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// =============================================================== //
// //
// File : adali.cxx //
// Purpose : alignments //
// //
// Institute of Microbiology (Technical University Munich) //
// http://www.arb-home.de/ //
// //
// =============================================================== //
#include <arbdbt.h>
#include <adGene.h>
#include <arb_strarray.h>
#include "gb_local.h"
static long check_for_species_without_data(const char *species_name, long value, void *counterPtr) {
if (value == 1) {
long cnt = *((long*)counterPtr);
if (cnt<40) {
GB_warningf("Species '%s' has no data in any alignment", species_name);
}
*((long*)counterPtr) = cnt+1;
}
return value; // new hash value
}
GBDATA *GBT_get_presets(GBDATA *gb_main) {
return GBT_find_or_create(gb_main, "presets", 7);
}
int GBT_count_alignments(GBDATA *gb_main) {
int count = 0;
GBDATA *gb_presets = GBT_get_presets(gb_main);
for (GBDATA *gb_ali = GB_entry(gb_presets, "alignment");
gb_ali;
gb_ali = GB_nextEntry(gb_ali))
{
++count;
}
return count;
}
static GB_ERROR GBT_check_alignment(GBDATA *gb_main, GBDATA *preset_alignment, GB_HASH *species_name_hash) {
/* check
* - whether alignment has correct size and
* - whether all data is present.
*
* Sets the security deletes and writes.
*
* If 'species_name_hash' is not NULL,
* - it initially has to contain value == 1 for each existing species.
* - afterwards it will contain value == 2 for each species where an alignment has been found.
*/
GBDATA *gb_species_data = GBT_get_species_data(gb_main);
GBDATA *gb_extended_data = GBT_get_SAI_data(gb_main);
GB_ERROR error = 0;
char *ali_name = GBT_read_string(preset_alignment, "alignment_name");
if (!ali_name) error = "Alignment w/o 'alignment_name'";
if (!error) {
long security_write = -1;
long stored_ali_len = -1;
long found_ali_len = -1;
long aligned = 1;
GBDATA *gb_ali_len = 0;
{
GBDATA *gb_ali_wsec = GB_entry(preset_alignment, "alignment_write_security");
if (!gb_ali_wsec) {
error = "has no 'alignment_write_security' entry";
}
else {
security_write = GB_read_int(gb_ali_wsec);
}
}
if (!error) {
gb_ali_len = GB_entry(preset_alignment, "alignment_len");
if (!gb_ali_len) {
error = "has no 'alignment_len' entry";
}
else {
stored_ali_len = GB_read_int(gb_ali_len);
}
}
if (!error) {
GBDATA *gb_species;
for (gb_species = GBT_first_species_rel_species_data(gb_species_data);
gb_species && !error;
gb_species = GBT_next_species(gb_species))
{
GBDATA *gb_name = GB_entry(gb_species, "name");
const char *name = 0;
int alignment_seen = 0;
if (!gb_name) {
// fatal: name is missing -> create a unique name
char *unique = GBT_create_unique_species_name(gb_main, "autoname.");
error = GBT_write_string(gb_species, "name", unique);
if (!error) {
gb_name = GB_entry(gb_species, "name");
GBS_write_hash(species_name_hash, unique, 1); // not seen before
GB_warningf("Seen unnamed species (gave name '%s')", unique);
}
free(unique);
}
if (!error) {
name = GB_read_char_pntr(gb_name);
if (species_name_hash) {
int seen = GBS_read_hash(species_name_hash, name);
gb_assert(seen != 0); // species_name_hash not initialized correctly
if (seen == 2) alignment_seen = 1; // already seen an alignment
}
}
if (!error) {
GB_push_my_security(gb_name);
