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package ukmer;
import java.io.File;
import java.util.ArrayList;
import java.util.BitSet;
import java.util.concurrent.atomic.AtomicLong;
import assemble.Contig;
import bloom.KmerCountAbstract;
import dna.AminoAcid;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import jgi.BBMerge;
import kmer.AbstractKmerTableSet;
import kmer.DumpThread;
import kmer.ScheduleMaker;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Primes;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import shared.TrimRead;
import stream.ConcurrentReadInputStream;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.IntList;
import structures.ListNum;
import tracker.ReadStats;
/**
* Loads and holds kmers for Tadpole2/KmerCountExact
* @author Brian Bushnell
* @date Jun 22, 2015
*
*/
public class KmerTableSetU extends AbstractKmerTableSet {
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer(), t2=new Timer();
t.start();
t2.start();
//Create a new CountKmersExact instance
KmerTableSetU set=new KmerTableSetU(args);
t2.stop();
outstream.println("Initialization Time: \t"+t2);
///And run it
set.process(t);
//Close the print stream if it was redirected
Shared.closeStream(outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
private KmerTableSetU(String[] args){
this(args, 0);//+5 if using ownership and building contigs
}
/**
* Constructor.
* @param args Command line arguments
*/
public KmerTableSetU(String[] args, int extraBytesPerKmer_){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}//TODO - no easy way to close outstream
/* Initialize local variables with defaults */
Parser parser=new Parser();
boolean prealloc_=false;
int kbig_=62;
int ways_=-1;
int filterMax_=2;
boolean ecco_=false, merge_=false;
boolean rcomp_=true;
double minProb_=defaultMinprob;
/* Parse arguments */
for(int i=0; i<args.length; i++){
final String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(parser.parseInterleaved(arg, a, b)){
//do nothing
}else if(parser.parseTrim(arg, a, b)){
//do nothing
}else if(a.equals("in") || a.equals("in1")){
in1.clear();
if(b!=null){
String[] s=b.split(",");
for(String ss : s){
in1.add(ss);
}
}
}else if(a.equals("in2")){
in2.clear();
if(b!=null){
String[] s=b.split(",");
for(String ss : s){
in2.add(ss);
}
}
}else if(a.equals("extra")){
extra.clear();
if(b!=null){
String[] s=b.split(",");
for(String ss : s){
extra.add(ss);
}
}
}else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
}else if(a.equals("initialsize")){
initialSize=Parse.parseIntKMG(b);
}else if(a.equals("showstats") || a.equals("stats")){
showStats=Parse.parseBoolean(b);
}else if(a.equals("ways")){
ways_=Parse.parseIntKMG(b);
}else if(a.equals("buflen") || a.equals("bufflen") || a.equals("bufferlength")){
buflen=Parse.parseIntKMG(b);
}else if(a.equals("k")){
assert(b!=null) : "\nk needs an integer value such as k=50. Default is 62.\n";
kbig_=Parse.parseIntKMG(b);
}else if(a.equals("threads") || a.equals("t")){
THREADS=(b==null || b.equalsIgnoreCase("auto") ? Shared.threads() : Integer.parseInt(b));
}else if(a.equals("showspeed") || a.equals("ss")){
showSpeed=Parse.parseBoolean(b);
}else if(a.equals("ecco")){
ecco_=Parse.parseBoolean(b);
}else if(a.equals("merge")){
merge_=Parse.parseBoolean(b);
}else if(a.equals("verbose")){
// assert(false) : "Verbose flag is currently static final; must be recompiled to change.";
verbose=Parse.parseBoolean(b);
}else if(a.equals("verbose2")){
// assert(false) : "Verbose flag is currently static final; must be recompiled to change.";
verbose2=Parse.parseBoolean(b);
}else if(a.equals("minprob")){
minProb_=Double.parseDouble(b);
}else if(a.equals("minprobprefilter") || a.equals("mpp")){
minProbPrefilter=Parse.parseBoolean(b);
}else if(a.equals("minprobmain") || a.equals("mpm")){
minProbMain=Parse.parseBoolean(b);
}else if(a.equals("reads") || a.startsWith("maxreads")){
maxReads=Parse.parseKMG(b);
}else if(a.equals("prealloc") || a.equals("preallocate")){
if(b==null || b.length()<1 || Character.isLetter(b.charAt(0))){
prealloc_=Parse.parseBoolean(b);
}else{
preallocFraction=Tools.max(0, Double.parseDouble(b));
prealloc_=(preallocFraction>0);
}
}else if(a.equals("prefilter")){
if(b==null || b.length()<1 || !Tools.isDigit(b.charAt(0))){
prefilter=Parse.parseBoolean(b);
}else{
filterMax_=Parse.parseIntKMG(b);
prefilter=filterMax_>0;
}
}else if(a.equals("prefiltersize") || a.equals("prefilterfraction") || a.equals("pff")){
prefilterFraction=Tools.max(0, Double.parseDouble(b));
assert(prefilterFraction<=1) : "prefiltersize must be 0-1, a fraction of total memory.";
prefilter=prefilterFraction>0;
}else if(a.equals("prehashes") || a.equals("hashes")){
prehashes=Parse.parseIntKMG(b);
}else if(a.equals("prefilterpasses") || a.equals("prepasses")){
assert(b!=null) : "Bad parameter: "+arg;
if(b.equalsIgnoreCase("auto")){
prepasses=-1;
}else{
prepasses=Parse.parseIntKMG(b);
}
}else if(a.equals("onepass")){
onePass=Parse.parseBoolean(b);
