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#!/bin/bash
VERSION="0.0"
# Check dependencies
PROGRAM_DEPENDENCIES=( 'ImageMath' 'SmoothImage' 'ThresholdImage' 'antsRegistration' 'antsApplyTransforms' 'N4BiasFieldCorrection' 'Atropos' 'KellyKapowski' )
SCRIPTS_DEPENDENCIES=( 'antsCorticalThickness.sh' 'antsBrainExtraction.sh' 'antsAtroposN4.sh' 'antsJointLabelFusion.sh' )
for D in ${PROGRAM_DEPENDENCIES[@]};
do
if ! command -v ${D} &> /dev/null
then
echo "Error: we can't find the $D program."
echo "Perhaps you need to \(re\)define \$PATH in your environment."
exit
fi
done
for D in ${SCRIPT_DEPENDENCIES[@]};
do
if ! command -v ${D} &> /dev/null
then
echo "We can't find the $D script."
echo "Perhaps you need to \(re\)define \$PATH in your environment."
exit
fi
done
function Usage {
cat <<USAGE
`basename $0` initializes tissue priors for a brain template. The following steps
are performed:
1. Run the template through antsCorticalThickness
2. Create priors for the template
a. The brain extraction prior is created by smoothing the brain extraction
mask created during 1.
b. If labeled atlases are not provided, we smooth the posteriors from 2a to create
the new segmentation priors, otherwise we use antsJointLabelFusion to create a set of
posteriors (https://github.com/ntustison/antsCookTemplatePriorsExample).
Usage:
`basename $0` -d imageDimension
-e brainTemplate
-m brainExtractionProbabilityMask
-p brainSegmentationPriors
<OPTARGS>
-o outputPrefix
\${templateImages[@]}
Example:
bash $0 -d 3 -e brainWithSkullTemplate.nii.gz -m brainPrior.nii.gz -p segmentationPriors%d.nii.gz -o output \${templateImages[@]}
Required arguments:
-d: Image dimension 2 or 3 (for 2- or 3-dimensional image)
-e: Brain reference template Anatomical *intensity* template (possibly created using a population
data set with buildtemplateparallel.sh in ANTs). This template is
*not* skull-stripped.
-m: Brain extraction probability mask Brain *probability* mask created using e.g. LPBA40 labels which
have brain masks defined, and warped to anatomical template and
averaged resulting in a probability image.
-p: Brain segmentation priors Tissue *probability* priors corresponding to the image specified
with the -e option. Specified using c-style formatting, e.g.
-p labelsPriors%02d.nii.gz. We assume that the first four priors
are ordered as follows
1: csf
2: cortical gm
3: wm
4: deep gm
-o: Output prefix The following subdirectory and images are created for the single
subject template
* \${OUTPUT_PREFIX}SingleSubjectTemplate/
* \${OUTPUT_PREFIX}SingleSubjectTemplate/T_template*.nii.gz
anatomical images Set of multimodal input data assumed to be specified ordered as
follows:
\${time1_modality1} \${time1_modality2} ... \${time1_modalityN} \\
\${time2_modality1} \${time2_modality2} ...
.
.
.
\${timeN_modality1} ...
A single modality is expected by default, in which case the input images
are simply ordered by time:
\${time1_modality1} \${time2_modality1} ... \${timeN_modality1}
If there are multiple modalities, use the -k option to specify how many.
Optional arguments:
-s: image file suffix Any of the standard ITK IO formats e.g. nrrd, nii.gz (default), mhd
-c: control type Control for parallel computation (default 0):
0 = run serially
1 = SGE qsub
2 = use PEXEC (localhost)
3 = Apple XGrid
4 = PBS qsub
5 = SLURM
-a: Atlases (assumed to be skull-stripped) used to cook template priors. If atlases
aren't used then we simply smooth the single-subject template posteriors after
passing through antsCorticalThickness.sh. Example:
-a atlas1.nii.gz -a atlas2.nii.gz ... -a atlasN.nii.gz
-l: Labels associated with each atlas, in the same order as they are specified
with the -a option. The number of labels in each image is assumed to be equal
to the number of priors.
-f: extraction registration mask Mask (defined in the template space) used during registration
for brain extraction.