error = GB_write_security_delete(gb_name, 7);
if (!error) error = GB_write_security_write(gb_name, 6);
if (!error) {
GBDATA *gb_ali = GB_entry(gb_species, ali_name);
if (gb_ali) {
GBDATA *gb_data = GB_entry(gb_ali, "data");
if (!gb_data) {
error = GBT_write_string(gb_ali, "data", "Error: entry 'data' was missing and therefore was filled with this text.");
GB_warningf("No '%s/data' entry for species '%s' (has been filled with dummy data)", ali_name, name);
}
else {
if (GB_read_type(gb_data) != GB_STRING) {
GB_delete(gb_data);
error = GBS_global_string("'%s/data' of species '%s' had wrong DB-type (%s) and has been deleted!",
ali_name, name, GB_read_key_pntr(gb_data));
}
else {
long data_len = GB_read_string_count(gb_data);
if (found_ali_len != data_len) {
if (found_ali_len>0) aligned = 0;
if (found_ali_len<data_len) found_ali_len = data_len;
}
error = GB_write_security_delete(gb_data, 7);
if (!alignment_seen && species_name_hash) { // mark as seen
GBS_write_hash(species_name_hash, name, 2); // 2 means "species has data in at least 1 alignment"
}
}
}
}
}
if (!error) error = GB_write_security_delete(gb_species, security_write);
GB_pop_my_security(gb_name);
}
}
}
if (!error) {
GBDATA *gb_sai;
for (gb_sai = GBT_first_SAI_rel_SAI_data(gb_extended_data);
gb_sai && !error;
gb_sai = GBT_next_SAI(gb_sai))
{
GBDATA *gb_sai_name = GB_entry(gb_sai, "name");
GBDATA *gb_ali;
if (!gb_sai_name) continue;
GB_write_security_delete(gb_sai_name, 7);
gb_ali = GB_entry(gb_sai, ali_name);
if (gb_ali) {
GBDATA *gb_sai_data;
for (gb_sai_data = GB_child(gb_ali);
gb_sai_data;
gb_sai_data = GB_nextChild(gb_sai_data))
{
long type = GB_read_type(gb_sai_data);
long data_len;
if (type == GB_DB || type < GB_BITS) continue;
if (GB_read_key_pntr(gb_sai_data)[0] == '_') continue; // e.g. _STRUCT (of secondary structure)
data_len = GB_read_count(gb_sai_data);
if (found_ali_len != data_len) {
if (found_ali_len>0) aligned = 0;
if (found_ali_len<data_len) found_ali_len = data_len;
}
}
}
}
}
if (!error && stored_ali_len != found_ali_len) error = GB_write_int(gb_ali_len, found_ali_len);
if (!error) error = GBT_write_int(preset_alignment, "aligned", aligned);
if (error) {
error = GBS_global_string("Error checking alignment '%s':\n%s\n", ali_name, error);
}
}
free(ali_name);
return error;
}
GB_ERROR GBT_check_data(GBDATA *Main, const char *alignment_name) {
/* alignment_name
* == 0 -> check all existing alignments
* otherwise -> check only one alignment
*/
GB_ERROR error = 0;
GBDATA *gb_sd = GBT_get_species_data(Main);
GBDATA *gb_presets = GBT_get_presets(Main);
GB_HASH *species_name_hash = 0;
// create rest of main containers
GBT_get_SAI_data(Main);
GBT_get_tree_data(Main);
if (alignment_name) {
GBDATA *gb_ali_name = GB_find_string(gb_presets, "alignment_name", alignment_name, GB_IGNORE_CASE, SEARCH_GRANDCHILD);
if (!gb_ali_name) {
error = GBS_global_string("Alignment '%s' does not exist - it can't be checked.", alignment_name);
}
}
if (!error) {
// check whether we have an default alignment
GBDATA *gb_use = GB_entry(gb_presets, "use");
if (!gb_use) {
// if we have no default alignment -> look for any alignment
GBDATA *gb_ali_name = GB_find_string(gb_presets, "alignment_name", alignment_name, GB_IGNORE_CASE, SEARCH_GRANDCHILD);
error = gb_ali_name ?
