Package: centrifuge / 1.0.3-8

2to3.patch Patch series | download
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Description: Use 2to3 to port to Python3
Bug-Debian: https://bugs.debian.org/936281
Author: Andreas Tille <tille@debian.org>
Last-Update: Tue, 10 Sep 2019 08:02:24 +0200

--- a/Makefile
+++ b/Makefile
@@ -364,11 +364,11 @@ centrifuge.bat:
 
 centrifuge-build.bat:
 	echo "@echo off" > centrifuge-build.bat
-	echo "python %~dp0/centrifuge-build %*" >> centrifuge-build.bat
+	echo "python3 %~dp0/centrifuge-build %*" >> centrifuge-build.bat
 
 centrifuge-inspect.bat:
 	echo "@echo off" > centrifuge-inspect.bat
-	echo "python %~dp0/centrifuge-inspect %*" >> centrifuge-inspect.bat
+	echo "python3 %~dp0/centrifuge-inspect %*" >> centrifuge-inspect.bat
 
 
 .PHONY: centrifuge-src
--- a/centrifuge-build
+++ b/centrifuge-build
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/python3
 
 """
  Copyright 2014, Daehwan Kim <infphilo@gmail.com>
--- a/centrifuge-inspect
+++ b/centrifuge-inspect
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/python3
 
 """
  Copyright 2014, Daehwan Kim <infphilo@gmail.com>
--- a/evaluation/centrifuge_evaluate.py
+++ b/evaluation/centrifuge_evaluate.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/python3
 
 import sys, os, subprocess, inspect
 import platform, multiprocessing
@@ -25,7 +25,7 @@ def read_taxonomy_tree(tax_file):
 """
 def compare_scm(centrifuge_out, true_out, taxonomy_tree, rank):
     ancestors = set()
-    for tax_id in taxonomy_tree.keys():
+    for tax_id in list(taxonomy_tree.keys()):
         if tax_id in ancestors:
             continue
         while True:
@@ -106,7 +106,7 @@ def compare_scm(centrifuge_out, true_out
             unclassified += 1
 
     raw_unique_classified = 0
-    for value in db_dic.values():
+    for value in list(db_dic.values()):
         if len(value) == 1:
             raw_unique_classified += 1
     return classified, unique_classified, unclassified, len(db_dic), raw_unique_classified
@@ -152,7 +152,7 @@ def compare_abundance(centrifuge_out, tr
         if tax_id in db_dic:
             SSR += (abundance - db_dic[tax_id]) ** 2;
             if debug:
-                print >> sys.stderr, "\t\t\t\t{:<10}: {:.6} vs. {:.6} (truth vs. centrifuge)".format(tax_id, abundance, db_dic[tax_id])
+                print("\t\t\t\t{:<10}: {:.6} vs. {:.6} (truth vs. centrifuge)".format(tax_id, abundance, db_dic[tax_id]), file=sys.stderr)
         else:
             SSR += (abundance) ** 2
 
@@ -179,7 +179,7 @@ def sql_execute(sql_db, sql_query):
 """
 def create_sql_db(sql_db):
     if os.path.exists(sql_db):
-        print >> sys.stderr, sql_db, "already exists!"
+        print(sql_db, "already exists!", file=sys.stderr)
         return
     
     columns = [
@@ -316,7 +316,7 @@ def evaluate(index_base,
         os.mkdir(index_path)
     index_fnames = ["%s/%s.%d.cf" % (index_path, index_base, i+1) for i in range(3)]
     if not check_files(index_fnames):
-        print >> sys.stderr, "Downloading indexes: %s" % ("index")
+        print("Downloading indexes: %s" % ("index"), file=sys.stderr)
         os.system("cd %s; wget ftp://ftp.ccb.jhu.edu/pub/infphilo/centrifuge/data/%s.tar.gz; tar xvzf %s.tar.gz; rm %s.tar.gz; ln -s %s/%s* .; cd -" % \
                       (index_path, index_base, index_base, index_base, index_base, index_base))
         assert check_files(index_fnames)        
@@ -356,7 +356,7 @@ def evaluate(index_base,
     scm_fname = "%s/%s.scm" % (read_path, read_base)
     read_fnames = [read1_fname, read2_fname, truth_fname, scm_fname]
     if not check_files(read_fnames):
-        print >> sys.stderr, "Simulating reads %s_1.fq %s_2.fq ..." % (read_base, read_base)
+        print("Simulating reads %s_1.fq %s_2.fq ..." % (read_base, read_base), file=sys.stderr)
         centrifuge_simulate = os.path.join(path_base, "centrifuge_simulate_reads.py")
         simulate_cmd = [centrifuge_simulate,
                         "--num-fragment", str(num_fragment)]
@@ -377,11 +377,11 @@ def evaluate(index_base,
     else:
         base_fname = read_base + "_single"
 
