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// ============================================================= //
// //
// File : arb_consensus_tree.cxx //
// Purpose : build consensus tree with the same library //
// as ARB-NJ //
// //
// Coded by Ralf Westram (coder@reallysoft.de) in March 2012 //
// Institute of Microbiology (Technical University Munich) //
// http://www.arb-home.de/ //
// //
// ============================================================= //
#include <CT_ctree.hxx>
#include <TreeRead.h>
#include <TreeWrite.h>
#include <arb_str.h>
#include <arb_diff.h>
#include <arb_defs.h>
using namespace std;
static GBT_TREE *build_consensus_tree(const CharPtrArray& input_trees, GB_ERROR& error, size_t& different_species, double weight, char *&comment) {
// read all input trees, generate and return consensus tree
// (Note: the 'weight' used here doesn't matter atm, since all trees are added with the same weight)
arb_assert(!error);
error = NULL;
comment = NULL;
GBT_TREE *consense_tree = NULL;
if (input_trees.empty()) {
error = "no trees given";
}
else {
ConsensusTreeBuilder tree_builder;
for (size_t i = 0; !error && i<input_trees.size(); ++i) {
char *warnings = NULL;
TreeRoot *root = new TreeRoot(new SizeAwareNodeFactory, true); // will be deleted when tree gets deleted
SizeAwareTree *tree = DOWNCAST(SizeAwareTree*, TREE_load(input_trees[i], *root, NULL, true, &warnings));
if (!tree) {
error = GBS_global_string("Failed to load tree '%s' (Reason: %s)", input_trees[i], GB_await_error());
}
else {
if (warnings) {
GB_warningf("while loading tree '%s':\n%s", input_trees[i], warnings);
free(warnings);
}
tree_builder.add(tree, input_trees[i], weight);
}
}
if (!error) consense_tree = tree_builder.get(different_species, error);
if (!error) comment = tree_builder.get_remark();
}
arb_assert(contradicted(consense_tree, error));
return consense_tree;
}
static char *create_tree_name(const char *savename) {
// create a DB treename (using savename as hint)
char *tree_name;
{
// as default use part behind '/' and remove file extension
const char *lslash = strrchr(savename, '/');
if (lslash) savename = lslash+1;
const char *ldot = strrchr(savename, '.');
tree_name = ldot ? GB_strpartdup(savename, ldot-1) : strdup(savename);
if (tree_name[0] == 0) freedup(tree_name, "tree_consensus");
}
// make sure tree name starts with 'tree_'
if (!ARB_strBeginsWith(tree_name, "tree_")) {
freeset(tree_name, GBS_global_string_copy("tree_%s", tree_name));
}
return tree_name;
}
static GB_ERROR save_tree_as_newick(GBT_TREE *tree, const char *savename, const char *comment) {
// save a tree to a newick file
// since ARB only saves trees out of a database,
// i use a hack here:
// - create temp DB
// - save tree there
// - save to newick as usual
GB_shell shell;
GBDATA *gb_main = GB_open("", "crw");
GB_ERROR error = NULL;
if (!gb_main) {
error = GB_await_error();
}
else {
char *db_tree_name = create_tree_name(savename);
{
GB_transaction ta(gb_main);
error = GBT_write_tree_with_remark(gb_main, db_tree_name, tree, comment);
}
if (!error) error = TREE_write_Newick(gb_main, db_tree_name, NULL, true, true, true, true, TREE_SINGLE_QUOTES, savename);
free(db_tree_name);
GB_close(gb_main);
}
if (error) {
error = GBS_global_string("Failed to save tree to '%s' (Reason: %s)", savename, error);
}
return error;
}
int ARB_main(int argc, char *argv[]) {
GB_ERROR error = NULL;
if (argc<2) {
printf("Usage: arb_consensus_tree [options] [tree]+\n"
"Purpose: Create a consensus tree out of multiple trees\n"
" options:\n"
" -w outfile write consensus tree to outfile\n");
// @@@ wanted options
// - do not add relative frequency of used subtrees as bootstrap values
// - multifurcate branches with bootstrap value below XXX
// - eliminate branches with bootstrap value below YYY
// - ... ?
