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package assemble;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.BitSet;
import java.util.concurrent.atomic.AtomicInteger;
import java.util.concurrent.atomic.AtomicLong;
import dna.AminoAcid;
import fileIO.ByteFile;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import kmer.AbstractKmerTable;
import kmer.AbstractKmerTableSet;
import kmer.HashArray1D;
import kmer.HashForest;
import kmer.KmerNode;
import kmer.KmerTableSet;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import sort.ContigLengthComparator;
import sort.ReadLengthComparator;
import stream.FastaReadInputStream;
import structures.ByteBuilder;
import structures.IntList;
import structures.LongList;
import tracker.ReadStats;
import ukmer.Kmer;
import ukmer.KmerTableSetU;
/**
* Assembles kmers into a concise representation.
* @author Brian Bushnell
* @date May 15, 2015
*
*/
public class KmerCompressor {
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer(), t2=new Timer();
t.start();
t2.start();
final KmerCompressor x=new KmerCompressor(args, true);
t2.stop();
outstream.println("Initialization Time: \t"+t2);
///And run it
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(outstream);
}
public static final int preparseK(String[] args){
int k=31;
for(int i=0; i<args.length; i++){
final String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(a.equals("k")){
k=Integer.parseInt(b);
}
}
return Kmer.getMult(k)*Kmer.getK(k);
}
/**
* Constructor.
* @param args Command line arguments
*/
public KmerCompressor(String[] args, boolean setDefaults){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
k=preparseK(args);
if(setDefaults){
/* Set global defaults */
ReadWrite.ZIPLEVEL=8;
ReadWrite.USE_UNPIGZ=true;
ReadWrite.USE_PIGZ=true;
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
AbstractKmerTableSet.defaultMinprob=0.5;
}
/* Initialize local variables with defaults */
Parser parser=new Parser();
ArrayList<String> in1=new ArrayList<String>();
ArrayList<String> in2=new ArrayList<String>();
int fuse_=0;
/* Parse arguments */
for(int i=0; i<args.length; i++){
final String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(parser.parseInterleaved(arg, a, b)){
//do nothing
}else if(parser.parseTrim(arg, a, b)){
//do nothing
}else if(a.equals("in") || a.equals("in1")){
in1.clear();
if(b!=null){
String[] s=b.split(",");
for(String ss : s){
in1.add(ss);
}
}
}else if(a.equals("in2")){
in2.clear();
if(b!=null){
String[] s=b.split(",");
for(String ss : s){
in2.add(ss);
}
}
}else if(a.equals("out") || a.equals("contigs")){
outContigs=b;
}else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
}else if(a.equals("fuse")){
if(b==null || Character.isLetter(b.charAt(0))){
fuse_=Parse.parseBoolean(b) ? 100000 : 0;
}else{
fuse_=Integer.parseInt(b);
}
}else if(a.equals("showstats") || a.equals("stats")){
showStats=Parse.parseBoolean(b);
}else if(a.equals("mincount") || a.equals("mincov") || a.equals("mindepth") || a.equals("min")){
minCount=Parse.parseIntKMG(b);
}else if(a.equals("maxcount") || a.equals("maxcov") || a.equals("maxdepth") || a.equals("max")){
maxCount=Parse.parseIntKMG(b);
}else if(a.equals("requiresamecount") || a.equals("rsc") || a.equals("rsd")){
REQUIRE_SAME_COUNT=Parse.parseBoolean(b);
}else if(a.equals("threads") || a.equals("t")){
Shared.setThreads(b);
}else if(a.equals("buildthreads") || a.equals("bthreads") || a.equals("bt")){
