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#include "config.h"
#include "Common/ContigPath.h"
#include "Common/IOUtil.h"
#include "Graph/ContigGraph.h"
#include "Graph/ContigGraphAlgorithms.h"
#include "Graph/DirectedGraph.h"
#include "Graph/GraphIO.h"
#include "Graph/GraphUtil.h"
#include "Uncompress.h"
#include <boost/graph/graph_traits.hpp>
#include <boost/lambda/bind.hpp>
#include <boost/lambda/lambda.hpp>
#include <boost/ref.hpp>
#include <algorithm>
#include <cassert>
#include <getopt.h>
#include <iostream>
#include <iterator>
#include <sstream>
#include <string>
#include <vector>
#include <utility>
using namespace std;
using namespace boost::lambda;
using boost::tie;
#define PROGRAM "abyss-junction"
static const char VERSION_MESSAGE[] =
PROGRAM " (" PACKAGE_NAME ") " VERSION "\n"
"Written by Shaun Jackman.\n"
"\n"
"Copyright 2014 Canada's Michael Smith Genome Sciences Centre\n";
static const char USAGE_MESSAGE[] =
"Usage: " PROGRAM " [OPTION]... OVERLAP [SCAFFOLD]...\n"
"Extend junction contigs that are supported by the scaffold graph.\n"
"\n"
" Arguments:\n"
"\n"
" OVERLAP the overlap graph\n"
" SCAFFOLD a scaffold graph\n"
"\n"
" Options:\n"
"\n"
" -i, --ignore=FILE ignore junctions seen in FILE\n"
" -v, --verbose display verbose output\n"
" --help display this help and exit\n"
" --version output version information and exit\n"
"\n"
"Report bugs to <" PACKAGE_BUGREPORT ">.\n";
namespace opt {
unsigned k; // used by DotIO
/** Do not check for evidence in the scaffold graph. */
bool noScaffoldGraph;
/** Junction contigs to ignore. */
string ignorePath;
/** Verbose output. */
int verbose; // used by PopBubbles
/** Output format */
int format = DOT; // used by DistanceEst
}
static const char shortopts[] = "i:v";
enum { OPT_HELP = 1, OPT_VERSION, };
static const struct option longopts[] = {
{ "ignore", no_argument, NULL, 'i' },
{ "verbose", no_argument, NULL, 'v' },
{ "help", no_argument, NULL, OPT_HELP },
{ "version", no_argument, NULL, OPT_VERSION },
{ NULL, 0, NULL, 0 }
};
/** Counts. */
static struct {
unsigned junctions;
unsigned supported;
} g_count;
/** An overlap graph. */
typedef DirectedGraph<NoProperty, NoProperty> DG;
typedef ContigGraph<DG> Graph;
typedef Graph OverlapGraph;
/** A scaffold graph. */
typedef Graph ScaffoldGraph;
/** Extend junction contigs. */
void extendJunction(
const OverlapGraph& overlapG,
const ScaffoldGraph& scaffoldG,
graph_traits<OverlapGraph>::vertex_descriptor v)
{
if (get(vertex_sense, overlapG, v)
|| in_degree(v, overlapG) != 1
|| out_degree(v, overlapG) != 1)
return;
typedef graph_traits<OverlapGraph>::vertex_descriptor V;
V u = source(*in_edges(v, overlapG).first, overlapG);
V w = *adjacent_vertices(v, overlapG).first;
if (opt::noScaffoldGraph
|| edge(u, w, scaffoldG).second) {
// This junction contig is supported by the scaffold graph.
ContigPath path;
path.reserve(3);
extend(overlapG, get(vertex_complement, overlapG, v),
back_inserter(path));
reverseComplement(path.begin(), path.end());
path.push_back(v);
extend(overlapG, v, back_inserter(path));
assert(path.size() >= 3);
cout << createContigName() << '\t' << path << '\n';
g_count.supported++;
}
g_count.junctions++;
}
/** Allow parallel edges. */
struct AllowParallelEdges {
template <typename EP>
EP operator()(const EP& a, const EP&) const
{
return a;
}
};
/** Read a graph from the specified file. */
static void readGraph(const string& path, Graph& g)
{
if (opt::verbose > 0)
cerr << "Reading `" << path << "'...\n";
ifstream fin(path.c_str());
istream& in = path == "-" ? cin : fin;
assert_good(in, path);
read_graph(in, g, AllowParallelEdges());
assert(in.eof());
if (opt::verbose > 1)
printGraphStats(cerr, g);
g_contigNames.lock();
}
int main(int argc, char** argv)
{
bool die = false;
for (int c; (c = getopt_long(argc, argv,
shortopts, longopts, NULL)) != -1;) {
istringstream arg(optarg != NULL ? optarg : "");
switch (c) {
case '?': die = true; break;
case 'i': arg >> opt::ignorePath; break;
case 'v': opt::verbose++; break;
case OPT_HELP:
cout << USAGE_MESSAGE;
exit(EXIT_SUCCESS);
case OPT_VERSION:
cout << VERSION_MESSAGE;
exit(EXIT_SUCCESS);
}
if (optarg != NULL && !arg.eof()) {
cerr << PROGRAM ": invalid option: `-"
<< (char)c << optarg << "'\n";
exit(EXIT_FAILURE);
}
}
if (argc - optind < 1) {
cerr << PROGRAM ": missing arguments\n";
die = true;
}
if (die) {
cerr << "Try `" << PROGRAM
<< " --help' for more information.\n";
exit(EXIT_FAILURE);
}
OverlapGraph overlapG;
readGraph(argv[optind++], overlapG);
ScaffoldGraph scaffoldG(overlapG.num_vertices() / 2);
if (optind < argc) {
for_each(argv + optind, argv + argc,
boost::lambda::bind(readGraph, _1, boost::ref(scaffoldG)));
// Add any missing complementary edges.
size_t numAdded = addComplementaryEdges(scaffoldG);
if (opt::verbose > 0)
cerr << "Added " << numAdded << " complementary edges.\n";
if (opt::verbose > 1)
printGraphStats(cerr, scaffoldG);
} else
opt::noScaffoldGraph = true;
// Read the set of contigs to ignore.
vector<bool> seen(num_vertices(overlapG) / 2);
if (!opt::ignorePath.empty()) {
ifstream in(opt::ignorePath.c_str());
assert_good(in, opt::ignorePath);
markSeenInPath(in, seen);
}
// Extend the junction contigs.
graph_traits<OverlapGraph>::vertex_iterator uit, ulast;
for (tie(uit, ulast) = vertices(overlapG); uit != ulast; ++uit)
if (!seen[get(vertex_contig_index, overlapG, *uit)])
extendJunction(overlapG, scaffoldG, *uit);
assert_good(cout, "stdout");
if (opt::verbose > 0) {
cerr << "Junctions: " << g_count.junctions << '\n';
if (!opt::noScaffoldGraph)
cerr << "Supported: " << g_count.supported << '\n';
}
return 0;
}
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