}
else {
char *savename = NULL;
ConstStrArray input_tree_names;
for (int a = 1; a<argc; ++a) {
const char *arg = argv[a];
if (arg[0] == '-') {
switch (arg[1]) {
case 'w': savename = strdup(argv[++a]); break;
default : error = GBS_global_string("Unknown switch '-%c'", arg[1]); break;
}
}
else {
input_tree_names.put(argv[a]);
}
}
if (!error && input_tree_names.empty()) error = "no input trees specified";
if (!error) {
size_t species_count;
char *comment;
GBT_TREE *cons_tree = build_consensus_tree(input_tree_names, error, species_count, 1.0, comment);
if (!cons_tree) {
error = GBS_global_string("Failed to build consensus tree (Reason: %s)", error);
}
else {
size_t leafs = GBT_count_leafs(cons_tree);
double percent = size_t((leafs*1000)/species_count)/10.0;
printf("Generated tree contains %.1f%% of species (%zu of %zu found in input trees)\n",
percent, leafs, species_count);
if (savename) {
error = save_tree_as_newick(cons_tree, savename, comment);
}
else {
printf("successfully created consensus tree\n"
"(no savename specified -> tree not saved)\n");
}
delete cons_tree;
}
free(comment);
}
free(savename);
}
if (error) {
printf("Error in arb_consensus_tree: %s\n", error);
}
return error ? EXIT_FAILURE : EXIT_SUCCESS;
}
// --------------------------------------------------------------------------------
#ifdef UNIT_TESTS
#ifndef TEST_UNIT_H
#include <test_unit.h>
#endif
#include "command_output.h"
// #define TEST_AUTO_UPDATE // uncomment to update expected trees (if more than date differs)
static char *custom_tree_name(int dir, const char *name) { return GBS_global_string_copy("consense/%i/%s.tree", dir, name); }
static char *custom_numbered_tree_name(int dir, const char *name, int treeNr) { return GBS_global_string_copy("consense/%i/%s_%i.tree", dir, name, treeNr); }
static void add_inputnames(StrArray& to, int dir, const char *basename, int first_tree, int last_tree) {
for (int t = first_tree; t <= last_tree; ++t) {
to.put(custom_numbered_tree_name(dir, basename, t));
}
}
static double calc_intree_distance(GBT_TREE *tree) {
if (tree->is_leaf) return 0.0;
return
tree->leftlen +
tree->rightlen +
calc_intree_distance(tree->leftson) +
calc_intree_distance(tree->rightson);
}
#define LENSUM_EPSILON .000001
static arb_test::match_expectation consense_tree_generated(GBT_TREE *tree, GB_ERROR error, size_t species_count, size_t expected_species_count, double expected_intree_distance) {
using namespace arb_test;
expectation_group expected;
expected.add(that(error).is_equal_to_NULL());
expected.add(that(tree).does_differ_from_NULL());
if (tree) {
expected.add(that(species_count).is_equal_to(expected_species_count));
expected.add(that(GBT_count_leafs(tree)).is_equal_to(expected_species_count));
expected.add(that(calc_intree_distance(tree)).fulfills(epsilon_similar(LENSUM_EPSILON), expected_intree_distance));
}
return all().ofgroup(expected);
}
static arb_test::match_expectation build_expected_consensus_tree(const int treedir, const char *basename, int first_tree, int last_tree, double weight, const char *outbasename, size_t expected_species_count, double expected_intree_distance) {
using namespace arb_test;
expectation_group expected;
arb_suppress_progress hideProgress;
GB_ERROR error = NULL;
StrArray input_tree_names;
add_inputnames(input_tree_names, treedir, basename, first_tree, last_tree);
size_t species_count;
char *comment;