assert(b!=null) : "Bad parameter: "+arg;
if(b.equalsIgnoreCase("auto")){
BUILD_THREADS=Shared.threads();
}else{
BUILD_THREADS=Integer.parseInt(b);
}
}else if(a.equals("showspeed") || a.equals("ss")){
showSpeed=Parse.parseBoolean(b);
}else if(a.equals("verbose")){
// assert(false) : "Verbose flag is currently static final; must be recompiled to change.";
verbose=Parse.parseBoolean(b);
}else if(a.equals("verbose2")){
// assert(false) : "Verbose flag is currently static final; must be recompiled to change.";
verbose2=Parse.parseBoolean(b);
}else if(a.equals("ilb") || a.equals("ignoreleftbranches") || a.equals("ignoreleftjunctions") || a.equals("ibb") || a.equals("ignorebackbranches")){
extendThroughLeftJunctions=Parse.parseBoolean(b);
}else if(a.equals("rcomp")){
doRcomp=Parse.parseBoolean(b);
}
else if(KmerTableSetU.isValidArgument(a)){
//Do nothing
}else{
throw new RuntimeException("Unknown parameter "+args[i]);
}
}
fuse=fuse_;
LOAD_THREADS=Shared.threads();
{//Process parser fields
Parser.processQuality();
}
/* Adjust I/O settings and filenames */
assert(FastaReadInputStream.settingsOK());
nextTable=new AtomicInteger[1];
nextVictims=new AtomicInteger[1];
for(int i=0; i<1; i++){
nextTable[i]=new AtomicInteger(0);
nextVictims[i]=new AtomicInteger(0);
}
if(!Tools.testOutputFiles(overwrite, append, false, outContigs)){
throw new RuntimeException("\nCan't write to some output files; overwrite="+overwrite+"\n");
}
assert(LOAD_THREADS>0);
outstream.println("Using "+LOAD_THREADS+" threads.");
final int bytesPerKmer;
{
int mult=12+k; //worst case for no assembly;
if(true){mult+=4;}
bytesPerKmer=mult;
}
tables=new KmerTableSet(args, bytesPerKmer);
k2=tables.k2;
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
public final void process(Timer t){
/* Count kmers */
process2();
/* Stop timer and calculate speed statistics */
t.stop();
if(showSpeed){
outstream.println("\nTotal Time: \t"+t);
}
if(showStats && outContigs!=null && FileFormat.isFastaExt(ReadWrite.rawExtension(outContigs)) && !FileFormat.isStdio(outContigs)){
outstream.println();
jgi.AssemblyStats2.main(new String[] {"in="+outContigs});
}
/* Throw an exception if errors were detected */
if(errorState){
throw new RuntimeException(getClass().getSimpleName()+" terminated in an error state; the output may be corrupt.");
}
}
public long loadKmers(Timer t){
tables.process(t);
return tables.kmersLoaded;
}
public final void process2(){
/* Start phase timer */
Timer t=new Timer();
/* Fill tables with kmers */
outstream.println("\nLoading kmers.\n");
loadKmers(t);
t.stop();
// outstream.println("Input: \t"+tables.readsIn+" reads \t\t"+tables.basesIn+" bases.");
// outstream.println("Unique Kmers: \t"+tables.kmersLoaded);
// outstream.println("Load Time: \t"+t);
t.start();
{
/* Build contigs */
outstream.println("\nBuilding contigs.\n");
buildContigs();
if(DISPLAY_PROGRESS){
outstream.println("\nAfter building contigs:");
Shared.printMemory();
outstream.println();
}
t.stop();
if(readsIn>0){outstream.println("Input: \t"+readsIn+" reads \t\t"+basesIn+" bases.");}
outstream.println("Bases generated: \t"+basesBuilt);
outstream.println("Contigs generated: \t"+contigsBuilt);
outstream.println("Longest contig: \t"+longestContig);
outstream.println("Contig-building time: \t"+t);
}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
/**
* Build contigs.
