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/**********************************************************************
* file: genbank.cc
* licence: Artistic Licence, see file LICENCE.TXT or
* http://www.opensource.org/licenses/artistic-license.php
* descr.: sequence and gene struture input from file
* authors: Mario Stanke, mario@gobics.de, Stafilarakis
*
* date | author | changes
* ---------|---------------|------------------------------------------
* 03.01.07 | Mario Stanke | fixed bug with clength (was 3 too long for every exon except the last)
* 05.04.07 | Mario Stanke | Do not read out incomplete mRNA if it does not have a
* | | matching but an overlapping CDS. Usefull for training with 3'incompete genes.
**********************************************************************/
#include "genbank.hh"
#include "fasta.hh"
// project includes
#include "properties.hh"
#include "gene.hh"
#include "projectio.hh" // for goto_line_after
// standard C/C++ includes
#include <iostream>
#ifdef ZIPINPUT
using boost::iostreams::filtering_istream;
using boost::iostreams::gzip_decompressor;
#endif
GBProcessor::GBProcessor(string filename) :
gbs(filename)
{
try{
gbVerbosity = Properties::getIntProperty("/genbank/verbosity");
} catch (...){
gbVerbosity = 3;
}
}
GBPositions* GBProcessor::nextPosition( ){
return gbs.nextData( );
}
AnnoSequence* GBProcessor::getAnnoSequence( GBPositions* pos ){
if( !pos )
return (AnnoSequence*) 0;
bool stopCodonExcludedFromCDS = false;
try {
stopCodonExcludedFromCDS = Properties::getBoolProperty("stopCodonExcludedFromCDS");
} catch (ProjectError e) {
stopCodonExcludedFromCDS = false;
}
bool withUTR;
try {
string utr = Properties::getProperty("UTR");
withUTR = (utr == "both" || utr == "1" || utr == "on" || utr == "5");
} catch (ProjectError e) {
withUTR = false;
}
AnnoSequence *annoseq = new AnnoSequence();
if (!annoseq)
return NULL;
annoseq->anno = new Annotation;
annoseq->sequence = getSequence( *pos );
annoseq->length = pos->seqlength;
annoseq->bc.addSequence(annoseq->sequence, strlen(annoseq->sequence) );
char* idtmp = strstr(pos->buffer, "LOCUS");
if (idtmp == NULL) {
annoseq->seqname = newstrcpy("unknown");
} else {
annoseq->seqname = new char[100];
idtmp += strlen("LOCUS");
while( isspace(*idtmp) )
idtmp++;
int i;
for( i = 0; i < 99 && !isspace(*idtmp); i++, idtmp++ ){
annoseq->seqname[i] = *idtmp;
}
annoseq->seqname[i] = '\0';
}
int numGenes = pos->CDS.size();
if (numGenes == 0 && gbVerbosity > 0) {
cerr << "No 'CDS' found in sequence " << annoseq->seqname << endl;
}
/*
* make a list of genes from the data in pos
*
*/
list<Gene*> allgenes, *allgenesSorted;
int curGeneNr = 1;
list<GBFeature>::iterator fit, mRNAit;
int lastgeneend=-1;
Gene *gene;
pos->CDS.sort();
pos->mRNA.sort();
// make genes from the CDS first
for (fit = pos->CDS.begin(); fit != pos->CDS.end(); fit++) {
bool cont=true;
if (fit->begin <= lastgeneend) {
cerr << "CDS overlaps with other CDS (" << (fit->begin+1) << " <= " << (lastgeneend+1) << ") in sequence " << annoseq->seqname
<< ". Ignoring this gene." << endl;
cont = false;
}
if (cont && gbVerbosity > 0 && (!fit->complete_l || !fit->complete_r)){
cerr << "Coding sequence incomplete in sequence " << annoseq->seqname << "." << endl;
//cont = false;
}
if (cont) {
gene = new Gene;
// set the id, geneid, seqname and source
gene->id = "1";
if (fit->geneid != "") {
gene->geneid = fit->geneid;
} else {
gene->geneid = string(annoseq->seqname) + "-" + itoa(curGeneNr);
// char *temp = new char[strlen(annoseq->seqname)+6];