}else if(a.equals("passes")){
int passes=Parse.parseIntKMG(b);
onePass=(passes<2);
}else if(a.equals("rcomp")){
rcomp_=Parse.parseBoolean(b);
}
else if(a.equalsIgnoreCase("filterMemoryOverride") || a.equalsIgnoreCase("filterMemory") ||
a.equalsIgnoreCase("prefilterMemory") || a.equalsIgnoreCase("filtermem")){
filterMemoryOverride=Parse.parseKMG(b);
}
else if(IGNORE_UNKNOWN_ARGS){
//Do nothing
}else{
throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
qtrimLeft=parser.qtrimLeft;
qtrimRight=parser.qtrimRight;
trimq=parser.trimq;
trimE=parser.trimE();
minAvgQuality=parser.minAvgQuality;
minAvgQualityBases=parser.minAvgQualityBases;
}
if(prepasses==0 || !prefilter){
prepasses=0;
prefilter=false;
}
{
long memory=Runtime.getRuntime().maxMemory();
double xmsRatio=Shared.xmsRatio();
// long tmemory=Runtime.getRuntime().totalMemory();
usableMemory=(long)Tools.max(((memory-96000000)*(xmsRatio>0.97 ? 0.82 : 0.72)), memory*0.45);
if(prepasses==0 || !prefilter){
filterMemory0=filterMemory1=0;
}else if(filterMemoryOverride>0){
filterMemory0=filterMemory1=filterMemoryOverride;
}else{
double low=Tools.min(prefilterFraction, 1-prefilterFraction);
double high=1-low;
if(prepasses<0 || (prepasses&1)==1){//odd passes
filterMemory0=(long)(usableMemory*low);
filterMemory1=(long)(usableMemory*high);
}else{//even passes
filterMemory0=(long)(usableMemory*high);
filterMemory1=(long)(usableMemory*low);
}
}
tableMemory=(long)(usableMemory*.95-filterMemory0);
}
mult=Kmer.getMult(kbig_);
k=Kmer.getK(kbig_);
kbig=k*mult;
kbig2=kbig-1;
assert(k<=31);
prealloc=prealloc_;
bytesPerKmer=4+8*mult+extraBytesPerKmer_;
assert(bytesPerKmer>=4+8*mult) : bytesPerKmer+", "+mult+", "+k+", "+kbig+", "+(4+8*mult)+", "+extraBytesPerKmer_;
if(ways_<1){
long maxKmers=(2*tableMemory)/bytesPerKmer;
long minWays=Tools.min(10000, maxKmers/Integer.MAX_VALUE);
ways_=(int)Tools.max(31, (int)(Tools.min(96, Shared.threads())*2.5), minWays);
ways_=(int)Primes.primeAtLeast(ways_);
assert(ways_>0);
// System.err.println("ways="+ways_);
}
// assert(false) : extraBytesPerKmer_+bytesPerKmer+", "+mult+", "+k+", "+kbig+", "+(4+8*mult)+", "+ways_;
/* Set final variables; post-process and validate argument combinations */
onePass=onePass&prefilter;
ways=ways_;
filterMax=Tools.min(filterMax_, 0x7FFFFFFF);
ecco=ecco_;
merge=merge_;
minProb=(float)minProb_;
rcomp=rcomp_;
Kmer.rcomp=rcomp;
// System.err.println("rcomp="+rcomp);
estimatedKmerCapacity=(long)((tableMemory*1.0/bytesPerKmer)*((prealloc ? preallocFraction : 0.81))*(HashArrayU.maxLoadFactorFinal*0.97));
KmerCountAbstract.minProb=(minProbPrefilter ? minProb : 0);
if(kbig!=kbig_){
System.err.println("K was changed from "+kbig_+" to "+kbig);
}
if(k<1){throw new RuntimeException("\nk needs an integer value above 0, such as k=27. Default is 62.\n");}
if(initialSize<1){
final long memOverWays=tableMemory/(bytesPerKmer*ways);
final double mem2=(prealloc ? preallocFraction : 1)*tableMemory;
initialSize=(prealloc || memOverWays<initialSizeDefault ? (int)Tools.min(2142000000, (long)(mem2/(bytesPerKmer*ways))) : initialSizeDefault);
if(initialSize!=initialSizeDefault){
System.err.println("Initial size set to "+initialSize);
}
}
/* Adjust I/O settings and filenames */
assert(FastaReadInputStream.settingsOK());
if(in1.isEmpty()){throw new RuntimeException("Error - at least one input file is required.");}
for(int i=0; i<in1.size(); i++){
String s=in1.get(i);
if(s!=null && s.contains("#") && !new File(s).exists()){
int pound=s.lastIndexOf('#');
String a=s.substring(0, pound);
String b=s.substring(pound+1);
in1.set(i, a+1+b);
in2.add(a+2+b);
}
}
if(!extra.isEmpty()){
ArrayList<String> temp=(ArrayList<String>) extra.clone();
extra.clear();
for(String s : temp){
if(s!=null && s.contains("#") && !new File(s).exists()){
int pound=s.lastIndexOf('#');
String a=s.substring(0, pound);
String b=s.substring(pound+1);
extra.add(a);
extra.add(b);
}else{
extra.add(s);
}
}
}
{
boolean allowDuplicates=true;
if(!Tools.testInputFiles(allowDuplicates, true, in1, in2, extra)){
throw new RuntimeException("\nCan't read some input files.\n");
}
}
assert(THREADS>0);
if(DISPLAY_PROGRESS){
outstream.println("Initial:");
outstream.println("Ways="+ways+", initialSize="+initialSize+", prefilter="+(prefilter ? "t" : "f")+", prealloc="+(prealloc ? (""+preallocFraction) : "f"));
Shared.printMemory();
outstream.println();
}
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
public void clear(){
tables=null;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
protected void allocateTables(){
assert(tables==null);
tables=null;
final int tableType=AbstractKmerTableU.ARRAY1D;
ScheduleMaker scheduleMaker=new ScheduleMaker(ways, bytesPerKmer, prealloc,
(prealloc ? preallocFraction : 1.0), -1, (prefilter ? prepasses : 0), prefilterFraction, filterMemoryOverride);
int[] schedule=scheduleMaker.makeSchedule();
tables=AbstractKmerTableU.preallocate(ways, tableType, schedule, k, kbig);
}
/**
* Load reads into tables, using multiple LoadThread.
