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package sketch;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.HashMap;
import java.util.Map.Entry;
import java.util.concurrent.atomic.AtomicInteger;
import fileIO.ByteFile;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import structures.ByteBuilder;
import structures.FloatList;
import tax.TaxTree;
import template.ThreadWaiter;
/**
* @author Brian Bushnell
* @date May 9, 2016
*
*/
public class AnalyzeSketchResults {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
AnalyzeSketchResults x=new AnalyzeSketchResults(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public AnalyzeSketchResults(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, /*getClass()*/null, false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
{//Parse the arguments
final Parser parser=parse(args);
overwrite=parser.overwrite;
append=parser.append;
in1=parser.in1;
in2=parser.in2;
out1=parser.out1;
}
fixExtensions(); //Add or remove .gz or .bz2 as needed
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
ffout1=FileFormat.testOutput(out1, FileFormat.TXT, null, true, overwrite, append, false);
ffoutMap=FileFormat.testOutput(outMap, FileFormat.TXT, null, true, overwrite, append, false);
ffoutAccuracy=FileFormat.testOutput(outAccuracy, FileFormat.TXT, null, true, overwrite, append, false);
ffoutBad=FileFormat.testOutput(outBad, FileFormat.TXT, null, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.TXT, null, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.TXT, null, true, true);
recordSets=(ffoutAccuracy==null && ffoutBad==null ? null : new ArrayList<RecordSet>());
tree=(treeFile==null) ? null : TaxTree.loadTaxTree(treeFile, outstream, true, false);
SSUMap.load(outstream);
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("map") || a.equals("outmap")){
outMap=b;
}else if(a.equals("accuracy") || a.equals("outacc") || a.equals("outaccuracy")){
outAccuracy=b;
}else if(a.equals("outbad")){
outBad=b;
}else if(a.equals("tree")){
treeFile=b;
}else if(a.equals("shrinkonly")){
shrinkOnly=Parse.parseBoolean(b);
}else if(a.equals("ssu") || a.equals("ssufile")){
assert(false) : "ssu and ssufile are deprecated; please specify 16S or 18S independently";
}else if(a.equalsIgnoreCase("16S") || a.equalsIgnoreCase("16Sfile")){
SSUMap.r16SFile=b;
}else if(a.equalsIgnoreCase("18S") || a.equalsIgnoreCase("18Sfile")){
SSUMap.r18SFile=b;
}else if(a.equals("mash")){
mode=MASH_MODE;
}else if(a.equals("sourmash")){
mode=SOURMASH_MODE;
}else if(a.equals("blast")){
mode=BLAST_MODE;
}else if(a.equals("bbsketch")){
mode=BBSKETCH_MODE;
}else if(a.equals("lines")){
maxLines=Long.parseLong(b);
if(maxLines<0){maxLines=Long.MAX_VALUE;}
}else if(a.equals("minsamples")){
minSamples=Integer.parseInt(b);
}else if(a.equals("verbose")){
RecordSet.verbose=Record.verbose=verbose=Parse.parseBoolean(b);
}else if(parser.parse(arg, a, b)){
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
return parser;
}
/** Add or remove .gz or .bz2 as needed */
private void fixExtensions(){
in1=Tools.fixExtension(in1);
in2=Tools.fixExtension(in2);
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1, outMap)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output file "+out1+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1, in2)){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, in2, out1, outMap)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
// if(!ByteFile.FORCE_MODE_BF2){
// ByteFile.FORCE_MODE_BF2=false;
// ByteFile.FORCE_MODE_BF1=true;
// }
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
void process(Timer t){
if(verbose){System.err.println("process()");}
ByteFile bf1=ByteFile.makeByteFile(ffin1);
ByteFile bf2=(ffin2==null ? null : ByteFile.makeByteFile(ffin2));
if(shrinkOnly){
runShrinkOnly(bf1);
return;
}
bswBad=makeBSW(ffoutBad);
ResultLineParser parser=new ResultLineParser(mode, tree, bswBad, recordSets, false);
processInner(bf1, parser, ffin2==null ? null : aniMap);
ByteStreamWriter bsw=makeBSW(ffout1);
printResults(parser, bsw);