-    print >> sys.stderr, "Database: %s" % (index_base)
+    print("Database: %s" % (index_base), file=sys.stderr)
     if paired:
-        print >> sys.stderr, "\t%d million pairs" % (num_fragment / 1000000)
+        print("\t%d million pairs" % (num_fragment / 1000000), file=sys.stderr)
     else:
-        print >> sys.stderr, "\t%d million reads" % (num_fragment / 1000000)
+        print("\t%d million reads" % (num_fragment / 1000000), file=sys.stderr)
 
     program_bin_base = "%s/.." % path_base
     def get_program_version(program, version):
@@ -428,7 +428,7 @@ def evaluate(index_base,
         if version:
             program_name += ("_%s" % version)
 
-        print >> sys.stderr, "\t%s\t%s" % (program_name, str(datetime.now()))
+        print("\t%s\t%s" % (program_name, str(datetime.now())), file=sys.stderr)
         if paired:
             program_dir = program_name + "_paired"
         else:
@@ -449,7 +449,7 @@ def evaluate(index_base,
         program_cmd = get_program_cmd(program, version, read1_fname, read2_fname, out_fname)
         start_time = datetime.now()
         if verbose:
-            print >> sys.stderr, "\t", start_time, " ".join(program_cmd)
+            print("\t", start_time, " ".join(program_cmd), file=sys.stderr)
         if program in ["centrifuge"]:
             proc = subprocess.Popen(program_cmd, stdout=open(out_fname, "w"), stderr=subprocess.PIPE)
         else:
@@ -462,7 +462,7 @@ def evaluate(index_base,
         if duration < 0.1:
             duration = 0.1
         if verbose:
-            print >> sys.stderr, "\t", finish_time, "finished:", duration            
+            print("\t", finish_time, "finished:", duration, file=sys.stderr)            
 
         results = {"strain"  : [0, 0, 0],
                    "species" : [0, 0, 0],
@@ -484,21 +484,21 @@ def evaluate(index_base,
             # if rank == "strain":
             #    assert num_cases == num_fragment
 
-            print >> sys.stderr, "\t\t%s" % rank
-            print >> sys.stderr, "\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(classified, num_cases, float(classified) / num_cases)
-            print >> sys.stderr, "\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(classified, raw_classified, float(classified) / raw_classified)
-            print >> sys.stderr, "\n\t\t\tfor uniquely classified ",
+            print("\t\t%s" % rank, file=sys.stderr)
+            print("\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(classified, num_cases, float(classified) / num_cases), file=sys.stderr)
+            print("\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(classified, raw_classified, float(classified) / raw_classified), file=sys.stderr)
+            print("\n\t\t\tfor uniquely classified ", end=' ', file=sys.stderr)
             if paired:
-                print >> sys.stderr, "pairs"
+                print("pairs", file=sys.stderr)
             else:
-                print >> sys.stderr, "reads"
-            print >> sys.stderr, "\t\t\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(unique_classified, num_cases, float(unique_classified) / num_cases)
-            print >> sys.stderr, "\t\t\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(unique_classified, raw_unique_classified, float(unique_classified) / raw_unique_classified)
+                print("reads", file=sys.stderr)
+            print("\t\t\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(unique_classified, num_cases, float(unique_classified) / num_cases), file=sys.stderr)
+            print("\t\t\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(unique_classified, raw_unique_classified, float(unique_classified) / raw_unique_classified), file=sys.stderr)
 
             # Calculate sum of squared residuals in abundance
             if rank == "strain":
                 abundance_SSR = compare_abundance("centrifuge_report.tsv", truth_fname, taxonomy_tree, debug)
-                print >> sys.stderr, "\t\t\tsum of squared residuals in abundance: {}".format(abundance_SSR)
+                print("\t\t\tsum of squared residuals in abundance: {}".format(abundance_SSR), file=sys.stderr)
 
         if runtime_only:
             os.chdir("..")
--- a/evaluation/centrifuge_simulate_reads.py
+++ b/evaluation/centrifuge_simulate_reads.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/python3
 
 #
 # Copyright 2015, Daehwan Kim <infphilo@gmail.com>
@@ -156,7 +156,7 @@ def read_transcript(genomes_seq, gtf_fil
             transcripts[transcript_id][2].append([left, right])
 
