"""Command line wrapper for samtools"""
# Last Checked with samtools [0.1.20 and 1.2]
# TODO samtools 1.x has additional options over 0.x which
# are missing from this wrapper

from __future__ import print_function

from Bio.Application import _Option, _Argument, _Switch
from Bio.Application import AbstractCommandline, _ArgumentList
from Bio.Application import _StaticArgument


class SamtoolsViewCommandline(AbstractCommandline):

    """Command line wrapper for samtools view.

    Extract/print all or sub alignments in SAM or BAM format, equivalent to::

        $ samtools view [-bchuHS] [-t in.refList] [-o output] [-f reqFlag]
                        [-F skipFlag] [-q minMapQ] [-l library] [-r readGroup]
                        [-R rgFile] <in.bam>|<in.sam> [region1 [...]]

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example
    -------

    >>> from Bio.Sequencing.Applications import SamtoolsViewCommandline
    >>> input_file = "/path/to/sam_or_bam_file"
    >>> samtools_view_cmd = SamtoolsViewCommandline(input_file=input_file)
    >>> print(samtools_view_cmd)
    samtools view /path/to/sam_or_bam_file

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("view"),
            _Switch(["-b", "b"], "Output in the BAM format"),
            _Switch(["-c", "c"],
                    """Instead of printing the alignments, only count them and
                    print the total number.

                    All filter options, such as '-f', '-F' and '-q',
                    are taken into account"""),
            _Switch(["-h", "h"], "Include the header in the output"),
            _Switch(["-u", "u"],
                    """Output uncompressed BAM.

                    This option saves time spent on compression/decompression
                    and is thus preferred when the output is piped to
                    another samtools command"""),
            _Switch(["-H", "H"], "Output the header only"),
            _Switch(["-S", "S"],
                    """Input is in SAM.
                    If @SQ header lines are absent,
                    the '-t' option is required."""),
            _Option(["-t", "t"],
                    """This file is TAB-delimited.
                    Each line must contain the reference name and the
                    length of the reference, one line for each
                    distinct reference; additional fields are ignored.

                    This file also defines the order of the reference
                    sequences in sorting.
                    If you run   'samtools faidx <ref.fa>',
                    the resultant index file <ref.fa>.fai can be used
                    as this <in.ref_list> file.""",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-o", "o"], "Output file",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-f", "f"],
                    """Only output alignments with all bits in
                    INT present in the FLAG field""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-F", "F"],
                    "Skip alignments with bits present in INT",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-q", "q"],
                    "Skip alignments with MAPQ smaller than INT",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-r", "r"],
                    "Only output reads in read group STR",
                    equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-R", "R"],
                    "Output reads in read groups listed in FILE",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-l", "l"],
                    "Only output reads in library STR",
                    equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Switch(["-1", "fast_bam"],
                    "Use zlib compression level 1 to compress the output"),
            _Argument(["input", "input_file"],
                      "Input File Name", filename=True, is_required=True),
            _Argument(["region"], "Region", is_required=False),
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsMpileupCommandline(AbstractCommandline):
    """Command line wrapper for samtools mpileup.

    Generate BCF or pileup for one or multiple BAM files, equivalent to::

        $ samtools mpileup [-EBug] [-C capQcoef] [-r reg] [-f in.fa]
                           [-l list] [-M capMapQ] [-Q minBaseQ]
                           [-q minMapQ] in.bam [in2.bam [...]]

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsMpileupCommandline
    >>> input = ["/path/to/sam_or_bam_file"]
    >>> samtools_mpileup_cmd = SamtoolsMpileupCommandline(input_file=input)
    >>> print(samtools_mpileup_cmd)
    samtools mpileup /path/to/sam_or_bam_file

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("mpileup"),
            _Switch(["-E", "E"],
                    """Extended BAQ computation.
                    This option helps sensitivity especially
                    for MNPs, but may hurt specificity a little bit"""),
            _Switch(["-B", "B"],
                    """Disable probabilistic realignment for the
                    computation of base alignment quality (BAQ).

