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|
Author: Steffen Moeller <moeller@debian.org>
LastChanged: Sat, 12 Jun 2010 16:03:22 +0200
Description:
* coils-wrap now uses Getopt::Long
* coils-wrap now also writes out raw prediction
* coils-wrap input file format recognition improved
* ncoils.c does not require the env var COILSDIR defined any more
* -max_seq_len is not a cmd line argument any more
* added coils-wrap.pod and ncoils.pod
--- a/coils-wrap.pl
+++ b/coils-wrap.pl
@@ -1,6 +1,8 @@
-#!/usr/local/bin/perl
-
+#!/usr/bin/perl -w
$|=1;
+use Carp qw| cluck :DEFAULT |;
+use Getopt::Long;
+use File::Temp qw||;
# Runs coils multiply to give you a three-window output
@@ -9,42 +11,75 @@ $|=1;
# You may need to change these, in addition
# to the "/usr/local/bin/perl" above if necessary
#
-$ENV{"COILSDIR"} = "/data/ATG/coils/";
-$coils_root = "/data/ATG/coils/";
-$coils_exec = "/apps/ATG/bin/coils";
+#$ENV{"COILSDIR"} = "__MAKE_PREFIX__/usr/share/coiledcoils";
+$coils_root = "__pkgdatadir__";
+$coils_exec = "ncoils";
-$tmpdir = "/tmp/";
+$tmpdir = File::Temp::tempdir( CLEANUP => 1 );
undef($in_seq);
undef($id);
$mode = "threewin";
$weighted = "";
$matrix = "MTK";
-$win = "21";
+#$win = "21";
$extra = "";
-for($i=0; $i<=$#ARGV; ++$i) {
- if($ARGV[$i] eq "-m") { # matrix file
- if(($i+1)>($#ARGV)) { exit_error(); }
- $matrix = uc($ARGV[$i+1]);
- $i++;
- } elsif($ARGV[$i] eq "-w") { # matrix file
- $weighted = " -w ";
- } elsif(!defined($fastafile)) {
- $fastafile = $ARGV[$i];
- } else {
- exit_error();
- }
+$nameScr = $0;
+$nameScr =~ s/.*\///g;
+
+$Lok = GetOptions ("m=s" => \$matrix,
+ "w!" => \$opt_weight,
+ "i=s" => \$fastafile,
+ "o=s" => \$outfile,
+ "r=s" => \$fileRaw,
+ "debug!" => \$dbg
+ #'help' => \$opt_help);
+ );
+
+
+if ( ! $Lok ) {
+ print STDERR
+ "Invalid arguments found, -h or --help for help\n",
+ "Usage: $nameScr [options] -i fastafile -o out_file -r raw_file\n",
+ " -m [matrix type] set matrix (MTK or MTIDK)\n",
+ " -w weight a&d = b,c,e,f&g\n",
+ "Try $nameScr --help for more information\n";
+
+ exit(1);
+}
+
+
+if ( ! $fastafile or ! $outfile ) {
+ print STDERR
+ "Usage: $nameScr [options] -i fastafile -o out_file -r raw_file\n",
+ " -m [matrix type] set matrix (MTK or MTIDK)\n",
+ " -w weight a&d = b,c,e,f&g\n",
+ "Try $nameScr --help for more information\n";
+ exit(1);
+}
+
+if ( ! -f $fastafile ) {
+ print STDERR
+ "input file '$fastafile' not found, exiting..\n";
+ exit(1);
+}
+
+$fileRaw = $outfile."_raw" if ( ! defined $fileRaw );
+
+$matrix = uc($matrix);
+if ( $opt_weight ) {
+ $weighted = " -w ";
}
sub exit_error {
- print STDERR "coils-wrap.pl [options] [fasta file]\n";
+ print STDERR "coils-wrap.pl [options] [fasta file] [output file]\n";
print STDERR " -m [matrix type] set matrix (MTK or MTIDK)\n";
print STDERR " -w weight a&d = b,c,e,f&g\n";
exit;
}
-@data = read_align($fastafile,$type);