-g: denoise anatomical images Denoise anatomical images (default = 0).
-j: number of cpu cores Number of cpu cores to use locally for pexec option (default 2; requires "-c 2")
-k: number of modalities Number of modalities used to construct the template (default 1): For example,
if one wanted to use multiple modalities consisting of T1, T2, and FA
components ("-k 3").
-u: use floating-point precision Use floating point precision in registrations (default = 0)
-v: Atropos segmentation weight Atropos spatial prior *probability* weight for the segmentation for the
template (default = 0.25)
-w: Atropos segmentation weight Atropos spatial prior *probability* weight for the segmentations (default = 0.5)
-q: Use quick registration parameters If 'yes' then we use antsRegistrationSyNQuick.sh as the basis for registration.
Otherwise use antsRegistrationSyN.sh. The options are as follows:
'-q 0' = antsRegistrationSyN for everything (default)
'-q 1' = Fast antsCorticalThickness to SST
'-q 2' = Fast JLF cooking
'-q 3' = Fast everything
-z: Test / debug mode If > 0, runs a faster version of the script. Only for testing. Implies -u 0
in the antsCorticalThickness.sh script (i.e., no random seeding).
Requires single thread computation for complete reproducibility.
USAGE
exit 1
}
echoParameters() {
cat <<PARAMETERS
Using antsLongitudinalCorticalThickness with the following arguments:
image dimension = ${DIMENSION}
anatomical image = ${ANATOMICAL_IMAGES[@]}
brain template = ${BRAIN_TEMPLATE}
extraction prior = ${EXTRACTION_PRIOR}
segmentation prior = ${SEGMENTATION_PRIOR}
output prefix = ${OUTPUT_PREFIX}
output image suffix = ${OUTPUT_SUFFIX}
registration template = ${REGISTRATION_TEMPLATE}
Other parameters:
run quick = ${RUN_QUICK}
debug mode = ${DEBUG_MODE}
float precision = ${USE_FLOAT_PRECISION}
denoise = ${DENOISE}
use random seeding = ${USE_RANDOM_SEEDING}
number of modalities = ${NUMBER_OF_MODALITIES}
number of cores = ${CORES}
control type = ${DOQSUB}
rigid alignment to SST = ${RIGID_ALIGNMENT_TO_SST}
PARAMETERS
}
# Echos a command to stdout, then runs it
# Will immediately exit on error unless you set debug flag here
DEBUG_MODE=0
function logCmd() {
cmd="$@"
echo "BEGIN >>>>>>>>>>>>>>>>>>>>"
echo $cmd
( "$@" )
cmdExit=$?
if [[ $cmdExit -gt 0 ]];
then
echo "ERROR: command exited with nonzero status $cmdExit"
echo "Command: $cmd"
echo
if [[ ! $DEBUG_MODE -gt 0 ]];
then
exit 1
fi
fi
echo "END <<<<<<<<<<<<<<<<<<<<"
echo
echo
return $cmdExit
}
HOSTNAME=`hostname`
DATE=`date`
CURRENT_DIR=`pwd`/
OUTPUT_DIR=${CURRENT_DIR}/tmp$RANDOM/
OUTPUT_PREFIX=${OUTPUT_DIR}/tmp
OUTPUT_SUFFIX="nii.gz"
DIMENSION=3
NUMBER_OF_MODALITIES=1
TEMPLATE_IMAGES=()
RUN_QUICK=1
USE_RANDOM_SEEDING=1
BRAIN_TEMPLATE=""
EXTRACTION_PRIOR=""
EXTRACTION_REGISTRATION_MASK=""
SEGMENTATION_PRIOR=""
USE_SST_CORTICAL_THICKNESS_PRIOR=0
REGISTRATION_TEMPLATE=""
DO_REGISTRATION_TO_TEMPLATE=0
DENOISE=0
ATROPOS_SEGMENTATION_PRIOR_WEIGHT_SST=0.25
ATROPOS_SEGMENTATION_PRIOR_WEIGHT_TIMEPOINT=0.5