*/
private final void buildContigs(){
allContigs=new ArrayList<Contig>();
tables.initializeOwnership();
/* Create ProcessThreads */
ArrayList<AbstractBuildThread> alpt=new ArrayList<AbstractBuildThread>(BUILD_THREADS);
for(int i=0; i<BUILD_THREADS; i++){alpt.add(makeBuildThread(i));}
for(AbstractBuildThread pt : alpt){pt.start();}
/* Wait for threads to die, and gather statistics */
for(AbstractBuildThread pt : alpt){
while(pt.getState()!=Thread.State.TERMINATED){
try {
pt.join();
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
for(Contig contig : pt.contigs){
allContigs.add(contig);
contigsBuilt++;
basesBuilt+=contig.length();
longestContig=Tools.max(longestContig, contig.length());
}
readsIn+=pt.readsInT;
basesIn+=pt.basesInT;
lowqReads+=pt.lowqReadsT;
lowqBases+=pt.lowqBasesT;
}
if(outContigs!=null){
FileFormat ff=FileFormat.testOutput(outContigs, FileFormat.FA, 0, 0, true, overwrite, append, false);
// ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(ff, null, null, null, 4, null, false);
// ros.start();
ByteStreamWriter bsw=new ByteStreamWriter(ff);
bsw.start();
if(allContigs!=null){
// Shared.sort(allContigs, ReadComparatorID.comparator);
ReadLengthComparator.comparator.setAscending(false);
Shared.sort(allContigs, ContigLengthComparator.comparator);
fuse(allContigs, fuse);
for(int i=0; i<allContigs.size(); i++){
Contig r=allContigs.get(i);
bsw.println(r);
}
}
errorState|=bsw.poisonAndWait();
}
}
private static void fuse(ArrayList<Contig> contigs, int fuse){
if(fuse<2){return;}
ArrayList<Contig> temp=new ArrayList<Contig>();
ByteBuilder bb=new ByteBuilder();
int num=0;
for(int i=0; i<contigs.size(); i++){
Contig r=contigs.set(i, null);
if(bb.length()>0){bb.append('N');}
bb.append(r.bases);
if(bb.length()>=fuse){
Contig fused=new Contig(bb.toBytes(), num);
num++;
temp.add(fused);
bb.clear();
}
}
if(bb.length()>0){
Contig fused=new Contig(bb.toBytes(), num);
num++;
temp.add(fused);
bb.clear();
}
contigs.clear();
contigs.addAll(temp);
temp=null;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- BuildThread ----------------*/
/*--------------------------------------------------------------*/
BuildThread makeBuildThread(int id){
return new BuildThread(id);
}
/**
* Builds contigs.
*/
private class BuildThread extends AbstractBuildThread{
public BuildThread(int id_){
super(id_, Tadpole.contigMode, null);
}
@Override
public void run(){
//Build from kmers
//Final pass
while(processNextTable(nextTable[0])){}
while(processNextVictims(nextVictims[0])){}
}
private boolean processNextTable(AtomicInteger aint){
final int tnum=aint.getAndAdd(1);
if(tnum>=tables.ways){return false;}
final HashArray1D table=tables.getTable(tnum);
if(verbose && id==0){System.err.println("Processing table "+tnum+", size "+table.size());}
final int max=table.arrayLength();
for(int cell=0; cell<max; cell++){
int x=processCell(table, cell);
}
return true;
}
private boolean processNextVictims(AtomicInteger aint){
final int tnum=aint.getAndAdd(1);
if(tnum>=tables.ways){return false;}
final HashArray1D table=tables.getTable(tnum);
final HashForest forest=table.victims();
if(verbose && id==0){System.err.println("Processing forest "+tnum+", size "+forest.size());}
final int max=forest.arrayLength();
for(int cell=0; cell<max; cell++){
KmerNode kn=forest.getNode(cell);
int x=traverseKmerNode(kn);
}
return true;
}
private int processCell(HashArray1D table, int cell){
int count=table.readCellValue(cell);
if(count<minCount || count>maxCount){return 0;}
long key=table.getKmer(cell);
if(verbose){outstream.println("id="+id+" processing cell "+cell+"; \tkmer="+key+"\t"+toText(key));}
int owner=table.getCellOwner(cell);
if(verbose){outstream.println("Owner is initially "+owner);}