+@data = read_align($fastafile,\$type);
if($type ne "f") {
die "Error file must be in FASTA format\n";
}
@@ -61,14 +96,14 @@ $seqfile = $tmpdir . "coils." . $$ . ".f
$seqs = get_afasta(@data);
-print "What should appear below is the result of running COILS three \n";
-print "times with windows of 14,21 & 28.\n`frame' denotes the predicted \n";
-print "position of the heptad repeat for the highest scoring window that \n";
-print "overlaps each position.\n`prob' gives a single integer value \n";
-print "representation of P (where 0-9 covers the range 0.0-1.0)\n";
-print "This will be repeated for each sequence \n\n\n";
+#print "What should appear below is the result of running COILS three \n";
+#print "times with windows of 14,21 & 28.\n`frame' denotes the predicted \n";
+#print "position of the heptad repeat for the highest scoring window that \n";
+#print "overlaps each position.\n`prob' gives a single integer value \n";
+#print "representation of P (where 0-9 covers the range 0.0-1.0)\n";
+#print "This will be repeated for each sequence \n\n\n";
-print "A total of $seqs->{nseq} sequences found in $seqfile\n\n\n";
+#print "A total of $seqs->{nseq} sequences found in $seqfile\n\n\n";
for($n=0; $n<$seqs->{nseq}; ++$n) {
$id = $seqs->{list}[$n];
$in_seq = $seqs->{ids}{$id}{seq};
@@ -76,7 +111,8 @@ for($n=0; $n<$seqs->{nseq}; ++$n) {
# print "ID $id seq $seq\n";
&write_fasta($seqfile,$id,$in_seq);
-
+ @result = ();
+ push @result, ">$id\n\n";
if($mode eq "threewin") {
# Runs threewin style mode
# Three windows 14,21,28
@@ -94,7 +130,10 @@ for($n=0; $n<$seqs->{nseq}; ++$n) {
for($i=14; $i<=28; $i+=7) {
$j=($i-14)/7;
$command = $coils_exec . $extra . " -win " . $i . " < " . $seqfile;
-# print "Command is $command\n";
+ if ( $i == 28 ) {
+ open (RAW,">$fileRaw") or die "cannot write to $fileRaw:$!";
+ }
+ if($dbg){ cluck("Command is $command"); }
open(IN,"$command|");
$fid = "frame-" . $i;
@@ -107,7 +146,18 @@ for($n=0; $n<$seqs->{nseq}; ++$n) {
$p=0;
while(<IN>) {
- if(!/sequences/) {
+ if ( $i == 28 ) { # print the raw file if window is 28
+ print RAW $_;
+ }
+ if ( /^\#/ ) {
+ chomp($result = $_);
+ $result =~ s/.*aas//;
+ $result =~ s/in coil\s*//g;
+ $result = "window size = $i ".$result." residues in coiled coil".
+ " domain\n";
+ push @result, $result;
+ next;
+ } else {
$_ =~ s/^ *//;
@t=split(/ +/);
$align->{ids}{$fid}{seq} .= $t[2];
@@ -118,18 +168,22 @@ for($n=0; $n<$seqs->{nseq}; ++$n) {
else { $sP = substr($P,0,1); }
if($sP eq "0") { $sP = "-"; }
$align->{ids}{$pid}{seq} .= $sP;
- } else {
- print;
}
}
close(IN);
}
- write_clustal($align,"-");
- }
-}
-#unlink $seqfile;
+
+
+ if ( defined($outfile)) { # Write output to file
+ write_clustal($align,\@result, $outfile);
+ } else {
+ write_clustal($align,\@result, "-");
+ }
+ }
+}
+unlink $seqfile;
-exit;
+exit(0);
sub write_fasta {
my($fn,$id,$seq) = @_;
@@ -139,7 +193,7 @@ sub write_fasta {
