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package tax;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.Collections;
import java.util.List;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextFile;
import fileIO.TextStreamWriter;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.ReadStats;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.ListNum;
/**
* Filters sequences according to their taxonomy,
* as determined by the sequence name. Sequences should
* be labeled with a gi number or NCBI taxID.
*
* @author Brian Bushnell
* @date November 23, 2015
*
*/
public class PrintTaxonomy {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer();
PrintTaxonomy x=new PrintTaxonomy(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public PrintTaxonomy(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.MAX_ZIP_THREADS=Shared.threads();
//Create a parser object
Parser parser=new Parser();
int taxLevel=0, minLevel=0, maxLevel=TaxTree.LIFE;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(a.equals("out")){
out1=b;
}else if(a.equals("counts")){
countFile=b;
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("table") || a.equals("gi") || a.equals("gitable")){
giTableFile=b;
}else if(a.equals("accession")){
accessionFile=b;
}else if(a.equals("tree") || a.equals("taxtree")){
taxTreeFile=b;
}else if(a.equals("level") || a.equals("lv") || a.equals("taxlevel") || a.equals("tl")){
taxLevel=TaxTree.parseLevel(b);
}else if(a.equals("minlevel")){
minLevel=TaxTree.parseLevel(b);
}else if(a.equals("maxlevel")){
maxLevel=TaxTree.parseLevel(b);
}else if(a.equals("printname")){
printName=Parse.parseBoolean(b);
}else if(a.equals("reverse")){
reverseOrder=Parse.parseBoolean(b);
}else if(a.equals("silva")){
TaxTree.SILVA_MODE=Parse.parseBoolean(b);
}else if(a.equals("unite")){
TaxTree.UNITE_MODE=Parse.parseBoolean(b);
}else if(a.equals("simple")){
skipNonCanonical=Parse.parseBoolean(b);
}else if(a.equals("column")){
keyColumn=Integer.parseInt(b);
}else if(b!=null && (a.equals("name") || a.equals("names") || a.equals("id") || a.equals("ids"))){
for(String s : b.split(",")){
names.add(s);
}
}else{
names.add(arg);
}
}
if(taxTreeFile==null || "auto".equalsIgnoreCase(taxTreeFile)){taxTreeFile=TaxTree.defaultTreeFile();}
if("auto".equalsIgnoreCase(giTableFile)){giTableFile=TaxTree.defaultTableFile();}
if("auto".equalsIgnoreCase(accessionFile)){accessionFile=TaxTree.defaultAccessionFile();}
taxLevelExtended=TaxTree.levelToExtended(taxLevel);
minLevelExtended=TaxTree.levelToExtended(minLevel);
maxLevelExtended=TaxTree.levelToExtended(maxLevel);
{//Process parser fields
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
in1=parser.in1;
maxReads=parser.maxReads;
}
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output file "+out1+"\n");
}
//Create output FileFormat objects
ffout1=FileFormat.testOutput(out1, FileFormat.TEXT, null, true, overwrite, append, false);
ffcount=FileFormat.testOutput(countFile, FileFormat.TEXT, null, true, overwrite, append, false);
//Create input FileFormat objects
ffin1=FileFormat.testInput(in1, FileFormat.TEXT, null, true, false);
if(giTableFile!=null){
outstream.println("Loading gi table.");
GiToTaxid.initialize(giTableFile);
}
if(accessionFile!=null){
outstream.println("Loading accession table.");
AccessionToTaxid.load(accessionFile);
}
if(taxTreeFile!=null){
tree=TaxTree.loadTaxTree(taxTreeFile, outstream, true, true);
assert(tree.nameMap!=null);
}else{
tree=null;
throw new RuntimeException("No tree specified.");
}
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
TextStreamWriter tsw=null;
if(ffout1!=null){
tsw=new TextStreamWriter(ffout1);
tsw.start();
}
if(ffin1!=null){
if(ffin1.fasta() || ffin1.fastq() || ffin1.samOrBam() || ffin1.scarf()){
processReads(tsw);
}else{
processFile(new TextFile(ffin1), tsw);
}
}else{
processNames(tsw);
}
if(tsw!=null){errorState|=tsw.poisonAndWait();}
if(ffcount!=null){
TextStreamWriter tswc=new TextStreamWriter(ffcount);
tswc.start();
for(TaxNode tn : tree.nodes){
if(tn!=null && tn.countRaw>0){
tswc.println(tn.countRaw+"\t"+tn.name);
}
}
errorState|=tswc.poisonAndWait();
}
t.stop();
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/** Iterate through the names */
void processNames(final TextStreamWriter tsw){
for(String name : names){