*/
@Override
public long loadKmers(String fname1, String fname2){
/* Create read input stream */
final ConcurrentReadInputStream cris;
{
FileFormat ff1=FileFormat.testInput(fname1, FileFormat.FASTQ, null, true, true);
FileFormat ff2=FileFormat.testInput(fname2, FileFormat.FASTQ, null, true, true);
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, false, ff1, ff2);
cris.start(); //4567
}
/* Create ProcessThreads */
ArrayList<LoadThread> alpt=new ArrayList<LoadThread>(THREADS);
for(int i=0; i<THREADS; i++){alpt.add(new LoadThread(cris));}
for(LoadThread pt : alpt){pt.start();}
long added=0;
/* Wait for threads to die, and gather statistics */
for(LoadThread pt : alpt){
while(pt.getState()!=Thread.State.TERMINATED){
try {
pt.join();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
added+=pt.added;
readsIn+=pt.readsInT;
basesIn+=pt.basesInT;
lowqReads+=pt.lowqReadsT;
lowqBases+=pt.lowqBasesT;
readsTrimmed+=pt.readsTrimmedT;
basesTrimmed+=pt.basesTrimmedT;
kmersIn+=pt.kmersInT;
}
/* Shut down I/O streams; capture error status */
errorState|=ReadWrite.closeStreams(cris);
return added;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/**
* Loads kmers.
*/
private class LoadThread extends Thread{
/**
* Constructor
* @param cris_ Read input stream
*/
public LoadThread(ConcurrentReadInputStream cris_){
cris=cris_;
table=new HashBufferU(tables, buflen, kbig, false);
kmer=new Kmer(k, mult);
}
@Override
public void run(){
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
//While there are more reads lists...
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
//For each read (or pair) in the list...
for(int i=0; i<reads.size(); i++){
Read r1=reads.get(i);
Read r2=r1.mate;
if(!r1.validated()){r1.validate(true);}
if(r2!=null && !r2.validated()){r2.validate(true);}
if(verbose){System.err.println("Considering read "+r1.id+" "+new String(r1.bases));}
readsInT++;
basesInT+=r1.length();
if(r2!=null){
readsInT++;
basesInT+=r2.length();
}
//Determine whether to discard the reads based on average quality
if(minAvgQuality>0){
if(r1!=null && r1.quality!=null && r1.avgQuality(false, minAvgQualityBases)<minAvgQuality){r1.setDiscarded(true);}
if(r2!=null && r2.quality!=null && r2.avgQuality(false, minAvgQualityBases)<minAvgQuality){r2.setDiscarded(true);}
}
if(r1!=null){
if(qtrimLeft || qtrimRight){
int x=TrimRead.trimFast(r1, qtrimLeft, qtrimRight, trimq, trimE, 1);
basesTrimmedT+=x;
readsTrimmedT+=(x>0 ? 1 : 0);
}
if(r1.length()<kbig){r1.setDiscarded(true);}
}
if(r2!=null){
if(qtrimLeft || qtrimRight){
int x=TrimRead.trimFast(r2, qtrimLeft, qtrimRight, trimq, trimE, 1);
basesTrimmedT+=x;
readsTrimmedT+=(x>0 ? 1 : 0);
}
if(r2.length()<kbig){r2.setDiscarded(true);}
}
if((ecco || merge) && r1!=null && r2!=null && !r1.discarded() && !r2.discarded()){
if(merge){
final int insert=BBMerge.findOverlapStrict(r1, r2, false);
if(insert>0){
r2.reverseComplement();
r1=r1.joinRead(insert);
r2=null;
}
}else if(ecco){
BBMerge.findOverlapStrict(r1, r2, true);
}
}
if(r1!=null){
if(r1.discarded()){
lowqBasesT+=r1.length();
lowqReadsT++;
}else{
long temp=addKmersToTable(r1, kmer);
added+=temp;
if(verbose){System.err.println("A: Added "+temp);}
}
}
if(r2!=null){
if(r2.discarded()){
lowqBasesT+=r2.length();
lowqReadsT++;
}else{
long temp=addKmersToTable(r2, kmer);
added+=temp;
if(verbose){System.err.println("B: Added "+temp);}
}
}
}
//Fetch a new read list
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
cris.returnList(ln);
long temp=table.flush();
if(verbose){System.err.println("Flush: Added "+temp);}
added+=temp;
}
private final int addKmersToTable(final Read r, Kmer kmer){
if(onePass){return addKmersToTable_onePass(r, kmer);}
if(r==null || r.bases==null){return 0;}
final float minProb2=(minProbMain ? minProb : 0);
final byte[] bases=r.bases;
final byte[] quals=r.quality;
int created=0;
int len=0;