while($i<length($seq)) {
$aa = substr($seq,$i,1);
print SEQ $aa;
- if((($i+1)%60)==0){ print SEQ "\n" }
+ if((($i+1)%50)==0){ print SEQ "\n" }
$i++;
}
print SEQ "\n";
@@ -150,12 +204,14 @@ sub write_clustal {
my($align) = $_[0];
my($i,$j,$k,$id);
- my($outfile) = $_[1];
+ my($header)= $_[1];
+ my($outfile) = $_[2];
open(OUT,">$outfile") || die "Error opening output file $outfile\n";
-
-
# print OUT "CLUSTAL W(1.60) multiple sequence alignment\n\n";
+
+ print OUT @$header;
+ print OUT "\n";
foreach $id (keys %{$align->{ids}}) {
if(defined($align->{ids}{$id}{start})) {
@@ -164,24 +220,44 @@ sub write_clustal {
$align->{ids}{$id}{newid} .= $align->{ids}{$id}{ranges};
}
} else {
- $align->{ids}{$id}{newid} = $id;
- }
- }
- $i=0;
- while($i<$align->{alen}) {
- for($k=0; $k<$align->{nseq}; ++$k) {
- $id = $align->{list}[$k];
- printf(OUT "%-10s ",$align->{ids}{$id}{newid});
- for($j=0; $j<60; ++$j) {
- last if(($i+$j)>=$align->{alen});
- print OUT substr($align->{ids}{$id}{seq},($i+$j),1);
- }
- printf(OUT "\n");
- }
- $i+=60;
- printf(OUT "\n");
+ if ( $id !~ /frame/ and $id !~ /prob/) {
+ $align->{ids}{$id}{newid} = "seq";
+ } else {
+ $align->{ids}{$id}{newid} = $id;
+ }
+ } #
+ }
+ #
+ $i=0;
+
+ while($i<$align->{alen}) {
+ printf OUT "%-10s ", ' ';
+ for ($z = 1; $z <=50; $z++) {
+ if ($z == 50) {
+ print OUT ($i+50)/10 ;
+ } elsif ($z % 10 == 0) {
+ print OUT ":";
+ } elsif ($z % 5 == 0) {
+ print OUT ".";
+ } else {
+ print OUT " ";
+ }
+ }
+ print OUT "\n";
+
+ for($k=0; $k<$align->{nseq}; ++$k) {
+ $id = $align->{list}[$k];
+ printf(OUT "%-10s ",$align->{ids}{$id}{newid});
+ for($j=0; $j<50; ++$j) {
+ last if(($i+$j)>=$align->{alen});
+ print OUT substr($align->{ids}{$id}{seq},($i+$j),1);
+ }
+ print OUT "\n";
+ }
+ $i+=50;
}
- close(OUT);
+ print OUT "// End\n\n";
+ close OUT;
}
sub get_afasta {
@@ -222,7 +298,7 @@ sub get_afasta {
if(defined($align->{ids}{$label})) {
$_ =~ s/-/ /g;
$align->{ids}{$label}{seq} .= $_;
- print "Adding $_ to $label\n";
+# print "Adding $_ to $label\n";
}
}
}
@@ -233,6 +309,7 @@ sub get_afasta {
}
sub read_align { # Just read in text and modify format if necessary
+ #( $fastafile, \$type )
#
# Likely format is determined by looking for signs of one format over another
@@ -255,7 +332,7 @@ sub read_align { # Just read in text and
my($file) = $_[0];
my(@data);
my($i,$type);
- my($winner,$highest);
+ my($winner,$highest) = ('x', 0);
@data=();
@@ -268,18 +345,19 @@ sub read_align { # Just read in text and
for($i=0; $i<=$#data; ++$i) {
$_ = $data[$i];
- if(($i==0) && ($_ =~ / *[0-9]+ +[0-9]+ */)) { $votes{"y"}+=1000; }
+ if( $file =~ /\.fa(sta)?$/oi ){ $votes{"f"}+=1000; last; }
+ elsif(($i==0) && ($_ =~ / *[0-9]+ +[0-9]+ */)) { $votes{"y"}+=1000; }
+ elsif(($i==0) && ($_ =~ /^>/o) && $data[1] && $data[1] =~ /^[a-z]/io ) { $votes{"f"}+=10; }