DOQSUB=0
CORES=2
RIGID_ALIGNMENT_TO_SST=0
MALF_ATLASES=()
MALF_LABELS=()
MALF_LABEL_STRINGS_FOR_PRIORS=()
################################################################################
#
# Programs and their parameters
#
################################################################################
USE_FLOAT_PRECISION=0
if [[ $# -lt 3 ]] ; then
Usage >&2
exit 1
else
while getopts "a:b:c:d:e:f:g:h:j:k:l:m:o:p:q:r:s:t:u:v:w:z:" OPT
do
case $OPT in
a)
MALF_ATLASES[${#MALF_ATLASES[@]}]=$OPTARG
;;
b) # posterior formulation
ATROPOS_SEGMENTATION_POSTERIOR_FORMULATION=$OPTARG
;;
c)
DOQSUB=$OPTARG
if [[ $DOQSUB -gt 5 ]];
then
echo " DOQSUB must be an integer value (0=serial, 1=SGE qsub, 2=try pexec, 3=XGrid, 4=PBS qsub, 5=SLURM ) you passed -c $DOQSUB "
exit 1
fi
;;
d) #dimensions
DIMENSION=$OPTARG
if [[ ${DIMENSION} -gt 3 || ${DIMENSION} -lt 2 ]];
then
echo " Error: ImageDimension must be 2 or 3 "
exit 1
fi
;;
e) #brain extraction anatomical image
BRAIN_TEMPLATE=$OPTARG
;;
f) #brain extraction registration mask
EXTRACTION_REGISTRATION_MASK=$OPTARG
;;
g) #denoise
DENOISE=$OPTARG
;;
h) #help
Usage >&2
exit 0
;;
j) #number of cpu cores to use (default = 2)
CORES=$OPTARG
;;
k) #number of modalities
NUMBER_OF_MODALITIES=$OPTARG
;;
l)
MALF_LABELS[${#MALF_LABELS[@]}]=$OPTARG
;;
m) #brain extraction prior probability mask
EXTRACTION_PRIOR=$OPTARG
;;
n) # use
USE_SST_CORTICAL_THICKNESS_PRIOR=$OPTARG
;;
o) #output prefix
OUTPUT_PREFIX=$OPTARG
;;
p) #brain segmentation label prior image
SEGMENTATION_PRIOR=$OPTARG
;;
q) # run quick
RUN_QUICK=$OPTARG
;;
u) #use floating point precision
USE_FLOAT_PRECISION=$OPTARG
;;
v) #atropos prior weight for single subject template
ATROPOS_SEGMENTATION_PRIOR_WEIGHT_SST=$OPTARG
;;
w) #atropos prior weight for each individual time point
ATROPOS_SEGMENTATION_PRIOR_WEIGHT_TIMEPOINT=$OPTARG
;;
z) #debug mode
DEBUG_MODE=$OPTARG
;;
*) # getopts issues an error message
echo "ERROR: unrecognized option -$OPT $OPTARG"
exit 1
;;
esac
done
fi
FORMAT=${SEGMENTATION_PRIOR}
PREFORMAT=${FORMAT%%\%*}
POSTFORMAT=${FORMAT##*d}
FORMAT=${FORMAT#*\%}
FORMAT=${FORMAT%%d*}
REPCHARACTER=''
TOTAL_LENGTH=0
if [[ ${#FORMAT} -eq 2 ]]
then
REPCHARACTER=${FORMAT:0:1}
TOTAL_LENGTH=${FORMAT:1:1}
fi
# MAXNUMBER=$(( 10 ** $TOTAL_LENGTH ))
MAXNUMBER=1000
PRIOR_IMAGE_FILENAMES=()
WARPED_PRIOR_IMAGE_FILENAMES=()
BRAIN_SEGMENTATION_OUTPUT=${OUTPUT_PREFIX}BrainSegmentation
SEGMENTATION_WARP_OUTPUT_PREFIX=${BRAIN_SEGMENTATION_OUTPUT}Prior
SEGMENTATION_PRIOR_WARPED=${SEGMENTATION_WARP_OUTPUT_PREFIX}Warped
for (( i = 1; i < $MAXNUMBER; i++ ))
do
NUMBER_OF_REPS=$(( $TOTAL_LENGTH - ${#i} ))
ROOT='';
for(( j=0; j < $NUMBER_OF_REPS; j++ ))
do
ROOT=${ROOT}${REPCHARACTER}
done
FILENAME=${PREFORMAT}${ROOT}${i}${POSTFORMAT}
WARPED_FILENAME=${SEGMENTATION_PRIOR_WARPED}${ROOT}${i}.${OUTPUT_SUFFIX}
if [[ -f $FILENAME ]];
then
PRIOR_IMAGE_FILENAMES=( ${PRIOR_IMAGE_FILENAMES[@]} $FILENAME )
WARPED_PRIOR_IMAGE_FILENAMES=( ${WARPED_PRIOR_IMAGE_FILENAMES[@]} $WARPED_FILENAME )
