1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316
|
package sketch;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import dna.AminoAcid;
import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.ConcurrentReadInputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import structures.LongHashSet;
import tracker.ReadStats;
/**
* @author Brian Bushnell
* @date Oct 17, 2014
*
*/
public class InvertKey extends SketchObject {
public static void main(String[] args){
Timer t=new Timer();
InvertKey x=new InvertKey(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public InvertKey(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
FASTQ.TEST_INTERLEAVED=FASTQ.FORCE_INTERLEAVED=false;
int k_=32, k2_=0;
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
ByteFile2.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
ConcurrentGenericReadInputStream.verbose=verbose;
stream.FastqReadInputStream.verbose=verbose;
ReadWrite.verbose=verbose;
}else if(a.equals("key")){
keyString=b;
}else if(a.equals("out")){
out1=b;
}else if(a.equalsIgnoreCase("k")){
assert(b!=null) : "Bad parameter: "+arg;
if(b.indexOf(',')>=0){
String[] bsplit=b.split(",");
assert(bsplit.length==2) : "Bad argument "+arg;
int x=Integer.parseInt(bsplit[0]);
int y=Integer.parseInt(bsplit[1]);
k_=Tools.max(x, y);
k2_=Tools.min(x, y);
if(k_==k2_){k2_=0;}
}else{
k_=Integer.parseInt(b);
k2_=0;
}
}else if(a.equalsIgnoreCase("printonce")){
printOnce=Parse.parseBoolean(b);
}else if(parser.in1==null && i==0 && Tools.looksLikeInputStream(arg)){
parser.in1=arg;
}else if(parser.out1==null && i==1 && !arg.contains("=")){
out1=arg;
}else if(parser.parse(arg, a, b)){
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
k=k_;
k2=k2_;
shift=2*k;
shift2=shift-2;
mask=(shift>63 ? -1L : ~((-1L)<<shift)); //Conditional allows K=32
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
in1=parser.in1;
}
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2){
ByteFile.FORCE_MODE_BF2=false;
ByteFile.FORCE_MODE_BF1=true;
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+"\n");
}
ffout1=FileFormat.testOutput(out1, FileFormat.FASTA, null, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTA, null, true, true);
SketchObject.postParse();
if(keyString.indexOf(',')>0){
String[] split=keyString.split(",");
set=new LongHashSet(split.length*2);
for(String s : split){
long x=Long.MAX_VALUE-Sketch.parseA48(s);
set.add(x);
// assert(set.contains(x)) : x+", "+set.size()+", "+set.toStringListView();
}
key0=-1;
// System.err.println(set.toStringListView()+", "+set.size());
assert(!set.isEmpty());
}else if(keyString.endsWith(".sketch")){
SketchTool tool=new SketchTool(10000, 0, false, false, rcomp);
Sketch sk=tool.loadSketchesFromFile(keyString, null, 0, 1000000, SketchObject.ONE_SKETCH, 1f, 0f, 0f, (byte)0, false).get(0);
set=new LongHashSet(sk.length()*2);
for(long x : sk.keys){set.add(Long.MAX_VALUE-x);}
key0=-1;
// System.err.println(set.toStringListView()+", "+set.size());
assert(!set.isEmpty());
}else{
key0=Long.MAX_VALUE-Sketch.parseA48(keyString);
set=null;
// System.err.println(key0);
}
}
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, null, null, null);
cris.start();
if(verbose){outstream.println("Started cris");}
}
boolean paired=cris.paired();
// if(verbose){
if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
// }
final ByteStreamWriter bsw;
if(out1!=null){
fasta=ffout1.fasta() && !out1.endsWith(".txt");
bsw=new ByteStreamWriter(ffout1);
bsw.start();
}else{bsw=null;}
long readsProcessed=0;
long basesProcessed=0;
boolean finished=false;
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
// outstream.println("Fetched "+reads);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
while(reads!=null && reads.size()>0 && !finished){
for(int idx=0; idx<reads.size() && !finished; idx++){
final Read r1=reads.get(idx);
finished=invert(key0, r1, bsw);
final int initialLength1=r1.length();
readsProcessed++;
basesProcessed+=initialLength1;
}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
errorState|=(ReadWrite.closeStream(cris));
if(bsw!=null){errorState|=bsw.poisonAndWait();}
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
if(errorState && !finished && maxReads<1){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
private boolean invert(long key2, Read r, ByteStreamWriter bsw) {
final byte[] bases=r.bases;
long kmer=0;
long rkmer=0;
int len=0;
// System.err.println("Looking for "+key+"\t"+Sketch.toA48(key)+"\t"+Sketch.toA48(Long.MAX_VALUE-key));
for(int i=0; i<bases.length; i++){
byte b=bases[i];
long x=AminoAcid.baseToNumber[b];
long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){len=0; rkmer=0;}else{len++;}
if(len>=k){
kmersProcessed++;
final long hashcode=hash(kmer, rkmer);
boolean found=(key0>=0 ? hashcode==key0 : set.contains(hashcode));
if(found){
if(fasta){bsw.println(">"+Sketch.toA48(Long.MAX_VALUE-hashcode)+" "+(i-k+1)+" "+r.id);}
bsw.println(AminoAcid.kmerToString(Tools.min(kmer, rkmer), k));
if(printOnce){
if(key0>=0){return true;}
else{
set.remove(hashcode);
return set.isEmpty();
}
}
}
}
}
return false;
}
/*--------------------------------------------------------------*/
final long key0;
final LongHashSet set;
final int shift;
final int shift2;
final long mask;
boolean printOnce=true;
long kmersProcessed=0;
private String in1=null;
boolean fasta;
boolean sketch;
private String keyString=null;
private String out1="stdout.fa";
/*--------------------------------------------------------------*/
private long maxReads=-1;
/*--------------------------------------------------------------*/
private final FileFormat ffin1;
private final FileFormat ffout1;
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
|