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// =============================================================== //
// //
// File : ColumnStat_2_gnuplot.cxx //
// Purpose : //
// //
// Institute of Microbiology (Technical University Munich) //
// http://www.arb-home.de/ //
// //
// =============================================================== //
#include "NT_local.h"
#include <ColumnStat.hxx>
#include <AP_filter.hxx>
#include <awt_filter.hxx>
#include <aw_awars.hxx>
#include <aw_file.hxx>
#include <aw_msg.hxx>
#include <aw_root.hxx>
#include <aw_select.hxx>
#include <arbdbt.h>
#include <arb_file.h>
#include <unistd.h>
#define AWAR_CS2GP "tmp/ntree/colstat_2_gnuplot"
#define AWAR_CS2GP_NAME AWAR_CS2GP "/name"
#define AWAR_CS2GP_SUFFIX AWAR_CS2GP "/filter"
#define AWAR_CS2GP_DIRECTORY AWAR_CS2GP "/directory"
#define AWAR_CS2GP_FILENAME AWAR_CS2GP "/file_name"
#define AWAR_CS2GP_SMOOTH_VALUES AWAR_CS2GP "/smooth_values"
#define AWAR_CS2GP_SMOOTH_GNUPLOT AWAR_CS2GP "/smooth_gnuplot"
#define AWAR_CS2GP_GNUPLOT_OVERLAY_PREFIX AWAR_CS2GP "/gnuplot_overlay_prefix"
#define AWAR_CS2GP_GNUPLOT_OVERLAY_POSTFIX AWAR_CS2GP "/gnuplot_overlay_postfix"
#define AWAR_CS2GP_FILTER_NAME AWAR_CS2GP "/ap_filter/name"
#define AWAR_CS2GP_FILTER_ALIGNMENT AWAR_CS2GP "/ap_filter/alignment"
#define AWAR_CS2GP_FILTER_FILTER AWAR_CS2GP "/ap_filter/filter"
static GB_ERROR split_stat_filename(const char *fname, char **dirPtr, char **name_prefixPtr, char **name_postfixPtr) {
// 'fname' is sth like 'directory/prefix.sth_gnu'
// 'dirPtr' is set to a malloc-copy of 'directory'
// 'name_prefixPtr' is set to a malloc-copy of 'prefix' (defaults to '*')
// 'name_postfixPtr' is set to a malloc-copy of 'sth_gnu' (defaults to '*_gnu')
*dirPtr = 0;
*name_prefixPtr = 0;
*name_postfixPtr = 0;
const char *lslash = strrchr(fname, '/');
if (!lslash) return GB_export_errorf("'%s' has to contain a '/'", fname);
char *dir = strdup(fname);
dir[lslash-fname] = 0; // cut off at last '/'
char *name_prefix = strdup(lslash+1);
char *name_postfix = 0;
char *ldot = strrchr(name_prefix, '.');
if (ldot) {
ldot[0] = 0;
name_postfix = strdup(ldot+1);
}
if (!ldot || name_prefix[0] == 0) freedup(name_prefix, "*"); // no dot or empty name_prefix
if (!name_postfix || name_postfix[0] == 0) freedup(name_postfix, "*_gnu");
nt_assert(name_prefix);
nt_assert(name_postfix);
*dirPtr = dir;
*name_prefixPtr = name_prefix;
*name_postfixPtr = name_postfix;
return 0;
}
static char * get_overlay_files(AW_root *awr, const char *fname, GB_ERROR& error) {
nt_assert(!error);
bool overlay_prefix = awr->awar(AWAR_CS2GP_GNUPLOT_OVERLAY_PREFIX)->read_int();
bool overlay_postfix = awr->awar(AWAR_CS2GP_GNUPLOT_OVERLAY_POSTFIX)->read_int();
char *dir, *name_prefix, *name_postfix;
error = split_stat_filename(fname, &dir, &name_prefix, &name_postfix);
char *found_files = 0;
if (!error) {
char *found_prefix_files = 0;
char *found_postfix_files = 0;
if (overlay_prefix || overlay_postfix) {
char *mask = GBS_global_string_copy("%s.*_gnu", name_prefix);
if (overlay_prefix) {
#if defined(WARN_TODO)
#warning change error handling for GB_find_all_files() - globally!