     # Sort exons and merge where separating introns are <=5 bps
-    for tran, [chr, strand, exons] in transcripts.items():
+    for tran, [chr, strand, exons] in list(transcripts.items()):
             exons.sort()
             tmp_exons = [exons[0]]
             for i in range(1, len(exons)):
@@ -167,7 +167,7 @@ def read_transcript(genomes_seq, gtf_fil
             transcripts[tran] = [chr, strand, tmp_exons]
 
     tmp_transcripts = {}
-    for tran, [chr, strand, exons] in transcripts.items():
+    for tran, [chr, strand, exons] in list(transcripts.items()):
         exon_lens = [e[1] - e[0] + 1 for e in exons]
         transcript_len = sum(exon_lens)
         if transcript_len >= frag_len:
@@ -444,8 +444,8 @@ def getSamAlignment(dna, exons, genome_s
         MD += ("{}".format(MD_match_len))
 
     if len(read_seq) != read_len:
-        print >> sys.stderr, "read length differs:", len(read_seq), "vs.", read_len
-        print >> sys.stderr, pos, "".join(cigars), cigar_descs, MD, XM, NM, Zs
+        print("read length differs:", len(read_seq), "vs.", read_len, file=sys.stderr)
+        print(pos, "".join(cigars), cigar_descs, MD, XM, NM, Zs, file=sys.stderr)
         assert False
 
     return pos, cigars, cigar_descs, MD, XM, NM, Zs, read_seq
@@ -575,8 +575,8 @@ def samRepOk(genome_seq, read_seq, chr,
         tMD += ("{}".format(match_len))
 
     if tMD != MD or tXM != XM or tNM != NM or XM > max_mismatch or XM != NM:
-        print >> sys.stderr, chr, pos, cigar, MD, XM, NM, Zs
-        print >> sys.stderr, tMD, tXM, tNM
+        print(chr, pos, cigar, MD, XM, NM, Zs, file=sys.stderr)
+        print(tMD, tXM, tNM, file=sys.stderr)
         assert False
         
         
@@ -631,7 +631,7 @@ def simulate_reads(index_fname, base_fna
     # Read genome sequences into memory
     genomes_fname = index_fname + ".fa"
     if not os.path.exists(genomes_fname):
-        print >> sys.stderr, "Extracting genomes from Centrifuge index to %s, which may take a few hours ..."  % (genomes_fname)
+        print("Extracting genomes from Centrifuge index to %s, which may take a few hours ..."  % (genomes_fname), file=sys.stderr)
         extract_cmd = [centrifuge_inspect,
                        index_fname]
         extract_proc = subprocess.Popen(extract_cmd, stdout=open(genomes_fname, 'w'))
@@ -660,15 +660,15 @@ def simulate_reads(index_fname, base_fna
     assert num_frag == sum(expr_profile)
 
     if dna:
-        genome_ids = genome_seqs.keys()
+        genome_ids = list(genome_seqs.keys())
     else:
-        transcript_ids = transcripts.keys()
+        transcript_ids = list(transcripts.keys())
         random.shuffle(transcript_ids)
         assert len(transcript_ids) >= len(expr_profile)
 
     # Truth table
     truth_file = open(base_fname + ".truth", "w")
-    print >> truth_file, "taxID\tgenomeLen\tnumReads\tabundance\tname"
+    print("taxID\tgenomeLen\tnumReads\tabundance\tname", file=truth_file)
     truth_list = []
     normalized_sum = 0.0
     debug_num_frag = 0
@@ -695,19 +695,19 @@ def simulate_reads(index_fname, base_fna
         if can_tax_id in names:
             name = names[can_tax_id]
         abundance = raw_abundance / genome_len / normalized_sum
-        print >> truth_file, "{}\t{}\t{}\t{:.6}\t{}".format(tax_id, genome_len, t_num_frags, abundance, name)
+        print("{}\t{}\t{}\t{:.6}\t{}".format(tax_id, genome_len, t_num_frags, abundance, name), file=truth_file)
     truth_file.close()
 