if(owner>-1){return 0;}
owner=table.setOwner(key, id, cell);
if(verbose){outstream.println("Owner is now "+owner);}
if(owner!=id){return 0;}
return processKmer(key);
}
private int traverseKmerNode(KmerNode kn){
int sum=0;
if(kn!=null){
sum+=processKmerNode(kn);
if(kn.left()!=null){
sum+=traverseKmerNode(kn.left());
}
if(kn.right()!=null){
sum+=traverseKmerNode(kn.right());
}
}
return sum;
}
private int processKmerNode(KmerNode kn){
final long key=kn.pivot();
final int count=kn.getValue(key);
if(count<minCount || count>maxCount){return 0;}
if(verbose){outstream.println("id="+id+" processing KmerNode; \tkmer="+key+"\t"+toText(key));}
int owner=kn.getOwner(key);
if(verbose){outstream.println("Owner is initially "+owner);}
if(owner>-1){return 0;}
owner=kn.setOwner(key, id);
if(verbose){outstream.println("Owner is now "+owner);}
if(owner!=id){return 0;}
return processKmer(key);
}
/** Returns length of new contig */
private int processKmer(long key){
byte[] bases=makeContig(key, builderT, true);
if(bases!=null){
final long num=contigNum.incrementAndGet();
final String id;
if(REQUIRE_SAME_COUNT){
id="n"+num+",c="+tables.getCount(key);
}else{
id=Long.toString(num);
}
Contig r=new Contig(bases, id, (int)num);
contigs.add(r);
if(verbose){System.err.println("Added "+bases.length);}
return bases.length;
}else{
if(verbose){System.err.println("Created null contig.");}
}
return 0;
}
/** From kmers */
private byte[] makeContig(final long key, final ByteBuilder bb, boolean alreadyClaimed){
builderT.setLength(0);
builderT.appendKmer(key, k);
if(verbose){outstream.println("Filled builder: "+builderT);}
final int initialLength=bb.length();
assert(initialLength==k);
if(initialLength<k){return null;}
// System.err.print("A");
{
boolean success=(alreadyClaimed || claim(key, id));
if(verbose){System.err.println("Thread "+id+" checking owner after setting: "+findOwner(bb, id));}
if(!success){
assert(bb.length()==k);
// release(bb, id); //no need to release
return null;
}
}
// System.err.print("B");
if(verbose /*|| true*/){System.err.println("Thread "+id+" building contig; initial length "+bb.length());}
if(verbose){System.err.println("Extending to right.");}
{
final int status=extendToRight(bb, rightCounts, id);
if(status==DEAD_END){
//do nothing
}else if(status==TOO_LONG){
//do nothing
}else if(status==BAD_SEED){
if(bb.length()<=k){
release(key, id);
return null;
}
}else{
throw new RuntimeException("Bad return value: "+status);
}
}
// System.err.print("C");
bb.reverseComplementInPlace();
if(verbose /*|| true*/){System.err.println("Extending rcomp to right; current length "+bb.length());}
{
final int status;
if(doRcomp){
status=extendToRight(bb, rightCounts, id);
}else{
status=extendToRight_RcompOnly(bb, rightCounts, id);
}
if(status==DEAD_END){
//do nothing
}else if(status==TOO_LONG){
//do nothing
}else if(status==BAD_SEED){
if(bb.length()<=k){
release(key, id);
return null;
}
}else{
throw new RuntimeException("Bad return value: "+status);
}
}
// System.err.print("D");
if(verbose /*|| true*/){System.err.println("A: Final length for thread "+id+": "+bb.length());}
//TODO: Success only if this thread actually owns some kmer in the contig. And trim unowned terminal kmers.
if(bb.length()>=k){
bb.reverseComplementInPlace();
return bb.toBytes();
}
if(verbose /*|| true*/){System.err.println("A: Contig was too short for "+id+": "+bb.length());}
// assert(false) : bb.length()+", "+initialLength+", "+minExtension+", "+minContigLen;
// System.err.print("F");
return null;
}
}
/*--------------------------------------------------------------*/
/*---------------- Extension Methods ----------------*/
/*--------------------------------------------------------------*/
/**
* Extend these bases into a contig.