#endif
found_prefix_files = GB_find_all_files(dir, mask, false);
if (!found_prefix_files) error = GB_get_error();
}
free(mask);
if (!error) {
mask = GBS_global_string_copy("*.%s", name_postfix);
if (overlay_postfix) {
found_postfix_files = GB_find_all_files(dir, mask, false);
if (!found_postfix_files) error = GB_get_error();
}
free(mask);
}
}
if (!error) {
if (found_prefix_files) {
if (found_postfix_files) {
found_files = GBS_global_string_copy("%s*%s", found_prefix_files, found_postfix_files);
}
else { // only found_prefix_files
found_files = found_prefix_files;
found_prefix_files = 0;
}
}
else {
found_files = found_postfix_files;
found_postfix_files = 0;
}
}
free(found_postfix_files);
free(found_prefix_files);
}
free(name_postfix);
free(name_prefix);
free(dir);
return found_files;
}
enum PlotType {
PT_GC_RATIO,
PT_GA_RATIO,
PT_RATE,
PT_TT_RATIO,
PT_MOST_FREQUENT_BASE,
PT_SECOND_FREQUENT_BASE,
PT_THIRD_FREQUENT_BASE,
PT_LEAST_FREQUENT_BASE,
PT_BASE_A,
PT_BASE_C,
PT_BASE_G,
PT_BASE_TU,
PT_HELIX,
PT_PLOT_TYPES,
PT_UNKNOWN
};
static const char *plotTypeName[PT_PLOT_TYPES] = {
"gc_gnu",
"ga_gnu",
"rate_gnu",
"tt_gnu",
"f1_gnu",
"f2_gnu",
"f3_gnu",
"f4_gnu",
"a_gnu",
"c_gnu",
"g_gnu",
"tu_gnu",
"helix_gnu"
};
static const char *plotTypeDescription[PT_PLOT_TYPES] = {
"G+C ratio",
"G+A ratio",
"Rate",
"TT Ratio",
"Most frequent base",
"2nd frequent base",
"3rd frequent base",
"Least frequent base",
"A ratio",
"C ratio",
"G ratio",
"T/U ratio",
"Helix"
};
static PlotType string2PlotType(const char *type) {
for (int pt = 0; pt<PT_PLOT_TYPES; ++pt) {
if (strcmp(type, plotTypeName[pt]) == 0) {
return PlotType(pt);
}
}
return PT_UNKNOWN;
}
static const char *makeTitle(const char *fname) {
const char *rslash = strrchr(fname, '/');
if (rslash) rslash++;
else rslash = fname;
char *name = strdup(rslash);
char *rdot = strrchr(name, '.');
PlotType pt = PT_UNKNOWN;
if (rdot) pt = string2PlotType(rdot+1);
static char *title = 0;
freenull(title);
if (pt == PT_UNKNOWN) {
title = GBS_global_string_copy("%s (unknown type)", name);
}
else {
rdot[0] = 0;
title = GBS_global_string_copy("%s (%s)", name, plotTypeDescription[pt]);
}
free(name);
return title;
}
// -------------------
// SortedFreq
class SortedFreq : virtual Noncopyable {
float *freq[4];
public:
SortedFreq(const ColumnStat *column_stat);
~SortedFreq();
float get(PlotType plot_type, size_t pos) const {
float f;
switch (plot_type) {
case PT_MOST_FREQUENT_BASE: f = freq[0][pos]; break;
case PT_SECOND_FREQUENT_BASE: f = freq[1][pos]; break;
case PT_THIRD_FREQUENT_BASE: f = freq[2][pos]; break;
case PT_LEAST_FREQUENT_BASE: f = freq[3][pos]; break;
default: nt_assert(0); f = 0; break;
}
return f;
}
};
SortedFreq::SortedFreq(const ColumnStat *column_stat) {
size_t len = column_stat->get_length();
for (int i = 0; i<4; ++i) { // 4 best frequencies
freq[i] = new float[len];
for (size_t p = 0; p<len; ++p) freq[i][p] = 0.0; // clear
}
for (unsigned int c = 0; c<256; ++c) { // all character stats
const float *cfreq = column_stat->get_frequencies((unsigned char)c);
if (cfreq) {
for (size_t p = 0; p<len; ++p) { // all positions
if (freq[3][p] < cfreq[p]) {
freq[3][p] = cfreq[p]; // found higher freq
for (int i = 3; i > 0; --i) { // bubble upwards to sort
if (freq[i-1][p] >= freq[i][p]) break; // sorted!
