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# Program: restrict
# Rundate: Wed 18 Feb 2009 11:01:39
# Commandline: restrict
# [-sequence] /Users/tjc/foo.embl
# -auto
# -limit y
# -enzymes HincII,hinfI,ppiI,hindiii
# -outfile /private/tmp/circular_genome57650.txt
# Report_format: table
# Report_file: /private/tmp/circular_genome57650.txt
########################################
#=======================================
#
# Sequence: from: 1 to: 41243
# HitCount: 158
#
# Minimum cuts per enzyme: 1
# Maximum cuts per enzyme: 2000000000
# Minimum length of recognition site: 4
# Blunt ends allowed
# Sticky ends allowed
# DNA is linear
# Ambiguities allowed
#
#=======================================
Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev
81 86 + HindIII AAGCTT 81 85 . .
140 144 + HinfI GANTC 140 143 . .
40956 40960 + HinfI GANTC 40956 40959 . .
41036 41041 + HindII GTYRAC 41038 41038 . .
#---------------------------------------
#---------------------------------------
#---------------------------------------
# Total_sequences: 1
# Total_hitcount: 158
#---------------------------------------
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