// sprintf(temp, "%s-%d",annoseq->seqname, curGeneNr );
// gene->geneid = temp;
}
gene->seqname = annoseq->seqname;
gene->source = "database";
gene->length = pos->seqlength;
gene->clength = 0;
gene->codingstart = gene->codingend = -1;
gene->strand = fit->strand;
gene->bc = annoseq->bc;
gene->exons = fit->ranges;
/*
* construct the coding exons and introns
*/
State *exon_old=NULL, *intron_old=NULL, *intron=NULL, *st = gene->exons;
while( st ){
gene->clength += st->length();
if (st == gene->exons)
if (st->next == NULL)
st->type = (fit->strand == plusstrand)? singleG : rsingleG;
else
if (fit->strand == plusstrand)
st->type = initialExon(gene->clength);
else
st->type = rterminalExon(2 - gene->clength);
else if (st->next == NULL)
if (fit->strand == plusstrand)
st->type = terminal;
else
st->type = rinitial;
else
if (fit->strand == plusstrand)
st->type = internalExon(gene->clength);
else
st->type = rinternalExon(2 - gene->clength);
if (stopCodonExcludedFromCDS) {
if (st->type == singleG || st->type == terminal){
gene->clength += 3;
st->end += 3;
}
if (st->type == rsingleG || isRTerminalExon(st->type)){
gene->clength += 3;
st->begin -= 3;
}
if (st->begin < 0 || st->end >= annoseq->length)
throw GBError("Stop codon out of sequence bounds. Ignoring sequence.");
}
if (gene->codingstart < 0 || gene->codingstart > st->begin)
gene->codingstart = st->begin;
if (gene->codingend < st->end)
gene->codingend = st->end;
if( exon_old ){
intron = new State();
intron->begin = exon_old->end+1;
intron->end = st->begin-1;
if (intron->begin > intron->end){
cerr << "Error: In sequence " << annoseq->seqname << ": One CDS exon does not begin properly after the previous CDS exon."
<< exon_old->end << " >= " << st->begin << endl;
throw GBError("Intron has non-positive length.");
}
intron->type = intron_type;
intron->next = 0;
if( !gene->introns )
gene->introns = intron;
if( intron_old )
intron_old->next = intron;
intron_old = intron;
}
exon_old=st;
st = st->next;
}
if (withUTR){
/*
* look if there is any fitting mRNA: positions compatible (and same geneid)
* take the first fitting but delete all fitting mRNAs
* construct the utr
*/
bool mrnafound = false;
mRNAit = pos->mRNA.begin();
GBFeature mrnaFeature;
while(mRNAit != pos->mRNA.end()){
if (fit->matches(*mRNAit)){
if (!mrnafound)
mrnaFeature = *mRNAit;
mrnafound = true;
mRNAit = pos->mRNA.erase(mRNAit);
} else {
mRNAit++;
}
}
if (mrnafound){
// construct the utr
gene->addUTR(mrnaFeature.ranges, mrnaFeature.complete_l, mrnaFeature.complete_r);
}
}
// gene->computeBC(annoseq->sequence);
allgenes.push_back(gene);
lastgeneend = fit->end;
curGeneNr++;
}
}
if (withUTR){
/*
* make incomplete genes from the remaining mRNAs
*/
for (mRNAit = pos->mRNA.begin(); mRNAit != pos->mRNA.end(); mRNAit++) {
gene = new Gene;
// set the id, geneid, seqname and source
gene->id = "1";
if (mRNAit->geneid != "") {
gene->geneid = mRNAit->geneid;
} else {
gene->geneid = string(annoseq->seqname) + "-" + itoa(curGeneNr);
// char *temp = new char[strlen(annoseq->seqname)+6];
// sprintf(temp, "%s-%d",annoseq->seqname, curGeneNr );
// gene->geneid = temp;
}
gene->seqname = annoseq->seqname;
gene->source = "database";
gene->length = pos->seqlength;
gene->clength = 0;
gene->codingstart = gene->codingend = -1;
gene->strand = mRNAit->strand;
gene->bc = annoseq->bc;
gene->exons = NULL;
// add the UTR
gene->addUTR(mRNAit->ranges, mRNAit->complete_l, mRNAit->complete_r);
// check whether it overlaps a CDS
// If yes, don't use it.
