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package aligner;
import java.util.ArrayList;
import dna.Data;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import jgi.BBDuk;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadOutputStream;
import stream.Read;
import stream.ReadStreamByteWriter;
import stream.SamLine;
public class SideChannel3 {
/*--------------------------------------------------------------*/
/*---------------- Constructor ----------------*/
/*--------------------------------------------------------------*/
public SideChannel3(String ref_, String out_, String outu_, int k1_, float minid1,
int midMaskLen1, boolean overwrite_, boolean ordered_) {
this(ref_, out_, outu_, k1_, -1, minid1, 1, midMaskLen1, 0, overwrite_, ordered_);
}
public SideChannel3(String ref_, String out_, String outu_, int k1_, int k2_, float minid1, float minid2,
int midMaskLen1, int midMaskLen2, boolean overwrite_, boolean ordered_) {
Timer t=new Timer();
ref=fixRefPath(ref_);
out=out_;
outu=outu_;
k1=Tools.max(k1_, k2_);
k2=Tools.min(k1_, k2_);
minIdentity1=fixID(minid1);
minIdentity2=fixID(minid2);
overwrite=overwrite_;
ordered=ordered_;
assert(k1>0);
ffout=FileFormat.testOutput(out, FileFormat.SAM, null, true, overwrite, false, ordered);
ffoutu=FileFormat.testOutput(outu, FileFormat.FASTQ, null, true, overwrite, false, ordered);
samOut=((ffout!=null && ffout.samOrBam()) || ((ffoutu!=null && ffoutu.samOrBam())));
final Read r=MicroIndex3.loadRef(ref, samOut);
index1=new MicroIndex3(k1, midMaskLen1, r);
index2=(k2<1 ? null : new MicroIndex3(k2, midMaskLen2, r));
mapper1=new MicroAligner3(index1, minIdentity1, true);
mapper2=(k2<1 ? null : new MicroAligner3(index2, minIdentity2, true));
if(samOut) {ReadStreamByteWriter.USE_ATTACHED_SAMLINE=true;}
final int buff=(!ordered ? 12 : Tools.max(32, 2*Shared.threads()));
if(ffout!=null) {
cros=ConcurrentReadOutputStream.getStream(ffout, null, buff, null, false);
}else {cros=null;}
if(ffoutu!=null) {
crosu=ConcurrentReadOutputStream.getStream(ffoutu, null, buff, null, false);
}else {crosu=null;}
t.stop("Created side channel"+(out==null ? "" : (" for "+out))+": ");
}
/*--------------------------------------------------------------*/
/*---------------- Methods ----------------*/
/*--------------------------------------------------------------*/
public boolean map(Read r1, Read r2) {
return map(r1, r2, mapper1, mapper2);
}
public boolean map(Read r1, Read r2, MicroAligner3 mapper1, MicroAligner3 mapper2) {
float id1=mapper1.map(r1);
float id2=mapper1.map(r2);
if(id1+id2<=0) {return false;}//Common case
if(r2!=null) {
if(mapper2!=null) {
if(r1.mapped() && !r2.mapped()) {id2=mapper2.map(r2);}
else if(r2.mapped() && !r1.mapped()) {id1=mapper2.map(r1);}
}
boolean properPair=(r1.mapped() && r2.mapped() && r1.chrom==r2.chrom &&
r1.strand()!=r2.strand() && Tools.absdif(r1.start, r2.start)<=1000);
r1.setPaired(properPair);
r2.setPaired(properPair);
}
if(!r1.mapped()) {id1=0;}
if(r2==null || !r2.mapped()) {id2=0;}
long idsum=(long)((id1+id2)*10000);
if(idsum<=0) {return false;}
return true;
}
public String stats(long readsIn, long basesIn) {
long ro, bo, idsum, rm;
ro=readsOut; bo=basesOut; idsum=identitySum; rm=readsMapped;
String s=("Aligned reads: \t"+ro+" reads ("+BBDuk.toPercent(ro, readsIn)+") \t"+
+bo+" bases ("+BBDuk.toPercent(bo, basesIn)+") \tavgID="+Tools.format("%.4f", idsum/(100.0*rm)));
return s;
}
/*--------------------------------------------------------------*/
/*---------------- I/O ----------------*/
/*--------------------------------------------------------------*/
public void start() {
if(cros!=null) {cros.start();}
if(crosu!=null) {crosu.start();}
}
public boolean shutdown() {
errorState=ReadWrite.closeOutputStreams(cros, crosu)|errorState;
return errorState;
}
/** Everything in this list gets written */
public int writeToMapped(ArrayList<Read> reads, long num) {
if(reads==null || (reads.isEmpty() && !ordered) || (cros==null && !TRACK_STATS)) {
assert(!ordered || reads!=null);
return 0;
}
int rm=0, ro=0, bo=0;
double idsum=0;
for(Read r1 : reads) {
Read r2=r1.mate;
ro+=r1.pairCount();
bo+=r1.pairLength();
rm+=r1.pairMappedCount();
if(TRACK_STATS) {
if(r1.mapped()) {idsum+=r1.identity();}
if(r2!=null && r2.mapped()) {idsum+=r2.identity();}