if(bsw!=null){errorState|=bsw.poisonAndWait();}
ByteStreamWriter bswAcc=makeBSW(ffoutAccuracy);
if(recordSets!=null){
if(SSUMap.hasMap()){
processSetsThreaded();
}
printAccuracy(bswAcc);
}
if(bswAcc!=null){errorState|=bswAcc.poisonAndWait();}
if(bswBad!=null){errorState|=bswBad.poisonAndWait();}
ByteStreamWriter bswMap=makeBSW(ffoutMap);
if(bf2!=null){
processInner(bf2, parser, aaiMap);
printMap(bswMap);
}
if(bswMap!=null){errorState|=bswMap.poisonAndWait();}
t.stop();
outstream.println(Tools.timeLinesBytesProcessed(t, linesProcessed, bytesProcessed, 8));
outstream.println();
outstream.println("Lines Out: \t"+linesOut);
outstream.println("Bytes Out: \t"+bytesOut);
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
private void runShrinkOnly(ByteFile bf){
if(verbose){System.err.println("runShrinkOnly("+bf.name()+")");}
ResultLineParser parser=new ResultLineParser(mode, tree, null, null, true);
ByteStreamWriter bsw=makeBSW(ffout1);
byte[] line=bf.nextLine();
while(line!=null){
if(line.length>0){
if(maxLines>0 && linesProcessed>=maxLines){break;}
linesProcessed++;
bytesProcessed+=(line.length+1);
parser.parse(line);
RecordSet rs=parser.processData(null, true);
if(rs!=null){
rs.sortAndSweep();
for(Record r : rs.records){
if(bsw!=null){
bsw.println(r.text);
}
}
}
}
line=bf.nextLine();
}
errorState|=bf.close();
RecordSet rs=parser.currentSet;
if(rs!=null){
rs.sortAndSweep();
for(Record r : rs.records){
if(bsw!=null){
bsw.println(r.text);
}
}
}
if(bsw!=null){errorState|=bsw.poisonAndWait();}
}
private void processInner(ByteFile bf, ResultLineParser parser, HashMap<Long, Float> map){
if(verbose){System.err.println("processInner("+bf.name()+")");}
byte[] line=bf.nextLine();
while(line!=null){
if(line.length>0){
if(maxLines>0 && linesProcessed>=maxLines){break;}
linesProcessed++;
bytesProcessed+=(line.length+1);
parser.parse(line);
parser.processData(map, recordSets!=null);
}
line=bf.nextLine();
}
errorState|=bf.close();
}
private void processSetsThreaded(){
if(verbose){System.err.println("processSetsThreaded("+Shared.threads()+")");}
final int threads=Shared.threads();
// final ThreadWaiter waiter=new ThreadWaiter();
final ArrayList<SSUThread> list=new ArrayList<SSUThread>(threads);
final AtomicInteger atom=new AtomicInteger(0);
for(int i=0; i<threads; i++){
list.add(new SSUThread(atom));
}
boolean success=ThreadWaiter.startAndWait(list);
if(!success){errorState=true;}
}
private void printAccuracy(ByteStreamWriter bswAcc){
if(verbose){System.err.println("printAccuracy("+bswAcc+")");}
int[][] results=new int[taxLevels][16];
for(RecordSet rs : recordSets){
if(mode==MASH_MODE){
rs.sortAndSweep();
rs.processSSU();
}
int[] statusArray=rs.test(bswBad);
for(int level=0; level<statusArray.length; level++){
int status=statusArray[level];
results[level][status]++;
assert(status==NOHIT || status==CORRECT ||
status==INCORRECT_TAX_CORRECT_SSU || status==INCORRECT_TAX_INCORRECT_SSU ||
status==INCORRECT_TAX_MISSING_SSU) : status;
}
}
ByteBuilder bb=new ByteBuilder();
bb.append("#Level \tCorrect\tbadTaxGoodSSU\tBadTaxNoSSU\tbadTaxBadSSU\tNoHit\n");
if(bswAcc!=null){bswAcc.print(bb);}
for(String levelName : printLevels){
int level=TaxTree.stringToLevelExtended(levelName);
bb.clear();
bb.append(TaxTree.levelToStringExtended(level));
while(bb.length<"species subgroup".length()){bb.append(' ');}
bb.tab();
bb.append(results[level][CORRECT]).tab();
bb.append(results[level][INCORRECT_TAX_CORRECT_SSU]).tab();
bb.append(results[level][INCORRECT_TAX_MISSING_SSU]).tab();
bb.append(results[level][INCORRECT_TAX_INCORRECT_SSU]).tab();
bb.append(results[level][NOHIT]).nl();
if(bswAcc!=null){bswAcc.print(bb);}
}
}
private void printMap(ByteStreamWriter bsw){
if(verbose){System.err.println("printMap("+bsw+")");}
ByteBuilder bb=new ByteBuilder();
bb.append("#qID\trID\tANI\tAAI\n");
if(bsw!=null){bsw.print(bb);}
for(Entry<Long, Float> e : aniMap.entrySet()){
long key=e.getKey();
int qID=((int)(key>>>32));
int rID=(int)(key&Integer.MAX_VALUE);
float ani=e.getValue();
Float aai=aaiMap.get(key);
if(aai!=null){
bb.clear();
