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/**
* Resolve unitig repeats using a sliding window and
* and short read information.
* Written by Vladimir Nikolic <vnikolic@bcgsc.ca>
*/
#include "RAlgorithmsShort.h"
#if _OPENMP
#include <omp.h>
#endif
#include <fstream>
#include <getopt.h>
#include <iostream>
#include <sstream>
#include <string>
#define PROGRAM "abyss-rresolver-short"
static const char VERSION_MESSAGE[] =
PROGRAM " (" PACKAGE_NAME ") " VERSION "\n"
"Written by Vladimir Nikolic.\n"
"\n"
"Copyright 2020 Canada's Michael Smith Genome Sciences Centre\n";
static const char USAGE_MESSAGE[] =
"Usage: " PROGRAM " [OPTION]... <contigs> <graph> [<reads1> <reads2> ...]\n"
"Resolve unitig repeats using a sliding window and\n"
"and short read information.\n"
"\n"
" Arguments:\n"
"\n"
" <contigs> contigs in FASTA format\n"
" <graph> contig adjacency graph\n"
" <reads> reads in FASTA format\n"
"\n"
" Options:\n"
"\n"
" -b, --bloom-size=N read Bloom filter size. Unit suffixes 'K' (kilobytes), 'M' (megabytes), or 'G' (gigabytes) may be used. [required]\n"
" -g, --graph=FILE write the contig adjacency graph to FILE. [required]\n"
" -c, --contigs=FILE write the contigs to FILE. [required]\n"
" -j, --threads=N use N parallel threads [1]\n"
" -k, --kmer=N assembly k-mer size\n"
" -h, --hist=PREFIX write the algorithm histograms with the given prefix. Histograms are omitted if no prefix is given.\n"
" -t, --threshold=N set path support threshold to N. [4]\n"
" -x, --extract=N extract N r-mers per read. [4]\n"
" -m, --min-tests=N set minimum number of sliding window moves to N. Cannot be higher than 127. [18]\n"
" -M, --max-tests=N set maximum number of sliding window moves to N. Cannot be higher than 127. [40]\n"
" -n, --branching=N set maximum number of branching paths to N. [75]\n"
" -r, --rmer=N explicitly set r value (k value used by rresolver). The number of set r values should be equal to the number of read sizes.\n"
" -a, --approx-factor explicitly set coverage approximation factor.\n"
" -q, --quality--threshold=N minimum quality all bases in rmers should have, on average. [35] (UNUSED)\n"
" -e, --error-correction enable correction of a 1bp error in kmers. [false]\n"
" -R, --max-read-size upper limit on read size to consider for use with RResolver. [350]\n"
" -f, --bf-mem-factor factor to multiply Bloom filter memory budget with in order to stay within similar memory usage as the rest of the pipeline. [1.0]\n"
" -S, --supported=FILE write supported paths to FILE.\n"
" -U, --unsupported=FILE write unsupported paths to FILE.\n"
" Used for path sequence quality check.\n"
" --adj output the graph in ADJ format [default]\n"
" --asqg output the graph in ASQG format\n"
" --dot output the graph in GraphViz format\n"
" --gfa output the graph in GFA1 format\n"
" --gfa1 output the graph in GFA1 format\n"
" --gfa2 output the graph in GFA2 format\n"
" --gv output the graph in GraphViz format\n"
" --sam output the graph in SAM format\n"
" -v, --verbose display verbose output\n"
" --help display this help and exit\n"
" --version output version information and exit\n"
"\n"
"Report bugs to <" PACKAGE_BUGREPORT ">.\n";
namespace opt {
/** Read Bloom filter size in bytes. */
size_t bloomSize = 0;
/** The number of parallel threads */
int threads = 1;
/** Prefix for the histogram files */
std::string histPrefix;
/** Name of the file to write resulting graph to */
std::string outputGraphPath;
/** Name of the file to write resulting graph to */
std::string outputContigsPath;
/** Number of kmers required to be found for a path to be supported */
int threshold = 4;
/** Number of Rmers to extract per read */
int extract = 4;
/** Minimum number of sliding window moves */
int minTests = 18;
/** Maximum number of sliding window moves */
int maxTests = 40;
/** Maximum number of branching paths */
int branching = 75;
/** Explicitly specified r values. */
std::vector<int> rValues;
/** Explicitly set coverage approximation factors. */
std::vector<double> covApproxFactors;
