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package hiseq;
import java.util.ArrayList;
import fileIO.ByteFile;
import shared.Tools;
import structures.IntList;
public class FlowCell {
public FlowCell(String fname){
// TextFile tf=new TextFile(fname);
// for(String line=tf.nextLine(); line!=null; line=tf.nextLine()){
// if(line.charAt(0)=='#'){
//
// }else{
// String[] split=line.split("\t");
// int lane=Integer.parseInt(line)
// }
// }
ByteFile bf=ByteFile.makeByteFile(fname, false);
for(byte[] line=bf.nextLine(); line!=null; line=bf.nextLine()){
if(line[0]=='#'){
String[] split=new String(line).split("\t");
// System.err.println(new String(line));
if(Tools.startsWith(line, "#xSize")){
Tile.xSize=Integer.parseInt(split[1]);
}else if(Tools.startsWith(line, "#ySize")){
Tile.ySize=Integer.parseInt(split[1]);
}
else if(Tools.startsWith(line, "#avgQuality")){
avgQuality=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#avgUnique")){
avgUnique=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#avgErrorFree")){
avgErrorFree=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#avgG")){
avgG=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#stdQuality")){
stdQuality=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#stdUnique")){
stdUnique=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#stdErrorFree")){
stdErrorFree=Double.parseDouble(split[1]);
}else if(Tools.startsWith(line, "#stdG")){
stdG=Double.parseDouble(split[1]);
}
else if(Tools.startsWith(line, "#reads")){
readsProcessed=Long.parseLong(split[1]);
}else if(Tools.startsWith(line, "#avgReads")){
avgReads=Double.parseDouble(split[1]);
}
}else{
int lane=0, tile=0, x1=0, y1=0, x2=0, y2=0, reads=0;
int discard=line[line.length-1]-'0';
int i=0;
for(; i<line.length && line[i]!='\t'; i++){
lane=lane*10+line[i]-'0';
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
tile=tile*10+line[i]-'0';
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
x1=x1*10+line[i]-'0';
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
x2=x2*10+line[i]-'0';
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
y1=y1*10+line[i]-'0';
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
y2=y2*10+line[i]-'0';
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
reads=reads*10+line[i]-'0';
}
i++;
double unique=0, quality=0, error=0;
for(; i<line.length && line[i]!='\t'; i++){
if(line[i]!='.'){
unique=unique*10+line[i]-'0';
}
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
if(line[i]!='.'){
quality=quality*10+line[i]-'0';
}
}
i++;
for(; i<line.length && line[i]!='\t'; i++){
if(line[i]!='.'){
error=error*10+line[i]-'0';
}
}
i++;
unique*=0.001;
quality*=0.001;
error*=0.001;
MicroTile mt=getMicroTile(lane, tile, x1, y1);
assert(mt.x1==x1 && mt.x2==x2 && mt.y1==y1 && mt.y2==y2) : "Micro-tile size seems to be different:\n"
+ "xsize="+Tile.xSize+", ysize="+Tile.ySize;
mt.readCount=reads;
mt.discard=discard;
mt.misses=(int)(unique*reads*0.01);
mt.hits=reads-mt.misses;
mt.qualitySum=reads*quality;
mt.errorFreeSum=reads*error;
// assert(false) : mt.percentErrorFree()+", "+mt.averageQuality()+", "+mt.uniquePercent();
// assert(false) : reads+", "+unique+", "+quality+", "+error+"\n"
// +mt.misses+", "+mt.hits+", "+mt.qualitySum+", "+mt.errorFreeSum+"\n"+new String(line);
}
}
}
public FlowCell(){}
public MicroTile getMicroTile(String id) {//This method is NOT threadsafe