}
}
if(TRACK_STATS && ro>0) {
synchronized(this) {
readsMapped+=rm;
readsOut+=ro;
basesOut+=bo;
identitySum+=(long)(idsum*10000);
}
}
if(cros==null) {return ro;}
final boolean makeSamLine=(samOut && ReadStreamByteWriter.USE_ATTACHED_SAMLINE);
if(makeSamLine) {
for(Read r1 : reads) {
Read r2=r1.mate;
r1.samline=(r1.samline!=null ? r1.samline : new SamLine(r1, 0));
if(r2!=null) {r2.samline=(r2.samline!=null ? r2.samline : new SamLine(r2, 1));}
}
}
cros.add(reads, num);
return ro;
}
/** Expects a list of mixed mapped and unmapped reads */
public int writeByStatus(ArrayList<Read> reads, long num) {
int ro=writeMappedOnly(reads, num);
writeUnmappedOnly(reads, num);
return ro;
}
/** Expects a list of mixed mapped and unmapped reads;
* only writes mapped reads (plus mates) */
private int writeMappedOnly(ArrayList<Read> reads, long num) {
if(reads==null || (reads.isEmpty() && !ordered)) {
assert(!ordered || reads!=null);
return 0;
}
int listSize=0;
int rm=0, ro=0, bo=0, rou=0, bou=0;
double idsum=0;
for(Read r1 : reads) {
boolean mapped=r1.eitherMapped();
if(mapped) {
Read r2=r1.mate;
listSize++;
ro+=r1.pairCount();
bo+=r1.pairLength();
rm+=r1.pairMappedCount();
if(TRACK_STATS) {
if(r1.mapped()) {idsum+=r1.identity();}
if(r2!=null && r2.mapped()) {idsum+=r2.identity();}
}
}else{
rou+=r1.pairCount();
bou+=r1.pairLength();
}
}
if(TRACK_STATS && ro>0) {
synchronized(this) {
readsMapped+=rm;
readsOut+=ro;
basesOut+=bo;
identitySum+=(long)(idsum*10000);
}
}
if(cros==null || (!ordered && listSize<1)) {return ro;}
ArrayList<Read> list=new ArrayList<Read>(Tools.max(1, listSize));
final boolean makeSamline=(samOut && ReadStreamByteWriter.USE_ATTACHED_SAMLINE);
for(Read r1 : reads) {
boolean mapped=r1.eitherMapped();
if(mapped && list!=null) {
Read r2=r1.mate;
if(makeSamline) {
r1.samline=(r1.samline!=null ? r1.samline : new SamLine(r1, 0));
if(r2!=null) {r2.samline=(r2.samline!=null ? r2.samline : new SamLine(r2, 1));}
}
list.add(r1);
}
}
cros.add(list, num);
return ro;
}
/** Expects a list of mixed mapped and unmapped reads;
* only writes unmapped reads with unmapped mates */
private int writeUnmappedOnly(ArrayList<Read> reads, long num) {
if(reads==null || crosu==null || (reads.isEmpty() && !ordered)) {
assert(!ordered || reads!=null);
return 0;
}
ArrayList<Read> list=new ArrayList<Read>(8);
int rou=0;
for(Read r1 : reads) {
if(!r1.eitherMapped()) {
rou+=r1.pairCount();
list.add(r1);
}
}
crosu.add(list, num);
return rou;
}
/*--------------------------------------------------------------*/
/*---------------- Static Methods ----------------*/
/*--------------------------------------------------------------*/
public static int[] parseK(String arg, String a, String b) {
int[] ret=new int[2];
String[] terms=b.split(",");
for(int i=0; i<terms.length; i++) {
ret[i]=Integer.parseInt(terms[i]);
}
return ret;
}
static float fixID(float id) {
if(id>1) {id=id/100;}
assert(id<=1);
return id;
}
static String fixRefPath(String refPath) {
if(refPath==null || Tools.isReadableFile(refPath)) {return refPath;}
if("phix".equalsIgnoreCase(refPath)){return Data.findPath("?phix2.fa.gz");}
return refPath;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
public boolean errorState=false;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
public final MicroIndex3 index1;
public final MicroIndex3 index2;
public final MicroAligner3 mapper1;
public final MicroAligner3 mapper2;
public final int k1;
public final int k2;
public final float minIdentity1;
public final float minIdentity2;
public final String ref;
public final String out;
public final String outu;
public final boolean samOut;
public final FileFormat ffout;
public final FileFormat ffoutu;
private final ConcurrentReadOutputStream cros;
private final ConcurrentReadOutputStream crosu;
public long readsMapped=0;
public long readsOut=0;
public long basesOut=0;
public long identitySum=0;//x100%; 0-10000 scale.
public final boolean overwrite;
public final boolean ordered;
/*--------------------------------------------------------------*/
/*---------------- Statics ----------------*/
/*--------------------------------------------------------------*/
public static boolean TRACK_STATS=true;
}
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