if(taxLevelExtended>0){
printTaxLevel(name, tsw);
}else{
printTaxonomy(name, tsw);
}
}
}
/** Iterate through the names */
void processFile(final TextFile tf, final TextStreamWriter tsw){
for(String name=tf.nextLine(); name!=null; name=tf.nextLine()){
if(keyColumn>=0){
String result=translateLine(name, keyColumn);
tsw.print(result);
}else if(taxLevelExtended>0){
printTaxLevel(name, tsw);
}else{
printTaxonomy(name, tsw);
}
}
}
/** Iterate through the names */
void processReads(final TextStreamWriter tsw){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, null);
if(verbose){System.err.println("Started cris");}
cris.start();
}
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
for(Read r1 : reads){
if(keyColumn>=0){
String result=translateLine(r1.id, keyColumn);
tsw.println(result);
}else if(taxLevelExtended>0){
printTaxLevel(r1.id, tsw);
}else{
printTaxonomy(r1.id, tsw);
}
}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
cris.returnList(ln);
ReadWrite.closeStreams(cris);
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
String translateLine(String line, int col){
StringBuilder sb=new StringBuilder();
String[] split=line.split("\t");
assert(split.length>col) : "Too few columns in line:\n"+line+"\n->\n"+Arrays.toString(split);
if(col<split.length){
String name=split[col];
while(name.startsWith(">") || name.startsWith("@")){name=name.substring(1);}
TaxNode tn=parseNodeFromHeader(name);
if(tn!=null){
String tl=makeTaxLine(tn, minLevelExtended, maxLevelExtended).toString();
split[col]=tl;
}else{
List<TaxNode> list=tree.getNodesByNameExtended(name);
if(list!=null){
String tab="";
for(TaxNode tn2 : list){
sb.append(tab);
sb.append(makeTaxLine(tn2, minLevelExtended, maxLevelExtended).toString());
tab="\t";
}
}else{
split[col]=split[col]+"_***NOT_FOUND***";
}
}
}
for(int i=0; i<split.length; i++){
if(i>0){sb.append('\t');}
sb.append(split[i]);
}
sb.append('\n');
return sb.toString();
}
void printTaxonomy(String name, final TextStreamWriter tsw){
while(name.startsWith(">") || name.startsWith("@")){name=name.substring(1);}
tsw.print("\n");
if(printName){tsw.print(name+":\n");}
TaxNode tn=parseNodeFromHeader(name);
if(tn!=null){
printTaxonomy(tn, tsw);
return;
}else{
List<TaxNode> list=tree.getNodesByNameExtended(name);
if(list!=null){
String nl="";
for(TaxNode tn2 : list){
tsw.print(nl);
printTaxonomy(tn2, tsw);
nl="\n";
}
return;
}
}
tsw.println("Could not find node" + (printName ? "." : " for '"+name+"'"));
return;
}
void printTaxLevel(String name, final TextStreamWriter tsw){
while(name.startsWith(">") || name.startsWith("@")){name=name.substring(1);}
tsw.print("\n");
if(printName){tsw.print(name+":\n");}
TaxNode tn=parseNodeFromHeader(name);
if(tn!=null){
printTaxLevel(tn, tsw);
return;
}else{
List<TaxNode> list=tree.getNodesByNameExtended(name);
if(list!=null){
for(TaxNode tn2 : list){
printTaxLevel(tn2, tsw);
}
return;
}
}
tsw.println("Could not find node" + (printName ? "." : " for '"+name+"'"));
return;
}
// void printTaxCounts(String name, final TextStreamWriter tsw){
// TaxNode tn=null;
// tn=tree.getNode(name);
// if(tn==null){tn=tree.getNodeByName(name);}
// if(tn==null){tn=unknown;}
// while(tn!=null && tn.id!=tn.pid && tn.level<taxLevel){tn=tree.getNode(tn.pid);}
// if(tsw!=null)tsw.println(tn.name);
// tn.incrementRaw(1);
// }
void printTaxonomy(TaxNode tn, final TextStreamWriter tsw){
// assert(false) : tn.levelExtended+", "+taxLevelExtended+", "+minLevelExtended+", "+maxLevelExtended;
assert(tn!=null);
// tsw.print("\n");
do{
if(tn.levelExtended<=taxLevelExtended){tn.incrementRaw(1);}
if(tn.levelExtended>=minLevelExtended && tn.levelExtended<=maxLevelExtended){
if(!tn.cellularOrganisms() && (!skipNonCanonical || tn.isSimple())){
tsw.println(tn.levelStringExtended(false)+"\t"+tn.id+"\t"+tn.name);
}
}
tn=tree.getNode(tn.pid);
}while(tn!=null && tn.id!=tn.pid);
}
StringBuilder makeTaxLine(TaxNode tn, int minLevelE, int maxLevelE){
// assert(false) : tn+", "+minLevelE+", "+maxLevelE;
assert(tn!=null);
StringBuilder sb=new StringBuilder();
if(reverseOrder){
ArrayList<TaxNode> list=new ArrayList<TaxNode>();
while(tn.levelExtended<=maxLevelE){
if(tn.levelExtended>=minLevelE){
if(!tn.cellularOrganisms() && (!skipNonCanonical || tn.isSimple())){
list.add(tn);
}
}
if(tn.id==tn.pid){break;}
tn=tree.getNode(tn.pid);