                    BAQ is the Phred-scaled probability of a read base being
                    misaligned.
                    Applying this option greatly helps to reduce false SNPs
                    caused by misalignments"""),
            _Switch(["-g", "g"],
                    """Compute genotype likelihoods and output them in the
                    binary call format (BCF)"""),
            _Switch(["-u", "u"],
                    """Similar to -g except that the output is
                    uncompressed BCF, which is preferred for piping"""),
            _Option(["-C", "C"],
                    """Coefficient for downgrading mapping quality for
                    reads containing excessive mismatches.

                    Given a read with a phred-scaled probability q of
                    being generated from the mapped position,
                    the new mapping quality is about sqrt((INT-q)/INT)*INT.
                    A zero value disables this functionality;
                    if enabled, the recommended value for BWA is 50""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-r", "r"],
                    "Only generate pileup in region STR",
                    equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-f", "f"],
                    """The faidx-indexed reference file in the FASTA format.

                    The file can be optionally compressed by razip""",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-l", "l"],
                    """BED or position list file containing a list of regions
                    or sites where pileup or BCF should be generated""",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-M", "M"],
                    "Cap Mapping Quality at M",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-q", "q"],
                    "Minimum mapping quality for an alignment to be used",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-Q", "Q"],
                    "Minimum base quality for a base to be considered",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Switch(["-6", "illumina_13"],
                    "Assume the quality is in the Illumina 1.3+ encoding"),
            _Switch(["-A", "A"],
                    "Do not skip anomalous read pairs in variant calling."),
            _Option(["-b", "b"],
                    "List of input BAM files, one file per line",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-d", "d"],
                    "At a position, read maximally INT reads per input BAM",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Switch(["-D", "D"], "Output per-sample read depth"),
            _Switch(["-S", "S"], """Output per-sample Phred-scaled
                                strand bias P-value"""),
            _Option(["-e", "e"],
                    """Phred-scaled gap extension sequencing error probability.

                    Reducing INT leads to longer indels""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-h", "h"],
                    """Coefficient for modeling homopolymer errors.

                    Given an l-long homopolymer run, the sequencing error
                    of an indel of size s is modeled as INT*s/l""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Switch(["-I", "I"], "Do not perform INDEL calling"),
            _Option(["-L", "L"],
                    """Skip INDEL calling if the average per-sample
                    depth is above INT""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-o", "o"],
                    """Phred-scaled gap open sequencing error probability.

                    Reducing INT leads to more indel calls.""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-p", "p"],
                    """Comma delimited list of platforms (determined by @RG-PL)
                    from which indel candidates are obtained.

                    It is recommended to collect indel candidates from
                    sequencing technologies that have low indel error rate
                    such as ILLUMINA""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _ArgumentList(["input_file"],
                          "Input File for generating mpileup",
                          filename=True, is_required=True),

        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsReheaderCommandline(AbstractCommandline):
    """Command line wrapper for samtools reheader.

    Replace the header in in.bam with the header
    in in.header.sam, equivalent to::

    $ samtools reheader <in.header.sam> <in.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsReheaderCommandline
    >>> input_header = "/path/to/header_sam_file"
    >>> input_bam = "/path/to/input_bam_file"
    >>> samtools_reheader_cmd = SamtoolsReheaderCommandline(\
                                    input_header=input_header,\
                                    input_bam=input_bam)
    >>> print(samtools_reheader_cmd)
    samtools reheader /path/to/header_sam_file /path/to/input_bam_file

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("reheader"),
            _Argument(["input_header", "header_sam", "sam_file"],
                      "Sam file with header",
                      filename=True, is_required=True),
            _Argument(["input_bam", "input_file", "bam_file"],
                      "BAM file for writing header to",
                      filename=True, is_required=True)
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsCatCommandline(AbstractCommandline):
    """Command line wrapper for samtools cat.