if(bases==null || bases.length<kbig){return -1;}
kmer.clear();
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts */
float prob=1;
for(int i=0; i<bases.length; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
// assert(x>=0) : ((char)b)+", "+x+", "+new String(bases);
//Update kmers
// kmer.addRight(b);
kmer.addRightNumeric(x);
if(minProb2>0 && quals!=null){//Update probability
prob=prob*PROB_CORRECT[quals[i]];
if(len>kbig){
byte oldq=quals[i-kbig];
prob=prob*PROB_CORRECT_INVERSE[oldq];
}
}
//Handle Ns
if(x<0){
len=0;
prob=1;
}else{len++;}
assert(len==kmer.len);
// if(verbose){System.err.println("A: Scanning i="+i+", len="+len+", kmer="+kmer.toString()+"\t"+new String(bases, Tools.max(0, i-kbig2), Tools.min(i+1, kbig)));}
if(len>=kbig && prob>=minProb2){
kmersInT++;
// System.err.println("kmer="+kmer+"; xor()="+kmer.xor()+"; filterMax2="+filterMax2+"; prefilter="+prefilter);
// System.err.println("prefilterArray.read(xor.key())="+prefilterArray.read(kmer.xor())+"");
// System.err.println("prefilterArray.read(kmer.key())="+prefilterArray.read(kmer.key())+"");
if(!prefilter || prefilterArray.read(kmer.xor())>filterMax2){
int temp=table.incrementAndReturnNumCreated(kmer);
// System.err.println("kmer="+kmer+"; xor()="+kmer.xor()+"; temp="+temp+" ");
created+=temp;
if(verbose){System.err.println("C: Added "+temp);}
}
}
}
return created;
}
private final int addKmersToTable_onePass(final Read r, Kmer kmer){
assert(prefilter);
if(r==null || r.bases==null){return 0;}
final byte[] bases=r.bases;
final byte[] quals=r.quality;
int created=0;
int len=0;
if(bases==null || bases.length<kbig){return -1;}
kmer.clear();
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts */
float prob=1;
for(int i=0; i<bases.length; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
//Update kmers
kmer.addRight(b);
if(minProb>0 && quals!=null){//Update probability
prob=prob*PROB_CORRECT[quals[i]];
if(len>kbig){
byte oldq=quals[i-kbig];
prob=prob*PROB_CORRECT_INVERSE[oldq];
}
}
//Handle Ns
if(x<0){
len=0;
prob=1;
}else{len++;}
assert(len==kmer.len);
if(verbose){System.err.println("B: Scanning i="+i+", len="+len+", kmer="+kmer+"\t"+new String(bases, Tools.max(0, i-kbig2), Tools.min(i+1, kbig)));}
if(len>=kbig && prob>=minProb){
final long xor=kmer.xor();
int count=prefilterArray.incrementAndReturnUnincremented(xor, 1);
if(count>=filterMax2){
int temp=table.incrementAndReturnNumCreated(kmer);
created+=temp;
if(verbose){System.err.println("D: Added "+temp);}
}
}
}
return created;
}
/*--------------------------------------------------------------*/
/** Input read stream */
private final ConcurrentReadInputStream cris;
private final HashBufferU table;
public long added=0;
private long readsInT=0;
private long basesInT=0;
private long lowqReadsT=0;
private long lowqBasesT=0;
private long readsTrimmedT=0;
private long basesTrimmedT=0;
private long kmersInT=0;
private final Kmer kmer;
}
/*--------------------------------------------------------------*/
/*---------------- Convenience ----------------*/
/*--------------------------------------------------------------*/
public int regenerateCounts(byte[] bases, IntList counts, final int ca, final Kmer kmer){
final int b=ca+kbig; //first base changed
final int lim=Tools.min(counts.size, ca+kbig+1); //count limit
// System.err.println("ca="+ca+", b="+b+", lim="+lim);
// System.err.println("Regen from count "+(ca+1)+"-"+lim);
int len=0;
int valid=0;
kmer.clear();
// System.err.println("ca="+ca+", b="+b+", lim="+lim+", "+counts);
//Generate initial kmer
for(int i=b-kbig; i<b; i++){
final byte base=bases[i];
final long x=AminoAcid.baseToNumber[base];
kmer.addRight(base);
if(x<0){
len=0;
}else{len++;}
assert(len==kmer.len);
}
assert(len==kbig || Tools.indexOf(bases, (byte)'N')>=ca) : new String(bases)+"\n"+ca+", "+len;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts.
