1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249
|
#include "Aligner.h"
#include "Iterator.h"
#include "SAM.h"
#include "Sequence.h"
#include <algorithm>
#include <cassert>
#include <cstdlib>
#include <iterator>
#include <utility>
using namespace std;
namespace opt {
/** For a duplicate k-mer in the target
* ERROR: report an error and exit
* MULTIMAP: report all alignments
* IGNORE: do not report any alignments
*/
int multimap;
}
template <>
void Aligner<SeqPosHashMultiMap>::addReferenceSequence(
const Kmer& kmer, Position pos)
{
assert(opt::multimap == opt::MULTIMAP);
m_target.insert(make_pair(kmer, pos));
}
template <class SeqPosHashMap>
void Aligner<SeqPosHashMap>::addReferenceSequence(
const Kmer& kmer, Position pos)
{
assert(opt::multimap != opt::MULTIMAP);
Kmer rc_kmer = reverseComplement(kmer);
map_iterator findIt = m_target.find(rc_kmer);
if (findIt != m_target.end()) {
if (!findIt->second.isDuplicate())
findIt->second.setDuplicate(
contigIndexToID(findIt->second.contig),
contigIndexToID(pos.contig), kmer.str());
return;
}
pair<map_iterator, bool> inserted
= m_target.insert(make_pair(kmer, pos));
if (!inserted.second)
if (!inserted.first->second.isDuplicate())
inserted.first->second.setDuplicate(
contigIndexToID(inserted.first->second.contig),
contigIndexToID(pos.contig), kmer.str());
}
/** Create an index of the target sequence. */
template <class SeqPosHashMap>
void Aligner<SeqPosHashMap>::addReferenceSequence(
const StringID& idString, const Sequence& seq)
{
unsigned id = contigIDToIndex(idString);
int size = seq.length();
for(int i = 0; i < (size - m_hashSize + 1); ++i)
{
Sequence subseq(seq, i, m_hashSize);
if (subseq.find_first_not_of("ACGT0123") != string::npos)
continue;
addReferenceSequence(Kmer(subseq), Position(id, i));
}
}
template <class SeqPosHashMap>
template <class oiterator>
void Aligner<SeqPosHashMap>::alignRead(
const string& qid, const Sequence& seq,
oiterator dest)
{
coalesceAlignments(qid, seq,
getAlignmentsInternal(seq, false), dest);
Sequence seqrc = reverseComplement(seq);
coalesceAlignments(qid, seqrc,
getAlignmentsInternal(seqrc, true), dest);
}
/** Store all alignments for a given Kmer in the parameter aligns.
* @param[out] aligns Map of contig IDs to alignment vectors.
*/
template <class SeqPosHashMap>
void Aligner<SeqPosHashMap>::alignKmer(
AlignmentSet& aligns, const Sequence& seq,
bool isRC, bool good, int read_ind, int seqLen)
{
assert(read_ind >= 0);
Sequence kmer(seq, read_ind, m_hashSize);
if (!good && kmer.find_first_not_of("ACGT0123") != string::npos)
return;
pair<map_const_iterator, map_const_iterator> range
= m_target.equal_range(Kmer(kmer));
if (range.first != range.second
&& opt::multimap == opt::IGNORE
&& range.first->second.isDuplicate())
return;
for (map_const_iterator resultIter = range.first;
resultIter != range.second; ++resultIter) {
assert(opt::multimap != opt::IGNORE
|| !resultIter->second.isDuplicate());
int read_pos = !isRC ? read_ind
: Alignment::calculateReverseReadStart(
read_ind, seqLen, m_hashSize);
unsigned ctgIndex = resultIter->second.contig;
Alignment align(string(),
resultIter->second.pos, read_pos, m_hashSize,
seqLen, isRC);
aligns[ctgIndex].push_back(align);
}
}
template <class SeqPosHashMap>
typename Aligner<SeqPosHashMap>::AlignmentSet
Aligner<SeqPosHashMap>::getAlignmentsInternal(
const Sequence& seq, bool isRC)
{
// The results
AlignmentSet aligns;
bool good = seq.find_first_not_of("ACGT0123") == string::npos;
int seqLen = seq.length();
int last_kmer = seqLen - m_hashSize;
if (last_kmer < 0)
return aligns;
// Align the first kmer
alignKmer(aligns, seq, isRC, good, 0, seqLen);
if (last_kmer == 0)
return aligns;
// Align the last kmer
alignKmer(aligns, seq, isRC, good, last_kmer, seqLen);
// Short-cut logic ignoring the middle alignments if the first
// and last kmers overlap, and align to the same contig
if (good && seqLen <= 2 * m_hashSize && aligns.size() == 1) {
AlignmentSet::const_iterator ctgIter = aligns.begin();
const AlignmentVector& a = ctgIter->second;
if (ctgIter->second.size() == 2) {
int qstep = isRC
? a[0].read_start_pos - a[1].read_start_pos
: a[1].read_start_pos - a[0].read_start_pos;
assert(qstep >= 0);
// Verify this isn't a kmer aligning to two parts of a
// contig, and that the alignments are coalescable.
