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package assemble;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.BitSet;
import java.util.concurrent.atomic.AtomicInteger;
import dna.AminoAcid;
import jgi.BBMerge;
import kmer.AbstractKmerTable;
import kmer.HashArray1D;
import kmer.HashForest;
import kmer.KmerNode;
import kmer.KmerTableSet;
import shared.KillSwitch;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.IntList;
import structures.ListNum;
import structures.LongList;
import ukmer.Kmer;
/**
* Short-kmer assembler based on KmerCountExact.
* @author Brian Bushnell
* @date May 15, 2015
*
*/
public class Tadpole1 extends Tadpole {
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer(), t2=new Timer();
t.start();
t2.start();
//Create a new CountKmersExact instance
Tadpole1 wog=new Tadpole1(args, true);
t2.stop();
outstream.println("Initialization Time: \t"+t2);
///And run it
wog.process(t);
}
/**
* Constructor.
* @param args Command line arguments
*/
public Tadpole1(String[] args, boolean setDefaults){
super(args, setDefaults);
final int bytesPerKmer;
{
int mult=12;
if(useOwnership){mult+=4;}
if(processingMode==correctMode || processingMode==discardMode){}
else if(processingMode==contigMode || processingMode==extendMode){mult+=1;}
bytesPerKmer=mult;
}
tables=new KmerTableSet(args, bytesPerKmer);
k=tables.k;
k2=tables.k2;
shift=2*k;
shift2=shift-2;
mask=(shift>63 ? -1L : ~((-1L)<<shift));
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
void initializeOwnership(){
tables.initializeOwnership();
}
@Override
long shave(boolean shave, boolean rinse){
long sum=0;
for(int i=0; i<maxShaveDepth; i++){
int a=1, b=maxShaveDepth, c=i+1;
// if(i>3){Shaver.verbose2=true;}
outstream.println("\nShave("+a+", "+b+", "+c+")");
final Shaver shaver=Shaver.makeShaver(tables, THREADS, a, b, c, minCountExtend, branchMult2, Tools.max(minContigLen, shaveDiscardLen), shaveExploreDist, shave, rinse);
long removed=shaver.shave(a, b);
sum+=removed;
if(removed<100 || i>2){break;}
}
outstream.println();
return sum;
}
@Override
public long loadKmers(Timer t){
tables.process(t);
return tables.kmersLoaded;
}
/*--------------------------------------------------------------*/
/*---------------- Recall Methods ----------------*/
/*--------------------------------------------------------------*/
final long rcomp(long kmer){return AminoAcid.reverseComplementBinaryFast(kmer, k);}
final long toValue(long kmer, long rkmer){return tables.toValue(kmer, rkmer);}
public final int getCount(long kmer, long rkmer){return tables.getCount(kmer, rkmer);}
final boolean claim(long kmer, int id){return claim(kmer, rcomp(kmer), id);}
final boolean claim(long kmer, long rkmer, int id){return tables.claim(kmer, rkmer, id);}
final boolean doubleClaim(ByteBuilder bb, int id/*, long rid*/){return tables.doubleClaim(bb, id/*, rid*/);}
final boolean claim(ByteBuilder bb, int id, /*long rid, */boolean earlyExit){return tables.claim(bb, id/*, rid*/, earlyExit);}
final boolean claim(byte[] array, int len, int id, /*long rid, */boolean earlyExit){return tables.claim(array, len, id/*, rid*/, earlyExit);}
final int findOwner(long kmer){return tables.findOwner(kmer);}
final int findOwner(ByteBuilder bb, int id){return tables.findOwner(bb, id);}
final int findOwner(byte[] array, int len, int id){return tables.findOwner(array, len, id);}
final void release(long key, int id){tables.release(key, id);}
final void release(ByteBuilder bb, int id){tables.release(bb, id);}
final void release(byte[] array, int len, int id){tables.release(array, len, id);}
final int fillRightCounts(long kmer, long rkmer, int[] counts){return tables.fillRightCounts(kmer, rkmer, counts, mask, shift2);}
final int fillLeftCounts(long kmer, long rkmer, int[] counts){return tables.fillLeftCounts(kmer, rkmer, counts, mask, shift2);}
final StringBuilder toText(long kmer){return AbstractKmerTable.toText(kmer, k);}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- BuildThread ----------------*/
/*--------------------------------------------------------------*/
@Override
BuildThread makeBuildThread(int id, int mode, ConcurrentReadInputStream[] crisa){
return new BuildThread(id, mode, crisa);
}
/**
* Builds contigs.