bool overlapsCDS = false;
for (list<Gene*>::iterator git = allgenes.begin(); git != allgenes.end(); git++)
if ((gene->transstart <= (*git)->codingstart && gene->transend >= (*git)->codingstart) ||
(gene->transstart >= (*git)->codingstart && gene->transstart <= (*git)->codingend)){
overlapsCDS = true;
}
if ((gene->utr5exons || gene->utr3exons) && !overlapsCDS){
// gene->computeBC(annoseq->sequence);
allgenes.push_back(gene);
curGeneNr++;
}
}
}
if (allgenes.empty()) {
cerr << "No correct 'CDS'";
if (withUTR)
cerr << "or 'mRNA'";
cerr << " found in sequence " << annoseq->seqname << endl;
}
allgenesSorted = sortGenePtrList(allgenes);
for (list<Gene*>::iterator git = allgenesSorted->begin(); git != allgenesSorted->end(); git++)
annoseq->anno->appendGene(*git);
//annoseq->printGFF();
return annoseq;
}
/*
* GBProcessor::getAnnoSequenceList()
*
* gets the list of all annotated sequences from
*
*/
AnnoSequence* GBProcessor::getAnnoSequenceList(){
AnnoSequence *first = NULL, *annoseq, *last = NULL;
bool notend = true;
int annocount = 0;
while (notend){
try {
annoseq = getAnnoSequence(nextPosition( ));
if ((notend = (annoseq != NULL))){
annoseq->next = NULL;
if (last)
last->next = annoseq;
else
first = annoseq;
last = annoseq;
annocount++;
}
} catch (GBError e) {
cerr << "GBProcessor::getGeneList(): " << e.getMessage() << endl;
cerr << "Encountered error after reading " << annocount << " annotations." << endl;
}
}
if (annocount==0)
throw ProjectError("No genbank sequences found.");
if (gbVerbosity)
cout << "# Read in " << annocount << " genbank sequences." << endl;
return first;
}
AnnoSequence* GBProcessor::getSequenceList(){
AnnoSequence *seqlist = NULL, *seq, *last = NULL;
while((seq = gbs.getNextFASTASequence())){
seq->next = NULL;
if (last)
last->next = seq;
else
seqlist = seq;
last = seq;
};
gbs.clear();
if (seqlist == NULL)
throw ProjectError("No sequences found.");
return seqlist;
}
char* GBProcessor::getSequence( GBPositions& pos ){
char* seq = new char[pos.seqlength+1];
if( seq ){
char* seqp = seq;
istringstream isstrm( pos.seqbegin );
char c;
int i;
int count = 0;
isstrm.getline(seq, pos.seqlength+1);
while( isstrm ){
isstrm >> i; // Zahl zu Begin der Zeile lesen!