GBT_write_string(gb_presets, "use", GB_read_char_pntr(gb_ali_name)) :
"No alignment defined";
}
}
if (!alignment_name && !error) {
// if all alignments are checked -> use species_name_hash to detect duplicated species and species w/o data
long duplicates = 0;
species_name_hash = GBS_create_hash(GBT_get_species_count(Main), GB_IGNORE_CASE);
if (!error) {
for (GBDATA *gb_species = GBT_first_species_rel_species_data(gb_sd);
gb_species;
gb_species = GBT_next_species(gb_species))
{
const char *name = GBT_read_name(gb_species);
if (GBS_read_hash(species_name_hash, name)) duplicates++;
GBS_incr_hash(species_name_hash, name);
}
}
if (duplicates) {
error = GBS_global_string("Database is corrupted:\n"
"Found %li duplicated species with identical names!\n"
"Fix the problem using\n"
" 'Search For Equal Fields and Mark Duplicates'\n"
"in ARB_NTREE search tool, save DB and restart ARB."
, duplicates);
}
}
if (!error) {
for (GBDATA *gb_ali = GB_entry(gb_presets, "alignment");
gb_ali && !error;
gb_ali = GB_nextEntry(gb_ali))
{
error = GBT_check_alignment(Main, gb_ali, species_name_hash);
}
}
if (species_name_hash) {
if (!error) {
long counter = 0;
GBS_hash_do_loop(species_name_hash, check_for_species_without_data, &counter);
if (counter>0) {
GB_warningf("Found %li species without alignment data (only some were listed)", counter);
}
}
GBS_free_hash(species_name_hash);
}
return error;
}
void GBT_get_alignment_names(ConstStrArray& names, GBDATA *gbd) {
/* Get names of existing alignments from database.
*
* Returns: array of strings, the last element is NULL
* (Note: use GBT_free_names() to free result)
*/
GBDATA *presets = GBT_get_presets(gbd);
for (GBDATA *ali = GB_entry(presets, "alignment"); ali; ali = GB_nextEntry(ali)) {
GBDATA *name = GB_entry(ali, "alignment_name");
names.put(name ? GB_read_char_pntr(name) : "<unnamed alignment>");
}
}
static char *gbt_nonexisting_alignment(GBDATA *gbMain) {
char *ali_other = 0;
int counter;
for (counter = 1; !ali_other; ++counter) {
ali_other = GBS_global_string_copy("ali_x%i", counter);
if (GBT_get_alignment(gbMain, ali_other) != 0) freenull(ali_other); // exists -> continue
}
return ali_other;
}
GB_ERROR GBT_check_alignment_name(const char *alignment_name)
{
GB_ERROR error;
if ((error = GB_check_key(alignment_name))) return error;
if (strncmp(alignment_name, "ali_", 4)) {
return GB_export_errorf("your alignment_name '%s' must start with 'ali_'",
alignment_name);
}
return 0;
}
static GB_ERROR create_ali_strEntry(GBDATA *gb_ali, const char *field, const char *strval, long write_protection) {
GB_ERROR error = 0;
GBDATA *gb_sub = GB_create(gb_ali, field, GB_STRING);
if (!gb_sub) error = GB_await_error();
else {
error = GB_write_string(gb_sub, strval);
if (!error) error = GB_write_security_delete(gb_sub, 7);
if (!error) error = GB_write_security_write(gb_sub, write_protection);
}
if (error) {
error = GBS_global_string("failed to create alignment subentry '%s'\n"
"(Reason: %s)", field, error);
}
return error;
}
static GB_ERROR create_ali_intEntry(GBDATA *gb_ali, const char *field, int intval, long write_protection) {
GB_ERROR error = 0;
GBDATA *gb_sub = GB_create(gb_ali, field, GB_INT);
if (!gb_sub) error = GB_await_error();
else {
error = GB_write_int(gb_sub, intval);
if (!error) error = GB_write_security_delete(gb_sub, 7);