*/
private class BuildThread extends AbstractBuildThread{
public BuildThread(int id_, int mode_, ConcurrentReadInputStream[] crisa_){
super(id_, mode_, crisa_);
}
@Override
public void run(){
if(crisa==null || crisa.length==0){
//Build from kmers
if(id==0){outstream.print("Seeding with min count = ");}
String comma="";
for(int i=contigPasses-1; i>0; i--){
minCountSeedCurrent=(int)Tools.min(Integer.MAX_VALUE, Tools.max(minCountSeed+i, (long)Math.floor((minCountSeed)*Math.pow(contigPassMult, i)*0.92-0.25) ));
if(id==0){
outstream.print(comma+minCountSeedCurrent);
comma=", ";
}
while(processNextTable(nextTable[i])){}
while(processNextVictims(nextVictims[i])){}
}
//Final pass
minCountSeedCurrent=minCountSeed;
if(id==0){outstream.println(comma+minCountSeedCurrent);}
while(processNextTable(nextTable[0])){}
while(processNextVictims(nextVictims[0])){}
}else{
//Extend reads
for(ConcurrentReadInputStream cris : crisa){
synchronized(crisa){
if(!cris.started()){
cris.start();
}
}
run(cris);
}
}
}
private boolean processNextTable(AtomicInteger aint){
final int tnum=aint.getAndAdd(1);
if(tnum>=tables.ways){return false;}
final HashArray1D table=tables.getTable(tnum);
if(verbose2 && id==0){outstream.println("Processing table "+tnum+", size "+table.size());}
final int max=table.arrayLength();
for(int cell=0; cell<max; cell++){
int x=processCell(table, cell);
}
return true;
}
private boolean processNextVictims(AtomicInteger aint){
final int tnum=aint.getAndAdd(1);
if(tnum>=tables.ways){return false;}
final HashArray1D table=tables.getTable(tnum);
final HashForest forest=table.victims();
if(verbose2 && id==0){outstream.println("Processing forest "+tnum+", size "+forest.size());}
final int max=forest.arrayLength();
for(int cell=0; cell<max; cell++){
KmerNode kn=forest.getNode(cell);
int x=traverseKmerNode(kn);
}
return true;
}
private int processCell(HashArray1D table, int cell){
int count=table.readCellValue(cell);
if(count<minCountSeedCurrent){return 0;}
long key=table.getKmer(cell);
if(verbose){outstream.println("id="+id+" processing cell "+cell+"; \tkmer="+key+"\t"+toText(key));}
if(useOwnership){
int owner=table.getCellOwner(cell);
if(verbose){outstream.println("Owner is initially "+owner);}
if(owner>-1){return 0;}
owner=table.setOwner(key, id, cell);
if(verbose){outstream.println("Owner is now "+owner);}
if(owner!=id){return 0;}
}
return processKmer(key);
}
private int traverseKmerNode(KmerNode kn){
int sum=0;
if(kn!=null){
sum+=processKmerNode(kn);
if(kn.left()!=null){
sum+=traverseKmerNode(kn.left());
}
if(kn.right()!=null){
sum+=traverseKmerNode(kn.right());
}
}
return sum;
}
private int processKmerNode(KmerNode kn){
final long key=kn.pivot();
final int count=kn.getValue(key);
if(count<minCountSeedCurrent){return 0;}
if(verbose){outstream.println("id="+id+" processing KmerNode; \tkmer="+key+"\t"+toText(key));}
if(useOwnership){
int owner=kn.getOwner(key);
if(verbose){outstream.println("Owner is initially "+owner);}
if(owner>-1){return 0;}
owner=kn.setOwner(key, id);
if(verbose){outstream.println("Owner is now "+owner);}
if(owner!=id){return 0;}
}
return processKmer(key);
}
private int processKmer(long key){
Contig contig=makeContig(key, builderT, true);
if(contig!=null){
float coverage=tables.calcCoverage(contig);
if(coverage<minCoverage || coverage>maxCoverage){return 0;}
if(verbose){outstream.println("Added "+contig.length());}
contig.id=(int)contigNum.incrementAndGet();
contigs.add(contig);
return contig.length();
}else{
if(verbose){outstream.println("Created null contig.");}
}
return 0;
}
private void run(ConcurrentReadInputStream cris){
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
//While there are more reads lists...
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
//For each read (or pair) in the list...