* i is an index in the base array, j is an index in the count array. */
for(int i=b, j=ca+1; j<lim; i++, j++){
final byte base=bases[i];
final long x=AminoAcid.baseToNumber[base];
kmer.addRight(base);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(len>=kbig){
valid++;
int count=getCount(kmer);
counts.set(j, count);
}else{
counts.set(j, 0);
}
}
// System.err.println("ca="+ca+", b="+b+", lim="+lim+", "+counts);
return valid;
}
@Override
public int regenerateCounts(byte[] bases, IntList counts, final Kmer kmer, BitSet changed){
assert(!changed.isEmpty());
final int firstBase=changed.nextSetBit(0), lastBase=changed.length()-1;
final int ca=firstBase-kbig;
// final int b=changed.nextSetBit(0);ca+kbig; //first base changed
final int firstCount=Tools.max(firstBase-kbig+1, 0), lastCount=Tools.min(counts.size-1, lastBase); //count limit
// System.err.println("ca="+ca+", b="+b+", lim="+lim);
// System.err.println("Regen from count "+(ca+1)+"-"+lim);
int len=0;
int valid=0;
kmer.clear();
// System.err.println("ca="+ca+", b="+b+", lim="+lim+", "+counts);
//Generate initial kmer
for(int i=Tools.max(0, firstBase-kbig+1); i<firstBase; i++){
final byte base=bases[i];
final long x=AminoAcid.baseToNumber[base];
kmer.addRight(base);
if(x<0){
len=0;
}else{len++;}
assert(len==kmer.len);
}
assert(len==kbig-1 || Tools.indexOf(bases, (byte)'N')>=0 || firstBase<kbig) :
new String(bases)+"\n"+ca+", "+len+", "+firstBase;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts.
* i is an index in the base array, j is an index in the count array. */
for(int i=firstBase, lim=Tools.min(lastBase+k-1, bases.length-1); i<=lim; i++){
final byte base=bases[i];
final long x=AminoAcid.baseToNumber[base];
kmer.addRight(base);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
final int c=i-kbig+1;
if(i>=firstBase){
if(len>=kbig){
valid++;
int count=getCount(kmer);
counts.set(c, count);
}else if(c>=0){
counts.set(c, 0);
}
}
}
// System.err.println("ca="+ca+", b="+b+", lim="+lim+", "+counts);
return valid;
}
@Override
public int fillSpecificCounts(byte[] bases, IntList counts, BitSet positions, final Kmer kmer){
counts.clear();
{
Kmer x=leftmostKmer(bases, bases.length, kmer);
assert((x!=null)==(kmer.len==kbig));
}
int len=kmer.len;
int valid=0;
if(len>=kbig){
valid++;
int count=getCount(kmer);
counts.add(count);
}else{
counts.add(0);
}
assert(kmer.len==len);
assert(len<=kbig) : len+", "+kbig;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts */
for(int i=kbig, j=0; i<bases.length; i++, j++){
final byte base=bases[i];
final long x=AminoAcid.baseToNumber[base];
kmer.addRight(base);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(len>=kbig && (positions==null || positions.get(j))){
valid++;
int count=getCount(kmer);
counts.add(Tools.max(count, 0));
}else{
counts.add(0);
}
}
return valid;
}
/*--------------------------------------------------------------*/
/*---------------- Helper Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
public long regenerate(final int limit){
long sum=0;
for(AbstractKmerTableU akt : tables){
sum+=akt.regenerate(limit);
}
return sum;
}
public HashArrayU1D getTable(Kmer kmer){
return (HashArrayU1D) tables[kmer.mod(ways)];
}
@Override
public HashArrayU1D getTable(int tnum){
return (HashArrayU1D) tables[tnum];
}
@Override
public long[] fillHistogram(int histMax) {
return HistogramMakerU.fillHistogram(tables, histMax);
}
@Override
public void countGC(long[] gcCounts, int max) {
for(AbstractKmerTableU set : tables){
set.countGC(gcCounts, max);
}
}
@Override
public void initializeOwnership(){
OwnershipThread.initialize(tables);
}
@Override
public void clearOwnership(){
OwnershipThread.clear(tables);
}
public Kmer rightmostKmer(final ByteBuilder bb, Kmer kmer){
return rightmostKmer(bb.array, bb.length(), kmer);
}
public Kmer rightmostKmer(final byte[] bases, final int blen, final Kmer kmer){
kmer.clear();
if(blen<kbig){return null;}
int len=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
{
for(int i=blen-kbig; i<blen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
//if(verbose){outstream.println("C: Scanning i="+i+", len="+len+", kmer="+kmer+"\t"+new String(bases, Tools.max(0, i-kbig2), Tools.min(i+1, kbig)));}
}
}
if(len<kbig){return null;}
else{assert(len==kbig);}
return kmer;
}
public Kmer leftmostKmer(final ByteBuilder bb, final Kmer kmer){
return leftmostKmer(bb.array, bb.length(), kmer);
}
public Kmer leftmostKmer(final byte[] bases, final int blen, final Kmer kmer){
kmer.clear();
if(blen<kbig){return null;}
int len=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
{
for(int i=0; i<kbig; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(verbose){outstream.println("D: Scanning i="+i+", len="+len+", kmer="+kmer+"\t"+new String(bases, Tools.max(0, i-kbig2), Tools.min(i+1, kbig)));}