bb.append(qID).tab().append(rID).tab().append(ani, 3).tab().append(aai, 3).nl();
if(bsw!=null){bsw.print(bb);}
linesOut++;
bytesOut+=bb.length();
}
}
}
private void printResults(ResultLineParser parser, ByteStreamWriter bsw){
if(verbose){System.err.println("printResults("+bsw+")");}
ByteBuilder bb=new ByteBuilder();
bb.append("#Level \tRank\tANI_AVG\tSSU_AVG\tANI_STD\tSSU_STD\tSamples");
if(bsw!=null){bsw.println(bb);}
for(int level=0; level<taxLevels; level++){
long aniCount=parser.levelCounts[level];
long ssuCount=parser.levelCountsSSU[level];
if(aniCount>=minSamples && ((1L<<level)&printLevelsMask)!=0){
bb.clear();
String name=TaxTree.levelToStringExtended(level);
double aniSum=parser.levelAniSums[level];
double ssuSum=parser.levelSSUSums[level];
FloatList aniList=parser.aniLists[level];
FloatList ssuList=parser.ssuLists[level];
aniList.sort();
ssuList.sort();
double aniAvg=aniSum/aniCount;
double ssuAvg=ssuSum/ssuCount;
double aniStd=aniList.stdev();
double ssuStd=ssuList.stdev();
bb.append(name);
while(bb.length<9){bb.append(' ');}
bb.tab().append(level);
bb.tab().append(aniAvg, 3);
bb.tab().append(ssuAvg, 3);
bb.tab().append(aniStd, 3);
bb.tab().append(ssuStd, 3);
bb.tab().append(aniCount);
if(bsw!=null){bsw.println(bb);}
linesOut++;
bytesOut+=bb.length();
}
}
}
private static ByteStreamWriter makeBSW(FileFormat ff){
if(verbose){System.err.println("makeBSW("+ff+")");}
if(ff==null){return null;}
ByteStreamWriter bsw=new ByteStreamWriter(ff);
bsw.start();
return bsw;
}
private static long makePrintLevelsMask(String[] printLevelsArray){
long mask=0;
for(String s : printLevelsArray){
int level=TaxTree.stringToLevelExtended(s);
long bit=1L<<level;
assert(Long.bitCount(bit)==1);
mask|=bit;
}
return mask;
}
/*--------------------------------------------------------------*/
private class SSUThread extends Thread{
SSUThread(AtomicInteger atom_){
atom=atom_;
}
@Override
public void run(){
for(int idx=atom.getAndIncrement(); idx<recordSets.size(); idx=atom.getAndIncrement()){
RecordSet rs=recordSets.get(idx);
rs.sortAndSweep();
rs.processSSU();
}
}
private final AtomicInteger atom;
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
private String in1=null;
private String in2=null;
private String out1="stdout.txt";
private String outMap=null;
private String outAccuracy=null;
private String outBad=null;
private String treeFile=null;
private ByteStreamWriter bswBad=null;
/*--------------------------------------------------------------*/
private long linesProcessed=0;
private long linesOut=0;
private long bytesProcessed=0;
private long bytesOut=0;
private boolean shrinkOnly=false;
int minSamples=1;
private long maxLines=Long.MAX_VALUE;
final static int taxLevels=TaxTree.numTaxLevelNamesExtended;
final HashMap<Long, Float> aniMap=new HashMap<Long, Float>();
final HashMap<Long, Float> aaiMap=new HashMap<Long, Float>();
final TaxTree tree;
final ArrayList<RecordSet> recordSets;
// static HashMap<Integer, byte[]> ssuMap;
static final String[] printLevels=new String[] {"strain", "species", "genus", "family", "order", "class", "phylum", "superkingdom", "life"};
static final long printLevelsMask=makePrintLevelsMask(printLevels);
/*--------------------------------------------------------------*/
static int NOHIT=0;
static int CORRECT=1;
static int INCORRECT_TAX=2;
static int INCORRECT_SSU=4;
static int MISSING_SSU=8;
private static int INCORRECT_TAX_CORRECT_SSU=INCORRECT_TAX;
private static int INCORRECT_TAX_INCORRECT_SSU=INCORRECT_TAX|INCORRECT_SSU;
private static int INCORRECT_TAX_MISSING_SSU=INCORRECT_TAX|MISSING_SSU;
static final int BBSKETCH_MODE=0;
static final int MASH_MODE=1;
static final int SOURMASH_MODE=2;
static final int BLAST_MODE=3;
static int mode=BBSKETCH_MODE;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
private final FileFormat ffin1;
private final FileFormat ffin2;
private final FileFormat ffout1;
private final FileFormat ffoutMap;
private final FileFormat ffoutAccuracy;
private final FileFormat ffoutBad;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
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