float f = freq[i][p];
freq[i][p] = freq[i-1][p];
freq[i-1][p] = f;
}
}
}
}
}
#if defined(DEBUG)
for (size_t p = 0; p<len; ++p) { // all positions
nt_assert(freq[0][p] >= freq[1][p]);
nt_assert(freq[1][p] >= freq[2][p]);
nt_assert(freq[2][p] >= freq[3][p]);
}
#endif // DEBUG
}
SortedFreq::~SortedFreq() {
for (int i = 0; i<4; ++i) delete [] freq[i];
}
class Smoother : virtual Noncopyable {
double *value;
size_t *index;
size_t size;
size_t halfsize;
size_t next;
size_t added;
double sum;
size_t wrap(size_t idx) { return idx >= size ? idx-size : idx; }
public:
Smoother(size_t smooth_range)
: size(smooth_range),
halfsize(size/2),
next(0),
added(0),
sum(0.0)
{
nt_assert(size>0);
value = new double[size];
index = new size_t[size];
for (size_t i = 0; i<size; ++i) value[i] = 0.0;
}
~Smoother() {
delete [] value;
delete [] index;
}
void print(FILE *out, size_t i, double v) {
sum = sum+v-value[next];
index[next] = i;
value[next] = v;
next = wrap(next+1);
if (added<size) ++added;
if (added == size) {
size_t printNext = wrap(next+halfsize);
fprintf(out, "%zu %f\n", index[printNext], sum/size);
}
}
};
static void colstat_2_gnuplot_cb(AW_window *aww, AW_CL cl_column_stat, AW_CL cl_mode) {
// cl_mode = 0 -> write file
// cl_mode = 1 -> write file and run gnuplot
// cl_mode = 2 -> delete all files with same prefix
GB_transaction ta(GLOBAL.gb_main);
ColumnStat *column_stat = (ColumnStat *)cl_column_stat;
GB_ERROR error = 0;
int mode = int(cl_mode);
if (mode != 2) {
char *filterstring = aww->get_root()->awar(AWAR_CS2GP_FILTER_FILTER)->read_string();
char *alignment_name = aww->get_root()->awar(AWAR_CS2GP_FILTER_ALIGNMENT)->read_string();
long alignment_length = GBT_get_alignment_len(GLOBAL.gb_main, alignment_name);
if (alignment_length<0) {
GB_clear_error();
error = "Please select a valid alignment";
}
else {
AP_filter filter(filterstring, "0", alignment_length);
free(alignment_name);
free(filterstring);
error = column_stat->calculate(&filter);
if (!error && !column_stat->get_length()) error = "Please select column statistic";
}
}
if (!error) {
char *fname = aww->get_root()->awar(AWAR_CS2GP_FILENAME)->read_string();
if (!strchr(fname, '/')) freeset(fname, GBS_global_string_copy("./%s", fname));
if (strlen(fname) < 1) error = "Please enter file name";
if (mode == 2) { // delete overlay files
if (!error) {
char *found_files = get_overlay_files(aww->get_root(), fname, error);
if (found_files) {
for (char *name = strtok(found_files, "*"); name; name = strtok(0, "*")) {
printf("Deleting gnuplot file '%s'\n", name);
if (unlink(name) != 0) printf("Can't delete '%s'\n", name);
}
free(found_files);
aww->get_root()->awar(AWAR_CS2GP_DIRECTORY)->touch(); // reload file selection box
}
}
}
else {
FILE *out = 0;
if (!error) {
out = fopen(fname, "w");
if (!out) error = GB_export_errorf("Cannot write to file '%s'", fname);
}
nt_assert(out || error);
if (!error) {
char *type = aww->get_root()->awar(AWAR_CS2GP_SUFFIX)->read_string();
long smoothSize = aww->get_root()->awar(AWAR_CS2GP_SMOOTH_VALUES)->read_int(); // 1 = discrete values
size_t columns = column_stat->get_length();
enum {
STAT_AMOUNT,
STAT_SIMPLE_FLOAT,
STAT_SIMPLE_BOOL,
STAT_SORT,
STAT_UNKNOWN
} stat_type = STAT_UNKNOWN;
union {
struct {
const float *A;
const float *C;
const float *G;
const float *TU;
} amount; // STAT_AMOUNT
const float *floatVals; // STAT_SIMPLE_FLOAT
const bool *boolVals; // STAT_SIMPLE_BOOL
SortedFreq *sorted; // STAT_SORT
} data;
PlotType plot_type = string2PlotType(type);
