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#ifndef ASSEMBLY_BUBBLEALGORITHM_H
#define ASSEMBLY_BUBBLEALGORITHM_H 1
#include "Common/IOUtil.h"
#include "Common/Options.h" // for opt::rank
#include <fstream>
#include <sstream>
namespace AssemblyAlgorithms {
static inline bool
processBranchGroupExtension(BranchGroup& group,
size_t branchIndex,
const graph_traits<SequenceCollectionHash>::vertex_descriptor& seq,
SequenceCollectionHash::SymbolSetPair ext,
int multiplicity,
unsigned maxLength);
template <typename Graph>
void collapseJoinedBranches(Graph* collection,
BranchGroup& group);
static inline
void writeBubble(std::ostream& out, const BranchGroup& group, unsigned id);
/** Open the bubble file. */
static inline
void openBubbleFile(std::ofstream& out)
{
if (opt::snpPath.empty())
return;
std::string path;
if (opt::rank < 0) {
path = opt::snpPath;
} else {
std::ostringstream s;
s << "snp-" << opt::rank << ".fa";
path = s.str();
}
out.open(path.c_str());
assert_good(out, path);
}
/** Pop bubbles. */
static inline
size_t popBubbles(SequenceCollectionHash* seqCollection, std::ostream& out)
{
typedef SequenceCollectionHash Graph;
typedef graph_traits<Graph>::vertex_descriptor V;
typedef Graph::SymbolSetPair SymbolSetPair;
Timer timer("PopBubbles");
size_t numPopped = 0;
// Set the cutoffs
const unsigned maxNumBranches = 3;
const unsigned maxLength = opt::bubbleLen - V::length() + 1;
for (Graph::iterator iter = seqCollection->begin();
iter != seqCollection->end(); ++iter) {
if (iter->second.deleted())
continue;
SymbolSetPair extRec = iter->second.extension();
for (extDirection dir = SENSE; dir <= ANTISENSE; ++dir) {
if (extRec.dir[dir].isAmbiguous()) {
// Found a potential bubble, examine each branch
bool stop = false;
// Create the branch group
BranchGroup branchGroup(dir, maxNumBranches,
iter->first);
initiateBranchGroup(branchGroup, iter->first,
extRec.dir[dir]);
// Iterate over the branches
while(!stop)
{
size_t numBranches = branchGroup.size();
for (unsigned j = 0; j < numBranches; ++j) {
// Get the extensions of this branch
SymbolSetPair extRec;
int multiplicity = -1;
const V& lastKmer
= branchGroup[j].back().first;
bool success = seqCollection->getSeqData(
lastKmer, extRec, multiplicity);
assert(success);
(void)success;
processBranchGroupExtension(branchGroup, j,
lastKmer, extRec, multiplicity,
maxLength);
}
// At this point all branches should have the same
// length or one will be a noext.
branchGroup.updateStatus(maxLength);
BranchGroupStatus status
= branchGroup.getStatus();
if (status == BGS_TOOLONG
|| status == BGS_TOOMANYBRANCHES
|| status == BGS_NOEXT) {
stop = true;
}
else if(status == BGS_JOINED)
{
static unsigned snpID;
writeBubble(out, branchGroup, ++snpID);
assert(branchGroup.isAmbiguous(
*seqCollection));
collapseJoinedBranches(seqCollection,
branchGroup);
assert(!branchGroup.isAmbiguous(
*seqCollection));
numPopped++;
stop = true;
} else
assert(status == BGS_ACTIVE);
}
}
}
seqCollection->pumpNetwork();
}
if (numPopped > 0)
std::cout << "Removed " << numPopped << " bubbles.\n";
if (!opt::db.empty()) {
addToDb("totalErodedTips", tempCounter[0]);
addToDb("totalPrunedTips", tempCounter[1]);
addToDb("totalLowCovCntg", tempCounter[3]);
addToDb("totalLowCovKmer", tempCounter[4]);
addToDb("totalSplitAmbg", tempCounter[7]);
addToDb("poppedBubbles", numPopped);
tempCounter.assign(16,0);
}
return numPopped;
}
// Populate a branch group with the inital branches from a sequence
static inline void
initiateBranchGroup(BranchGroup& group,
const graph_traits<SequenceCollectionHash>::vertex_descriptor& seq,
const SequenceCollectionHash::SymbolSet& extension)
{
typedef SequenceCollectionHash Graph;
typedef graph_traits<Graph>::vertex_descriptor V;
std::vector<V> extSeqs;
generateSequencesFromExtension(seq, group.getDirection(),
extension, extSeqs);
assert(extSeqs.size() > 1);
for (std::vector<V>::iterator seqIter = extSeqs.begin();
seqIter != extSeqs.end(); ++seqIter)
group.addBranch(BranchRecord(group.getDirection()), *seqIter);
}
/** Process an a branch group extension. */
static inline bool
processBranchGroupExtension(BranchGroup& group,
size_t branchIndex,
const graph_traits<SequenceCollectionHash>::vertex_descriptor& seq,
SequenceCollectionHash::SymbolSetPair ext,
int multiplicity,
unsigned maxLength)
{
typedef SequenceCollectionHash Graph;
typedef graph_traits<Graph>::vertex_descriptor V;
typedef vertex_bundle_type<Graph>::type VP;
BranchRecord& branch = group[branchIndex];
branch.setData(std::make_pair(seq, VP(multiplicity, ext)));
extDirection dir = group.getDirection();
if (ext.dir[!dir].isAmbiguous()) {
// Check that this fork is due to branches of our bubble
// merging back together. If not, stop this bubble.