GBT_TREE *tree = build_consensus_tree(input_tree_names, error, species_count, weight, comment);
expected.add(consense_tree_generated(tree, error, species_count, expected_species_count, expected_intree_distance));
char *saveas = custom_tree_name(treedir, outbasename);
error = save_tree_as_newick(tree, saveas, comment);
expected.add(that(error).is_equal_to_NULL());
if (!error) {
char *expected_save = custom_tree_name(treedir, GBS_global_string("%s_expected", outbasename));
bool exported_as_expected = arb_test::textfiles_have_difflines_ignoreDates(expected_save, saveas, 0);
#if defined(TEST_AUTO_UPDATE)
if (!exported_as_expected) {
ASSERT_RESULT(int, 0, system(GBS_global_string("cp %s %s", saveas, expected_save)));
}
#else // !defined(TEST_AUTO_UPDATE)
expected.add(that(exported_as_expected).is_equal_to(true));
#endif
TEST_EXPECT_ZERO_OR_SHOW_ERRNO(GB_unlink(saveas));
free(expected_save);
}
free(saveas);
free(comment);
delete tree;
return all().ofgroup(expected);
}
void TEST_consensus_tree_1() {
TEST_EXPECTATION(build_expected_consensus_tree(1, "bootstrapped", 1, 5, 0.7, "consense1", 22, 0.925779));
// ../UNIT_TESTER/run/consense/1/consense1.tree
}
void TEST_consensus_tree_1_single() {
TEST_EXPECTATION(build_expected_consensus_tree(1, "bootstrapped", 1, 1, 0.01, "consense1_single", 22, 0.924610));
// ../UNIT_TESTER/run/consense/1/consense1_single.tree
}
void TEST_consensus_tree_2() {
TEST_EXPECTATION(build_expected_consensus_tree(2, "bootstrapped", 1, 4, 2.5, "consense2", 59, 2.849827));
// ../UNIT_TESTER/run/consense/2/consense2.tree
}
void TEST_consensus_tree_3() {
TEST_EXPECTATION(build_expected_consensus_tree(3, "bootstrapped", 1, 3, 137.772, "consense3", 128, 2.170685));
// ../UNIT_TESTER/run/consense/3/consense3.tree
}
void TEST_consensus_tree_from_disjunct_trees() {
TEST_EXPECTATION(build_expected_consensus_tree(4, "disjunct", 1, 2, 137.772, "disjunct_merged", 15, 2.034290));
// ../UNIT_TESTER/run/consense/4/disjunct_merged.tree
}
void TEST_consensus_tree_from_partly_overlapping_trees() {
// tree_disjunct_3 contains 7 species
// (3 from upper subtree (tree_disjunct_1) and 4 from lower subtree (tree_disjunct_2))
TEST_EXPECTATION(build_expected_consensus_tree(4, "disjunct", 1, 3, 137.772, "overlap_merged", 15, 2.596455));
// ../UNIT_TESTER/run/consense/4/overlap_merged.tree
}
void TEST_consensus_tree_from_minimal_overlapping_trees() {
// tree_disjunct_0 only contains 2 species (1 from upper and 1 from lower subtree).
TEST_EXPECTATION(build_expected_consensus_tree(4, "disjunct", 0, 2, 137.772, "overlap_mini_merged", 15, 2.750745));
// ../UNIT_TESTER/run/consense/4/overlap_mini_merged.tree
}
void TEST_consensus_tree_described_in_arbhelp() {
// see ../HELP_SOURCE/oldhelp/consense_algo.hlp
TEST_EXPECTATION(build_expected_consensus_tree(5, "help", 1, 2, 2.0, "help_merged", 6, 1.050000));
// ../UNIT_TESTER/run/consense/5/help_merged.tree
}
void TEST_consensus_tree_from_trees_overlapping_by_twothirds() {
// These 3 trees where copied from an existing tree.
// From each copy one third of all species has been removed
// (removed sets were disjunct)
TEST_EXPECTATION(build_expected_consensus_tree(6, "overlap_two_thirds", 1, 3, 19.2, "overlap_twothirds_merged", 15, 3.561680));
// ../UNIT_TESTER/run/consense/6/overlap_twothirds_merged.tree
}
void TEST_consensus_tree_from_mostly_overlapping_trees() {
// the 3 trees were copied from tree_disjunct_source.