if (!error) error = GB_write_security_write(gb_sub, write_protection);
}
if (error) {
error = GBS_global_string("failed to create alignment subentry '%s'\n"
"(Reason: %s)", field, error);
}
return error;
}
GBDATA *GBT_create_alignment(GBDATA *gbd, const char *name, long len, long aligned, long security, const char *type) {
/* create alignment
*
* returns pointer to alignment or
* NULL (in this case an error has been exported)
*/
GB_ERROR error = NULL;
GBDATA *gb_presets = GBT_get_presets(gbd);
GBDATA *result = NULL;
if (!gb_presets) {
error = GBS_global_string("can't find/create 'presets' (Reason: %s)", GB_await_error());
}
else {
error = GBT_check_alignment_name(name);
if (!error && (security<0 || security>6)) {
error = GBS_global_string("Illegal security value %li (allowed 0..6)", security);
}
if (!error) {
const char *allowed_types = ":dna:rna:ami:usr:";
int tlen = strlen(type);
const char *found = strstr(allowed_types, type);
if (!found || found == allowed_types || found[-1] != ':' || found[tlen] != ':') {
error = GBS_global_string("Invalid alignment type '%s'", type);
}
}
if (!error) {
GBDATA *gb_name = GB_find_string(gb_presets, "alignment_name", name, GB_IGNORE_CASE, SEARCH_GRANDCHILD);
if (gb_name) error = GBS_global_string("Alignment '%s' already exists", name);
else {
GBDATA *gb_ali = GB_create_container(gb_presets, "alignment");
if (!gb_ali) error = GB_await_error();
else {
error = GB_write_security_delete(gb_ali, 6);
if (!error) error = create_ali_strEntry(gb_ali, "alignment_name", name, 6);
if (!error) error = create_ali_intEntry(gb_ali, "alignment_len", len, 0);
if (!error) error = create_ali_intEntry(gb_ali, "aligned", aligned <= 0 ? 0 : 1, 0);
if (!error) error = create_ali_intEntry(gb_ali, "alignment_write_security", security, 6);
if (!error) error = create_ali_strEntry(gb_ali, "alignment_type", type, 0);
}
if (!error) result = gb_ali;
}
}
}
if (!result) {
gb_assert(error);
GB_export_errorf("in GBT_create_alignment: %s", error);
}
#if defined(DEBUG)
else gb_assert(!error);
#endif // DEBUG
return result;
}
static GB_ERROR gbt_rename_alignment_of_item(GBDATA *gb_item_container, const char *item_name, const char *item_entry_name,
const char *source, const char *dest, int copy, int dele)
{
GB_ERROR error = 0;
GBDATA *gb_item;
for (gb_item = GB_entry(gb_item_container, item_entry_name);
gb_item && !error;
gb_item = GB_nextEntry(gb_item))
{
GBDATA *gb_ali = GB_entry(gb_item, source);
if (!gb_ali) continue;
if (copy) {
GBDATA *gb_new = GB_entry(gb_item, dest);
if (gb_new) {
error = GBS_global_string("Entry '%s' already exists", dest);
}
else {
gb_new = GB_create_container(gb_item, dest);
if (!gb_new) error = GB_await_error();
else error = GB_copy(gb_new, gb_ali);
}
}
if (dele) error = GB_delete(gb_ali);
}
if (error && gb_item) {
error = GBS_global_string("%s\n(while renaming alignment for %s '%s')", error, item_name, GBT_read_name(gb_item));
}
return error;
}
GB_ERROR GBT_rename_alignment(GBDATA *gbMain, const char *source, const char *dest, int copy, int dele)
{
/* if copy == 1 then create a copy
* if dele == 1 then delete old
*/
GB_ERROR error = 0;
int is_case_error = 0;
GBDATA *gb_presets = GBT_get_presets(gbMain);
GBDATA *gb_species_data = GBT_get_species_data(gbMain);
GBDATA *gb_extended_data = GBT_get_SAI_data(gbMain);
if (!gb_presets || !gb_species_data || !gb_extended_data) error = GB_await_error();