while( isstrm.get( c ) && c != '\n' )
if( isalpha( c ) ){
if (count++ < pos.seqlength)
*seqp++ = c; // tolower now postponed to after softmasking detection
else {
char buffer [140];
sprintf (buffer, "Sequence was longer than the expected %d bp. Please compare the line 'source 1..X' with the actual sequence length.", pos.seqlength);
throw GBError(buffer);
}
}
}
if (count < pos.seqlength) {
cerr << "Warning: Sequence was shorter than expected frome the source line of the Genbank entry ( expected " << pos.seqlength
<< " bp). Please compare the line 'source 1..X' with the actual sequence length (" << count << ")." << endl;
pos.seqlength = count;
}
seq[pos.seqlength]='\0';
}
return seq;
}
GBFeature::GBFeature(const char *pos){
begin = end = -1;
ranges= NULL;
complete_r=complete_l=true;
istringstream isstrm( pos );
char c;
char tmp[GBMAXLINELEN] = { '\0' }; // need more than 4200 chars for human muscle gene TTN
char* join = (char*)0;
int i = 0;
isstrm >> fkey >> ws; // read 'CDS','mRNA' or signal FKEY
c = isstrm.peek( );
if (c == 'c') { // "complement"
strand = minusstrand;
while( isstrm.get( c ) && c != '(' && c != '\n')
;
} else
strand = plusstrand;
c = isstrm.peek( );
if( isalpha( c ) ){
while( isstrm.get( c ) && c != '(' && c != '\n')
;
if ( c == '\n') {
cerr << "Feature key = " << fkey << ", c = " << c << endl;
throw GBError( "GBProcessor::getJoin( ): failed!!!" );
}
while( isstrm.get( c ) && c != ')' )
if( !isspace( c ) ) {
if (isdigit(c) || c =='.' || c == ',' || c=='<' || c=='>' )
tmp[i++] = c;
else {
cerr << fkey << " contains character " << c << endl;
throw GBError( "GBProcessor::getJoin( ): failed!!!" );
}
}
while( isstrm.get( c ) && c != '\n' )
; // read up to the end of the line
} else if( isdigit( c ) || c == '<'){
while( isstrm.get( c ) && c != ')' && c != '\n' )
tmp[i++] = c;
while (isstrm && (c != '\n')) // read until end of line
isstrm.get( c );
} else {
cerr << "Feature key = " << fkey << ", c = " << c << endl;
throw GBError( "GBProcessor::getJoin( ): failed!!!" );
}
join = newstrcpy(tmp,i);
// try to determine the name of the gene
char *genename;
char buf[GBMAXLINELEN];
bool searchFinished = false;
char *gtag;
while( isstrm && !searchFinished){
isstrm.getline( buf, GBMAXLINELEN-1 );
if (strncmp(buf, " ", 21) != 0) { // name of the gene not found
geneid="";
searchFinished = true;
}
else {
gtag = strstr(buf, "/gene=");
if (gtag){// found the name of the gene in the annotation
int cplen = strcspn(gtag + 7, "\"");
genename = newstrcpy(gtag + 7, cplen);
geneid = genename;
searchFinished = true;
}
}
}
/*
* Now construct the ranges state sequence
*/
try {
State *exon = 0, *exon_old = 0;
istringstream joinstrm( join );
while( joinstrm ){
char c;
int ebegin=-1, eend=-1;
exon = new State();
c = joinstrm.peek( );
if (c=='<'){
complete_l = false;
joinstrm.get( c );
}
joinstrm >> ebegin >> c >> c;
c = joinstrm.peek( );
if (c=='>'){
complete_r = false;
joinstrm.get( c );
}
joinstrm >> eend >> c; // format: 'Numer..Number,'
if (ebegin < 1 || eend < 1)
throw ProjectError(string("Wrong format for coordinates: ") + join);
if (ebegin > eend)
throw ProjectError(string("Feature begins after it ends: ") + join);
exon->begin = ebegin - 1; // correct for the fact that indices
exon->end = eend - 1; // start with 0 in the c++ sequence
exon->next = 0;
if( !ranges )
ranges = exon;
if (begin == -1 || exon->begin < begin)
begin = exon->begin;
if (end == -1 || exon->end > end)
end = exon->end;
if( exon_old ){
exon_old->next = exon;
}
exon_old = exon;
}
} catch (ProjectError e) {
cerr << "Constructing GenBank feature: " << e.getMessage() << endl;