}
}
if(len<kbig){return null;}
else{assert(len==kbig);}
return kmer;
}
public boolean doubleClaim(final ByteBuilder bb, final int id, Kmer kmer){
return doubleClaim(bb.array, bb.length(), id, kmer);
}
/** Ensures there can be only one owner. */
public boolean doubleClaim(final byte[] bases, final int blength, final int id, Kmer kmer){
boolean success=claim(bases, blength, id, true, kmer);
if(verbose){outstream.println("success1="+success+", id="+id+", blength="+blength);}
if(!success){return false;}
success=claim(bases, blength, id+CLAIM_OFFSET, true, kmer);
if(verbose){outstream.println("success2="+success+", id="+id+", blength="+blength);}
return success;
}
public boolean claim(final ByteBuilder bb, final int id, final boolean exitEarly, Kmer kmer){
return claim(bb.array, bb.length(), id, exitEarly, kmer);
}
public float calcCoverage(final byte[] bases, final int blen, final Kmer kmer){
if(blen<kbig){return 0;}
int len=0;
kmer.clear();
long sum=0;
int kmers=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
for(int i=0; i<blen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(len>=kbig){
int count=getCount(kmer);
sum+=count;
kmers++;
}
}
return sum==0 ? 0 : sum/(float)kmers;
}
public float calcCoverage(final Contig contig, final Kmer kmer){
final byte[] bases=contig.bases;
if(bases.length<kbig){return 0;}
int len=0;
kmer.clear();
long sum=0;
int max=0, min=Integer.MAX_VALUE;
int kmers=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
for(int i=0; i<bases.length; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(len>=kbig){
int count=getCount(kmer);
sum+=count;
max=Tools.max(count, max);
min=Tools.min(count, min);
kmers++;
}
}
contig.coverage=sum==0 ? 0 : sum/(float)kmers;
contig.maxCov=max;
contig.minCov=sum==0 ? 0 : min;
return contig.coverage;
}
public boolean claim(final byte[] bases, final int blen, final int id, boolean exitEarly, final Kmer kmer){
if(blen<kbig){return false;}
if(verbose){outstream.println("Thread "+id+" claim start.");}
int len=0;
kmer.clear();
boolean success=true;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts */
for(int i=0; i<blen && success; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(len>=kbig){
success=claim(kmer, id/*, rid, i*/);
success=(success || !exitEarly);
}
}
return success;
}
public boolean claim(Kmer kmer, final int id/*, final long rid, final int pos*/){
//TODO: rid and pos are just for debugging.
final int way=kmer.mod(ways);
final AbstractKmerTableU table=tables[way];
final int count=table.getValue(kmer);
assert(count==-1 || count>0) : count;
// if(verbose /*|| true*/){outstream.println("Count="+count+".");}
if(count<0){return true;}
assert(count>0) : count;
final int owner=table.setOwner(kmer, id);
if(verbose){outstream.println("owner="+owner+".");}
// assert(owner==id) : id+", "+owner+", "+rid+", "+pos;
return owner==id;
}
public void release(ByteBuilder bb, final int id, final Kmer kmer){
release(bb.array, bb.length(), id, kmer);
}
public void release(final byte[] bases, final int blen, final int id, final Kmer kmer){
if(verbose /*|| true*/){outstream.println("*Thread "+id+" release start.");}
int len=0;
kmer.clear();
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts */
for(int i=0; i<blen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
if(len>=kbig){
release(kmer, id);
}
}
}
public boolean release(Kmer kmer, final int id){
final int way=kmer.mod(ways);
final AbstractKmerTableU table=tables[way];
final int count=table.getValue(kmer);
// if(verbose /*|| true*/){outstream.println("Count="+count+".");}
if(count<1){return true;}
return table.clearOwner(kmer, id);
}
public int findOwner(ByteBuilder bb, final int id, final Kmer kmer){
return findOwner(bb.array, bb.length(), id, kmer);
}
public int findOwner(final byte[] bases, final int blen, final int id, final Kmer kmer){
int len=0;
kmer.clear();
int maxOwner=-1;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts */
for(int i=0; i<blen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
kmer.addRight(b);
if(x<0){len=0;}
else{len++;}
assert(len==kmer.len);
//if(verbose){System.err.println("E: Scanning i="+i+", len="+len+", kmer="+kmer+"\t"+new String(bases, Tools.max(0, i-kbig2), Tools.min(i+1, kbig)));}
if(len>=kbig){
int owner=findOwner(kmer);
maxOwner=Tools.max(owner, maxOwner);
if(maxOwner>id){break;}
}
}
return maxOwner;
}
public int findOwner(final Kmer kmer){
final int way=kmer.mod(ways);
final AbstractKmerTableU table=tables[way];
final int count=table.getValue(kmer);
if(count<0){return -1;}
final int owner=table.getOwner(kmer);
return owner;
}
public int getCount(Kmer kmer){
int way=kmer.mod(ways);
return tables[way].getValue(kmer);
}
public int getOwner(Kmer kmer){
