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# ==================================================================== #
# #
# File : GI.pm #
# Purpose : Genome import library #
# #
# #
# Coded by Ralf Westram (coder@reallysoft.de) in December 2003 #
# Copyright Department of Microbiology (Technical University Munich) #
# #
# Visit our web site at: http://www.arb-home.de/ #
# #
# #
# ==================================================================== #
package GI;
use strict;
use warnings;
use ARB;
my $gb_main;
my $columns;
sub connectDB() {
print "Connecting to running ARB database\n";
$gb_main = ARB::open(":","r");
if (! $gb_main ) {
my $error = ARB::await_error();
print $error."\n";
exit 0;
}
ARB::begin_transaction($gb_main);
}
sub disconnectDB() {
ARB::commit_transaction($gb_main);
ARB::close($gb_main);
}
sub findCurrentGenome() {
my $organism = BIO::read_string($gb_main, 'tmp/focus/organism_name'); # aka AWAR_ORGANISM_NAME
if ($organism eq '') {
error("You have to select the target organism in ARB!");
}
my $gb_orga = BIO::find_organism($gb_main,$organism);
if (!$gb_orga) { error("'$organism' is not a organism"); }
return ($gb_orga,$organism);
}
sub with_marked_genes($\&) {
my ($gb_orga,$fun_r) = @_;
my $gb_gene = BIO::first_marked_gene($gb_orga);
while ($gb_gene) {
&$fun_r($gb_gene);
$gb_gene = BIO::next_marked_gene($gb_gene);
}
}
sub with_all_genes($\&) {
my ($gb_orga,$fun_r) = @_;
my $gb_gene = BIO::first_gene($gb_orga);
while ($gb_gene) {
&$fun_r($gb_gene);
$gb_gene = BIO::next_gene($gb_gene);
}
}
sub with_marked_genomes(\&) {
my ($fun_r) = @_;
my $gb_orga = BIO::first_marked_organism($gb_main);
while ($gb_orga) {
&$fun_r($gb_orga);
$gb_orga = BIO::next_marked_organism($gb_orga);
}
}
sub with_all_genomes(\&) {
my ($fun_r) = @_;
my $gb_orga = BIO::first_organism($gb_main);
while ($gb_orga) {
&$fun_r($gb_orga);
$gb_orga = BIO::next_organism($gb_orga);
}
}
sub unmark_gene($) {
my ($gb_gene) = @_;
ARB::write_flag($gb_gene, 0); # unmark
}
sub unmarkGenesOfGenome($) {
my ($gb_genome) = @_;
with_marked_genes($gb_genome, &unmark_gene);
}
sub findORF($$$$$) {
my ($gb_gene_data,$genome_name,$orf,$create,$verbose) = @_;
my $error;
my $gb_orf;
my $gb_locus_tag = ARB::find_string($gb_gene_data, "locus_tag", $orf, 1, "grandchild");
if (!$gb_locus_tag) {
if ($create==0) {
$error = "no gene with locus_tag '$orf' found for organism '$genome_name'";
}
else {
my $gb_genome = ARB::get_father($gb_gene_data);
$gb_orf = BIO::create_nonexisting_gene($gb_genome, $orf);
if (!$gb_orf) {
my $reason = ARB::await_error();
$error = "cannot create gene '$orf' ($reason)";
}
else {
my $gb_locus_tag = ARB::search($gb_orf, "locus_tag", "STRING");
if (!$gb_locus_tag) {
my $reason = ARB::await_error();
$error = "cannot create field 'locus_tag' ($reason)";
}
else {
$error = ARB::write_string($gb_locus_tag, $orf);
if ($error) {
$error = "Couldn't write to 'locus_tag' ($error)";
}
}
}
if (!$error and $verbose==1) { print "Created new gene '$orf'\n"; }
}
}
else {
$gb_orf = ARB::get_father($gb_locus_tag);
}
if (!$gb_orf) { if (!$error) { die "Internal error"; }}
return ($gb_orf,$error);
}
sub write_entry($$$$$$) {
my ($gb_container, $field_name, $field_type, $field_content, $overwrite, $verbose) = @_;
my $gb_field = ARB::search($gb_container, $field_name, "NONE");
my $error;
if (!$gb_field) {
$gb_field = ARB::search($gb_container, $field_name, $field_type);
if (!$gb_field) {
my $reason = ARB::await_error();
$error = "Can't create '$field_name' ($reason)";
}
}
else {
if ($overwrite==0) {
$error = "Field '$field_name' already exists";
}
}
if (!$error) {
if (!$gb_field) { die "internal error"; }
$error = ARB::write_as_string($gb_field, $field_content);
if ($error) { $error = "Cannot write to '$field_name' ($error)"; }
}
return $error;
}
# --------------------------------------------------------------------------------
sub show_csv_info() {
print " CSV may be saved with Excel and StarCalc. It simply is a\n".
" comma separated list with strings quoted in \"\". The first line\n".
" contains the column titles.\n";
}
sub message($) {
my ($msg) = @_;
BIO::message($gb_main, $msg);
print "$msg\n";
}
sub error($) {
my ($msg) = @_;
$msg = "Error: ".$msg;
ARB::commit_transaction($gb_main); # this undoes all changes made by this script
# ARB::abort_transaction($gb_main); # this undoes all changes made by this script
ARB::begin_transaction($gb_main);
BIO::message($gb_main, $msg);
BIO::message($gb_main, "Script aborted!");
ARB::commit_transaction($gb_main);
die $msg."\n";
}
# --------------------------------------------------------------------------------
sub define_tokenizer_columns($) {
($columns) = @_;
}
sub tokenize_columns($$) {
my ($line,$errline) = @_;
chomp $line;
$line .= ',';
my @array = ();
while (not $line =~ /^[ ]*$/ig) {
if ($line =~ /^[ ]*\"([^\"]*)\"[ ]*,/ig) {
my $content = $1;
$line = $';
$content =~ s/^[ ]*//ig;
$content =~ s/[ ]*$//ig;
push @array, $content;
}
elsif ($line =~ /[ ]*([0-9]+)[ ]*,/ig) {
push @array, $1;
$line = $';
}
else {
error("cannot parse line $errline (at '$line')");
}
}
my $cols = @array;
if ($cols != $columns) {
error("expected $columns columns (found $cols) in line $errline");
}
return @array;
}
# --------------------------------------------------------------------------------
1; # result of module initialization
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