* Stops at both left and right junctions.
* Claims ownership.
*/
public int extendToRight(final ByteBuilder bb, final int[] rightCounts, final int id){
if(bb.length()<k){return BAD_SEED;}
final int shift=2*k;
final int shift2=shift-2;
final long mask=(shift>63 ? -1L : ~((-1L)<<shift));
long kmer=0;
long rkmer=0;
int len=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
{
final int bblen=bb.length();
final byte[] bases=bb.array;
for(int i=bblen-k; i<bblen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{len++;}
if(verbose){outstream.println("A: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
}
if(len<k){
if(verbose){outstream.println("Returning BAD_SEED 1");}
return BAD_SEED;
}
else{assert(len==k);}
/* Now the trailing kmer has been initialized. */
long key=toValue(kmer, rkmer);
HashArray1D table=tables.getTableForKey(key);
int count=table.getValue(key);
if(count<minCount || count>maxCount){
if(verbose){
outstream.println("Returning because count was too low: "+count);
outstream.println("Returning BAD_SEED 2");
}
return BAD_SEED;
}
int owner=table.getOwner(key);
if(verbose){outstream.println("Owner: "+owner);}
if(owner>-1 && owner!=id){
if(verbose){outstream.println("Returning BAD_SEED 3");}
return BAD_SEED;
}
owner=table.setOwner(key, id);
if(verbose){outstream.println("A. Owner is now "+id+" for key "+key);}
if(owner!=id){
if(verbose){
outstream.println("Returning early because owner was "+owner+" for thread "+id+".");
outstream.println("Returning BAD_SEED 4");
}
return BAD_SEED;
}
final int maxLen=Tools.max(100000, bb.length()+90000);
while(bb.length()<maxLen){
fillRightCounts(kmer, rkmer, rightCounts, mask, shift2);
int selected=-1;
for(int i=0; i<4; i++){
final int count2=rightCounts[i];
if(count2>=minCount && count2<=maxCount && (!REQUIRE_SAME_COUNT || count2==count)){
final long y=i;
final long y2=AminoAcid.numberToComplement[i];
final long kmer2=((kmer<<2)|(long)y)&mask;
final long rkmer2=(rkmer>>>2)|(y2<<shift2);
final long key2=toValue(kmer2, rkmer2);
HashArray1D table2=tables.getTableForKey(key2);
if(table2.getOwner(key2)<0){
if(table2.setOwner(key2, id)==id){
selected=i;
kmer=kmer2;
rkmer=rkmer2;
key=key2;
count=count2;
final byte b=AminoAcid.numberToBase[selected];
bb.append(b);
break;
}
}
}
}
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+Arrays.toString(rightCounts));
}
if(selected<0){
if(verbose){outstream.println("Returning DEAD_END");}
return DEAD_END;
}//TODO: Explore on failure
}
if(verbose){
outstream.println("Current contig length: "+bb.length()+"\nReturning TOO_LONG");
}
// assert(owner!=id) : owner+"!="+id+"; maxLen="+maxLen+"; len="+bb.length();
return TOO_LONG;
}
/**
* Extend these bases into a contig.
* Stops at both left and right junctions.
* Claims ownership.