for(int i=0; i<reads.size(); i++){
final Read r1=reads.get(i);
final Read r2=r1.mate;
processReadPair(r1, r2);
}
//Fetch a new read list
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
cris.returnList(ln);
}
private void processReadPair(Read r1, Read r2){
if(verbose){outstream.println("Considering read "+r1.id+" "+new String(r1.bases));}
readsInT++;
basesInT+=r1.length();
if(r2!=null){
readsInT++;
basesInT+=r2.length();
}
if(mode==insertMode){
int x=BBMerge.findOverlapStrict(r1, r2, false);
if(x<1){
x=findInsertSize(r1, r2, rightCounts);
}
insertSizes.increment(Tools.max(x, 0));
return;
}
if(ecco && r1!=null && r2!=null && !r1.discarded() && !r2.discarded()){BBMerge.findOverlapStrict(r1, r2, true);}
if(r1!=null){
if(r1.discarded()){
lowqBasesT+=r1.length();
lowqReadsT++;
}else{
byte[] bases=makeContig(r1.bases, builderT, r1.numericID);
if(bases!=null){
if(verbose){outstream.println("Added "+bases.length);}
final long num=contigNum.incrementAndGet();
Contig temp=new Contig(bases, "contig_"+num+"_length_"+bases.length, (int)num);
contigs.add(temp);
}
}
}
if(r2!=null){
if(r2.discarded()){
lowqBasesT+=r2.length();
lowqReadsT++;
}else{
byte[] bases=makeContig(r2.bases, builderT, r2.numericID);
if(bases!=null){
if(verbose){outstream.println("Added "+bases.length);}
final long num=contigNum.incrementAndGet();
Contig temp=new Contig(bases, "contig_"+num+"_length_"+bases.length, (int)num);
contigs.add(temp);
}
}
}
}
/** From kmers */
private Contig makeContig(final long key, final ByteBuilder bb, boolean alreadyClaimed){
builderT.setLength(0);
builderT.appendKmer(key, k);
if(verbose){outstream.println("Filled builder: "+builderT);}
final int initialLength=bb.length();
assert(initialLength==k);
if(initialLength<k){return null;}
// outstream.print("A");
boolean success=(alreadyClaimed || !useOwnership ? true : claim(key, id));
if(verbose){outstream.println("Thread "+id+" checking owner after setting: "+findOwner(bb, id));}
if(!success){
assert(bb.length()==k);
// release(bb, id); //no need to release
return null;
}
// outstream.print("B");
if(verbose /*|| true*/){outstream.println("Thread "+id+" building contig; initial length "+bb.length());}
if(verbose){outstream.println("Extending to right.");}
final int rightStatus, leftStatus;
float leftRatio=0, rightRatio=0;
{
final int status=extendToRight(bb, leftCounts, rightCounts, id);
if(status==DEAD_END){
//do nothing
}else if(status==LOOP){//TODO
//special case - handle specially, for a loop with no obvious junction, e.g. long tandem repeat.
//Perhaps, the last kmer should be reclassified as a junction and removed.
}else if(status==BAD_SEED){
assert(bb.length()==k);
release(key, id);
// outstream.print("B1");
return null;
}else{
if(bb.length()==k){
if(status==BAD_OWNER){
if(IGNORE_BAD_OWNER){
//do nothing
}else{
release(key, id);
// outstream.print("B2");
return null;
}
}else if(isBranchCode(status)){
release(key, id);
// outstream.print("B3");
return null;
}else{
throw new RuntimeException("Bad return value: "+status);
}
}else{
if(status==BAD_OWNER){
if(IGNORE_BAD_OWNER){
bb.length--;
}else{
release(bb, id);
// outstream.print("B4");
return null;
}
}else if(status==F_BRANCH || status==D_BRANCH){
rightRatio=calcRatio(rightCounts);
}else if(status==B_BRANCH){
rightRatio=calcRatio(leftCounts);
}else{
throw new RuntimeException("Bad return value: "+status);
}
}
}
rightStatus=status;
}
// outstream.print("C");
bb.reverseComplementInPlace();
if(verbose /*|| true*/){outstream.println("Extending rcomp to right; current length "+bb.length());}
{
final int status=extendToRight(bb, leftCounts, rightCounts, id);
if(status==DEAD_END){
//do nothing
}else if(status==LOOP){//TODO
//special case - handle specially, for a loop with no obvious junction, e.g. long tandem repeat.
//Perhaps, the last kmer should be reclassified as a junction and removed.
}else if(status==BAD_SEED){
assert(false) : bb;//This should never happen.
assert(bb.length()==k);
release(key, id);
return null;
}else{
if(status==BAD_OWNER){
if(IGNORE_BAD_OWNER){
if(bb.length()>k){bb.length--;}
}else{
release(bb, id);
// outstream.print("C1");
return null;
}
}else if(status==F_BRANCH || status==D_BRANCH){
leftRatio=calcRatio(rightCounts);
}else if(status==B_BRANCH){
leftRatio=calcRatio(leftCounts);
}else{
throw new RuntimeException("Bad return value: "+status);
}
}
leftStatus=status;
}
// outstream.print("D");
if(verbose /*|| true*/){outstream.println("A: Final length for thread "+id+": "+bb.length());}
// if(useOwnership && THREADS==1){assert(claim(bases, bases.length, id, rid));}
success=(useOwnership ? doubleClaim(bb, id) : true);
if(verbose /*|| true*/){outstream.println("Success for thread "+id+": "+success);}
if(trimEnds>0){bb.trimByAmount(trimEnds, trimEnds);}
else if(trimCircular && leftStatus==LOOP && rightStatus==LOOP){bb.trimByAmount(0, k-1);}
if(joinContigs || (bb.length()>=initialLength+minExtension && (bb.length()>=minContigLen || popBubbles))){
if(success){
bb.reverseComplementInPlace();
byte[] bases=bb.toBytes();
Contig c=new Contig(bases);
c.leftCode=leftStatus;
c.rightCode=rightStatus;
c.rightRatio=rightRatio;
c.leftRatio=leftRatio;
if(!c.canonical()){c.rcomp();}
return c;
}else{
// outstream.print("E");
// assert(false) : bb.length()+", "+id;
release(bb, id);
return null;
}
}
if(verbose /*|| true*/){outstream.println("A: Contig was too short for "+id+": "+bb.length());}
// assert(false) : bb.length()+", "+initialLength+", "+minExtension+", "+minContigLen;
// outstream.print("F");
return null;
}
/** From a seed */
private byte[] makeContig(final byte[] bases, final ByteBuilder bb, long rid){
if(bases==null || bases.length<k){return null;}
// if(verbose /*|| true*/){outstream.println("Thread "+id+" checking owner: "+findOwner(bases, bases.length, id));}
int owner=useOwnership ? findOwner(bases, bases.length, id) : -1;
if(owner>=id){return null;}
boolean success=(useOwnership ? claim(bases, bases.length, id, true) : true);
if(verbose /*|| true*/){outstream.println("Thread "+id+" checking owner after setting: "+findOwner(bases, bases.length, id));}
if(!success){
release(bases, bases.length, id);
return null;
}
if(verbose /*|| true*/){outstream.println("Thread "+id+" building contig; initial length "+bases.length);}
bb.setLength(0);
bb.append(bases);
if(verbose){outstream.println("Extending to right.");}
{
final int status=extendToRight(bb, leftCounts, rightCounts, id);
if(status==DEAD_END){
//do nothing
}else if(status==LOOP){//TODO
//special case - handle specially, for a loop with no obvious junction, e.g. long tandem repeat.
