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package icecream;
import java.util.concurrent.ArrayBlockingQueue;
import dna.Data;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.Read;
import stream.SamReadStreamer;
import stream.SamStreamer;
import structures.ListNum;
/**
* Wrapper for a ReadInputStream.
* Produces one ZMW at a time for consumers.
* Allows stopping after X reads or X ZMWs.
* @author Brian Bushnell
* @date June 5, 2020
*/
public class ZMWStreamer implements Runnable {
public ZMWStreamer(FileFormat ff, int queuelen_, long maxReads_, long maxZMWs_){
Data.USE_SAMBAMBA=false;//Sambamba changes PacBio headers.
queuelen=Tools.mid(4, queuelen_, 64);
maxReads=maxReads_;//(maxReads_<0 ? Long.MAX_VALUE : maxReads_);
maxZMWs=maxZMWs_;
// assert(false) : maxReads_+", "+maxReads;
queue=new ArrayBlockingQueue<ZMW>(queuelen);
if(ff.samOrBam() && useStreamer){
cris=null;
ss=makeStreamer(ff);
}else{
cris=makeCris(ff);
ss=null;
}
assert((cris==null) != (ss==null)) : "Exactly one of cris or ss should exist.";
}
public ZMWStreamer(ConcurrentReadInputStream cris_, SamStreamer ss_, int queuelen_){
cris=cris_;
ss=ss_;
queuelen=Tools.mid(4, queuelen_, 64);
maxReads=-1;
maxZMWs=-1;
assert((cris==null) != (ss==null)) : "Exactly one of cris or ss should exist.";
queue=new ArrayBlockingQueue<ZMW>(queuelen);
}
public Thread runStreamer(boolean makeThread){
if(makeThread){
Thread t=new Thread(this);
t.start();
return t;
}else{
run();
return null;
}
}
@Override
public void run(){
if(cris!=null){
handleCris();
}else{
handleStreamer();
}
}
private ConcurrentReadInputStream makeCris(FileFormat ff){
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ff, null, null, null);
cris.start(); //Start the stream
if(verbose){System.err.println("Started cris");}
return cris;
}
private SamReadStreamer makeStreamer(FileFormat ff){
SamReadStreamer ss=new SamReadStreamer(ff, streamerThreads, true, maxReads);
ss.start(); //Start the stream
if(verbose){System.err.println("Started sam streamer");}
return ss;
}
/**
* Pull reads from the cris;
* organize them into lists of subreads from the same ZMW;
* put those lists into the shared queue.
*/
private void handleCris(){
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
ZMW buffer=new ZMW();buffer.id=ZMWs;
long prevZmw=-1;
long readsAdded=0;
// long zmwsAdded=0;
//As long as there is a nonempty read list...
while(ln!=null && ln.size()>0){
for(Read r : ln) {
long zmw;
try {
zmw=Parse.parseZmw(r.id);
} catch (Exception e) {
zmw=r.numericID;//For testing only; disable for production
}
if(zmw<0){zmw=r.numericID;}//For testing only; disable for production
if(verbose){System.err.println("Fetched read "+r.id+"; "+(zmw!=prevZmw)+", "+buffer.isEmpty()+", "+zmw+", "+prevZmw);}
if(zmw!=prevZmw && !buffer.isEmpty()){
ZMWs++;
addToQueue(buffer);
readsAdded+=buffer.size();
// zmwsAdded++;
buffer=new ZMW();buffer.id=ZMWs;
if(maxZMWs>0 && ZMWs>=maxZMWs){break;}
}
buffer.add(r);
prevZmw=zmw;
}
if(maxZMWs>0 && ZMWs>=maxZMWs){break;}
cris.returnList(ln);
//Fetch a new list
ln=cris.nextList();
}
if(!buffer.isEmpty() && (maxZMWs<1 || ZMWs>=maxZMWs)){
ZMWs++;
readsAdded+=buffer.size();
addToQueue(buffer);
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
// cris.returnList(ln.id, true);
}
errorState|=ReadWrite.closeStreams(cris);
addPoison();
}
/**
* Pull reads from the streamer;
* organize them into lists of subreads from the same ZMW;
* put those lists into the shared queue.
*/
private void handleStreamer(){
//Grab the first ListNum of reads
ListNum<Read> ln=ss.nextList();
ZMW buffer=new ZMW();buffer.id=ZMWs;
long prevZmw=-1;
long added=0;
//As long as there is a nonempty read list...
while(ln!=null && ln.size()>0){
for(Read r : ln) {
long zmw;
try {
zmw=Parse.parseZmw(r.id);
} catch (Exception e) {
zmw=r.numericID;//For testing only; disable for production
}
if(zmw<0){zmw=r.numericID;}//For testing only; disable for production
if(verbose){System.err.println("Fetched read "+r.id+"; "+(zmw!=prevZmw)+", "+buffer.isEmpty()+", "+zmw+", "+prevZmw);}
if(zmw!=prevZmw && !buffer.isEmpty()){
ZMWs++;
addToQueue(buffer);
added+=buffer.size();
buffer=new ZMW();buffer.id=ZMWs;
}
buffer.add(r);
prevZmw=zmw;
}
//Fetch a new list
ln=ss.nextList();
}
if(!buffer.isEmpty()){
ZMWs++;
added+=buffer.size();
addToQueue(buffer);
}
addPoison();
}
private void addPoison(){
// //Notify worker threads that there is no more data
// for(int i=0; i<threads; i++){
// addToQueue(POISON);
// }
addToQueue(POISON);
}
private void addToQueue(ZMW buffer){
if(verbose) {System.err.println("Adding to queue "+(buffer==POISON ? "poison" : buffer.get(0).id));}
while(buffer!=null) {
try {
queue.put(buffer);
buffer=null;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
}
public ZMW nextZMW(){
ZMW buffer=null;
while(buffer==null) {
try {
buffer=queue.take();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
if(verbose){System.err.println("Pulled from queue "+(buffer==POISON ? "poison" : buffer.get(0).id));}
if(buffer==POISON){
addToQueue(POISON);
return null;
}else{
return buffer;
}
}
private final ConcurrentReadInputStream cris;
private final SamStreamer ss;
private final int queuelen;
public long ZMWs=0;
private final long maxReads;
private final long maxZMWs;
public boolean errorState=false;
private final ArrayBlockingQueue<ZMW> queue;
private static final ZMW POISON=new ZMW(0);
public static boolean verbose=false;
//Streamer seems to give more highly variable timings... sometimes. And it's not really needed.
public static boolean useStreamer=false;
//Only 1 thread for now to force ordered input
public static final int streamerThreads=1;
}
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