else
break 1
fi
done
NUMBER_OF_PRIOR_IMAGES=${#WARPED_PRIOR_IMAGE_FILENAMES[*]}
# Shiftsize is calculated because a variable amount of arguments can be used on the command line.
# The shiftsize variable will give the correct number of arguments to skip. Issuing shift $shiftsize will
# result in skipping that number of arguments on the command line, so that only the input images remain.
shiftsize=$(($OPTIND - 1))
shift $shiftsize
# The invocation of $* will now read all remaining arguments into the variable IMAGESETVARIABLE
IMAGESETVARIABLE=$*
NINFILES=$(($nargs - $shiftsize))
IMAGESETARRAY=()
for IMG in $IMAGESETVARIABLE
do
TEMPLATE_IMAGES[${#TEMPLATE_IMAGES[@]}]=$IMG
done
if [[ ${#TEMPLATE_IMAGES[@]} -eq 0 ]];
then
echo "Error: no template images specified."
exit 1
fi
if [[ $NUMBER_OF_MODALITIES -gt 1 ]];
then
echo "--------------------------------------------------------------------------------------"
echo " Cortical thickness using the following ${NUMBER_OF_MODALITIES}-tuples: "
echo "--------------------------------------------------------------------------------------"
for (( i = 0; i < ${#TEMPLATE_IMAGES[@]}; i+=$NUMBER_OF_MODALITIES ))
do
IMAGEMETRICSET=""
for (( j = 0; j < $TEMPLATE_IMAGES; j++ ))
do
k=0
let k=$i+$j
IMAGEMETRICSET="$IMAGEMETRICSET ${TEMPLATE_IMAGES[$k]}"
done
echo $IMAGEMETRICSET
done
echo "--------------------------------------------------------------------------------------"
fi
if [[ ${#MALF_ATLASES[@]} -ne ${#MALF_LABELS[@]} ]]
then
echo "Error: The number of malf atlases and labels aren't equal."
fi
# Set up various things related to RUN_QUICK
# Can't do everything fast and still get good results if there is large deformation.
# Initiate levels of fast:
# 0 - Fast SST (old ANTS) but everything else slower for quality
# 1 - + Fast antsct to SST
# 2 - + Fast MALF cooking
# 3 - + Fast everything
RUN_OLD_ANTS_SST_CREATION=1
RUN_ANTSCT_TO_SST_QUICK=0
RUN_FAST_MALF_COOKING=0
RUN_FAST_ANTSCT_TO_GROUP_TEMPLATE=0
if [[ $RUN_QUICK -gt 0 ]];
then
RUN_ANTSCT_TO_SST_QUICK=1
fi
if [[ $RUN_QUICK -gt 1 ]];
then
RUN_FAST_MALF_COOKING=1
fi
if [[ $RUN_QUICK -gt 2 ]];
then
RUN_FAST_ANTSCT_TO_GROUP_TEMPLATE=1
fi
################################################################################
#
# Preliminaries:
# 1. Check existence of inputs
# 2. Figure out output directory and mkdir if necessary
# 3. See if $REGISTRATION_TEMPLATE is the same as $BRAIN_TEMPLATE
#
################################################################################
for (( i = 0; i < ${#TEMPLATE_IMAGES[@]}; i++ ))
do
if [[ ! -f ${TEMPLATE_IMAGES[$i]} ]];
then
echo "The specified image \"${TEMPLATE_IMAGES[$i]}\" does not exist."
