26
° Enzyme gene cloned. Enzyme gene
sequenced. Crystal data
present. Comment: Insertion
site CGT^CGT Entered: Jan
1 1979 ... Modified: Sep 9 1999