throw GBError("GBFeature constructor:Format error when reading genbank format.");
}
}
bool GBFeature::checkRange(int len){
return (begin >= 0 && end >= 0 && begin<len && end<len);
}
/*
* GBFeature::matches
* checks whether other extends this, as mRNA should extend CDS
* in the range covered by this, the ranges should be identical
*/
bool GBFeature::matches(GBFeature &other){
// if genenames exist they should be identical
if (geneid != "" && other.geneid != "" && geneid != other.geneid)
return false;
if (strand != other.strand)
return false;
State *st=ranges, *otherst=other.ranges;
if (!st || !otherst)
return false;
while (otherst && otherst->end < st->end)
otherst = otherst->next;
if (!(otherst && (otherst->begin <= st->begin) && (otherst->end == st->end || (st->next == NULL && otherst->end >= st->end))))
return false;
while (otherst->next && st->next){
st=st->next;
otherst=otherst->next;
if (!(st->next == NULL || st == ranges) // internal exon
&& !(st->begin == otherst->begin && st->end == otherst->end))// doesn't match exactly
return false;
}
if (st->next)
return false;
if (! (otherst->end >= st->end && (otherst->begin == st->begin || (st == ranges && otherst->begin <= st->begin))))
return false;
return true;
}
GBSplitter::GBSplitter( string fname ) : ftype(unknown) {
if (fname != "-") {
ifstrm.open(fname.c_str());
if( !ifstrm )
throw GBError("Could not open input file \"" + fname + "\"!");
} else { // read from standard input
throw GBError("Input from STDIN not supported anymore since the introcution of .gzipped input (with version 3.0).");
//ifstrm.ios::rdbuf(cin.rdbuf());
//ftype = fasta;
}
#ifdef ZIPINPUT
// deflate if gzipped
boost::iostreams::filtering_istream zin;
try {
zin.push(gzip_decompressor());
zin.push(ifstrm);
zin.peek();
if (!zin)
throw("Could not read first character assuming gzip format.");
cout << "# Looks like " << ((fname != "-")? fname: "STDIN")
<< " is in gzip format. Deflating..." << endl;
} catch (...) { // boost::iostreams::gzip_error&
// not a gzip file or ill-formatted
zin.reset();
ifstrm.seekg(0);
zin.push(ifstrm);
}
boost::iostreams::copy(zin, sin);
#else
// only normal file input available
sin << ifstrm.rdbuf();
#endif
determineFileType();
}
GBSplitter::~GBSplitter( ){
if (ifstrm.is_open())
ifstrm.close();
}
void GBSplitter::determineFileType(){
std::stringstream csin(sin.str());
char wrongChar=' ';
ftype = unknown;
csin >> ws;
// check whether it could be genbank format
// search for at least one LOCUS and ORIGIN at the beginning of a line
csin >> goto_line_after( "LOCUS" );
csin >> goto_line_after( "ORIGIN" );
if (csin)
ftype = genbank;
else {
// check, whether it is FASTA or plain sequence
// only letters allowed in the sequence part, except for the end of the line
bool whiteSpaceSeen;
csin.clear();
csin.seekg(0, ios::beg);
string line;
bool haveWrongChar=false;
while (csin && !haveWrongChar) {
getline(csin, line);
if (line[0]!='>') {
whiteSpaceSeen=false;
for (int i=0; i < line.length() && !haveWrongChar; i++)
if (isspace(line[i]))
whiteSpaceSeen = true;
else if (!isalpha(line[i]) && whiteSpaceSeen){
haveWrongChar = true;
wrongChar = line[i];
}
}
}
if (!haveWrongChar)
ftype = fasta;
csin.clear();
csin.seekg(0, ios::beg);
}
if (ftype == unknown) {
string errmsg = "GBProcessor::determineFileType(): Couldn't determine input file type. Found bad character '";
errmsg += wrongChar;
errmsg += "'";
throw GBError(errmsg);
}
// csin.clear();
// csin.seekg(0, ios::beg);
}
GBPositions* GBSplitter::nextData( ){
GBPositions* pos = new GBPositions;
if( !findPositions( *pos ) ){ // No other data available!