    Concatenate BAMs, equivalent to::

    $ samtools cat [-h header.sam] [-o out.bam] <in1.bam> <in2.bam> [ ... ]

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsCatCommandline
    >>> input_bam1 = "/path/to/input_bam1"
    >>> input_bam2 = "/path/to/input_bam2"
    >>> input_bams = [input_bam1, input_bam2]
    >>> samtools_cat_cmd = SamtoolsCatCommandline(input_bam=input_bams)
    >>> print(samtools_cat_cmd)
    samtools cat /path/to/input_bam1 /path/to/input_bam2

     """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("cat"),
            _Option(["-h", "h"], "Header SAM file",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-o", "o"], "Output SAM file",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _ArgumentList(["input", "input_bam", "bams"], "Input BAM files",
                          filename=True, is_required=True)

        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsSortCommandline(AbstractCommandline):
    """Command line wrapper for samtools sort.

    Concatenate BAMs, equivalent to::

    $ samtools sort [-no] [-m maxMem] <in.bam> <out.prefix>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example
    -------

    >>> from Bio.Sequencing.Applications import SamtoolsSortCommandline
    >>> input_bam = "/path/to/input_bam"
    >>> out_prefix = "/path/to/out_prefix"
    >>> samtools_sort_cmd = SamtoolsSortCommandline(\
                                                    input_bam=input_bam,\
                                                    out_prefix=out_prefix)
    >>> print(samtools_sort_cmd)
    samtools sort /path/to/input_bam /path/to/out_prefix

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("sort"),
            _Switch(["-o", "o"], """Output the final alignment
                                    to the standard output"""),
            _Switch(["-n", "n"], """Sort by read names rather
                                    than by chromosomal coordinates"""),
            _Option(["-m", "m"], "Approximately the maximum required memory",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Argument(["input_bam"], "Input BAM file",
                      filename=True, is_required=True),
            _Argument(["out_prefix"], "Output prefix",
                      filename=True, is_required=True)
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsMergeCommandline(AbstractCommandline):
    """Command line wrapper for samtools merge.

    Merge multiple sorted alignments, equivalent to::

        $ samtools merge [-nur1f] [-h inh.sam] [-R reg]
                         <out.bam> <in1.bam> <in2.bam> [...]

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example
    -------

    >>> from Bio.Sequencing.Applications import SamtoolsMergeCommandline
    >>> out_bam = "/path/to/out_bam"
    >>> in_bam = ["/path/to/input_bam1", "/path/to/input_bam2"]
    >>> samtools_merge_cmd = SamtoolsMergeCommandline(\
                                                      out_bam=out_bam,\
                                                      input_bam=in_bam)
    >>> print(samtools_merge_cmd)
    samtools merge /path/to/out_bam /path/to/input_bam1 /path/to/input_bam2

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("merge"),
            _Switch(["-n", "n"],
                    """The input alignments are sorted by read names
                    rather than by chromosomal coordinates"""),
            _Switch(["-r", "r"], """Attach an RG tag to each alignment.
                    The tag value is inferred from file names"""),
            _Switch(["-u", "u"], "Uncompressed BAM output"),
            _Switch(["-1", "fast_bam"], """Use zlib compression level 1
                                           to compress the output"""),
            _Switch(["-f", "f"], """Force to overwrite the
                                    output file if present"""),
            _Option(["-h", "h"], """Use the lines of FILE as '@'
                                    headers to be copied to out.bam""",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-R", "R"],
                    "Merge files in the specified region indicated by STR",
                    equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Argument(["output_bam", "out_bam", "out", "output"],
                      "Output BAM file",
                      filename=True, is_required=True),
            _ArgumentList(["input_bam", "in_bam", "input", "bam"],
                          "Input BAM",
                          filename=True, is_required=True),
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsIndexCommandline(AbstractCommandline):
    """Command line wrapper for samtools index.

    Index sorted alignment for fast random access, equivalent to::

    $ samtools index <aln.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsIndexCommandline
    >>> input = "/path/to/aln_bam"
    >>> samtools_index_cmd = SamtoolsIndexCommandline(input_bam=input)
    >>> print(samtools_index_cmd)
    samtools index /path/to/aln_bam

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("index"),
            _Argument(["input", "in_bam", "input_bam"],
                      "BAM file to be indexed"),
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsIdxstatsCommandline(AbstractCommandline):
    """Command line wrapper for samtools idxstats.

