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package aligner;
import java.util.ArrayList;
import dna.AminoAcid;
import dna.Data;
import fileIO.FileFormat;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.Read;
import stream.SamHeader;
import structures.LongHashMap;
/**
* Index for a MicroAligner.
*
* @author Brian Bushnell
* @date November 15, 2024
*
*/
public class MicroIndex3 {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
public MicroIndex3(int k_, int midMaskLen_, String path, boolean setSamStatics) {
this(k_, midMaskLen_, loadRef(path, setSamStatics));
}
public MicroIndex3(int k_, int midMaskLen_, Read r) {
// this(k_, minIdentity_, ref_.length);
ref=r.bases;
refname=r.id;
k=k_;
k2=k-1;
midMaskLen=midMaskLen_;
middleMask=makeMidMask(k, midMaskLen);
map=new LongHashMap(ref.length*2);
index();
assert(map.size()>0) : ref.length+", "+k;
}
public static long makeMidMask(int k, int midMaskLen) {
assert(k>midMaskLen+1);
int bitsPerBase=2;
int bits=midMaskLen*bitsPerBase;
int shift=((k-midMaskLen)/2)*bitsPerBase;
long middleMask=~((~((-1L)<<bits))<<shift);
return middleMask;
}
public static Read loadRef(String path, boolean setSamStatics) {
ArrayList<Read> list=ConcurrentGenericReadInputStream.getReads(1, false,
FileFormat.testInput(path, null, false), null, null, null);
// assert(false && !list.isEmpty() && list.get(0)!=null) : list;
Read r=list.get(0);
int numChroms=Data.numChroms=1;
SamHeader.PN="MicroAligner3";
Data.scaffoldNames=new byte[numChroms+1][][];
Data.scaffoldLocs=new int[numChroms+1][];
Data.scaffoldLengths=new int[numChroms+1][];
Data.chromScaffolds=new int[] {0, 1};
Data.scaffoldNames[1]=new byte[][] {Tools.trimToWhitespace(r.id.getBytes())};
Data.scaffoldLocs[1]=new int[] {0};
Data.scaffoldLengths[1]=new int[] {r.length()};
return r;
}
public void index() {indexRef(k, middleMask, ref, map);}
private static LongHashMap indexRef(int k, long midMask, byte[] ref, LongHashMap map) {
map.clear();
byte[] bases=ref;
assert(k<=32);
if(bases==null || bases.length<k){return map;}
final int bitsPerBase=2;
final int shift=bitsPerBase*k;
final int shift2=shift-bitsPerBase;
final long mask=(shift>63 ? -1L : ~((-1L)<<shift));
long kmer=0;
long rkmer=0;
int len=0;
for(int i=0; i<bases.length; i++){
final byte b=bases[i];
long x=AminoAcid.baseToNumber[b];
long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{
len++;
if(len>=k){
long key=Tools.max(kmer, rkmer)&midMask;
int value=(kmer>=rkmer ? i : i+MINUS_CODE);
// if(!map.containsKey(key)) {//Not needed, since old values are not replaced
map.put(key, value);
// }
}
}
}
return map;
}
/*--------------------------------------------------------------*/
/*---------------- Mapping ----------------*/
/*--------------------------------------------------------------*/
/**
* Returns first hit location.
* @param r Read to map.
* @return (offset<<1)|strand
*/
public long map(Read r) {
assert(!r.mapped() && r.match==null && r.samline==null);
r.chrom=-1;
if(r==null || r.length()<k || r.match!=null || r.samline!=null) {return 0;}
byte[] bases=r.bases;
if(bases==null || bases.length<k){return 0;}
final int bitsPerBase=2;
final int shift=bitsPerBase*k;
final int shift2=shift-bitsPerBase;
final long mask=(shift>63 ? -1L : ~((-1L)<<shift));
long kmer=0;
long rkmer=0;
int len=0;
int offset=-1;
int orientation=-1;
int ivalue=-1;
int vvalue=-1;
//TODO: This loop could be replaced by the kmer list which already exists.
for(int i=0; i<bases.length && orientation<0; i++){
final byte b=bases[i];
long x=AminoAcid.baseToNumber[b];
long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{
len++;
if(len>=k /*&& ((i&1)==0)*/){
long key=Tools.max(kmer, rkmer)&middleMask;
int value=map.get(key);
if(value>=0) {
ivalue=i;
vvalue=value;
if(value>=MINUS_CODE) {
value-=MINUS_CODE;
if(kmer>=rkmer) {
orientation=1;
offset=value-k2-(bases.length-i-1);
}else {
// assert(key==rkmer);
orientation=2;
offset=value-i;
}
}else {
if(kmer>=rkmer) {
orientation=0;
offset=value-i;
}else {
// assert(key==rkmer);
orientation=3;
offset=value-k2-(bases.length-i-1);
}
}
}
}
}
}
// assert(map.size()>0) : orientation+", "+offset+", "+map.size();
if(orientation<0) {return NO_HIT;}
r.chrom=1;
int strand=orientation&1;
r.setStrand(strand);
r.start=offset;
r.stop=offset+r.length()-1;
// r.setMapped(true);
return (offset<<1)|strand;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
public LongHashMap getMap() {return map;}
final int k;
final int k2;
final int midMaskLen;
final long middleMask;
final private String refname;
final byte[] ref;
final LongHashMap map;
//Indicates the position is on the minus strand
static final int MINUS_CODE=1000000000;
static final int NO_HIT=Integer.MIN_VALUE;
}
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