*/
public int extendToRight_RcompOnly(final ByteBuilder bb, final int[] rightCounts, final int id){
if(bb.length()<k){return BAD_SEED;}
final int shift=2*k;
final int shift2=shift-2;
final long mask=(shift>63 ? -1L : ~((-1L)<<shift));
long kmer=0;
long rkmer=0;
int len=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
{
final int bblen=bb.length();
final byte[] bases=bb.array;
for(int i=bblen-k; i<bblen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{len++;}
if(verbose){outstream.println("A: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
}
if(len<k){
if(verbose){outstream.println("Returning BAD_SEED 1");}
return BAD_SEED;
}
else{assert(len==k);}
/* Now the trailing kmer has been initialized. */
long key=rkmer;
HashArray1D table=tables.getTableForKey(key);
int count=table.getValue(key);
if(count<minCount || count>maxCount){
if(verbose){
outstream.println("Returning because count was too low: "+count);
outstream.println("Returning BAD_SEED 2");
}
return BAD_SEED;
}
int owner=table.getOwner(key);
if(verbose){outstream.println("Owner: "+owner);}
if(owner>-1 && owner!=id){
if(verbose){outstream.println("Returning BAD_SEED 3");}
return BAD_SEED;
}
owner=table.setOwner(key, id);
if(verbose){outstream.println("A. Owner is now "+id+" for key "+key);}
if(owner!=id){
if(verbose){
outstream.println("Returning early because owner was "+owner+" for thread "+id+".");
outstream.println("Returning BAD_SEED 4");
}
return BAD_SEED;
}
final int maxLen=Tools.max(100000, bb.length()+90000);
while(bb.length()<maxLen){
fillRightCountsRcompOnly(kmer, rkmer, rightCounts, mask, shift2);
int selected=-1;
for(int i=0; i<4; i++){
final int count2=rightCounts[i];
if(count2>=minCount && count2<=maxCount && (!REQUIRE_SAME_COUNT || count2==count)){
final long y=i;
final long y2=AminoAcid.numberToComplement[i];
final long kmer2=((kmer<<2)|(long)y)&mask;
final long rkmer2=(rkmer>>>2)|(y2<<shift2);
final long key2=rkmer2;
HashArray1D table2=tables.getTableForKey(key2);
if(table2.getOwner(key2)<0){
if(table2.setOwner(key2, id)==id){
selected=i;
kmer=kmer2;
rkmer=rkmer2;
key=key2;
count=count2;
final byte b=AminoAcid.numberToBase[selected];
bb.append(b);
break;
}
}
}
}
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+Arrays.toString(rightCounts));
}
if(selected<0){
if(verbose){outstream.println("Returning DEAD_END");}
return DEAD_END;
}//TODO: Explore on failure
}
if(verbose){
outstream.println("Current contig length: "+bb.length()+"\nReturning TOO_LONG");
}
// assert(owner!=id) : owner+"!="+id+"; maxLen="+maxLen+"; len="+bb.length();
return TOO_LONG;
}
/*--------------------------------------------------------------*/
/*---------------- Helper Methods ----------------*/
/*--------------------------------------------------------------*/
/** Currently unused */
protected final static Kmer getKmer(byte[] bases, int loc, Kmer kmer){
kmer.clear();
for(int i=loc, lim=loc+kmer.k; i<lim; i++){
byte b=bases[i];
int x=AminoAcid.baseToNumber[b];
if(x<0){return null;}
kmer.addRightNumeric(x);
}
assert(kmer.len==kmer.k);
return kmer;
}
/*--------------------------------------------------------------*/
/*---------------- Recall Methods ----------------*/
/*--------------------------------------------------------------*/
private final long rcomp(long kmer){return AminoAcid.reverseComplementBinaryFast(kmer, k);}
private final long toValue(long kmer, long rkmer){return tables.toValue(kmer, rkmer);}
public final int getCount(long kmer, long rkmer){return tables.getCount(kmer, rkmer);}
final boolean claim(long kmer, int id){return claim(kmer, rcomp(kmer), id);}
private final boolean claim(long kmer, long rkmer, int id){return tables.claim(kmer, rkmer, id);}
final int findOwner(ByteBuilder bb, int id){return tables.findOwner(bb, id);}
final void release(long key, int id){tables.release(key, id);}