//Perhaps, the last kmer should be reclassified as a junction and removed.
}else if(status==BAD_SEED){
//do nothing
}else{
if(status==BAD_OWNER){
release(bb, id);
return null;
}else if(isBranchCode(status)){
//do nothing
}else{
throw new RuntimeException("Bad return value: "+status);
}
}
}
bb.reverseComplementInPlace();
if(verbose /*|| true*/){outstream.println("Extending rcomp to right; current length "+bb.length());}
{
final int status=extendToRight(bb, leftCounts, rightCounts, id);
if(status==DEAD_END){
//do nothing
}else if(status==LOOP){//TODO
//special case - handle specially, for a loop with no obvious junction, e.g. long tandem repeat.
//Perhaps, the last kmer should be reclassified as a junction and removed.
}else if(status==BAD_SEED){
//do nothing
}else{
if(status==BAD_OWNER){
release(bb, id);
return null;
}else if(isBranchCode(status)){
//do nothing
}else{
throw new RuntimeException("Bad return value: "+status);
}
}
}
if(verbose /*|| true*/){outstream.println("B: Final length for thread "+id+": "+bb.length());}
// if(useOwnership && THREADS==1){assert(claim(bases, bases.length, id, rid));}
success=(useOwnership ? doubleClaim(bb, id) : true);
if(verbose /*|| true*/){outstream.println("Success for thread "+id+": "+success);}
if(bb.length()>=bases.length+minExtension && (bb.length()>=minContigLen || popBubbles)){
if(success){
bb.reverseComplementInPlace();
return bb.toBytes();
}else{
// assert(false) : bb.length()+", "+id;
release(bb.array, bb.length(), id);
return null;
}
}
if(verbose /*|| true*/){outstream.println("B: Contig was too short for "+id+": "+bb.length());}
return null;
}
}
/*--------------------------------------------------------------*/
/*---------------- Contig Processing ----------------*/
/*--------------------------------------------------------------*/
@Override
ProcessContigThread makeProcessContigThread(ArrayList<Contig> contigs, AtomicInteger next){
return new ProcessContigThread(contigs, next);
}
@Override
public void initializeContigs(ArrayList<Contig> contigs){
tables.clearOwnership();
tables.initializeOwnership();
{
int cnum=0;
for(Contig c : contigs){
c.id=cnum;
// if(c.leftBranch()){
if(true){
long kmer=c.leftKmer(k);
tables.claim(kmer, rcomp(kmer), cnum);
}
// if(c.rightBranch()){
if(true){
long kmer=c.rightKmer(k);
tables.claim(kmer, rcomp(kmer), cnum);
}
cnum++;
}
}
}
class ProcessContigThread extends AbstractProcessContigThread {
ProcessContigThread(ArrayList<Contig> contigs_, AtomicInteger next_){
super(contigs_, next_);
lastExitCondition=BAD_SEED;
}
@Override
public void processContigLeft(Contig c, int[] leftCounts, int[] rightCounts, int[] extraCounts, ByteBuilder bb){
if(c.leftCode==DEAD_END){return;}
final long kmer0=c.leftKmer(k);
final long rkmer0=rcomp(kmer0);
assert(tables.getCount(kmer0, rkmer0)>0);
assert(tables.findOwner(kmer0)==c.id) : tables.findOwner(kmer0)+", "+c.id;
// System.err.println(tables.findOwner(kmer0));
int leftMaxPos=fillLeftCounts(kmer0, rkmer0, leftCounts);
int leftMax=leftCounts[leftMaxPos];
int leftSecondPos=Tools.secondHighestPosition(leftCounts);
int leftSecond=leftCounts[leftSecondPos];
for(int x=0; x<leftCounts.length; x++){
bb.clear();
final int count=leftCounts[x];
int target=-1;
if(count>0 && isJunction(leftMax, count)){
long x2=3-x;
long rkmer=((rkmer0<<2)|(long)x2)&mask;
long kmer=(kmer0>>>2)|(((long)x)<<shift2);
assert(kmer==rcomp(rkmer));
assert(tables.getCount(kmer, rkmer)==count) : count+", "+tables.getCount(kmer, rkmer);
bb.append(AminoAcid.numberToBase[x]);
target=exploreRight(rkmer, kmer, extraCounts, rightCounts, bb);
if(verbose){
outstream.println(c.id+"L_F: x="+x+", cnt="+count+", dest="+target
+", "+codeStrings[lastExitCondition]+", len="+lastLength+", orient="+lastOrientation);
}
}
if(target>=0){