    Retrieve and print stats in the index file, equivalent to::

    $ samtools idxstats <aln.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsIdxstatsCommandline
    >>> input = "/path/to/aln_bam"
    >>> samtools_idxstats_cmd = SamtoolsIdxstatsCommandline(input_bam=input)
    >>> print(samtools_idxstats_cmd)
    samtools idxstats /path/to/aln_bam

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("idxstats"),
            _Argument(["input", "in_bam", "input_bam"],
                      "BAM file to be indexed")
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsFaidxCommandline(AbstractCommandline):
    """Command line wrapper for samtools faidx.

    Retrieve and print stats in the index file, equivalent to::

    $ samtools faidx <ref.fasta> [region1 [...]]

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example
    -------

    >>> from Bio.Sequencing.Applications import SamtoolsFaidxCommandline
    >>> reference = "/path/to/reference.fasta"
    >>> samtools_faidx_cmd = SamtoolsFaidxCommandline(reference=reference)
    >>> print(samtools_faidx_cmd)
    samtools faidx /path/to/reference.fasta

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("faidx"),
            _Argument(["reference", "reference_fasta", "ref"],
                      "Reference FASTA to be indexed",
                      filename=True, is_required=True)

        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsFixmateCommandline(AbstractCommandline):
    """Command line wrapper for samtools fixmate.

    Fill in mate coordinates, ISIZE and mate related
    flags from a name-sorted alignment, equivalent to::

    $ samtools fixmate <in.nameSrt.bam> <out.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsFixmateCommandline
    >>> in_bam = "/path/to/in.nameSrt.bam"
    >>> out_bam = "/path/to/out.bam"
    >>> samtools_fixmate_cmd = SamtoolsFixmateCommandline(\
                                                          input_bam=in_bam,\
                                                          out_bam=out_bam)
    >>> print(samtools_fixmate_cmd)
    samtools fixmate /path/to/in.nameSrt.bam /path/to/out.bam

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("fixmate"),
            _Argument(["in_bam", "sorted_bam", "input_bam",
                       "input", "input_file"],
                      "Name Sorted Alignment File ",
                      filename=True, is_required=True),
            _Argument(["out_bam", "output_bam", "output", "output_file"],
                      "Output file",
                      filename=True, is_required=True)
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsRmdupCommandline(AbstractCommandline):
    """Command line wrapper for samtools rmdup.

    Remove potential PCR duplicates, equivalent to::

    $ samtools rmdup [-sS] <input.srt.bam> <out.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsRmdupCommandline
    >>> input_sorted_bam = "/path/to/input.srt.bam"
    >>> out_bam = "/path/to/out.bam"
    >>> samtools_rmdup_cmd = SamtoolsRmdupCommandline(\
                                                      input_bam=input_sorted_bam,\
                                                      out_bam=out_bam)
    >>> print(samtools_rmdup_cmd)
    samtools rmdup /path/to/input.srt.bam /path/to/out.bam

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("rmdup"),
            _Switch(["-s", "s"],
                    """Remove duplicates for single-end reads.

                    By default, the command works for paired-end
                    reads only"""),
            _Switch(["-S", "S"], """Treat paired-end reads
                                    as single-end reads"""),
            _Argument(["in_bam", "sorted_bam", "input_bam",
                       "input", "input_file"],
                      "Name Sorted Alignment File ",
                      filename=True, is_required=True),
            _Argument(["out_bam", "output_bam", "output", "output_file"],
                      "Output file", filename=True, is_required=True)

        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsCalmdCommandline(AbstractCommandline):
    """Command line wrapper for samtools calmd.

    Generate the MD tag, equivalent to::

    $ samtools calmd [-EeubSr] [-C capQcoef] <aln.bam> <ref.fasta>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsCalmdCommandline
    >>> input_bam = "/path/to/aln.bam"
    >>> reference_fasta = "/path/to/reference.fasta"
    >>> samtools_calmd_cmd = SamtoolsCalmdCommandline(\
                                                      input_bam=input_bam,\
                                                      reference=reference_fasta)
    >>> print(samtools_calmd_cmd)
    samtools calmd /path/to/aln.bam /path/to/reference.fasta

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("calmd"),
            _Switch(["-E", "E"],
                    """Extended BAQ calculation.
                    This option trades specificity for sensitivity,
                    though the effect is minor."""),
            _Switch(["-e", "e"],
                    """Convert the read base to = if it is
                    identical to the aligned reference base.