/** Base pair quality threshold that large k-mers bases should have at minimum. */
int readQualityThreshold = 35;
/** Flag indicating whether error correction is enabled */
int errorCorrection = 0;
/** Upper limit on read size to consider for use with RResolver. */
unsigned maxReadSize = 350;
/** factor to multiply Bloom filter memory budget with in order to stay within similar memory usage as the rest of the pipeline. */
double bfMemFactor = 1.0;
/** Name of the file to write supported paths to */
std::string outputSupportedPathsPath;
/** Name of the file to write unsupported paths to */
std::string outputUnsupportedPathsPath;
unsigned k = 0; // used by ContigProperties
int format; // used by ContigProperties
}
static const char shortopts[] = "b:j:g:c:k:h:t:x:m:M:n:r:a:q:eR:f:S:U:v";
enum
{
OPT_HELP = 1,
OPT_VERSION
};
static const struct option longopts[] = {
{ "bloom-size", required_argument, NULL, 'b' },
{ "threads", required_argument, NULL, 'j' },
{ "graph", required_argument, NULL, 'g' },
{ "contigs", required_argument, NULL, 'c' },
{ "kmer", required_argument, NULL, 'k' },
{ "hist", required_argument, NULL, 'h' },
{ "threshold", required_argument, NULL, 't' },
{ "extract", required_argument, NULL, 'x' },
{ "min-tests", required_argument, NULL, 'm' },
{ "max-tests", required_argument, NULL, 'M' },
{ "branching", required_argument, NULL, 'n' },
{ "rmer", required_argument, NULL, 'r' },
{ "approx-factor", required_argument, NULL, 'a' },
{ "quality-threshold", required_argument, NULL, 'q' },
{ "error-correction", no_argument, &opt::errorCorrection, 1 },
{ "max-read-size", required_argument, NULL, 'R' },
{ "bf-mem-factor", required_argument, NULL, 'f' },
{ "supported", required_argument, NULL, 'S' },
{ "unsupported", required_argument, NULL, 'U' },
{ "adj", no_argument, &opt::format, ADJ },
{ "asqg", no_argument, &opt::format, ASQG },
{ "dot", no_argument, &opt::format, DOT },
{ "gfa", no_argument, &opt::format, GFA1 },
{ "gfa1", no_argument, &opt::format, GFA1 },
{ "gfa2", no_argument, &opt::format, GFA2 },
{ "gv", no_argument, &opt::format, DOT },
{ "sam", no_argument, &opt::format, SAM },
{ "verbose", no_argument, NULL, 'v' },
{ "help", no_argument, NULL, OPT_HELP },
{ "version", no_argument, NULL, OPT_VERSION },
{ NULL, 0, NULL, 0 }
};
void
check_options(int argc, bool& die)
{
if (opt::bloomSize == 0) {
std::cerr << PROGRAM ": missing or invalid value for mandatory option `-b'" << std::endl;
die = true;
}
if (argc - optind < 3) {
std::cerr << PROGRAM ": missing input file arguments" << std::endl;
die = true;
}
if (opt::k <= 0) {
std::cerr << PROGRAM ": missing or invalid value for mandatory option `-k'" << std::endl;
die = true;
}
if (opt::readQualityThreshold <= 0) {
std::cerr << PROGRAM ": invalid value for option `-q'" << std::endl;
die = true;
}
if (opt::outputGraphPath.empty()) {
std::cerr << PROGRAM ": missing or invalid value for mandatory option `-g`" << std::endl;
die = true;
}
if (opt::outputContigsPath.empty()) {
std::cerr << PROGRAM ": missing or invalid value for mandatory option `-c`" << std::endl;
die = true;
}
if (opt::threads <= 0) {
std::cerr << PROGRAM ": invalid number of threads `-j`" << std::endl;
die = true;
}
if (opt::minTests > opt::maxTests) {
std::cerr << PROGRAM ": --min-tests cannot be higher than --max-tests" << std::endl;
die = true;
}
}
void
writePaths(std::ostream& stream, const ImaginaryContigPaths& paths)
{
int counter = 0;
for (const auto& path : paths) {
stream << '>' << counter++ << '\n' << getPathSequence(path) << '\n';
}
}
void
writeResults(
const ImaginaryContigPaths& supportedPaths,
const ImaginaryContigPaths& unsupportedPaths,
const std::string& commandLine)
{
if (!opt::outputContigsPath.empty()) {
storeContigs(opt::outputContigsPath);
}
if (!opt::outputGraphPath.empty()) {
storeContigGraph(opt::outputGraphPath, PROGRAM, commandLine);
}
if (!opt::outputSupportedPathsPath.empty()) {
if (opt::verbose) {
std::cerr << "Writing supported paths to `" << opt::outputSupportedPathsPath << "'..."