elsif(($_ =~ /^ *Name:/) || ($_ =~ /pileUp/) || ($_ =~ /MSF.*Check.*\.\./)) { $votes{"m"}++; last; }
elsif($_ =~ /^CLUSTAL/) { $votes{"c"}+=10; last; }
elsif($_ =~ /^>P1;/) { $votes{"p"}+=10; last; }
- elsif($_ =~ /HMMER/) { $votes{"h"}+=10; last; }
+ elsif($_ =~ /HMMER/o && $data[0] !~ /^>/o) { $votes{"h"}+=10; last; }
elsif(($_ =~ /^#=SQ/) || ($_ =~ /^#=RF/)) { $votes{"h"}++; }
elsif($_ =~ /^>/) { $votes{"f"}++; $votes{"b"}++; }
elsif($_ =~ /^ *\* iteration [0-9]*/) { $votes{"b"}++; $block_start++; }
elsif($_ =~ /^ *\*/) { $votes{"b"}++; $block_end++; }
elsif(($_ =~ /^ID /) || ($_ =~ /^CC /) || ($_ =~ /^AC /) || ($_ =~ /^SE /)) { $votes{"s"}++; }
elsif(($_ =~ /^HSSP .*HOMOLOGY DERIVED SECONDARY STRUCTURE OF PROTEINS/)) { $votes{"e"}+=1000; }
-
}
# Block and FASTA are quite hard to tell apart in a quick parse,
@@ -289,14 +367,12 @@ sub read_align { # Just read in text and
if($block_end==0) { $votes{"f"}++; }
}
- $winner = "x";
- $highest = 0;
foreach $type (keys %votes) {
# print $type," ", $votes{$type},"\n";
if($votes{$type}>$highest) { $winner = $type; $highest = $votes{$type}; }
}
-# print "File is apparently of type $winner\n";
- $_[1] = $winner;
+ if( $dbg ){ warn( "File is apparently of type $winner" ); }
+ ${$_[1]} = $winner;
return @data;
}
sub get_print_string {
@@ -311,3 +387,5 @@ sub get_print_string {
}
return $max_len;
}
+
+# vim:et:ai:
--- a/ncoils.c
+++ b/ncoils.c
@@ -1,4 +1,5 @@
#include <ncoils.h>
+#include <string.h>
/* Copyright (c) 2002 Robert B. Russell
* EMBL, Meyerhofstrasse 1, 69917 Heidelberg, Germany
@@ -22,16 +23,19 @@
/* Rob Russell's attempt to make a COILS program */
+#ifndef __PKGDATADIR__
+#define __PKGDATADIR__ ""
+#endif
-main(int argc, char *argv[]) {
+int main(int argc, char *argv[]) {
- int i,j,k,l;
+ int i,j;
int verb;
int window,pt;
int which,weighted;
int nseq;
int t,tc;
- int seqlen;
+ int seqlen = 0;
int mode;
int min_seg;
@@ -39,7 +43,8 @@ main(int argc, char *argv[]) {
char heptfile[HEPTFILELENGTH];
char *buff;
static char *env;
- char *seq,*title,*ident;
+ char *seq = NULL;
+ char *title,*ident;
float min_P;
@@ -55,10 +60,10 @@ main(int argc, char *argv[]) {
mode = 0; /* 0 = column mode, 1 = fasta, 2 = concise */
min_P = 0.5;
- if((env=getenv("COILSDIR"))==NULL) {
+ if((env=getenv("COILSDIR"))==NULL) env = __PKGDATADIR__;
+
+ if( env[0] == 0 ){ // empty string
fprintf(stderr,"error: environment variable COILSDIR must be set\n");
- fprintf(stderr," Assuming /usr/share/ncoils\n");
- env=strdup("/usr/share/ncoils"); // a little leak, tolerated as singleton
exit(-1);
}
@@ -67,19 +72,19 @@ main(int argc, char *argv[]) {
for(i=1; i<argc; ++i) {
- if(argv[i][0]!='-') exit_error();
+ if(argv[i][0]!='-') exit_error( env );
if(strcmp(&argv[i][1],"m")==0) {
- if((i+1)>=argc) exit_error();
+ if((i+1)>=argc) exit_error( env );
strncpy(&heptfile[0],argv[i+1],HEPTFILELENGTH-1);
i++;
} else if(strcmp(&argv[i][1],"win")==0) {
- if((i+1)>=argc) exit_error();