switch (plot_type) {
case PT_GC_RATIO:
case PT_GA_RATIO:
case PT_BASE_A:
case PT_BASE_C:
case PT_BASE_G:
case PT_BASE_TU: {
stat_type = STAT_AMOUNT;
data.amount.A = column_stat->get_frequencies('A');
data.amount.C = column_stat->get_frequencies('C');
data.amount.G = column_stat->get_frequencies('G');
data.amount.TU = column_stat->get_frequencies('U');
break;
}
case PT_RATE:
stat_type = STAT_SIMPLE_FLOAT;
data.floatVals = column_stat->get_rates();
break;
case PT_TT_RATIO:
stat_type = STAT_SIMPLE_FLOAT;
data.floatVals = column_stat->get_ttratio();
break;
case PT_HELIX: {
stat_type = STAT_SIMPLE_BOOL;
data.boolVals = column_stat->get_is_helix();
break;
}
case PT_MOST_FREQUENT_BASE:
case PT_SECOND_FREQUENT_BASE:
case PT_THIRD_FREQUENT_BASE:
case PT_LEAST_FREQUENT_BASE: {
stat_type = STAT_SORT;
data.sorted = new SortedFreq(column_stat);
break;
}
case PT_PLOT_TYPES:
case PT_UNKNOWN:
error = "Please select what to plot";
break;
}
const GB_UINT4 *weights = column_stat->get_weights();
if (!error) {
Smoother smoother(smoothSize);
for (size_t j=0; j<columns; ++j) {
if (!weights[j]) continue;
double val = 0;
switch (stat_type) {
case STAT_AMOUNT: {
float A = data.amount.A[j];
float C = data.amount.C[j];
float G = data.amount.G[j];
float TU = data.amount.TU[j];
float amount = A+C+G+TU;
switch (plot_type) {
case PT_GC_RATIO: val = (G+C)/amount; break;
case PT_GA_RATIO: val = (G+A)/amount; break;
case PT_BASE_A: val = A/amount; break;
case PT_BASE_C: val = C/amount; break;
case PT_BASE_G: val = G/amount; break;
case PT_BASE_TU: val = TU/amount; break;
default: nt_assert(0); break;
}
break;
}
case STAT_SIMPLE_FLOAT: val = data.floatVals[j]; break;
case STAT_SIMPLE_BOOL: val = data.boolVals[j]; break;
case STAT_SORT: val = data.sorted->get(plot_type, j); break;
case STAT_UNKNOWN: nt_assert(0); break;
}
smoother.print(out, j, val);
}
}
if (stat_type == STAT_SORT) delete data.sorted;
free(type);
}
if (out) {
fclose(out);
out = NULL;
}
if (!error) {
aww->get_root()->awar(AWAR_CS2GP_DIRECTORY)->touch(); // reload file selection box
if (mode == 1) { // run gnuplot ?
char *command_file;
char *command_name = GB_unique_filename("arb", "gnuplot");
out = GB_fopen_tempfile(command_name, "wt", &command_file);
if (!out) error = GB_await_error();
else {
char *smooth = aww->get_root()->awar(AWAR_CS2GP_SMOOTH_GNUPLOT)->read_string();
char *found_files = get_overlay_files(aww->get_root(), fname, error);
fprintf(out, "set samples 1000\n");
bool plotted = false; // set to true after first 'plot' command (other plots use 'replot')
const char *plot_command[] = { "plot", "replot" };
if (found_files) {
for (char *name = strtok(found_files, "*"); name; name = strtok(0, "*")) {
if (strcmp(name, fname) != 0) { // latest data file is done below
fprintf(out, "%s \"%s\" %s title \"%s\"\n", plot_command[int(plotted)], name, smooth, makeTitle(name));
plotted = true;
}
}
free(found_files);
}
fprintf(out, "%s \"%s\" %s title \"%s\"\n", plot_command[int(plotted)], fname, smooth, makeTitle(fname));
fprintf(out, "pause mouse any \"Any key or button will terminate gnuplot\"\n");
fclose(out);
out = 0;
if (mode == 1) {
char *script = GBS_global_string_copy("gnuplot %s && rm -f %s", command_file, command_file);
GB_xcmd(script, true, true);
free(script);
}
else {
nt_assert(mode == 2);
GB_unlink_or_warn(command_file, &error);
}
free(smooth);
}
free(command_file);
free(command_name);
}
}
}
free(fname);
}
if (error) aw_message(error);
}