// from each tree 2 (different) species were deleted.
TEST_EXPECTATION(build_expected_consensus_tree(7, "disjunct_del2", 1, 3, 137.772, "overlap_mostly", 15, 1.820057));
// ../UNIT_TESTER/run/consense/7/overlap_mostly.tree
}
void TEST_consensus_tree_from_mostly_overlapping_trees_2() {
// the 3 trees were copied from tree_disjunct1
// from each tree 1 (different) species was deleted.
TEST_EXPECTATION(build_expected_consensus_tree(8, "overlap2", 1, 3, 137.772, "overlap2_mostly", 8, 0.529109));
// ../UNIT_TESTER/run/consense/8/overlap2_mostly.tree
}
#define REPEATED_TESTS
#if defined(REPEATED_TESTS)
void TEST_consensus_tree_generation_is_deterministic() {
TEST_consensus_tree_described_in_arbhelp();
TEST_consensus_tree_from_minimal_overlapping_trees();
TEST_consensus_tree_from_partly_overlapping_trees();
TEST_consensus_tree_from_disjunct_trees();
TEST_consensus_tree_3();
TEST_consensus_tree_2();
TEST_consensus_tree_1_single();
TEST_consensus_tree_1();
}
void TEST_arb_consensus_tree() {
TEST_STDOUT_CONTAINS("(arb_consensus_tree -x || true)", "Unknown switch '-x'");
TEST_STDOUT_CONTAINS("(arb_consensus_tree -w sth || true)", "no input trees specified");
{
char *saveas = custom_tree_name(1, "consense1");
char *expected = custom_tree_name(1, "consense1_expected");
TEST_STDOUT_CONTAINS("arb_consensus_tree"
" -w consense/1/consense1.tree"
" consense/1/bootstrapped_1.tree"
" consense/1/bootstrapped_2.tree"
" consense/1/bootstrapped_3.tree"
" consense/1/bootstrapped_4.tree"
" consense/1/bootstrapped_5.tree"
,
"database created");
TEST_EXPECT_TEXTFILE_DIFFLINES_IGNORE_DATES(saveas, expected, 0);
TEST_EXPECT_ZERO_OR_SHOW_ERRNO(GB_unlink(saveas));
free(expected);
free(saveas);
}
{
char *saveas = custom_tree_name(2, "consense2");
char *expected = custom_tree_name(2, "consense2_expected");
TEST_STDOUT_CONTAINS("arb_consensus_tree"
" -w consense/2/consense2.tree"
" consense/2/bootstrapped_1.tree"
" consense/2/bootstrapped_2.tree"
" consense/2/bootstrapped_3.tree"
" consense/2/bootstrapped_4.tree"
,
"database created");
TEST_EXPECT_TEXTFILE_DIFFLINES_IGNORE_DATES(saveas, expected, 0);
TEST_EXPECT_ZERO_OR_SHOW_ERRNO(GB_unlink(saveas));
free(expected);
free(saveas);
}
}
#endif // REPEATED_TESTS
// #define TREEIO_AUTO_UPDATE // uncomment to auto-update expected test-results
// #define TREEIO_AUTO_UPDATE_IF_EXPORT_DIFFERS // uncomment to auto-update expected test-results
// #define TREEIO_AUTO_UPDATE_IF_REEXPORT_DIFFERS // uncomment to auto-update expected test-results
static const char *findFirstNameContaining(GBT_TREE *tree, const char *part) {
const char *found = NULL;
if (tree->name && strstr(tree->name, part)) {
found = tree->name;
}
else if (!tree->is_leaf) {