if (qstep == last_kmer &&
a[1].contig_start_pos
== a[0].contig_start_pos + qstep)
return aligns;
}
}
// Align middle kmers
for(int i = 1; i < last_kmer; ++i)
alignKmer(aligns, seq, isRC, good, i, seqLen);
return aligns;
}
static int compareQueryPos(const Alignment& a1, const Alignment& a2)
{
return a1.read_start_pos < a2.read_start_pos;
}
/** Coalesce the k-mer alignments into a read alignment. */
template <class SeqPosHashMap>
template <class oiterator>
void Aligner<SeqPosHashMap>::coalesceAlignments(
const string& qid, const string& seq,
const AlignmentSet& alignSet,
oiterator& dest)
{
typedef typename output_iterator_traits<oiterator>::value_type
value_type;
for (AlignmentSet::const_iterator ctgIter = alignSet.begin();
ctgIter != alignSet.end(); ++ctgIter) {
AlignmentVector alignVec = ctgIter->second;
assert(!alignVec.empty());
sort(alignVec.begin(), alignVec.end(), compareQueryPos);
AlignmentVector::iterator prevIter = alignVec.begin();
AlignmentVector::iterator currIter = alignVec.begin() + 1;
Alignment currAlign = *prevIter;
while (currIter != alignVec.end()) {
int qstep = currIter->read_start_pos -
prevIter->read_start_pos;
assert(qstep >= 0);
int tstep = currIter->isRC ? -qstep : qstep;
if (currIter->contig_start_pos
== prevIter->contig_start_pos + tstep
&& qstep <= m_hashSize) {
currAlign.align_length += qstep;
if (currAlign.isRC)
currAlign.contig_start_pos -= qstep;
} else {
currAlign.contig = contigIndexToID(ctgIter->first);
*dest++ = value_type(currAlign, qid, seq);
currAlign = *currIter;
}
prevIter = currIter;
currIter++;
}
currAlign.contig = contigIndexToID(ctgIter->first);
*dest++ = value_type(currAlign, qid, seq);
}
}
// Explicit instantiation.
template void Aligner<SeqPosHashMultiMap>::addReferenceSequence(
const StringID& id, const Sequence& seq);
template void Aligner<SeqPosHashUniqueMap>::addReferenceSequence(
const StringID& id, const Sequence& seq);
template void Aligner<SeqPosHashMultiMap>::
alignRead<affix_ostream_iterator<Alignment> >(
const string& qid, const Sequence& seq,
affix_ostream_iterator<Alignment> dest);
template void Aligner<SeqPosHashUniqueMap>::
alignRead<affix_ostream_iterator<Alignment> >(
const string& qid, const Sequence& seq,
affix_ostream_iterator<Alignment> dest);
template void Aligner<SeqPosHashMultiMap>::
alignRead<ostream_iterator<SAMRecord> >(
const string& qid, const Sequence& seq,
ostream_iterator<SAMRecord> dest);
template void Aligner<SeqPosHashUniqueMap>::
alignRead<ostream_iterator<SAMRecord> >(
const string& qid, const Sequence& seq,
ostream_iterator<SAMRecord> dest);
|