+ if((i+1)>=argc) exit_error( env );
sscanf(argv[i+1],"%d",&window);
i++;
} else if(strcmp(&argv[i][1],"c")==0) {
mode=2;
} else if(strcmp(&argv[i][1],"min_seg")==0) {
- if((i+1)>=argc) exit_error();
+ if((i+1)>=argc) exit_error( env );
sscanf(argv[i+1],"%d",&min_seg);
i++;
} else if(strcmp(&argv[i][1],"c")==0) {
@@ -87,18 +92,18 @@ main(int argc, char *argv[]) {
} else if((strcmp(&argv[i][1],"f")==0) || (strcmp(&argv[i][1],"fasta")==0)) {
mode=1;
} else if((strcmp(&argv[i][1],"min_P")==0)) {
- if((i+1)>=argc) exit_error();
+ if((i+1)>=argc) exit_error( env );
sscanf(argv[i+1],"%f",&min_P);
i++;
} else if(strcmp(&argv[i][1],"help")==0) {
- exit_error();
+ exit_error( env );
} else if(strcmp(&argv[i][1],"w")==0) {
weighted=1;
} else if(strcmp(&argv[i][1],"V")==0 || strcmp(&argv[i][1],"v")==0) {
verb=1;
} else {
fprintf(stderr," can't understand flag/field %s\n",argv[i]);
- exit_error();
+ exit_error( env );
}
}
@@ -175,14 +180,14 @@ main(int argc, char *argv[]) {
pred_coils(seq,ident,title,h,window,which,weighted,mode,min_P,&t,&tc,min_seg);
free(seq);
}
- fprintf(stderr,"%8d sequences %8d aas %8d in coil\n",nseq,t,tc);
+ if( verb ) fprintf(stderr,"%8d sequences %8d aas %8d in coil\n",nseq,t,tc);
free(title); free(ident);
exit(0);
}
-void exit_error() {
+void exit_error( const char* __coilsdir ) {
fprintf(stderr,"format: ncoils [options] < [sequence file]\n");
fprintf(stderr," -f or -fasta [fasta output - coils as 'x', like '-x' in seg]\n");
fprintf(stderr," -c [concise mode - which sequences have any coils (and how many)]\n");
@@ -191,7 +196,7 @@ void exit_error() {
fprintf(stderr," -win <int> [window size; DEFAULT = 21]\n");
fprintf(stderr," -w [weight heptad positions a&d the same as b,c,e,f,g]\n");
fprintf(stderr," -v [verbose/debug mode - print extra junk]\n");
- fprintf(stderr," -max_seq_len <int> [longest sequence tolerated; DEFAULT = 100 000]\n");
+ fprintf(stderr,"Effective COILSDIR: %s\n", __coilsdir );
fprintf(stderr,"\n");
fprintf(stderr,"NCOILS, Rob Russell and Andrei Lupas, 1999\n");
fprintf(stderr," based on Lupas, Van Dyck & Stock (1991) Science 252,1162-1164\n");
@@ -206,7 +211,6 @@ void pred_coils(char *seq,char *ident,ch
int i,j;
int len,pos,aa_pt;
- int pt;
int total_coil_segments;
int are_there_coils;
--- /dev/null
+++ b/coils-wrap.pod
@@ -0,0 +1,77 @@
+=head1 NAME
+
+coils-wrap - runs ncoils three times to give you a three-window output
+
+=head1 SYNOPSIS
+
+coils-wrap [OPTION]
+
+=head1 DESCRIPTION
+
+coils-wrap runs ncoils three times to give you a three-window output.
+
+ncoils is a program that compares a sequence to a database of known parallel two-stranded coiled-coils and derives a similarity score. By comparing this score to the distribution of scores in globular and coiled-coil proteins, the program then calculates the probability that the sequence will adopt a coiled-coil conformation.
