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package bin;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.locks.ReadWriteLock;
import shared.KillSwitch;
import shared.LineParserS1;
import shared.LineParserS4;
import shared.Shared;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.Read;
import structures.IntLongHashMap;
import structures.ListNum;
import template.Accumulator;
import template.ThreadWaiter;
import tracker.EntropyTracker;
public class SpectraCounter implements Accumulator<SpectraCounter.LoadThread> {
public SpectraCounter(PrintStream outstream_, boolean parseDepth_,
boolean parseTID_, IntLongHashMap sizeMap_) {
outstream=outstream_;
parseDepth=parseDepth_;
parseTID=parseTID_;
sizeMap=sizeMap_;
if(calcEntropy) {
if(AdjustEntropy.kLoaded!=4 || AdjustEntropy.wLoaded!=150) {
AdjustEntropy.load(4, 150);
}
assert(AdjustEntropy.kLoaded==4 && AdjustEntropy.wLoaded==150) :
AdjustEntropy.kLoaded+", "+calcEntropy;
}
}
/** Spawn process threads */
public void makeSpectra(ArrayList<Contig> contigs, ConcurrentReadInputStream cris, int minlen){
//Do anything necessary prior to processing
// sizeMap=(parseTax ? new IntLongHashMap(1021) : null);
//Determine how many threads may be used
int threads=Tools.mid(1, cris==null ? contigs.size()/4 : 128, Shared.threads());
//Fill a list with LoadThreads
ArrayList<LoadThread> alpt=new ArrayList<LoadThread>(threads);
for(int i=0; i<threads; i++){
LoadThread lt=new LoadThread(contigs, cris, minlen, i, threads);
alpt.add(lt);
}
//Start the threads and wait for them to finish
boolean success=ThreadWaiter.startAndWait(alpt, this);
errorState&=!success;
//Do anything necessary after processing
}
@Override
public synchronized void accumulate(LoadThread t) {
synchronized(t) {
errorState|=(t.success);
contigsLoaded+=t.contigsLoadedT;
basesLoaded+=t.basesLoadedT;
contigsRetained+=t.contigsRetainedT;
basesRetained+=t.basesRetainedT;
}
}
@Override
public ReadWriteLock rwlock() {return null;}
@Override
public synchronized boolean success() {return errorState;}
class LoadThread extends Thread {
LoadThread(ArrayList<Contig> contigs_, ConcurrentReadInputStream cris_,
int minlen_, int tid_, int threads_) {
contigs=contigs_;
cris=cris_;
minlen=minlen_;
tid=tid_;
threads=threads_;
}
@Override
public void run() {
synchronized(this) {
runInner();
}
}
private void runInner() {
if(cris==null) {//Calculate data on existing contigs
runOnContigs();
}else {//Load contigs concurrently
runOnCris();
}
success=true;
}
void runOnContigs() {
for(int i=tid; i<contigs.size(); i+=threads) {
Contig c=contigs.get(i);
processContig(c);
}
}
void runOnCris() {
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//As long as there is a nonempty read list...
while(ln!=null && ln.size()>0){
ArrayList<Contig> localContigs=new ArrayList<Contig>(ln.size());
for(Read r : ln) {
Contig c=loadContig(r);
if(c!=null) {
processContig(c);
localContigs.add(c);
}
}
synchronized(contigs) {contigs.addAll(localContigs);}
//Notify the input stream that the list was used
cris.returnList(ln);
// if(verbose){outstream.println("Returned a list.");} //Disabled due to non-static access
//Fetch a new list
ln=cris.nextList();
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
Contig processRead(Read r) {
Contig c=loadContig(r);
if(c==null) {return null;}
processContig(c);
return c;
}
Contig loadContig(Read r) {
contigsLoadedT++;
basesLoadedT+=r.length();
int tid=-1;
if(parseTID) {
tid=DataLoader.parseTaxID(r.name());
if(tid>0) {
synchronized(sizeMap) {
sizeMap.increment(tid, r.length());
}
}
}
if(r.length()<minlen) {return null;}
contigsRetainedT++;
basesRetainedT+=r.length();
Contig c=new Contig(r.name(), r.bases, (int)r.numericID);
synchronized(c) {
c.labelTaxid=tid;
}
return c;
}
void processContig(Contig c) {
synchronized(c) {
// System.err.println("Thread "+tid+" got lock on "+c.name+", "+c.id()+", "+c.size());
contigsProcessedT++;
basesProcessedT+=c.size();
c.loadCounts();
if(c.numDepths()>1) {c.fillNormDepth();}
if(calcEntropy) {
c.entropy=et.averageEntropy(c.bases, false);
c.entropy=AdjustEntropy.compensate(c.gc(), c.entropy);
}
if(calcStrandedness) {
c.dimers=new int[16];
c.strandedness=EntropyTracker.strandedness(c.bases, c.dimers, 2);
}
if(countTrimers) {
c.trimers=new int[64];
EntropyTracker.countKmers(c.bases, c.trimers, 3);
}
if(parseDepth) {
boolean b=DataLoader.parseAndSetDepth(c, lps, lpt);
if(!b) {
KillSwitch.kill("Could not parse depth from header "+c.name+
"\nThis program needs a sam file, a cov file, or labeled contigs.");
}
assert(b) : "Could not parse depth from "+c.name;
}
assert(c.tetramers!=null && c.numTetramers>0);
}
}
final int tid;
final int threads;
final int minlen;
final ArrayList<Contig> contigs;
final ConcurrentReadInputStream cris;
final EntropyTracker et=new EntropyTracker(entropyK, entropyWindow, false);
// final int[] counts=(calcEntropy ? new int[1<<(entropyK*2)] : null);
boolean success=false;
int contigsProcessedT=0;
long basesProcessedT=0;
LineParserS1 lps=new LineParserS1('_');
LineParserS4 lpt=new LineParserS4(",,=,");
int contigsLoadedT=0;
long basesLoadedT=0;
int contigsRetainedT=0;
long basesRetainedT=0;
}
public PrintStream outstream=System.err;
public final boolean parseDepth;
public final boolean parseTID;
public final IntLongHashMap sizeMap;
public int contigsLoaded=0;
public long basesLoaded=0;
public int contigsRetained=0;
public long basesRetained=0;
public boolean errorState=false;
public static boolean calcEntropy=true;
public static boolean calcStrandedness=true;
public static boolean countTrimers=true;
public static int entropyK=4;
public static int entropyWindow=150;
}
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