// create copy and/or delete old alignment description
if (!error) {
GBDATA *gb_old_alignment = GBT_get_alignment(gbMain, source);
if (!gb_old_alignment) {
error = GB_await_error();
}
else {
if (copy) {
GBDATA *gbh = GBT_get_alignment(gbMain, dest);
if (gbh) {
error = GBS_global_string("destination alignment '%s' already exists", dest);
is_case_error = (strcasecmp(source, dest) == 0); // test for case-only difference
}
else {
GB_clear_error();
error = GBT_check_alignment_name(dest);
if (!error) {
GBDATA *gb_new_alignment = GB_create_container(gb_presets, "alignment");
error = GB_copy(gb_new_alignment, gb_old_alignment);
if (!error) error = GBT_write_string(gb_new_alignment, "alignment_name", dest);
}
}
}
if (dele && !error) {
error = GB_delete(gb_old_alignment);
}
}
}
// change default alignment
if (!error && dele && copy) {
error = GBT_write_string(gb_presets, "use", dest);
}
// copy and/or delete alignment entries in species
if (!error) {
error = gbt_rename_alignment_of_item(gb_species_data, "Species", "species", source, dest, copy, dele);
}
// copy and/or delete alignment entries in SAIs
if (!error) {
error = gbt_rename_alignment_of_item(gb_extended_data, "SAI", "extended", source, dest, copy, dele);
}
if (is_case_error) {
// alignments source and dest only differ in case
char *ali_other = gbt_nonexisting_alignment(gbMain);
gb_assert(copy);
printf("Renaming alignment '%s' -> '%s' -> '%s' (to avoid case-problem)\n", source, ali_other, dest);
error = GBT_rename_alignment(gbMain, source, ali_other, 1, dele);
if (!error) error = GBT_rename_alignment(gbMain, ali_other, dest, 1, 1);
free(ali_other);
}
return error;
}
// -----------------------------------------
// alignment related item functions
NOT4PERL GBDATA *GBT_add_data(GBDATA *species, const char *ali_name, const char *key, GB_TYPES type) {
// goes to header: __ATTR__DEPRECATED_TODO("better use GBT_create_sequence_data()")
/* replace this function by GBT_create_sequence_data
* the same as GB_search(species, 'ali_name/key', GB_CREATE)
*
* Note: The behavior is weird, cause it does sth special for GB_STRING (write default content "...")
*
* returns create database entry (or NULL; exports an error in this case)
*/
GB_ERROR error = GB_check_key(ali_name);
if (error) {
error = GBS_global_string("Invalid alignment name '%s' (Reason: %s)", ali_name, error);
}
else {
error = GB_check_hkey(key);
if (error) {
error = GBS_global_string("Invalid field name '%s' (Reason: %s)", key, error);
}
}
GBDATA *gb_data = NULL;
if (error) {
GB_export_error(error);
}
else {
GBDATA *gb_gb = GB_entry(species, ali_name);
if (!gb_gb) gb_gb = GB_create_container(species, ali_name);
if (gb_gb) {
if (type == GB_STRING) {
gb_data = GB_search(gb_gb, key, GB_FIND);
if (!gb_data) {
gb_data = GB_search(gb_gb, key, GB_STRING);
GB_write_string(gb_data, "...");
}
}
else {
gb_data = GB_search(gb_gb, key, type);
}
}
}
return gb_data;
}
NOT4PERL GBDATA *GBT_create_sequence_data(GBDATA *species, const char *ali_name, const char *key, GB_TYPES type, int security_write) {
GBDATA *gb_data = GBT_add_data(species, ali_name, key, type);
if (gb_data) {
GB_ERROR error = GB_write_security_write(gb_data, security_write);
if (error) {
GB_export_error(error);
gb_data = 0;
}
}
return gb_data;
}
GB_ERROR GBT_write_sequence(GBDATA *gb_data, const char *ali_name, long ali_len, const char *sequence) {