found = findFirstNameContaining(tree->leftson, part);
if (!found) found = findFirstNameContaining(tree->rightson, part);
}
return found;
}
void TEST_SLOW_treeIO_stable() {
const char *dbname = "trees/bootstrap_groups.arb";
const char *treename = "tree_bootstrap_and_groups";
const char *savename = "bg";
GB_shell shell;
GBDATA *gb_main = GB_open(dbname, "rw");
TEST_REJECT_NULL(gb_main);
GBT_TREE_NodeFactory nodeMaker;
char *outfile = GBS_global_string_copy("trees/%s.tree", savename);
for (int save_branchlengths = 0; save_branchlengths <= 1; ++save_branchlengths) {
for (int save_bootstraps = 0; save_bootstraps <= 1; ++save_bootstraps) {
for (int save_groupnames = 0; save_groupnames <= 1; ++save_groupnames) {
bool quoting_occurs = save_bootstraps && save_groupnames;
for (int pretty = 0; pretty <= 1; ++pretty) {
for (int quoting = TREE_DISALLOW_QUOTES; quoting <= (quoting_occurs ? TREE_DOUBLE_QUOTES : TREE_DISALLOW_QUOTES); ++quoting) {
TREE_node_quoting quoteMode = TREE_node_quoting(quoting);
char *paramID = GBS_global_string_copy("%s_%s%s%s_%i",
pretty ? "p" : "s",
save_bootstraps ? "Bs" : "",
save_groupnames ? "Grp" : "",
save_branchlengths ? "Len" : "",
quoteMode);
TEST_ANNOTATE(GBS_global_string("for paramID='%s'", paramID));
GB_ERROR export_error = TREE_write_Newick(gb_main, treename, NULL, save_branchlengths, save_bootstraps, save_groupnames, pretty, quoteMode, outfile);
TEST_EXPECT_NULL(export_error);
char *expectedfile = GBS_global_string_copy("trees/%s_exp_%s.tree", savename, paramID);
#if defined(TREEIO_AUTO_UPDATE)
system(GBS_global_string("cp %s %s", outfile, expectedfile));
#else // !defined(TREEIO_AUTO_UPDATE)
bool exported_as_expected = arb_test::textfiles_have_difflines_ignoreDates(expectedfile, outfile, 0);
#if defined(TREEIO_AUTO_UPDATE_IF_EXPORT_DIFFERS)
if (!exported_as_expected) {
system(GBS_global_string("cp %s %s", outfile, expectedfile));
}
#else // !defined(TREEIO_AUTO_UPDATE_IF_EXPORT_DIFFERS)
TEST_EXPECT(exported_as_expected);
#endif
// reimport exported tree
const char *reloaded_treename = "tree_reloaded";
{
char *comment = NULL;
GBT_TREE *tree = TREE_load(expectedfile, nodeMaker, &comment, true, NULL);
GB_ERROR load_error = tree ? NULL : GB_await_error();
TEST_EXPECTATION(all().of(that(tree).does_differ_from_NULL(),
that(load_error).is_equal_to_NULL()));
// store tree in DB
{
GB_transaction ta(gb_main);
GB_ERROR store_error = GBT_write_tree_with_remark(gb_main, reloaded_treename, tree, comment);
TEST_EXPECT_NULL(store_error);
}
free(comment);
if (save_groupnames) {
const char *quotedGroup = findFirstNameContaining(tree, "quoted");
const char *underscoreGroup = findFirstNameContaining(tree, "bs100");
TEST_EXPECT_EQUAL(quotedGroup, "quoted");
TEST_EXPECT_EQUAL(underscoreGroup, "__bs100");