}
String semi="";
Collections.reverse(list);
for(TaxNode tn2 : list){
sb.append(semi);
sb.append(tn2.levelToStringShort());
sb.append("__");
sb.append(tn2.name);
semi=";";
}
}else{
String semi="";
while(tn.levelExtended<=maxLevelE){
if(tn.levelExtended>=minLevelE && !tn.cellularOrganisms() && (!skipNonCanonical || tn.isSimple())){
sb.append(semi);
sb.append(tn.levelToStringShort());
sb.append("__");
sb.append(tn.name);
semi=";";
}
if(tn.id==tn.pid){break;}
tn=tree.getNode(tn.pid);
}
}
return sb;
}
// public static void printTaxonomy(TaxNode tn, final StringBuilder sb, final TaxTree tree, final int maxLevel, boolean skipNonCanonical){
// final int maxLevelE=maxLevel<0 ? maxLevel : TaxTree.levelToExtended(maxLevel);
// assert(tn!=null);
//// tsw.print("\n");
// do{
// if(!tn.cellularOrganisms() && (!skipNonCanonical || tn.isSimple())){
// sb.append(tn.levelStringExtended(false)+"\t"+tn.id+"\t"+tn.name+"\n");
// }
// tn=tree.getNode(tn.pid);
// }while(tn!=null && tn.id!=tn.pid && tn.levelExtended<=maxLevelE);
// }
public static void printTaxonomy(TaxNode tn, final ByteBuilder sb, final TaxTree tree, final int maxLevel, boolean skipNonCanonical){
final int maxLevelE=maxLevel<0 ? maxLevel : TaxTree.levelToExtended(maxLevel);
assert(tn!=null);
// tsw.print("\n");
do{
if(!tn.cellularOrganisms() && (!skipNonCanonical || tn.isSimple())){
sb.append(tn.levelStringExtended(false)).append('\t').append(tn.id).append('\t').append(tn.name).append('\n');
}
tn=tree.getNode(tn.pid);
}while(tn!=null && tn.id!=tn.pid && tn.levelExtended<=maxLevelE);
}
// public static void printTaxonomy(TaxNode tn, final TextStreamWriter tsw, final TaxTree tree, final int maxLevel){
// final int maxLevelE=maxLevel<0 ? maxLevel : TaxTree.levelToExtended(maxLevel);
// assert(tn!=null);
//// tsw.print("\n");
// do{
// if(!skipNonCanonical || tn.isSimple()){
// tsw.println(tn.levelStringExtended(false)+"\t"+tn.id+"\t"+tn.name);
// }
// tn=tree.getNode(tn.pid);
// }while(tn!=null && tn.id!=tn.pid && tn.levelExtended<=maxLevelE);
// }
void printTaxLevel(TaxNode tn, final TextStreamWriter tsw){
if(tn==null){tn=unknown;}
while(tn.id!=tn.pid && tn.levelExtended<taxLevelExtended){tn=tree.getNode(tn.pid);}
if(tsw!=null){tsw.println(tn.name);}
tn.incrementRaw(1);
}
// void printTaxCounts(TaxNode tn, final TextStreamWriter tsw){
// if(tn==null){tn=unknown;}
// while(tn!=null && tn.id!=tn.pid && tn.level<taxLevel){tn=tree.getNode(tn.pid);}
// if(tsw!=null)tsw.println(tn.name);
// tn.incrementRaw(1);
// }
public TaxNode parseNodeFromHeader(String header){
if(tree==null){return null;}
return tree.parseNodeFromHeader(header, true);
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Optional input file path */
private String in1=null;
/** Primary output file path */
private String out1="stdout.txt";
private String countFile=null;
private String giTableFile=null;
private String taxTreeFile=null;
private String accessionFile=null;
private final TaxTree tree;
// /** Level to print */
// private int taxLevel=-1;//TaxTree.stringToLevel("phylum");
//
// /** Min level to print */
// private int minLevel=-1;
//
// /** Max level to print */
// private int maxLevel=TaxTree.stringToLevel("life");
private final int taxLevelExtended, minLevelExtended, maxLevelExtended;
/** Reverse order for tax lines */
private boolean reverseOrder=true;
private ArrayList<String> names=new ArrayList<String>();
private long maxReads=-1;
boolean printName=true;
boolean skipNonCanonical=false;
int keyColumn=-1;
// Deprecated. Description from shellscript:
// column=-1 If set to a non-negative integer, parse the taxonomy
// information from this column in a tab-delimited file.
// Example if column=1:
// read1 TAB gi|944259871|gb|KQL24128.1| TAB score:42
// becomes
// read1 TAB k__Viridiplantae;p__Streptophyta;... TAB score:42
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Optional input file */
private final FileFormat ffin1;
/** Primary output file */
private final FileFormat ffout1;
private final FileFormat ffcount;
private final TaxNode unknown=new TaxNode(-99, -99, TaxTree.LIFE, TaxTree.LIFE_E, "UNKNOWN");
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=false;
/** Append to existing output files */
private boolean append=false;
}
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