Edge se=new Edge(c.id, target, lastLength, lastOrientation, count, bb.toBytes());
c.addLeftEdge(se);
edgesMadeT++;
}
}
}
@Override
public void processContigRight(Contig c, int[] leftCounts, int[] rightCounts, int[] extraCounts, ByteBuilder bb){
if(c.rightCode==DEAD_END){return;}
final long kmer0=c.rightKmer(k);
final long rkmer0=rcomp(kmer0);
int rightMaxPos=fillRightCounts(kmer0, rkmer0, rightCounts);
int rightMax=rightCounts[rightMaxPos];
int rightSecondPos=Tools.secondHighestPosition(rightCounts);
int rightSecond=rightCounts[rightSecondPos];
for(int x=0; x<rightCounts.length; x++){
bb.clear();
final int count=rightCounts[x];
int target=-1;
if(count>0 && isJunction(rightMax, count)){
long x2=3-x;
long kmer=((kmer0<<2)|(long)x)&mask;
long rkmer=(rkmer0>>>2)|(((long)x2)<<shift2);
assert(kmer==rcomp(rkmer));
assert(tables.getCount(kmer, rkmer)==count) : count+", "+tables.getCount(kmer, rkmer);
bb.append(AminoAcid.numberToBase[x]);
target=exploreRight(kmer, rkmer, leftCounts, extraCounts, bb);
if(verbose){
outstream.println(c.id+"R_F: x="+x+", cnt="+count+", dest="+target+", "+codeStrings[lastExitCondition]+", len="+lastLength+", orient="+lastOrientation);
}
}
if(target>=0){
lastOrientation|=1;
Edge se=new Edge(c.id, target, lastLength, lastOrientation, count, bb.toBytes());
c.addRightEdge(se);
edgesMadeT++;
}
}
}
private int exploreRight(long kmer, long rkmer, int[] leftCounts, int[] rightCounts, ByteBuilder bb){
int length=1;
int owner=-1;
lastTarget=-1;
for(; length<500; length++){
owner=tables.findOwner(kmer, rkmer);
if(owner>=0){break;}
final int leftMaxPos=fillLeftCounts(kmer, rkmer, leftCounts);
final int leftMax=leftCounts[leftMaxPos];
final int leftSecondPos=Tools.secondHighestPosition(leftCounts);
final int leftSecond=leftCounts[leftSecondPos];
if(isJunction(leftMax, leftSecond)){
lastExitCondition=B_BRANCH;
lastLength=length;
return -1;
}
final int rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
final int rightMax=rightCounts[rightMaxPos];
final int rightSecondPos=Tools.secondHighestPosition(rightCounts);
final int rightSecond=rightCounts[rightSecondPos];
// outstream.println("* "+Arrays.toString(leftCounts)+", "+Arrays.toString(rightCounts)+", "+rightMaxPos);
if(rightMax<minCountExtend){
// assert(false) : Arrays.toString(rightCounts);
lastExitCondition=DEAD_END;
lastLength=length;
return -1;
}else if(isJunction(rightMax, rightSecond)){
lastExitCondition=F_BRANCH;
lastLength=length;
return -1;
}
bb.append(AminoAcid.numberToBase[rightMaxPos]);
long x=rightMaxPos;
long x2=3-x;
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
}
lastLength=length;
lastTarget=owner;
if(owner>=0){
lastExitCondition=SUCCESS;
Contig dest=contigs.get(owner);
long left=dest.leftKmer(k);
// if(left==kmer){
// lastOrientation=0;
// }else if(left==rkmer){
// lastOrientation=1;
// }else{
// long right=dest.rightKmer(k);
// if(right==kmer){
// lastOrientation=2;
// }else if(right==rkmer){
// lastOrientation=3;
// }
// }
if(left==kmer || left==rkmer){
lastOrientation=0;
}else{
long right=dest.rightKmer(k);
if(right==kmer || right==rkmer){
lastOrientation=2;
}else{
assert(false);
}
}
}else{
lastExitCondition=TOO_LONG;
}
return owner;
}
}
/*--------------------------------------------------------------*/
/*---------------- Extension Methods ----------------*/
/*--------------------------------------------------------------*/
public int findInsertSize(Read r1, Read r2, int[] rightCounts){
final long kmer1=tables.rightmostKmer(r1.bases, r1.length());
final long kmer2=tables.rightmostKmer(r2.bases, r2.length());
if(kmer1<0 || kmer2<0){return -1;}
final long rkmer1=rcomp(kmer1);
final long rkmer2=rcomp(kmer2);
final int x=measureInsert(kmer1, rkmer1, kmer2, rkmer2, 24000, rightCounts);
if(x<0){return -1;}
return r1.length()+r2.length()+x-k;//TODO: May be off by 1.