}
const char *capsLeaf = findFirstNameContaining(tree, "Caps");
TEST_EXPECT_EQUAL(capsLeaf, "_MhuCaps");
delete tree;
}
// export again
GB_ERROR reexport_error = TREE_write_Newick(gb_main, reloaded_treename, NULL, save_branchlengths, save_bootstraps, save_groupnames, pretty, quoteMode, outfile);
TEST_EXPECT_NULL(reexport_error);
// eliminate comments added by loading/saving
char *outfile2 = GBS_global_string_copy("trees/%s2.tree", savename);
{
char *cmd = GBS_global_string_copy("cat %s"
" | grep -v 'Loaded from trees/.*_exp_'"
" | grep -v 'tree_reloaded saved to'"
" > %s", outfile, outfile2);
TEST_EXPECT_NO_ERROR(GBK_system(cmd));
free(cmd);
}
bool reexported_as_expected = arb_test::textfiles_have_difflines(expectedfile, outfile2, 0);
#if defined(TREEIO_AUTO_UPDATE_IF_REEXPORT_DIFFERS)
if (!reexported_as_expected) {
system(GBS_global_string("cp %s %s", outfile2, expectedfile));
}
#else // !defined(TREEIO_AUTO_UPDATE_IF_REEXPORT_DIFFERS)
TEST_EXPECT(reexported_as_expected);
#endif
TEST_EXPECT_ZERO_OR_SHOW_ERRNO(unlink(outfile2));
free(outfile2);
#endif
free(expectedfile);
free(paramID);
}
}
}
}
}
TEST_ANNOTATE(NULL);
TEST_EXPECT_ZERO_OR_SHOW_ERRNO(unlink(outfile));
free(outfile);
GB_close(gb_main);
}
void TEST_CONSENSUS_TREE_functionality() {
// functionality wanted in RootedTree (for use in library CONSENSUS_TREE)
char *comment = NULL;
SizeAwareTree *tree = DOWNCAST(SizeAwareTree*, TREE_load("trees/bg_exp_p_GrpLen_0.tree",
*new TreeRoot(new SizeAwareNodeFactory, true),
&comment, false, NULL));
// -> ../UNIT_TESTER/run/trees/bg_exp_p_GrpLen_0.tree
#define ORG_1111 "(AticSea6,(RblAerol,RblMesop))"
#define TOP_1111 "((RblAerol,RblMesop),AticSea6)"
#define BOT_1111 ORG_1111
#define ORG_11121 "((DnrShiba,RsbElon4),MmbAlkal)"
#define TOP_11121 ORG_11121
#define BOT_11121 "(MmbAlkal,(DnrShiba,RsbElon4))"
#define ORG_11122 "((MabPelag,MabSalin),PaoMaris)"
#define TOP_11122 ORG_11122
#define BOT_11122 "(PaoMaris,(MabPelag,MabSalin))"
#define ORG_1112 "(" ORG_11121 "," ORG_11122 ")"
#define TOP_1112 "(" TOP_11121 "," TOP_11122 ")"
#define BOT_1112 "(" BOT_11121 "," BOT_11122 ")"
#define EDG_1112 "(" TOP_11121 "," BOT_11122 ")"
#define ORG_111 "(" ORG_1111 "," ORG_1112 ")"
#define TOP_111 "(" TOP_1112 "," TOP_1111 ")"
#define BOT_111 "(" BOT_1111 "," BOT_1112 ")"
#define EDG_111 "(" EDG_1112 "," BOT_1111 ")"
#define ORG_112 "(OnlGran2,RsnAnta2)"
#define TOP_112 ORG_112
#define BOT_112 ORG_112
#define ORG_11 "(" ORG_111 "," ORG_112 ")"
#define TOP_11 "(" TOP_111 "," TOP_112 ")"
#define BOT_11 "(" BOT_112 "," BOT_111 ")"
#define EDG_11 "(" EDG_111 "," BOT_112 ")"
#define ORG_12 "(_MhuCaps,ThtNivea)"
#define TOP_12 "(ThtNivea,_MhuCaps)"
#define BOT_12 TOP_12