int way=kmer.mod(ways);
return tables[way].getOwner(kmer);
}
/*--------------------------------------------------------------*/
/*---------------- Fill Counts ----------------*/
/*--------------------------------------------------------------*/
public int fillRightCounts(Kmer kmer, int[] counts){
if(FAST_FILL && MASK_CORE && k>2){
return fillRightCounts_fast(kmer, counts);
}else{
return fillRightCounts_safe(kmer, counts);
}
}
public int fillLeftCounts(Kmer kmer, int[] counts){
if(FAST_FILL && MASK_CORE && k>2){
return fillLeftCounts_fast(kmer, counts);
}else{
return fillLeftCounts_safe(kmer, counts);
}
}
public int fillRightCounts_fast(Kmer kmer, int[] counts){
assert(MASK_CORE);
final long old=kmer.addRightNumeric(0);
if(kmer.corePalindrome()){
kmer.addLeftNumeric(old);
return fillRightCounts_safe(kmer, counts);
}
final int way=kmerToWay(kmer);
final HashArrayU table=(HashArrayU) tables[way];
final int cell=table.kmerToCell(kmer);
int max=-1, maxPos=0;
{//Iteration 0
final int count=Tools.max(0, table.getValue(kmer, cell));
assert(count==NOT_PRESENT || count>=0);
counts[0]=count;
max=count;
maxPos=0;
kmer.addLeftNumeric(old);
}
for(int i=1; i<=3; i++){
kmer.addRightNumeric(i);
if(verbose){outstream.println("kmer: "+kmer);}
final int count=Tools.max(0, table.getValue(kmer, cell));
assert(count==NOT_PRESENT || count>=0);
counts[i]=count;
if(count>max){
max=count;
maxPos=i;
}
kmer.addLeftNumeric(old);
}
return maxPos;
}
public int fillRightCounts_safe(Kmer kmer, int[] counts){
assert(kmer.len>=kbig);
if(verbose){outstream.println("fillRightCounts: "+kmer);}
int max=-1, maxPos=0;
// final Kmer kmer2=new Kmer(kmer);//123 TODO: Slow, for an assertion only;
for(int i=0; i<=3; i++){
final long old=kmer.addRightNumeric(i);
if(verbose){outstream.println("kmer: "+kmer);}
int way=kmer.mod(ways);
int count=tables[way].getValue(kmer);
assert(count==NOT_PRESENT || count>=0);
count=Tools.max(count, 0);
counts[i]=count;
if(count>max){
max=count;
maxPos=i;
}
kmer.addLeftNumeric(old);
}
return maxPos;
}
// public int fillLeftCounts_fast(Kmer kmer, int[] counts){
// assert(MASK_CORE);
// final long old=kmer.addLeftNumeric(0);
// if(kmer.corePalindrome()){
// kmer.addRightNumeric(old);
// return fillLeftCounts_safe(kmer, counts);
// }
// final int way=kmerToWay(kmer);
// final HashArrayU table=(HashArrayU) tables[way];
// final int cell=table.kmerToCell(kmer);
// int max=-1, maxPos=0;
//
// for(int i=0; i<=3; i++){
// kmer.addLeftNumeric(i);
// if(verbose){outstream.println("kmer: "+kmer);}
// final int count=Tools.max(0, table.getValue(kmer, cell));
// assert(count==NOT_PRESENT || count>=0);
// counts[i]=count;
// if(count>max){
// max=count;
// maxPos=i;
// }
// kmer.addRightNumeric(old);
// }
// return maxPos;
// }
public int fillLeftCounts_fast(Kmer kmer, int[] counts){
assert(MASK_CORE);
final long old=kmer.addLeftNumeric(0);
if(kmer.corePalindrome()){
kmer.addRightNumeric(old);
return fillLeftCounts_safe(kmer, counts);
}
final int way=kmerToWay(kmer);
final HashArrayU table=(HashArrayU) tables[way];
final int cell=table.kmerToCell(kmer);
int max=-1, maxPos=0;
{//Iteration 0
if(verbose){outstream.println("kmer: "+kmer);}
final int count=Tools.max(0, table.getValue(kmer, cell));
assert(count==NOT_PRESENT || count>=0);
counts[0]=count;
max=count;
maxPos=0;
kmer.addRightNumeric(old);
}
for(int i=1; i<=3; i++){
kmer.addLeftNumeric(i);
if(verbose){outstream.println("kmer: "+kmer);}
final int count=Tools.max(0, table.getValue(kmer, cell));
assert(count==NOT_PRESENT || count>=0);
counts[i]=count;
if(count>max){
max=count;
maxPos=i;
}
kmer.addRightNumeric(old);
}
return maxPos;
}
public int fillLeftCounts_safe(final Kmer kmer, int[] counts){
assert(kmer.len>=kbig);
if(verbose){outstream.println("fillLeftCounts: "+kmer);}
int max=-1, maxPos=0;
// final Kmer kmer2=new Kmer(kmer);//123 TODO: Slow, for an assertion only;
// assert(false) : kmer+", "+kmer2;
for(int i=0; i<=3; i++){
if(verbose){
outstream.println("kmer: "+kmer+" (key==array1 ? "+(kmer.key()==kmer.array1()));
// outstream.println("kmer2: "+kmer2);
}
final long old=kmer.addLeftNumeric(i);
if(verbose){
outstream.println("after: "+kmer+" (key==array1 ? "+(kmer.key()==kmer.array1()));
outstream.println("i="+i+", old="+old);
}
int way=kmer.mod(ways);
int count=tables[way].getValue(kmer);
assert(count==NOT_PRESENT || count>=0);
count=Tools.max(count, 0);
counts[i]=count;
if(count>max){
max=count;
maxPos=i;
}
kmer.addRightNumeric(old);
if(verbose){outstream.println("restored: "+kmer);}
// assert(kmer.equals(kmer2)) : kmer+", "+kmer2+", "+kmer.xor()+", "+kmer2.xor();
}
return maxPos;
}
/*--------------------------------------------------------------*/
/*---------------- Printing Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