}
@Override
public int extendRead(Read r, ByteBuilder bb, int[] leftCounts, int[] rightCounts, int distance, final Kmer kmer){
return extendRead(r, bb, leftCounts, rightCounts, distance);
}
@Override
public int extendRead(Read r, ByteBuilder bb, int[] leftCounts, int[] rightCounts, int distance){
final int initialLen=r.length();
if(initialLen<k){return 0;}
bb.setLength(0);
bb.append(r.bases);
final int extension=extendToRight2(bb, leftCounts, rightCounts, distance, true);
if(extension>0){
r.bases=bb.toBytes();
if(r.quality!=null){
final byte q=Shared.FAKE_QUAL;
r.quality=KillSwitch.copyOf(r.quality, r.bases.length);
for(int i=initialLen; i<r.quality.length; i++){
r.quality[i]=q;
}
}
}
assert(extension==r.length()-initialLen);
return extension;
}
/** Returns distance between the two kmers, or -1 */
public int measureInsert(final long kmer1, final long rkmer1, final long kmer2, final long rkmer2, final int maxlen, final int[] rightCounts){
final long key2=toValue(kmer2, rkmer2);
long kmer=kmer1;
long rkmer=rkmer1;
int len=0;
{
int count=tables.getCount(key2);
if(count<minCountSeed){return -1;}
}
long key=toValue(kmer, rkmer);
int count=tables.getCount(key);
if(count<minCountSeed){return -1;}
if(count<minCountSeed){
if(verbose){outstream.println("Returning because count was too low: "+count);}
return -1;
}
int rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
int rightMax=rightCounts[rightMaxPos];
// int rightSecondPos=Tools.secondHighestPosition(rightCounts);
// int rightSecond=rightCounts[rightSecondPos];
if(rightMax<minCountExtend){return -1;}
// if(isJunction(rightMax, rightSecond)){return -1;}
while(key!=key2 && len<maxlen){
//Generate the new kmer
// final byte b=AminoAcid.numberToBase[rightMaxPos];
final long x=rightMaxPos;
final long x2=AminoAcid.numberToComplement[(int)x];
//Now consider the next kmer
kmer=((kmer<<2)|(long)x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
assert(tables.getCount(kmer, rkmer)==rightMax);
count=rightMax;
assert(count>=minCountExtend) : count;
rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
rightMax=rightCounts[rightMaxPos];
// rightSecondPos=Tools.secondHighestPosition(rightCounts);
// rightSecond=rightCounts[rightSecondPos];
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+Arrays.toString(rightCounts));
outstream.println("rightMaxPos="+rightMaxPos);
outstream.println("rightMax="+rightMax);
// outstream.println("rightSecondPos="+rightSecondPos);
// outstream.println("rightSecond="+rightSecond);
}
if(rightMax<minCountExtend){
if(verbose){outstream.println("A: Breaking because highest right was too low:"+rightMax);}
break;
}
// if(isJunction(rightMax, rightSecond)){return -1;}
len++;
}
return len>=maxlen ? -1 : len;
}
/**
* Extend these bases into a contig.
* Stops at both left and right junctions.
* Claims ownership.
*/
public int extendToRight(final ByteBuilder bb, final int[] leftCounts, final int[] rightCounts, final int id){
if(bb.length()<k){return BAD_SEED;}
long kmer=0;
long rkmer=0;
int len=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
{
final int bblen=bb.length();
final byte[] bases=bb.array;
for(int i=bblen-k; i<bblen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{len++;}
if(verbose){outstream.println("A: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
}
if(len<k){return BAD_SEED;}
else{assert(len==k);}
/* Now the trailing kmer has been initialized. */
long key=toValue(kmer, rkmer);
HashArray1D table=tables.getTableForKey(key);
int count=table.getValue(key);
if(count<minCountSeed){
if(verbose){outstream.println("Returning because count was too low: "+count);}
return BAD_SEED;
}
int owner=(useOwnership ? table.getOwner(key) : id);
if(verbose){outstream.println("Owner: "+owner);}
if(owner>id){return BAD_OWNER;}
int leftMaxPos=0;
int leftMax=minCountExtend;
int leftSecondPos=1;
int leftSecond=0;
if(leftCounts!=null){
leftMaxPos=fillLeftCounts(kmer, rkmer, leftCounts);
leftMax=leftCounts[leftMaxPos];
leftSecondPos=Tools.secondHighestPosition(leftCounts);
leftSecond=leftCounts[leftSecondPos];
}
int rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
int rightMax=rightCounts[rightMaxPos];
int rightSecondPos=Tools.secondHighestPosition(rightCounts);
int rightSecond=rightCounts[rightSecondPos];
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+(leftCounts==null ? "null" : Arrays.toString(leftCounts))+", "+Arrays.toString(rightCounts));
outstream.println("leftMaxPos="+leftMaxPos);
outstream.println("leftMax="+leftMax);
outstream.println("leftSecondPos="+leftSecondPos);
outstream.println("leftSecond="+leftSecond);
outstream.println("rightMaxPos="+rightMaxPos);
outstream.println("rightMax="+rightMax);
outstream.println("rightSecondPos="+rightSecondPos);
outstream.println("rightSecond="+rightSecond);
}
if(rightMax<minCountExtend){return DEAD_END;}
if(isJunction(rightMax, rightSecond)){//Returning here is fine because nothing can be added
if(verbose){outstream.println("B: Breaking because isJunction("+rightMax+", "+rightSecond+", "+leftMax+", "+leftSecond+")");}
return isJunction(leftMax, leftSecond) ? D_BRANCH : F_BRANCH;
}