/* writes a sequence which is generated by GBT_add_data,
* cuts sequence after alignment len only if bases e ".-nN"
*/
int slen = strlen(sequence);
int old_char = 0;
GB_ERROR error = 0;
if (slen > ali_len) {
int i;
for (i = slen -1; i>=ali_len; i--) {
if (!strchr("-.nN", sequence[i])) break; // real base after end of alignment
}
i++; // points to first 0 after alignment
if (i > ali_len) {
GBDATA *gb_main = GB_get_root(gb_data);
ali_len = GBT_get_alignment_len(gb_main, ali_name);
if (slen > ali_len) { // check for modified alignment len
GBT_set_alignment_len(gb_main, ali_name, i);
ali_len = i;
}
}
if (slen > ali_len) {
old_char = sequence[ali_len];
((char*)sequence)[ali_len] = 0;
}
}
error = GB_write_string(gb_data, sequence);
if (slen> ali_len) ((char*)sequence)[ali_len] = old_char;
return error;
}
GBDATA *GBT_gen_accession_number(GBDATA *gb_species, const char *ali_name) {
GBDATA *gb_acc = GB_entry(gb_species, "acc");
if (!gb_acc) {
GBDATA *gb_data = GBT_read_sequence(gb_species, ali_name);
if (gb_data) { // found a valid alignment
GB_CSTR sequence = GB_read_char_pntr(gb_data);
long id = GBS_checksum(sequence, 1, ".-");
const char *acc = GBS_global_string("ARB_%lX", id);
GB_ERROR error = GBT_write_string(gb_species, "acc", acc);
if (error) GB_export_error(error);
}
}
return gb_acc;
}
int GBT_is_partial(GBDATA *gb_species, int default_value, bool define_if_undef) {
// checks whether a species has a partial or full sequence
//
// Note: partial sequences should not be used for tree calculations
//
// returns: 0 if sequence is full
// 1 if sequence is partial
// -1 in case of error (which is exported in this case)
//
// if the sequence has no 'ARB_partial' entry it returns 'default_value'
// if 'define_if_undef' is true then create an 'ARB_partial'-entry with the default value
int result = -1;
GB_ERROR error = 0;
GBDATA *gb_partial = GB_entry(gb_species, "ARB_partial");
if (gb_partial) {
result = GB_read_int(gb_partial);
if (result != 0 && result != 1) {
error = "Illegal value for 'ARB_partial' (only 1 or 0 allowed)";
}
}
else {
if (define_if_undef) {
error = GBT_write_int(gb_species, "ARB_partial", default_value);
}
result = default_value;
}
if (error) {
GB_export_error(error);
return -1;
}
return result;
}
#if defined(WARN_TODO)
#warning rename GBT_read_sequence - it does not read the sequence itself
#endif
GBDATA *GBT_read_sequence(GBDATA *gb_species, const char *aliname) {
GBDATA *gb_ali = GB_entry(gb_species, aliname);
return gb_ali ? GB_entry(gb_ali, "data") : 0;
}
char *GBT_get_default_alignment(GBDATA *gb_main) {
gb_assert(!GB_have_error()); // illegal to enter this function when an error is exported!
return GBT_read_string(gb_main, GB_DEFAULT_ALIGNMENT);
}
GB_ERROR GBT_set_default_alignment(GBDATA *gb_main, const char *alignment_name) {
return GBT_write_string(gb_main, GB_DEFAULT_ALIGNMENT, alignment_name);
}
GBDATA *GBT_get_alignment(GBDATA *gb_main, const char *aliname) {
GBDATA *gb_presets = GBT_get_presets(gb_main);
GBDATA *gb_alignment_name = GB_find_string(gb_presets, "alignment_name", aliname, GB_IGNORE_CASE, SEARCH_GRANDCHILD);
if (!gb_alignment_name) {
GB_export_errorf("alignment '%s' not found", aliname);
return NULL;
}
return GB_get_father(gb_alignment_name);
}
#if defined(WARN_TODO)
#warning recode and change result type to long* ?