exit 1
fi
done
################################################################################
#
# Run template through antsCorticalThickness.sh
#
################################################################################
TEMPLATE_EXTRACTION_MASK=${OUTPUT_PREFIX}BrainExtractionMask.${OUTPUT_SUFFIX}
TEMPLATE_EXTRACTION_REGISTRATION_MASK=${OUTPUT_PREFIX}BrainExtractionRegistrationMask.${OUTPUT_SUFFIX}
TEMPLATE_PRIOR=${OUTPUT_PREFIX}Priors\%${FORMAT}d.${OUTPUT_SUFFIX}
TEMPLATE_EXTRACTION_PRIOR=${OUTPUT_PREFIX}BrainExtractionMaskPrior.${OUTPUT_SUFFIX}
TEMPLATE_CORTICAL_THICKNESS=${OUTPUT_PREFIX}CorticalThickness.${OUTPUT_SUFFIX}
TEMPLATE_SKULL_STRIPPED=${OUTPUT_PREFIX}BrainExtractionBrain.${OUTPUT_SUFFIX}
echo
echo "--------------------------------------------------------------------------------------"
echo " Creating template priors: running template through antsCorticalThickness "
echo "--------------------------------------------------------------------------------------"
echo
time_start_priors=`date +%s`
TEMPLATE_IMAGES_LIST=''
for (( j=0; j < $NUMBER_OF_MODALITIES; j++ ))
do
TEMPLATE_IMAGES_LIST="${TEMPLATE_IMAGES_LIST} -a ${TEMPLATE_IMAGES[$j]}"
done
REG_MASK=""
if [[ -f ${EXTRACTION_REGISTRATION_MASK} ]]; then
REG_MASK="-f ${EXTRACTION_REGISTRATION_MASK}"
fi
if [[ ! -f ${TEMPLATE_CORTICAL_THICKNESS} ]];
then
logCmd antsCorticalThickness.sh \
-d ${DIMENSION} \
-q ${RUN_FAST_ANTSCT_TO_GROUP_TEMPLATE} \
$TEMPLATE_IMAGES_LIST \
$REG_MASK \
-e ${BRAIN_TEMPLATE} \
-g ${DENOISE} \
-m ${EXTRACTION_PRIOR} \
-k 0 \
-z ${DEBUG_MODE} \
-p ${SEGMENTATION_PRIOR} \
-w ${ATROPOS_SEGMENTATION_PRIOR_WEIGHT_SST} \
-o ${OUTPUT_PREFIX}
fi
TEMPLATE_POSTERIORS=( ${OUTPUT_PREFIX}BrainSegmentationPosteriors*.${OUTPUT_SUFFIX} )
TEMPLATE_POSTERIORS_EXIST=1
TEMPLATE_PRIORS_EXIST=1
TEMPLATE_PRIORS=()
for (( j = 0; j < ${#TEMPLATE_POSTERIORS[@]}; j++ ))
do
POSTERIOR=${TEMPLATE_POSTERIORS[$j]}
if [[ ! -f ${POSTERIOR} ]];
then
TEMPLATE_POSTERIORS_EXIST=0
TEMPLATE_PRIORS_EXIST=0
break;
fi
TEMPLATE_PRIORS[$j]=${POSTERIOR/BrainSegmentationPosteriors/Priors}
if [[ ! -f ${TEMPLATE_PRIORS[$j]} ]];
then
TEMPLATE_PRIORS_EXIST=0
fi
done
if [[ ${TEMPLATE_POSTERIORS_EXIST} -eq 0 ]];
then
echo "Error: Posteriors for the template do not exist."
exit 1
fi
logCmd ImageMath ${DIMENSION} ${TEMPLATE_SKULL_STRIPPED} m ${TEMPLATE_IMAGES[0]} ${TEMPLATE_EXTRACTION_MASK}
logCmd SmoothImage ${DIMENSION} ${TEMPLATE_EXTRACTION_MASK} 1 ${TEMPLATE_EXTRACTION_PRIOR} 1
logCmd ImageMath ${DIMENSION} ${TEMPLATE_EXTRACTION_REGISTRATION_MASK} MD ${TEMPLATE_EXTRACTION_MASK} 40
if [[ ${TEMPLATE_PRIORS_EXIST} -eq 0 ]];
then
if [[ ${#MALF_ATLASES[@]} -eq 0 ]];
then
echo
echo " ---> Smoothing template posteriors as priors."