     # Sequence Classification Map (SCM) - something I made up ;-)
     scm_file = open(base_fname + ".scm", "w")
 
     # Write SCM header
-    print >> scm_file, "@HD\tVN:1.0\tSO:unsorted"
-    for tax_id in genome_seqs.keys():
+    print("@HD\tVN:1.0\tSO:unsorted", file=scm_file)
+    for tax_id in list(genome_seqs.keys()):
         name = ""
         if tax_id in names:
             name = names[tax_id]
-        print >> scm_file, "@SQ\tTID:%s\tSN:%s\tLN:%d" % (tax_id, name, len(genome_seqs[tax_id]))
+        print("@SQ\tTID:%s\tSN:%s\tLN:%d" % (tax_id, name, len(genome_seqs[tax_id])), file=scm_file)
 
     read_file = open(base_fname + "_1.fa", "w")
     if paired_end:
@@ -718,11 +718,11 @@ def simulate_reads(index_fname, base_fna
         t_num_frags = expr_profile[t]
         if dna:
             tax_id = genome_ids[t]
-            print >> sys.stderr, "TaxID: %s, num fragments: %d" % (tax_id, t_num_frags)
+            print("TaxID: %s, num fragments: %d" % (tax_id, t_num_frags), file=sys.stderr)
         else:
             transcript_id = transcript_ids[t]
             chr, strand, transcript_len, exons = transcripts[transcript_id]
-            print >> sys.stderr, transcript_id, t_num_frags
+            print(transcript_id, t_num_frags, file=sys.stderr)
 
         genome_seq = genome_seqs[tax_id]
         genome_len = len(genome_seq)
@@ -763,14 +763,14 @@ def simulate_reads(index_fname, base_fna
                 XS = "\tXS:A:{}".format(strand)
                 TI = "\tTI:Z:{}".format(transcript_id)                
 
-            print >> read_file, ">{}".format(cur_read_id)
-            print >> read_file, read_seq
+            print(">{}".format(cur_read_id), file=read_file)
+            print(read_seq, file=read_file)
             output = "{}\t{}\t{}\t{}\tNM:i:{}\tMD:Z:{}".format(cur_read_id, tax_id, pos + 1, cigar_str, NM, MD)
             if paired_end:
-                print >> read2_file, ">{}".format(cur_read_id)
-                print >> read2_file, reverse_complement(read2_seq)
+                print(">{}".format(cur_read_id), file=read2_file)
+                print(reverse_complement(read2_seq), file=read2_file)
                 output += "\t{}\t{}\tNM2:i:{}\tMD2:Z:{}".format(pos2 + 1, cigar2_str, NM2, MD2)
-            print >> scm_file, output
+            print(output, file=scm_file)
                 
             cur_read_id += 1
             
@@ -865,7 +865,7 @@ if __name__ == '__main__':
         parser.print_help()
         exit(1)
     if not args.dna:
-        print >> sys.stderr, "Error: --rna is not implemented."
+        print("Error: --rna is not implemented.", file=sys.stderr)
         exit(1)
     # if args.dna:
     #    args.expr_profile = "constant"
--- a/evaluation/test/centrifuge_evaluate_mason.py
+++ b/evaluation/test/centrifuge_evaluate_mason.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/python3
 
 import sys, os, subprocess, inspect
 import platform, multiprocessing
@@ -27,7 +27,7 @@ def compare_scm(centrifuge_out, true_out
     higher_ranked = {}
         
     ancestors = set()
-    for tax_id in taxonomy_tree.keys():
+    for tax_id in list(taxonomy_tree.keys()):
         if tax_id in ancestors:
             continue
         while True:
@@ -82,7 +82,7 @@ def compare_scm(centrifuge_out, true_out
 
         fields = line.strip().split('\t')
         if len(fields) != 3:
-            print >> sys.stderr, "Warning: %s missing" % (line.strip())
+            print("Warning: %s missing" % (line.strip()), file=sys.stderr)
             continue
         read_name, tax_id = fields[1:3] 
         # Traverse up taxonomy tree to match the given rank parameter
@@ -117,7 +117,7 @@ def compare_scm(centrifuge_out, true_out
             # print read_name
 
     raw_unique_classified = 0
-    for read_name, maps in db_dic.items():
+    for read_name, maps in list(db_dic.items()):
         if len(maps) == 1 and read_name not in higher_ranked:
             raw_unique_classified += 1
     return classified, unique_classified, unclassified, len(db_dic), raw_unique_classified
@@ -184,7 +184,7 @@ def evaluate(index_base,
                       read_fname]
 
     if verbose:
-        print >> sys.stderr, ' '.join(centrifuge_cmd)
+        print(' '.join(centrifuge_cmd), file=sys.stderr)
 
     out_fname = "centrifuge.output"
     proc = subprocess.Popen(centrifuge_cmd, stdout=open(out_fname, "w"), stderr=subprocess.PIPE)
@@ -208,12 +208,12 @@ def evaluate(index_base,
         # if rank == "strain":
         #    assert num_cases == num_fragment
 