static void colstat_ali_changed_cb(AW_root *root) {
if (GLOBAL.gb_main) {
long smooth_max = GBT_get_alignment_len(GLOBAL.gb_main, root->awar(AWAR_CS2GP_FILTER_ALIGNMENT)->read_char_pntr());
if (smooth_max<0) { // ali not found
GB_clear_error();
smooth_max = 1; // min=max -> do not limit
}
root->awar(AWAR_CS2GP_SMOOTH_VALUES)->set_minmax(1, smooth_max);
}
}
AW_window *NT_create_colstat_2_gnuplot_window(AW_root *root) {
GB_transaction ta(GLOBAL.gb_main);
AW_awar *awar_default_alignment = root->awar_string(AWAR_DEFAULT_ALIGNMENT, "", GLOBAL.gb_main);
ColumnStat *column_stat = new ColumnStat(GLOBAL.gb_main, root, AWAR_CS2GP_NAME, awar_default_alignment);
AW_window_simple *aws = new AW_window_simple;
aws->init(root, "EXPORT_CSP_TO_GNUPLOT", "Export Column statistic to GnuPlot");
aws->load_xfig("cpro/csp_2_gnuplot.fig");
root->awar_int(AWAR_CS2GP_SMOOTH_VALUES, 1);
root->awar_int(AWAR_CS2GP_GNUPLOT_OVERLAY_POSTFIX);
root->awar_int(AWAR_CS2GP_GNUPLOT_OVERLAY_PREFIX);
root->awar_string(AWAR_CS2GP_SMOOTH_GNUPLOT);
root->awar_string(AWAR_CS2GP_FILTER_NAME);
root->awar_string(AWAR_CS2GP_FILTER_FILTER);
AW_awar *awar_ali = root->awar_string(AWAR_CS2GP_FILTER_ALIGNMENT);
awar_ali->map(AWAR_DEFAULT_ALIGNMENT); // use current alignment for filter
awar_ali->add_callback(colstat_ali_changed_cb);
awar_ali->touch();
AW_create_fileselection_awars(root, AWAR_CS2GP, "", ".gc_gnu", "noname.gc_gnu");
aws->at("close"); aws->callback((AW_CB0)AW_POPDOWN);
aws->create_button("CLOSE", "CLOSE", "C");
aws->at("help"); aws->callback(makeHelpCallback("csp_2_gnuplot.hlp"));
aws->create_button("HELP", "HELP", "H");
AW_create_standard_fileselection(aws, AWAR_CS2GP);
aws->at("csp");
COLSTAT_create_selection_list(aws, column_stat);
aws->at("what");
AW_selection_list *plotTypeList = aws->create_selection_list(AWAR_CS2GP_SUFFIX, true);
for (int pt = 0; pt<PT_PLOT_TYPES; ++pt) {
plotTypeList->insert(plotTypeDescription[pt], plotTypeName[pt]);
}
plotTypeList->insert_default("<select one>", "");
plotTypeList->update();
adfiltercbstruct *filter = awt_create_select_filter(root, GLOBAL.gb_main, AWAR_CS2GP_FILTER_NAME);
aws->at("ap_filter");
aws->callback(makeCreateWindowCallback(awt_create_select_filter_win, filter));
aws->create_button("SELECT_FILTER", AWAR_CS2GP_FILTER_NAME);
aws->at("smooth");
aws->create_input_field(AWAR_CS2GP_SMOOTH_VALUES, 8);
aws->at("smooth2");
aws->create_toggle_field(AWAR_CS2GP_SMOOTH_GNUPLOT, 1);
aws->insert_default_toggle("None", "N", "");
aws->insert_toggle("Unique", "U", "smooth unique");
aws->insert_toggle("CSpline", "S", "smooth cspline");
aws->insert_toggle("Bezier", "B", "smooth bezier");
aws->update_toggle_field();
aws->auto_space(10, 10);
aws->button_length(13);
aws->at("save");
aws->callback(colstat_2_gnuplot_cb, (AW_CL)column_stat, (AW_CL)0);
aws->create_button("SAVE", "Save");
aws->highlight();
aws->callback(colstat_2_gnuplot_cb, (AW_CL)column_stat, (AW_CL)1);
aws->create_button("SAVE_AND_VIEW", "Save & View");
aws->at("overlay1");
aws->label("Overlay statistics with same prefix?");
aws->create_toggle(AWAR_CS2GP_GNUPLOT_OVERLAY_PREFIX);
aws->at("overlay2");
aws->label("Overlay statistics with same postfix?");
aws->create_toggle(AWAR_CS2GP_GNUPLOT_OVERLAY_POSTFIX);
aws->at("del_overlays");
aws->callback(colstat_2_gnuplot_cb, (AW_CL)column_stat, (AW_CL)2);
aws->create_autosize_button("DEL_OVERLAYS", "Delete currently overlayed files", "D", 2);
return (AW_window *)aws;
}
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