<< std::endl;
}
std::ofstream outputSupportedPaths(opt::outputSupportedPathsPath);
writePaths(outputSupportedPaths, supportedPaths);
assert(outputSupportedPaths.good());
outputSupportedPaths.close();
if (opt::verbose) {
std::cerr << "Supported paths written." << std::endl;
}
}
if (!opt::outputUnsupportedPathsPath.empty()) {
if (opt::verbose) {
std::cerr << "Writing unsupported paths to `" << opt::outputUnsupportedPathsPath
<< "'..." << std::endl;
}
std::ofstream outputUnsupportedPaths(opt::outputUnsupportedPathsPath);
writePaths(outputUnsupportedPaths, unsupportedPaths);
assert(outputUnsupportedPaths.good());
outputUnsupportedPaths.close();
if (opt::verbose) {
std::cerr << "Unsupported paths written." << std::endl;
}
}
}
int
main(int argc, char** argv)
{
std::string commandLine;
{
std::ostringstream ss;
char** last = argv + argc - 1;
std::copy(argv, last, std::ostream_iterator<const char*>(ss, " "));
ss << *last;
commandLine = ss.str();
}
bool die = false; // scary, and spooky
for (int c; (c = getopt_long(argc, argv, shortopts, longopts, NULL)) != -1;) {
std::istringstream arg(optarg != NULL ? optarg : "");
switch (c) {
case '?':
die = true;
break;
case 'b':
opt::bloomSize = SIToBytes(arg);
break;
case 'j':
arg >> opt::threads;
break;
case 'k':
arg >> opt::k;
break;
case 'h':
arg >> opt::histPrefix;
break;
case 'g':
arg >> opt::outputGraphPath;
break;
case 'c':
arg >> opt::outputContigsPath;
break;
case 't':
arg >> opt::threshold;
break;
case 'x':
arg >> opt::extract;
break;
case 'm':
arg >> opt::minTests;
break;
case 'M':
arg >> opt::maxTests;
break;
case 'n':
arg >> opt::branching;
break;
case 'r':
int r;
arg >> r;
opt::rValues.push_back(r);
break;
case 'R':
arg >> opt::maxReadSize;
break;
case 'f':
arg >> opt::bfMemFactor;
break;
case 'a':
double a;
arg >> a;
opt::covApproxFactors.push_back(a);
break;
case 'q':
arg >> opt::readQualityThreshold;
break;
case 'S':
arg >> opt::outputSupportedPathsPath;
break;
case 'U':
arg >> opt::outputUnsupportedPathsPath;
break;
case 'v':
++opt::verbose;
break;
case OPT_HELP:
std::cout << USAGE_MESSAGE;
exit(EXIT_SUCCESS);
case OPT_VERSION:
std::cout << VERSION_MESSAGE;
exit(EXIT_SUCCESS);
}
if (optarg != NULL && (!arg.eof() || arg.fail())) {
std::cerr << PROGRAM ": invalid option: `-" << char(c) << optarg << "'" << std::endl;
exit(EXIT_FAILURE);
}
}
check_options(argc, die);
if (die) {
std::cerr << "Try `" << PROGRAM << " --help' for more information." << std::endl;
exit(EXIT_FAILURE);
}
#if _OPENMP
if (opt::threads > 0)
omp_set_num_threads(opt::threads);
#endif
std::string contigsPath(argv[optind++]);
std::string contigGraphPath(argv[optind++]);
loadContigGraph(contigGraphPath);
loadContigs(contigsPath);
std::vector<std::string> readFilepaths;
for (int i = optind; i < argc; i++) {
readFilepaths.push_back(argv[i]);
}
ImaginaryContigPaths supportedPaths, unsupportedPaths;
resolveShort(readFilepaths, supportedPaths, unsupportedPaths);
if (opt::verbose) {
std::cerr << "Stats after resolution:" << std::endl;
printGraphStats(std::cerr, g_contigGraph);
}
writeResults(supportedPaths, unsupportedPaths, commandLine);
return EXIT_SUCCESS;
}
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