ihp.parse(id);
return getMicroTile(ihp);
}
public MicroTile getMicroTile(IlluminaHeaderParser ihp){
return getLane(ihp.lane).getMicroTile(ihp.tile, ihp.x, ihp.y);
}
public MicroTile getMicroTile(int lane, int tile, int x, int y){
return getLane(lane).getMicroTile(tile, x, y);
}
public Lane getLane(int lane){
while(lanes.size()<=lane){lanes.add(new Lane(lanes.size()));}
return lanes.get(lane);
}
public ArrayList<MicroTile> toList() {
ArrayList<MicroTile> mtList=new ArrayList<MicroTile>();
for(Lane lane : lanes){
if(lane!=null){
for(Tile tile : lane.tiles){
if(tile!=null){
for(ArrayList<MicroTile> ylist : tile.xlist){
if(ylist!=null){
for(MicroTile mt : ylist){
if(mt!=null){
mtList.add(mt);
}
}
}
}
}
}
}
}
return mtList;
}
public ArrayList<MicroTile> calcStats(){
ArrayList<MicroTile> mtList=toList();
readsProcessed=0;
for(MicroTile mt : mtList){
mt.process();
readsProcessed+=mt.readCount;
}
avgReads=readsProcessed*1.0/Tools.max(1, mtList.size());
minCountToUse=(long)Tools.min(500, avgReads*0.25f);
int toKeep=0;
for(MicroTile mt : mtList){
if(mt.readCount>=minCountToUse){toKeep++;}
}
IntList avgQualityList=new IntList(toKeep);
IntList avgUniqueList=new IntList(toKeep);
IntList avgErrorFreeList=new IntList(toKeep);
IntList avgGList=new IntList(toKeep);
for(MicroTile mt : mtList){
if(mt!=null && mt.readCount>=minCountToUse){
avgQualityList.add((int)(1000*mt.averageQuality()));
avgUniqueList.add((int)(1000*mt.uniquePercent()));
avgErrorFreeList.add((int)(1000*mt.percentErrorFree()));
avgGList.add((int)(100000*mt.avgG()));
}
}
int[] avgQualityArray=avgQualityList.toArray();
int[] avgUniqueArray=avgUniqueList.toArray();
int[] avgErrorFreeArray=avgErrorFreeList.toArray();
int[] avgGArray=avgGList.toArray();
avgQuality=Tools.averageDouble(avgQualityArray)*0.001;
avgUnique=Tools.averageDouble(avgUniqueArray)*0.001;
avgErrorFree=Tools.averageDouble(avgErrorFreeArray)*0.001;
avgG=Tools.averageDouble(avgGArray)*0.00001;
stdQuality=Tools.standardDeviation(avgQualityArray)*0.001;
stdUnique=Tools.standardDeviation(avgUniqueArray)*0.001;
stdErrorFree=Tools.standardDeviation(avgErrorFreeArray)*0.001;
stdG=Tools.standardDeviation(avgGArray)*0.00001;
return mtList;
}
public FlowCell widen(int target){
if(readsProcessed<1){
System.err.println("Warning: Zero reads processed.");
return this;
}
if(readsProcessed<target){
return this;
}
FlowCell fc=this;
while(fc.avgReads<target){
FlowCell fc2=fc.widen();
fc2.calcStats();
if(fc2.avgReads<=fc.avgReads){
unwiden();
return fc;
}
fc=fc2;
}
return fc;
}
public void unwiden(){
if(Tile.xSize>Tile.ySize){Tile.ySize/=2;}
else{Tile.xSize/=2;}
}
public FlowCell widen(){
if(Tile.xSize>=Tile.ySize){Tile.ySize*=2;}
else{Tile.xSize*=2;}
System.err.println("Widening to "+Tile.xSize+"x"+Tile.ySize);
ArrayList<MicroTile> list=toList();
FlowCell fc=new FlowCell();
for(MicroTile mt : list){
MicroTile mt2=fc.getMicroTile(mt.lane, mt.tile, mt.x1, mt.y1);
mt2.add(mt);
}
return fc;
}
public ArrayList<Lane> lanes=new ArrayList<Lane>();
long readsProcessed;
public double avgReads;
public double minCountToUse;
public double avgQuality;
public double avgUnique;
public double avgErrorFree;
public double avgG;
public double stdQuality;
public double stdUnique;
public double stdErrorFree;
public double stdG;
private IlluminaHeaderParser ihp=new IlluminaHeaderParser();
}
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