#endif
long GBT_get_alignment_len(GBDATA *gb_main, const char *aliname) {
GBDATA *gb_alignment = GBT_get_alignment(gb_main, aliname);
return gb_alignment ? *GBT_read_int(gb_alignment, "alignment_len") : -1;
}
GB_ERROR GBT_set_alignment_len(GBDATA *gb_main, const char *aliname, long new_len) {
GB_ERROR error = 0;
GBDATA *gb_alignment = GBT_get_alignment(gb_main, aliname);
if (gb_alignment) {
GB_push_my_security(gb_main);
error = GBT_write_int(gb_alignment, "alignment_len", new_len); // write new len
if (!error) error = GBT_write_int(gb_alignment, "aligned", 0); // mark as unaligned
GB_pop_my_security(gb_main);
}
else error = GB_export_errorf("Alignment '%s' not found", aliname);
return error;
}
char *GBT_get_alignment_type_string(GBDATA *gb_main, const char *aliname) {
char *result = NULL;
GBDATA *gb_alignment = GBT_get_alignment(gb_main, aliname);
if (gb_alignment) {
result = GBT_read_string(gb_alignment, "alignment_type");
gb_assert(result);
}
return result;
}
GB_alignment_type GBT_get_alignment_type(GBDATA *gb_main, const char *aliname) {
char *ali_type = GBT_get_alignment_type_string(gb_main, aliname);
GB_alignment_type at = GB_AT_UNKNOWN;
if (ali_type) {
switch (ali_type[0]) {
case 'r': if (strcmp(ali_type, "rna")==0) at = GB_AT_RNA; break;
case 'd': if (strcmp(ali_type, "dna")==0) at = GB_AT_DNA; break;
case 'a': if (strcmp(ali_type, "ami")==0) at = GB_AT_AA; break;
case 'p': if (strcmp(ali_type, "pro")==0) at = GB_AT_AA; break;
default: gb_assert(0); break;
}
free(ali_type);
}
return at;
}
bool GBT_is_alignment_protein(GBDATA *gb_main, const char *alignment_name) {
return GBT_get_alignment_type(gb_main, alignment_name) == GB_AT_AA;
}
// -----------------------
// gene sequence
static const char *gb_cache_genome(GBDATA *gb_genome) {
static GBDATA *gb_last_genome = 0;
static char *last_genome = 0;
if (gb_genome != gb_last_genome) {
free(last_genome);
last_genome = GB_read_string(gb_genome);
gb_last_genome = gb_genome;
}
return last_genome;
}
struct gene_part_pos {
int parts; // initialized for parts
unsigned char *certain; // contains parts "=" chars
char offset[256];
};
static gene_part_pos *gpp = 0;
static void init_gpp(int parts) {
if (!gpp) {
int i;
gpp = (gene_part_pos*)malloc(sizeof(*gpp));
gpp->certain = 0;
for (i = 0; i<256; ++i) gpp->offset[i] = 0;
gpp->offset[(int)'+'] = 1;
gpp->offset[(int)'-'] = -1;
}
else {
if (parts>gpp->parts) freenull(gpp->certain);
}
if (!gpp->certain) {
int forParts = parts+10;
gpp->certain = (unsigned char *)malloc(forParts+1);
memset(gpp->certain, '=', forParts);
gpp->certain[forParts] = 0;
gpp->parts = forParts;
}
}
static void getPartPositions(const GEN_position *pos, int part, size_t *startPos, size_t *stopPos) {
// returns 'startPos' and 'stopPos' of one part of a gene
gb_assert(part<pos->parts);
*startPos = pos->start_pos[part]+gpp->offset[(pos->start_uncertain ? pos->start_uncertain : gpp->certain)[part]];
*stopPos = pos->stop_pos [part]+gpp->offset[(pos->stop_uncertain ? pos->stop_uncertain : gpp->certain)[part]];
}
NOT4PERL char *GBT_read_gene_sequence_and_length(GBDATA *gb_gene, bool use_revComplement, char partSeparator, size_t *gene_length) {
// return the sequence data of a gene
//
// if use_revComplement is true -> use data from complementary strand (if complement is set for gene)
// otherwise -> use data from primary strand (sort+merge parts by position)
//
// if partSeparator not is 0 -> insert partSeparator between single (non-merged) parts
//
// returns sequence as result (and length of sequence if 'gene_length' points to something)
//
// if 'pos_certain' contains '+', start behind position (or end at position)
// '-', start at position (or end before position)
//
// For zero-length genes (e.g. "711^712") this function returns an empty string.