                    Indel caller does not support the = bases
                    at the moment."""),
            _Switch(["-u", "u"], "Output uncompressed BAM"),
            _Switch(["-b", "b"], "Output compressed BAM "),
            _Switch(["-S", "S"], "The input is SAM with header lines "),
            _Switch(["-r", "r"], """Compute the BQ tag (without -A)
                    or cap base quality by BAQ (with -A)."""),
            _Switch(["-A", "A"],
                    """When used jointly with -r this option overwrites
                    the original base quality"""),
            _Option(["-C", "C"], """Coefficient to cap mapping quality
                    of poorly mapped reads.

                    See the pileup command for details.""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Argument(["input", "input_file", "in_bam", "infile", "input_bam"],
                      "Input BAM", filename=True, is_required=True),
            _Argument(["reference", "reference_fasta", "ref"],
                      "Reference FASTA to be indexed",
                      filename=True, is_required=True)

        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsTargetcutCommandline(AbstractCommandline):
    """Command line wrapper for samtools targetcut.

    This command identifies target regions by examining the continuity
    of read depth, computes haploid consensus sequences of targets
    and outputs a SAM with each sequence corresponding to a target,
    equivalent to::

        $ samtools targetcut [-Q minBaseQ] [-i inPenalty] [-0 em0]
                             [-1 em1] [-2 em2] [-f ref] <in.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsTargetcutCommandline
    >>> input_bam = "/path/to/aln.bam"
    >>> samtools_targetcut_cmd = SamtoolsTargetcutCommandline(input_bam=input_bam)
    >>> print(samtools_targetcut_cmd)
    samtools targetcut /path/to/aln.bam

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("targetcut"),
            _Option(["-Q", "Q"], "Minimum Base Quality ",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-i", "i"], "Insertion Penalty",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-f", "f"], "Reference Filename",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-0", "em0"], "em0", equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-1", "em1"], "em1", equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Option(["-2", "em2"], "em2", equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Argument(["input", "input_bam", "in_bam"],
                      "Input file",
                      filename=True, is_required=True)

        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


class SamtoolsPhaseCommandline(AbstractCommandline):
    """Command line wrapper for samtools phase.

    Call and phase heterozygous SNPs, equivalent to::

        $ samtools phase [-AF] [-k len] [-b prefix]
                         [-q minLOD] [-Q minBaseQ] <in.bam>

    See http://samtools.sourceforge.net/samtools.shtml for more details

    Example:

    >>> from Bio.Sequencing.Applications import SamtoolsPhaseCommandline
    >>> input_bam = "/path/to/in.bam"
    >>> samtools_phase_cmd = SamtoolsPhaseCommandline(input_bam=input_bam)
    >>> print(samtools_phase_cmd)
    samtools phase /path/to/in.bam

    """
    def __init__(self, cmd="samtools", **kwargs):
        self.program_name = cmd
        self.parameters = [
            _StaticArgument("phase"),
            _Argument(["input", "input_bam", "in_bam"], "Input file",
                      filename=True, is_required=True),
            _Switch(["-A", "A"], "Drop reads with ambiguous phase"),
            _Option(["-b", "b"], "Prefix of BAM output",
                    filename=True, equate=False,
                    checker_function=lambda x: isinstance(x, str)),
            _Switch(["-F", "F"], "Do not attempt to fix chimeric reads"),
            _Option(["-k", "k"], "Maximum length for local phasing",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-q", "q"], """Minimum Phred-scaled LOD to
                    call a heterozygote""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int)),
            _Option(["-Q", "Q"], """Minimum base quality to be
                    used in het calling""",
                    equate=False,
                    checker_function=lambda x: isinstance(x, int))
        ]
        AbstractCommandline.__init__(self, cmd, **kwargs)


if __name__ == "__main__":
    from Bio._utils import run_doctest
    run_doctest()