+
+=head1 OPTIONS
+
+=over
+
+=item -i
+
+input file in FASTA format
+
+=item -o
+
+output file
+
+=item -r
+
+raw output file, default: `<output file>_raw'
+
+=item -m
+
+matrix type; set matrix (MTK or MTIDK)
+
+=item -w
+
+weight a&d = b,c,e,f&g (boolean)
+
+=back
+
+=head1 EXAMPLES
+
+C<coils-wrap -m MTIDK -i /usr/share/doc/ncoils/1srya.fa -o /tmp/coils.out -r /tmp/coils_raw.txt && less /tmp/coils.out /tmp/coils_raw.txt>
+
+C<ncoils -win 14 < /usr/share/doc/ncoils/1srya.fa>
+
+=head1 FILES
+
+=over
+
+=item F</usr/share/coiledcoils/*.mat>
+
+matrix data files
+
+=back
+
+=head1 AUTHOR
+
+R.B. Russell, A.N. Lupas, 1999
+
+=head1 COPYRIGHT AND LICENSE
+
+GPL
+
+Based on Lupas, Van Dyck & Stock (1991) Science 252,1162-1164
+
+=head1 SEE ALSO
+
+L<ncoils(1)>, L</usr/share/doc/coils/README>, L<http://www.russelllab.org/cgi-bin/coils/coils-svr.pl>
+
+=over
+
+=item A Lupas: Prediction and Analysis of Coiled-Coil Structures. Methods in Enzymology, 266, 513-525, 1996
+
+=item A. Lupas, M. Van Dyke, and J. Stock: Predicting coiled coils from protein sequences. Science, 252, 1162-1164, 1991
+
+=back
--- /dev/null
+++ b/ncoils.pod
@@ -0,0 +1,89 @@
+=head1 NAME
+
+ncoils - prediction of coiled-coil secondary structure elements
+
+=head1 SYNOPSIS
+
+ncoils [OPTION] < [FASTA FILE]
+
+ncoils -f < /usr/share/doc/ncoils/1srya.fa
+
+=head1 DESCRIPTION
+
+ncoils is a program that compares a sequence to a database of known parallel two-stranded coiled-coils and derives a similarity score. By comparing this score to the distribution of scores in globular and coiled-coil proteins, the program then calculates the probability that the sequence will adopt a coiled-coil conformation.
+
+=head1 OPTIONS
+
+=over
+
+=item -f, -fasta
+
+fasta output - coils as 'x', like '-x' in seg
+
+=item -c
+
+concise mode - which sequences have any coils (and how many)
+
+=item -min_seg <int>
+
+for concise mode - only report sequence if >= min coil segments
+
+=item -min_P <float>
+
+minimum P to define coil segment; DEFAULT = 0.5
+
+=item -win <int>
+
+window size; DEFAULT = 21
+
+=item -w
+
+weight heptad positions a&d the same as b,c,e,f,g
+
+=item -v
+
+verbose/debug mode - print extra junk
+
+=back
+
+=head1 EXAMPLES
+
+C<coils-wrap -m MTIDK -i /usr/share/doc/ncoils/1srya.fa -o /tmp/coils.out -r /tmp/coils_raw.txt && less /tmp/coils.out /tmp/coils_raw.txt>
+
+C<ncoils -win 14 < /usr/share/doc/ncoils/1srya.fa>
+
+=head1 ENVIRONMENT
+
+COILSDIR - specifies the directory containing the file new.mat overriding the default F</usr/share/ncoils>
+
+=head1 FILES
+
+=over
+
+=item F</usr/share/ncoils/*.mat>
+
+matrix data files
+
+=back
+
+=head1 AUTHOR
+
+R.B. Russell, A.N. Lupas
+
+=head1 COPYRIGHT AND LICENSE
+
+GPL
+
+Based on Lupas, Van Dyck & Stock (1991) Science 252,1162-1164
+
+=head1 SEE ALSO
+
+L<coils-wrap(1)>, L</usr/share/doc/coils/README>, L<http://www.russelllab.org/cgi-bin/coils/coils-svr.pl>
+
+=over
+
+=item A Lupas: Prediction and Analysis of Coiled-Coil Structures. Methods in Enzymology, 266, 513-525, 1996
+
+=item A. Lupas, M. Van Dyke, and J. Stock: Predicting coiled coils from protein sequences. Science, 252, 1162-1164, 1991
+
+=back
--- a/ncoils.h
+++ b/ncoils.h
@@ -1,7 +1,6 @@
#include <stdio.h>
#include <stdlib.h>
#include <math.h>
-#include <string.h>
#define AAs "A_CDEFGHI_KLMN_PQRST_VW_Y_"
#define PI 3.1415
--- a/read_matrix.c
+++ b/read_matrix.c
@@ -1,4 +1,5 @@
#include <ncoils.h>
+#include <string.h>
/* Copyright (c) 2002 Robert B. Russell
* EMBL, Meyerhofstrasse 1, 69917 Heidelberg, Germany
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