if(isJunction(leftMax, leftSecond)){//Returning here is necessary, but this should mean the the length is exactly K
assert(bb.length()==k) : bb.length()+", "+k+", "+leftMax+", "+leftSecond;
if(verbose){outstream.println("B: Breaking because isJunction("+rightMax+", "+rightSecond+", "+leftMax+", "+leftSecond+")");}
return B_BRANCH;
}
if(useOwnership){
owner=table.setOwner(key, id);
if(verbose){outstream.println("A. Owner is now "+id+" for key "+key);}
if(owner!=id){
if(verbose){outstream.println("Returning early because owner was "+owner+" for thread "+id+".");}
return BAD_OWNER;
}
}
final int maxLen=Tools.min((extendRight<0 ? maxContigLen : bb.length()+extendRight), maxContigLen);
while(owner==id && bb.length()<maxLen){
//Generate the new kmer
final byte b=AminoAcid.numberToBase[rightMaxPos];
final long x=rightMaxPos;
final long x2=AminoAcid.numberToComplement[(int)x];
final long evicted=(kmer>>>shift2); //Binary value that falls off the end.
//Now consider the next kmer
kmer=((kmer<<2)|(long)x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
key=toValue(kmer, rkmer);
table=tables.getTableForKey(key);
assert(table.getValue(key)==rightMax || rightMax==0) : key+", "+table.getValue(key)+", "+rightMax+", "+Arrays.toString(rightCounts);
count=rightMax;
assert(count>=minCountExtend) : count;
if(leftCounts!=null){
leftMaxPos=fillLeftCounts(kmer, rkmer, leftCounts);
leftMax=leftCounts[leftMaxPos];
leftSecondPos=Tools.secondHighestPosition(leftCounts);
leftSecond=leftCounts[leftSecondPos];
}
rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
rightMax=rightCounts[rightMaxPos];
rightSecondPos=Tools.secondHighestPosition(rightCounts);
rightSecond=rightCounts[rightSecondPos];
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+(leftCounts==null ? "null" : Arrays.toString(leftCounts))+", "+Arrays.toString(rightCounts));
outstream.println("leftMaxPos="+leftMaxPos);
outstream.println("leftMax="+leftMax);
outstream.println("leftSecondPos="+leftSecondPos);
outstream.println("leftSecond="+leftSecond);
outstream.println("rightMaxPos="+rightMaxPos);
outstream.println("rightMax="+rightMax);
outstream.println("rightSecondPos="+rightSecondPos);
outstream.println("rightSecond="+rightSecond);
}
final boolean fbranch=isJunction(rightMax, rightSecond);
final boolean bbranch=isJunction(leftMax, leftSecond);
final boolean hbranch=(leftCounts!=null && leftMaxPos!=evicted && branchMult1>0);
if(bbranch){
if(verbose){outstream.println("B: Breaking - isJunction("+rightMax+", "+rightSecond+", "+leftMax+", "+leftSecond+"); "
+ "("+fbranch+", "+bbranch+", "+hbranch+")");}
return fbranch ? D_BRANCH : B_BRANCH;
}else if(hbranch){
if(verbose){outstream.println("B: Breaking - isJunction("+rightMax+", "+rightSecond+", "
+ ""+leftMax+", "+leftSecond+"); ("+fbranch+", "+bbranch+", "+hbranch+")");}
if(verbose){outstream.println("Hidden branch: leftMaxPos!=evicted ("+leftMaxPos+"!="+evicted+")" +
"\nleftMaxPos="+leftMaxPos+", leftMax="+leftMax+", leftSecondPos="+leftSecondPos+", leftSecond="+leftSecond);}
return fbranch ? D_BRANCH : B_BRANCH;
}
bb.append(b);
if(verbose){outstream.println("Added base "+(char)b);}
if(useOwnership){
owner=table.getOwner(key);
if(verbose){outstream.println("Owner is initially "+id+" for key "+key);}
if(owner==id){//loop detection
if(verbose /*|| true*/){
// outstream.println(new String(bb.array, bb.length()-31, 31));
outstream.println(bb);
outstream.println(toText(kmer));
outstream.println(toText(rkmer));
outstream.println("Breaking because owner was "+owner+" for thread "+id+".");
}
return fbranch ? F_BRANCH : LOOP;
}
owner=table.setOwner(key, id);
if(verbose){outstream.println("B. Owner is now "+id+" for key "+key);}
}
if(fbranch){
if(verbose){outstream.println("B: Breaking - isJunction("+rightMax+", "+rightSecond+", "+leftMax+", "+leftSecond+"); "
+ "("+fbranch+", "+bbranch+", "+hbranch+")");}
return F_BRANCH;
}else if(rightMax<minCountExtend){
if(verbose){outstream.println("B: Breaking because highest right was too low:"+rightMax);}
return DEAD_END;
}
}
assert(owner!=id);
if(verbose /*|| true*/){
outstream.println("Current contig: "+bb+"\nReturning because owner was "+owner+" for thread "+id+".");
}
return BAD_OWNER;
}
@Override
public int extendToRight2(final ByteBuilder bb, final int[] leftCounts, final int[] rightCounts, final int distance, boolean includeJunctionBase){
if(verbose || verbose2){outstream.println("Entering extendToRight2 (no kmers).");}
final int initialLength=bb.length();
if(initialLength<k){return 0;}
long kmer=0;
long rkmer=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the rightmost kmer */
{
int len=0;
final byte[] bases=bb.array;
for(int i=initialLength-k; i<initialLength; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{len++;}
if(verbose){outstream.println("B: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
if(len<k){
if(verbose || verbose2){outstream.println("Returning because len<k: "+len+"<"+k);}
return 0;
}
else{assert(len==k);}
}
return extendToRight2(bb, leftCounts, rightCounts, distance, includeJunctionBase, kmer, rkmer);
}
/**
* Extend these bases to the right by at most 'distance'.