if (branch.size() < 2) {
group.setNoExtension();
return false;
}
std::vector<V> extKmer;
generateSequencesFromExtension(seq, !dir,
ext.dir[!dir], extKmer);
assert(extKmer.size() > 1);
for (std::vector<V>::iterator it = extKmer.begin();
it != extKmer.end(); ++it) {
assert(branch.size() > 1);
if (!group.exists(branch.size() - 2, *it)) {
group.setNoExtension();
return false;
}
}
// Ignore the ambiguity.
ext.dir[!dir].clear();
}
if (ext.dir[dir].isAmbiguous()) {
// Create a new branch to follow the fork.
std::vector<V> extKmer;
generateSequencesFromExtension(seq, dir,
ext.dir[dir], extKmer);
assert(extKmer.size() > 1);
BranchRecord original = branch;
std::vector<V>::iterator it = extKmer.begin();
branch.push_back(std::make_pair(*it++, VP()));
for (; it != extKmer.end(); ++it)
group.addBranch(original, *it);
return group.isExtendable();
}
V nextKmer = seq;
if (processLinearExtensionForBranch(branch,
nextKmer, ext, multiplicity,
maxLength, false))
branch.push_back(std::make_pair(nextKmer, VP()));
else
group.setNoExtension();
return group.isExtendable();
}
/** Write a bubble to the specified file. */
static inline
void writeBubble(std::ostream& out, const BranchGroup& group, unsigned id)
{
if (opt::snpPath.empty())
return;
char allele = 'A';
for (BranchGroup::const_iterator it = group.begin();
it != group.end(); ++it) {
const BranchRecord& currBranch = *it;
Sequence contig(currBranch);
out << '>' << id << allele++ << ' '
<< contig.length() << ' '
<< currBranch.calculateBranchMultiplicity() << '\n'
<< contig.c_str() << '\n';
}
assert_good(out, opt::snpPath);
}
/** Collapse a bubble to a single path. */
template <typename Graph>
void collapseJoinedBranches(Graph* collection,
BranchGroup& group)
{
typedef typename graph_traits<Graph>::vertex_descriptor V;
typedef typename vertex_bundle_type<Graph>::type VP;
typedef typename std::map<V, VP> Map;
const BranchRecord& best = group[0];
logger(5) << "Popping " << best.size() << ' '
<< best.front().first << '\n';
// Add the k-mer from the dead branches.
Map doomed;
for (BranchGroup::const_iterator branchIt = group.begin() + 1;
branchIt != group.end(); ++branchIt) {
const BranchRecord& branch = *branchIt;
for (BranchRecord::const_iterator it = branch.begin();
it != branch.end(); ++it)
doomed.insert(*it);
}
// Remove the k-mer that are in the good branch.
for (BranchRecord::const_iterator it = best.begin();
it != best.end(); ++it)
doomed.erase(it->first);
// Remove the dead k-mer from the assembly.
for (typename Map::const_iterator it = doomed.begin();
it != doomed.end(); ++it)
removeSequenceAndExtensions(collection, *it);
}
} // namespace AssemblyAlgorithms
#endif
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