1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243
|
package stream;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.ArrayBlockingQueue;
import fileIO.ByteFile;
import fileIO.FileFormat;
import shared.Shared;
import shared.Timer;
import structures.ListNum;
/**
* Loads sam files rapidly with multiple threads.
*
* @author Brian Bushnell
* @date November 4, 2016
*
*/
public abstract class SamStreamer {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static final void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
int threads=Shared.threads();
if(args.length>1){threads=Integer.parseInt(args[1]);}
SamStreamer x=new SamReadStreamer(args[0], threads, false, -1);
//Run the object
x.start();
x.test();
t.stop("Time: ");
}
/**
* Constructor.
*/
public SamStreamer(String fname_, int threads_, boolean saveHeader_, long maxReads_){
this(FileFormat.testInput(fname_, FileFormat.SAM, null, true, false), threads_, saveHeader_, maxReads_);
}
/**
* Constructor.
*/
public SamStreamer(FileFormat ffin_, int threads_, boolean saveHeader_, long maxReads_){
fname=ffin_.name();
threads=threads_;
ffin=ffin_;
saveHeader=saveHeader_;
header=(saveHeader ? new ArrayList<byte[]>() : null);
maxReads=(maxReads_<1 ? Long.MAX_VALUE : maxReads_);
inq=new ArrayBlockingQueue<ListNum<byte[]>>(threads/2+1);
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
final void test(){
for(ListNum<Read> list=nextReads(); list!=null; list=nextReads()){
if(verbose){outstream.println("Got list of size "+list.size());}
}
}
/** Create read streams and process all data */
public final void start(){
//Reset counters
readsProcessed=0;
basesProcessed=0;
//Process the reads in separate threads
spawnThreads();
if(verbose){outstream.println("Finished; closing streams.");}
}
public final ListNum<Read> nextList(){return nextReads();}
public abstract ListNum<Read> nextReads();
public abstract ListNum<SamLine> nextLines();
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
public final void processBytes0(int tid){
if(verbose){outstream.println("tid "+tid+" started processBytes.");}
// ByteFile.FORCE_MODE_BF1=true;
ByteFile.FORCE_MODE_BF2=true;
ByteFile bf=ByteFile.makeByteFile(ffin);
long listNumber=0;
long reads=0;
final int limit=LIST_SIZE;
ArrayList<byte[]> list=new ArrayList<byte[]>(limit);
for(byte[] line=bf.nextLine(); line!=null && reads<maxReads; line=bf.nextLine()){
assert(line!=null);
// outstream.println("a");
if(header!=null && line[0]=='@'){
if(Shared.TRIM_RNAME){line=SamReadInputStream.trimHeaderSQ(line);}
header.add(line);
}else{
if(header!=null){
SamReadInputStream.setSharedHeader(header);
header=null;
}
reads++;
list.add(line);
if(list.size()>=limit){
// outstream.println("b");
// outstream.println(inq.size()+", "+inq.remainingCapacity());
putBytes(new ListNum<byte[]>(list, listNumber));
listNumber++;
// outstream.println("c");
list=new ArrayList<byte[]>(limit);
}
}
// outstream.println("d");
}
if(header!=null){
SamReadInputStream.setSharedHeader(header);
header=null;
}
if(verbose){outstream.println("tid "+tid+" ran out of input.");}
if(list.size()>0){
putBytes(new ListNum<byte[]>(list, listNumber));
listNumber++;
list=null;
}
if(verbose || verbose2){outstream.println("tid "+tid+" done reading bytes.");}
putBytes(POISON_BYTES);
if(verbose || verbose2){outstream.println("tid "+tid+" done poisoning.");}
bf.close();
if(verbose || verbose2){outstream.println("tid "+tid+" closed stream.");}
}
final void putBytes(ListNum<byte[]> list){
// if(verbose){outstream.println("tid "+tid+" putting blist size "+list.size());}
while(list!=null){
try {
inq.put(list);
list=null;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
// if(verbose){outstream.println("tid "+tid+" done putting blist");}
}
final ListNum<byte[]> takeBytes(){
// if(verbose){outstream.println("tid "+tid+" taking blist");}
ListNum<byte[]> list=null;
while(list==null){
try {
list=inq.take();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
// if(verbose){outstream.println("tid "+tid+" took blist size "+list.size());}
return list;
}
/** Spawn process threads */
abstract void spawnThreads();
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file path */
protected String fname;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
// public void setMaxReads(long x){
// maxReads=(x<1 ? Long.MAX_VALUE : x);
// }
/** Quit after processing this many input reads */
private final long maxReads;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
final boolean saveHeader;
/** Primary input file */
final FileFormat ffin;
final ArrayBlockingQueue<ListNum<byte[]>> inq;
final int threads;
ArrayList<byte[]> header;
/*--------------------------------------------------------------*/
/*---------------- Static Fields ----------------*/
/*--------------------------------------------------------------*/
static final ListNum<Read> POISON_READS=new ListNum<Read>(null, -1);
static final ListNum<SamLine> POISON_LINES=new ListNum<SamLine>(null, -1);
static final ListNum<byte[]> POISON_BYTES=new ListNum<byte[]>(null, -1);
public static int LIST_SIZE=200;
public static int DEFAULT_THREADS=6;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
protected PrintStream outstream=System.err;
/** Print verbose messages */
public static final boolean verbose=false;
public static final boolean verbose2=false;
/** True if an error was encountered */
public boolean errorState=false;
}
|