echo
for j in ${TEMPLATE_POSTERIORS[@]}
do
PRIOR=${j/BrainSegmentationPosteriors/Priors}
logCmd SmoothImage ${DIMENSION} $j 1 $PRIOR 1
done
else
echo
echo " ---> Cooking template priors using antsJointLabelFusion."
echo
TEMPLATE_MALF_LABELS_PREFIX=${OUTPUT_PREFIX}
TEMPLATE_MALF_LABELS=${OUTPUT_PREFIX}Labels.nii.gz
ATLAS_AND_LABELS_STRING=''
for (( j=0; j < ${#MALF_ATLASES[@]}; j++ ))
do
ATLAS_AND_LABELS_STRING="${ATLAS_AND_LABELS_STRING} -g ${MALF_ATLASES[$j]} -l ${MALF_LABELS[$j]}"
done
if [[ ! -f ${TEMPLATE_MALF_LABELS} ]];
then
logCmd antsJointLabelFusion.sh \
-d ${DIMENSION} \
-q ${RUN_FAST_MALF_COOKING} \
-x ${TEMPLATE_EXTRACTION_MASK} \
-c ${DOQSUB} \
-j ${CORES} \
-t ${TEMPLATE_SKULL_STRIPPED} \
-o ${TEMPLATE_MALF_LABELS_PREFIX} \
${ATLAS_AND_LABELS_STRING}
fi
TEMPLATE_PRIORS=()
for (( j = 0; j < ${#TEMPLATE_POSTERIORS[@]}; j++ ))
do
POSTERIOR=${TEMPLATE_POSTERIORS[$j]}
TEMPLATE_PRIORS[$j]=${POSTERIOR/BrainSegmentationPosteriors/Priors}
let PRIOR_LABEL=$j+1
logCmd ThresholdImage ${DIMENSION} ${TEMPLATE_MALF_LABELS} ${TEMPLATE_PRIORS[$j]} ${PRIOR_LABEL} ${PRIOR_LABEL} 1 0
logCmd SmoothImage ${DIMENSION} ${TEMPLATE_PRIORS[$j]} 1 ${TEMPLATE_PRIORS[$j]} 1
done
TMP_CSF_POSTERIOR=${OUTPUT_PREFIX}BrainSegmentationCsfPosteriorTmp.${OUTPUT_SUFFIX}
logCmd SmoothImage ${DIMENSION} ${TEMPLATE_POSTERIORS[0]} 1 ${TMP_CSF_POSTERIOR} 1
logCmd ImageMath ${DIMENSION} ${TEMPLATE_PRIORS[0]} max ${TEMPLATE_PRIORS[0]} ${TMP_CSF_POSTERIOR}
# Clip priors to remove precision errors
logCmd ImageMath ${DIMENSION} ${TEMPLATE_PRIORS[0]} WindowImage ${TEMPLATE_PRIORS[0]} 0 1 0 1
# Brian's finishing touches on "cooking"---subtract out CSF from all other priors
for (( j = 1; j < ${#TEMPLATE_PRIORS[@]}; j++ ))
do
let PRIOR_LABEL=$j+1
logCmd ImageMath ${DIMENSION} ${TEMPLATE_PRIORS[$j]} - ${TEMPLATE_PRIORS[$j]} ${TEMPLATE_PRIORS[0]}
# Clip priors to range [0,1]
logCmd ImageMath ${DIMENSION} ${TEMPLATE_PRIORS[$j]} WindowImage ${TEMPLATE_PRIORS[$j]} 0 1 0 1
done
logCmd rm -f $TMP_CSF_POSTERIOR
logCmd rm -f ${TEMPLATE_MALF_LABELS_PREFIX}*log.txt
fi
fi
time_end_priors=`date +%s`
time_elapsed_priors=$((time_end_priors - time_start_priors))
echo
echo "--------------------------------------------------------------------------------------"
echo " Done with creating template priors: $(( time_elapsed_priors / 3600 ))h $(( time_elapsed_priors %3600 / 60 ))m $(( time_elapsed_priors % 60 ))s"
echo "--------------------------------------------------------------------------------------"
echo
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