delete pos;
return (GBPositions*)0;
}
return pos;
}
AnnoSequence *GBSplitter::getNextFASTASequence( ){
char *sequence = NULL, *name = NULL;
int length;
readOneFastaSeq(sin, sequence, name, length);
if (sequence == NULL || length == 0)
return NULL;
AnnoSequence *seq = new AnnoSequence();
seq->seqname = name;
seq->sequence = sequence;
seq->length = length;
return seq;
}
Boolean GBSplitter::gotoEnd( ){
char buf[GBMAXLINELEN];
do{
int i = 0;
sin.getline( buf, GBMAXLINELEN );
if ((sin.rdstate() & ios_base::failbit) && !(sin.rdstate() & ios_base::eofbit))
sin.clear(sin.rdstate() & ~ios_base::failbit);
while( i < GBMAXLINELEN-1 && isspace(buf[i]))
i++;
if( buf[i] == '/' && buf[i+1] == '/' )
return true;
} while (sin);
return false;
}
Boolean GBSplitter::findPositions( GBPositions& pos ){
int fposb, fpose;
fposb = sin.tellg();
if( !gotoEnd( ) )
return false;
fpose = sin.tellg();
sin.seekg( fposb );
pos.length = fpose-fposb /*+1*/; // Without the '\0'!!
pos.buffer = new char[pos.length+1];
sin.read( pos.buffer, pos.length );
pos.buffer[pos.length] = '\0';
pos.length++; // Now with the '\0'!!!
pos.seqlength = 0;
istringstream isstrm( pos.buffer );
char buf[GBMAXLINELEN];
while( isstrm ){
int curpos = isstrm.tellg();
isstrm >> ws;
isstrm.getline( buf, GBMAXLINELEN-1 );
if ((!sin.eof() && (sin.rdstate() & ios_base::failbit)) || strlen(buf) >= GBMAXLINELEN-2){
throw GBError(string("Could not read the following line in Genbank file.\n") + buf
+ "\nMaximum line length is \n" + itoa(GBMAXLINELEN-2) + ".\n");
}
char *src;
char *rna;
char *cds;
char *seq;
src = strstr( buf, "source" );
if( src && src == buf ){
istringstream strm(buf);
string str;
int a, b;
char c;
strm >> str >> a >> c >> c >> b;
if (b-a+1 > pos.seqlength)
pos.seqlength = b-a+1;
continue;
}
rna = strstr( buf, "mRNA " );
if( rna && rna == buf ){
GBFeature mrnaFeature(pos.buffer+curpos);
if (mrnaFeature.checkRange(pos.seqlength))
pos.mRNA.push_back(mrnaFeature);
else {
cout << "mRNA " << mrnaFeature.geneid << " out of range. Ignoring it." << endl;
cout << "end= " << mrnaFeature.end << " len= " << pos.seqlength << endl;
}
}
cds = strstr( buf, "CDS " );
if( cds && cds == buf ){
GBFeature cdsFeature( pos.buffer+curpos );
if(cdsFeature.checkRange(pos.seqlength))
pos.CDS.push_back( cdsFeature );
else
cout << "CDS " << cdsFeature.geneid << " out of range. Ignoring it." << endl;
}
seq = strstr( buf, "ORIGIN" );
if( seq && seq == buf ){
if (curpos >= pos.length)
cerr << "In gene " << pos.CDS.front().geneid << " a coordinate points after the sequence." << endl;
pos.seqbegin = pos.buffer+curpos;
break;
}
} // while
if (pos.seqlength == 0) {
throw GBError("Sequence has 0 length. Maybe 'source' Feature missing?");
}
return true;
}
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