#define ORG_1 "(" ORG_11 "," ORG_12 ")"
#define TOP_1 "(" TOP_11 "," TOP_12 ")"
#define BOT_1 "(" BOT_12 "," BOT_11 ")"
#define EDG_1 "(" EDG_11 "," BOT_12 ")"
#define ORG_2 "((LbnMarin,LbnzAlb4),LbnAlexa)"
#define TOP_2 ORG_2
#define BOT_2 "(LbnAlexa,(LbnMarin,LbnzAlb4))"
// test swap_sons
TEST_ASSERT_VALID_TREE(tree);
TEST_EXPECT_NEWICK(nSIMPLE, tree, "(" ORG_1 "," ORG_2 ");");
tree->swap_sons();
TEST_EXPECT_NEWICK(nSIMPLE, tree, "(" ORG_2 "," ORG_1 ");");
// test reorder_tree
TEST_ASSERT_VALID_TREE(tree);
TreeOrder order[] = { BIG_BRANCHES_TO_TOP, BIG_BRANCHES_TO_BOTTOM, BIG_BRANCHES_TO_EDGE };
for (size_t o1 = 0; o1<ARRAY_ELEMS(order); ++o1) {
TreeOrder to_order = order[o1];
for (size_t o2 = 0; o2<ARRAY_ELEMS(order); ++o2) {
TreeOrder from_order = order[o2];
for (int rotate = 0; rotate<=1; ++rotate) {
tree->reorder_tree(from_order);
if (rotate) tree->rotate_subtree();
tree->reorder_tree(to_order);
switch (to_order) {
case BIG_BRANCHES_TO_TOP: TEST_EXPECT_NEWICK(nSIMPLE, tree, "(" TOP_1 "," TOP_2 ");"); break;
case BIG_BRANCHES_TO_EDGE: TEST_EXPECT_NEWICK(nSIMPLE, tree, "(" EDG_1 "," BOT_2 ");"); break;
case BIG_BRANCHES_TO_BOTTOM: TEST_EXPECT_NEWICK(nSIMPLE, tree, "(" BOT_2 "," BOT_1 ");"); break;
default: TEST_REJECT(true); break;
}
}
}
}
// test rotate_subtree
TEST_ASSERT_VALID_TREE(tree);
tree->reorder_tree(BIG_BRANCHES_TO_TOP);
tree->rotate_subtree(); TEST_EXPECT_NEWICK(nSIMPLE, tree, "((LbnAlexa,(LbnzAlb4,LbnMarin)),((_MhuCaps,ThtNivea),((RsnAnta2,OnlGran2),((AticSea6,(RblMesop,RblAerol)),((PaoMaris,(MabSalin,MabPelag)),(MmbAlkal,(RsbElon4,DnrShiba)))))));");
tree->rotate_subtree(); TEST_EXPECT_NEWICK(nSIMPLE, tree, "(" TOP_1 "," TOP_2 ");");
// test set_root
TEST_ASSERT_VALID_TREE(tree);
RootedTree *AticSea6Grandpa = tree->findLeafNamed("AticSea6")->get_father()->get_father();
TEST_REJECT_NULL(AticSea6Grandpa);
TEST_ASSERT_VALID_TREE(AticSea6Grandpa);
AticSea6Grandpa->set_root();
TEST_EXPECT_NEWICK(nSIMPLE, tree,
"((" ORG_1112 "," TOP_1111 ")," // AticSea6 is direct son of TOP_1111
"((" ORG_2 "," TOP_12 ")," ORG_112 "));");
// test auto-detection of "best" root
TEST_ASSERT_VALID_TREE(tree);
tree->get_tree_root()->find_innermost_edge().set_root();
TEST_EXPECT_NEWICK(nLENGTH, tree,
"((((LbnMarin:0.019,LbnzAlb4:0.003):0.016,LbnAlexa:0.032):0.122,(ThtNivea:0.230,_MhuCaps:0.194):0.427):0.076,"
"(((((DnrShiba:0.076,RsbElon4:0.053):0.034,MmbAlkal:0.069):0.016,((MabPelag:0.001,MabSalin:0.009):0.095,PaoMaris:0.092):0.036):0.030,((RblAerol:0.085,RblMesop:0.042):0.238,AticSea6:0.111):0.018):0.036,(OnlGran2:0.057,RsnAnta2:0.060):0.021):0.076);");
TEST_ASSERT_VALID_TREE(tree);
delete tree;
free(comment);
}
#endif // UNIT_TESTS
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