private final int fillRightCounts(long kmer, long rkmer, int[] counts, long mask, int shift2){return tables.fillRightCounts(kmer, rkmer, counts, mask, shift2);}
private final int fillRightCountsRcompOnly(long kmer, long rkmer, int[] counts, long mask, int shift2){return tables.fillRightCountsRcompOnly(kmer, rkmer, counts, mask, shift2);}
final StringBuilder toText(long kmer){return AbstractKmerTable.toText(kmer, k);}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
public final KmerTableSet tables;
/** Normal kmer length */
final int k;
/** k-1; used in some expressions */
private final int k2;
private ArrayList<Contig> allContigs;
private long contigsBuilt=0;
private long basesBuilt=0;
private long longestContig=0;
protected boolean extendThroughLeftJunctions=true;
int minCount=1;
int maxCount=Integer.MAX_VALUE;
/** Only extend to kmers with the same count as this kmer */
boolean REQUIRE_SAME_COUNT=false;
public boolean showStats=true;
/** Has this class encountered errors while processing? */
public boolean errorState=false;
/** Contig output file */
private String outContigs=null;
long readsIn=0;
long basesIn=0;
long readsOut=0;
long basesOut=0;
long lowqReads=0;
long lowqBases=0;
/*--------------------------------------------------------------*/
/*---------------- ThreadLocal Temps ----------------*/
/*--------------------------------------------------------------*/
protected final void initializeThreadLocals(){
if(localRightCounts.get()!=null){return;}
localRightCounts.set(new int[4]);
localLongList.set(new LongList());
localIntList.set(new IntList());
localByteBuilder.set(new ByteBuilder());
localBitSet.set(new BitSet(300));
localKmer.set(new Kmer(k));
}
protected ThreadLocal<int[]> localRightCounts=new ThreadLocal<int[]>();
protected ThreadLocal<LongList> localLongList=new ThreadLocal<LongList>();
protected ThreadLocal<IntList> localIntList=new ThreadLocal<IntList>();
protected ThreadLocal<ByteBuilder> localByteBuilder=new ThreadLocal<ByteBuilder>();
protected ThreadLocal<BitSet> localBitSet=new ThreadLocal<BitSet>();
protected ThreadLocal<Kmer> localKmer=new ThreadLocal<Kmer>();
/*--------------------------------------------------------------*/
/*---------------- Final Primitives ----------------*/
/*--------------------------------------------------------------*/
/** For numbering contigs */
final AtomicLong contigNum=new AtomicLong(0);
/** For controlling access to tables for contig-building */
final AtomicInteger nextTable[];
/** For controlling access to victim buffers for contig-building */
final AtomicInteger nextVictims[];
final int fuse;
/*--------------------------------------------------------------*/
/*---------------- Static Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print messages to this stream */
protected static PrintStream outstream=System.err;
/** Permission to overwrite existing files */
public static boolean overwrite=true;
/** Permission to append to existing files */
public static boolean append=false;
/** Print speed statistics upon completion */
public static boolean showSpeed=true;
/** Display progress messages such as memory usage */
public static boolean DISPLAY_PROGRESS=true;
/** Verbose messages */
public static boolean verbose=false;
/** Debugging verbose messages */
public static boolean verbose2=false;
/** Reverse-complement */
public static boolean doRcomp=true;
/** Number of load threads */
public static int LOAD_THREADS=Shared.threads();
/** Number of build threads */
public static int BUILD_THREADS=1;
/** Explore codes */
public static final int KEEP_GOING=0, DEAD_END=1, TOO_SHORT=2, TOO_LONG=3, TOO_DEEP=4;
/** Extend codes */
public static final int BAD_SEED=12;
public static final int STATUS_UNEXPLORED=0, STATUS_EXPLORED=1, STATUS_REMOVE=2, STATUS_KEEP=3;
}
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