public boolean dumpKmersAsBytes(String fname, int mincount, int maxcount, boolean printTime, AtomicLong remaining){
if(fname==null){return false;}
Timer t=new Timer();
ByteStreamWriter bsw=new ByteStreamWriter(fname, overwrite, append, true);
bsw.start();
for(AbstractKmerTableU set : tables){
set.dumpKmersAsBytes(bsw, k, mincount, maxcount, remaining);
}
bsw.poisonAndWait();
t.stop();
if(printTime){outstream.println("Kmer Dump Time: \t"+t);}
return bsw.errorState;
}
@Override
public boolean dumpKmersAsBytes_MT(String fname, int mincount, int maxcount, boolean printTime, AtomicLong remaining){
final int threads=Tools.min(Shared.threads(), tables.length);
if(threads<3 || DumpThread.NUM_THREADS==1){return dumpKmersAsBytes(fname, mincount, maxcount, printTime, remaining);}
if(fname==null){return false;}
Timer t=new Timer();
ByteStreamWriter bsw=new ByteStreamWriter(fname, overwrite, append, true);
bsw.start();
DumpThreadU.dump(k, mincount, maxcount, tables, bsw, remaining);
bsw.poisonAndWait();
t.stop();
if(printTime){outstream.println("Kmer Dump Time: \t"+t);}
return bsw.errorState;
}
/*--------------------------------------------------------------*/
/*---------------- Recall Methods ----------------*/
/*--------------------------------------------------------------*/
private final StringBuilder toText(long[] kmer){return AbstractKmerTableU.toText(kmer, k);}
/*--------------------------------------------------------------*/
/*---------------- Final Primitives ----------------*/
/*--------------------------------------------------------------*/
@Override
public int kbig(){return kbig;}
@Override
public long filterMemory(int pass){return ((pass&1)==0) ? filterMemory0 : filterMemory1;}
@Override
public boolean ecco(){return ecco;}
@Override
public boolean qtrimLeft(){return qtrimLeft;}
@Override
public boolean qtrimRight(){return qtrimRight;}
@Override
public float minAvgQuality(){return minAvgQuality;}
@Override
public long tableMemory(){return tableMemory;}
@Override
public long estimatedKmerCapacity(){return estimatedKmerCapacity;}
@Override
public int ways(){return ways;}
@Override
public boolean rcomp(){return rcomp;}
public final int kmerToWay(final Kmer kmer){
final int way=(int)(kmer.xor()%ways);
return way;
}
/** Hold kmers. A kmer X such that X%WAYS=Y will be stored in tables[Y] */
private AbstractKmerTableU[] tables;
public AbstractKmerTableU[] tables(){return tables;}
public long filterMemoryOverride=0;
private final int bytesPerKmer;
private final long usableMemory;
private final long filterMemory0;
private final long filterMemory1;
private final long tableMemory;
private final long estimatedKmerCapacity;
/** Number of tables (and threads, during loading) */
private final boolean prealloc;
/** Number of tables (and threads, during loading) */
public final int ways;
/** Total kmer length */
public final int kbig;
/** Normal kmer length */
public final int k;
/** kbig-1; used in some expressions */
public final int kbig2;
/** Number of little kmers in a big kmer */
public final int mult;
/** Look for reverse-complements as well as forward kmers. Default: true */
private final boolean rcomp;
/** Quality-trim the left side */
public final boolean qtrimLeft;
/** Quality-trim the right side */
public final boolean qtrimRight;
/** Trim bases at this quality or below. Default: 4 */
public final float trimq;
/** Error rate for trimming (derived from trimq) */
private final float trimE;
/** Throw away reads below this average quality after trimming. Default: 0 */
public final float minAvgQuality;
/** If positive, calculate average quality from the first X bases only. Default: 0 */
public final int minAvgQualityBases;
/** Ignore kmers with probability of correctness less than this */
public final float minProb;
/** Correct via overlap */
private final boolean ecco;
/** Attempt to merge via overlap prior to counting kmers */
private final boolean merge;
/*--------------------------------------------------------------*/
/*---------------- Walker ----------------*/
/*--------------------------------------------------------------*/
public WalkerU walk(){
return new WalkerKSTU();
}
public class WalkerKSTU extends WalkerU {
WalkerKSTU(){
w=tables[0].walk();
}
/**
* Fills this object with the next key and value.
* @return True if successful.
*/
public boolean next(){
if(w==null){return false;}
if(w.next()){return true;}
if(tnum<tables.length){tnum++;}
w=(tnum<tables.length ? tables[tnum].walk() : null);
return w==null ? false : w.next();
}
public Kmer kmer(){return w.kmer();}
public int value(){return w.value();}
private WalkerU w=null;
/** current table number */
private int tnum=0;
}
}
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