-        print >> sys.stderr, "\t\t%s" % rank
-        print >> sys.stderr, "\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(classified, num_cases, float(classified) / num_cases)
-        print >> sys.stderr, "\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(classified, raw_classified, float(classified) / raw_classified)
-        print >> sys.stderr, "\n\t\t\tfor uniquely classified "
-        print >> sys.stderr, "\t\t\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(unique_classified, num_cases, float(unique_classified) / num_cases)
-        print >> sys.stderr, "\t\t\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(unique_classified, raw_unique_classified, float(unique_classified) / raw_unique_classified)
+        print("\t\t%s" % rank, file=sys.stderr)
+        print("\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(classified, num_cases, float(classified) / num_cases), file=sys.stderr)
+        print("\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(classified, raw_classified, float(classified) / raw_classified), file=sys.stderr)
+        print("\n\t\t\tfor uniquely classified ", file=sys.stderr)
+        print("\t\t\t\t\tsensitivity: {:,} / {:,} ({:.2%})".format(unique_classified, num_cases, float(unique_classified) / num_cases), file=sys.stderr)
+        print("\t\t\t\t\tprecision  : {:,} / {:,} ({:.2%})".format(unique_classified, raw_unique_classified, float(unique_classified) / raw_unique_classified), file=sys.stderr)
 
         # Calculate sum of squared residuals in abundance
         """
@@ -252,12 +252,12 @@ def evaluate(index_base,
         if rank_taxID not in true_abundance:
             true_abundance[rank_taxID] = 0.0
         true_abundance[rank_taxID] += (reads / float(genomeSize))
-    for taxID, reads in true_abundance.items():
+    for taxID, reads in list(true_abundance.items()):
         true_abundance[taxID] /= total_sum
 
-    print >> sys.stderr, "number of genomes:", num_genomes
-    print >> sys.stderr, "number of species:", num_species
-    print >> sys.stderr, "number of uniq species:", len(true_abundance)
+    print("number of genomes:", num_genomes, file=sys.stderr)
+    print("number of species:", num_species, file=sys.stderr)
+    print("number of uniq species:", len(true_abundance), file=sys.stderr)
 
     read_fname = "centrifuge_data/bacteria_sim10M/bacteria_sim10M.fa"
     summary_fname = "centrifuge.summary"
@@ -271,14 +271,14 @@ def evaluate(index_base,
                       read_fname]
 
     if verbose:
-        print >> sys.stderr, ' '.join(centrifuge_cmd)
+        print(' '.join(centrifuge_cmd), file=sys.stderr)
 
     out_fname = "centrifuge.output"
     proc = subprocess.Popen(centrifuge_cmd, stdout=open(out_fname, "w"), stderr=subprocess.PIPE)
     proc.communicate()
 
     calc_abundance = {}
-    for taxID in true_abundance.keys():
+    for taxID in list(true_abundance.keys()):
         calc_abundance[taxID] = 0.0
     first = True
     for line in open(summary_fname):
@@ -296,12 +296,12 @@ def evaluate(index_base,
         """
 
     abundance_file = open("abundance.cmp", 'w')
-    print >> abundance_file, "taxID\ttrue\tcalc\trank"
+    print("taxID\ttrue\tcalc\trank", file=abundance_file)
     for rank in ranks:
         if rank == "strain":
             continue
         true_abundance_rank, calc_abundance_rank = {}, {}
-        for taxID in true_abundance.keys():
+        for taxID in list(true_abundance.keys()):
             assert taxID in calc_abundance
             rank_taxID = taxID
             while True:
@@ -322,11 +322,11 @@ def evaluate(index_base,
             calc_abundance_rank[rank_taxID] += calc_abundance[taxID]
 
         ssr = 0.0 # Sum of Squared Residuals
-        for taxID in true_abundance_rank.keys():
+        for taxID in list(true_abundance_rank.keys()):
             assert taxID in calc_abundance_rank
             ssr += (true_abundance_rank[taxID] - calc_abundance_rank[taxID]) ** 2
-            print >> abundance_file, "%s\t%.6f\t%.6f\t%s" % (taxID, true_abundance_rank[taxID], calc_abundance_rank[taxID], rank)
-        print >> sys.stderr, "%s) Sum of squared residuals: %.6f" % (rank, ssr)
+            print("%s\t%.6f\t%.6f\t%s" % (taxID, true_abundance_rank[taxID], calc_abundance_rank[taxID], rank), file=abundance_file)
+        print("%s) Sum of squared residuals: %.6f" % (rank, ssr), file=sys.stderr)
     abundance_file.close()