GB_ERROR error = 0;
char *result = 0;
GBDATA *gb_species = GB_get_grandfather(gb_gene);
GEN_position *pos = GEN_read_position(gb_gene);
if (!pos) error = GB_await_error();
else {
GBDATA *gb_seq = GBT_read_sequence(gb_species, "ali_genom");
unsigned long seq_length = GB_read_count(gb_seq);
int p;
int parts = pos->parts;
int resultlen = 0;
int separatorSize = partSeparator ? 1 : 0;
init_gpp(parts);
// test positions and calculate overall result length
for (p = 0; p<parts && !error; p++) {
size_t start;
size_t stop;
getPartPositions(pos, p, &start, &stop);
if (start<1 || start>(stop+1) || stop > seq_length) { // do not reject zero-length genes (start == stop+1)
error = GBS_global_string("Illegal gene position(s): start=%zu, end=%zu, seq.length=%li",
start, stop, seq_length);
}
else {
resultlen += stop-start+1;
}
}
if (separatorSize) resultlen += (parts-1)*separatorSize;
if (!error) {
char T_or_U = 0;
if (use_revComplement) {
error = GBT_determine_T_or_U(GB_AT_DNA, &T_or_U, "reverse-complement");
}
else if (parts>1) {
GEN_sortAndMergeLocationParts(pos);
parts = pos->parts; // may have changed
}
if (!error) {
const char *seq_data = gb_cache_genome(gb_seq);
char *resultpos;
result = (char*)malloc(resultlen+1);
resultpos = result;
if (gene_length) *gene_length = resultlen;
for (p = 0; p<parts; ++p) {
size_t start;
size_t stop;
getPartPositions(pos, p, &start, &stop);
int len = stop-start+1;
if (separatorSize && p>0) *resultpos++ = partSeparator;
memcpy(resultpos, seq_data+start-1, len);
if (T_or_U && pos->complement[p]) {
GBT_reverseComplementNucSequence(resultpos, len, T_or_U);
}
resultpos += len;
}
resultpos[0] = 0;
}
}
GEN_free_position(pos);
}
gb_assert(result || error);
if (error) {
char *id = GEN_global_gene_identifier(gb_gene, gb_species);
error = GB_export_errorf("Can't read sequence of '%s' (Reason: %s)", id, error);
free(id);
free(result);
result = 0;
}
return result;
}
char *GBT_read_gene_sequence(GBDATA *gb_gene, bool use_revComplement, char partSeparator) {
return GBT_read_gene_sequence_and_length(gb_gene, use_revComplement, partSeparator, 0);
}
// --------------------------------------------------------------------------------
#ifdef UNIT_TESTS
#include <test_unit.h>
void TEST_alignment() {
GB_shell shell;
GBDATA *gb_main = GB_open("TEST_prot.arb", "r");
{
GB_transaction ta(gb_main);
TEST_EXPECT_EQUAL(GBT_count_alignments(gb_main), 2);
char *def_ali_name = GBT_get_default_alignment(gb_main);
TEST_EXPECT_EQUAL(def_ali_name, "ali_tuf_dna");
{
ConstStrArray names;
GBT_get_alignment_names(names, gb_main);
{
char *joined = GBT_join_names(names, '*');
TEST_EXPECT_EQUAL(joined, "ali_tuf_pro*ali_tuf_dna");
free(joined);
}
for (int i = 0; names[i]; ++i) {
long len = GBT_get_alignment_len(gb_main, names[i]);
TEST_EXPECT_EQUAL(len, !i ? 473 : 1426);
char *type_name = GBT_get_alignment_type_string(gb_main, names[i]);
TEST_EXPECT_EQUAL(type_name, !i ? "ami" : "dna");
free(type_name);
GB_alignment_type type = GBT_get_alignment_type(gb_main, names[i]);
TEST_EXPECT_EQUAL(type, !i ? GB_AT_AA : GB_AT_DNA);
TEST_EXPECT_EQUAL(GBT_is_alignment_protein(gb_main, names[i]), !i);
}
}
free(def_ali_name);
}
GB_close(gb_main);
}
#endif // UNIT_TESTS
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