* Stops at right junctions only.
* Does not claim ownership.
*/
public int extendToRight2(final ByteBuilder bb, final int[] leftCounts, final int[] rightCounts, final int distance, boolean includeJunctionBase,
long kmer, long rkmer){
if(verbose || verbose2){outstream.println("Entering extendToRight2 (with kmers).");}
final int initialLength=bb.length();
/* Now the trailing kmer has been initialized. */
long key=toValue(kmer, rkmer);
HashArray1D table=tables.getTableForKey(key);
int count=table.getValue(key);
if(count<minCountSeed){
if(verbose || verbose2){outstream.println("Returning because count was too low: "+count+"<"+minCountSeed);}
return 0;
}
int leftMaxPos=0;
int leftMax=minCountExtend;
int leftSecondPos=1;
int leftSecond=0;
if(leftCounts!=null){
leftMaxPos=fillLeftCounts(kmer, rkmer, leftCounts);
leftMax=leftCounts[leftMaxPos];
leftSecondPos=Tools.secondHighestPosition(leftCounts);
leftSecond=leftCounts[leftSecondPos];
}
int rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
int rightMax=rightCounts[rightMaxPos];
int rightSecondPos=Tools.secondHighestPosition(rightCounts);
int rightSecond=rightCounts[rightSecondPos];
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+Arrays.toString(rightCounts));
outstream.println("rightMaxPos="+rightMaxPos);
outstream.println("rightMax="+rightMax);
outstream.println("rightSecondPos="+rightSecondPos);
outstream.println("rightSecond="+rightSecond);
}
if(rightMax<minCountExtend){
if(verbose || verbose2){outstream.println("Returning because rightMax was too low: "+rightMax+"<"+minCountExtend+"\n"+count+", "+Arrays.toString(rightCounts));}
return 0;
}
if(isJunction(rightMax, rightSecond, leftMax, leftSecond)){
if(verbose || verbose2){outstream.println("Returning because isJunction: "+rightMax+", "+rightSecond+"; "+leftMax+", "+leftSecond);}
return 0;
}
final int maxLen=Tools.min(bb.length()+distance, maxContigLen);
while(bb.length()<maxLen){
//Generate the new kmer
final byte b=AminoAcid.numberToBase[rightMaxPos];
final long x=rightMaxPos;
final long x2=AminoAcid.numberToComplement[(int)x];
final long evicted=(kmer>>>shift2); //Binary value that falls off the end.
//Now consider the next kmer
kmer=((kmer<<2)|(long)x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
key=toValue(kmer, rkmer);
table=tables.getTableForKey(key);
assert(table.getValue(key)==rightMax || rightMax==0);
count=rightMax;
assert(count>=minCountExtend) : count;
if(leftCounts!=null){
leftMaxPos=fillLeftCounts(kmer, rkmer, leftCounts);
leftMax=leftCounts[leftMaxPos];
leftSecondPos=Tools.secondHighestPosition(leftCounts);
leftSecond=leftCounts[leftSecondPos];
}
rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
rightMax=rightCounts[rightMaxPos];
rightSecondPos=Tools.secondHighestPosition(rightCounts);
rightSecond=rightCounts[rightSecondPos];
if(verbose){
outstream.println("kmer: "+toText(kmer)+", "+toText(rkmer));
outstream.println("Counts: "+count+", "+Arrays.toString(rightCounts));
outstream.println("rightMaxPos="+rightMaxPos);
outstream.println("rightMax="+rightMax);
outstream.println("rightSecondPos="+rightSecondPos);
outstream.println("rightSecond="+rightSecond);
}
if(isJunction(rightMax, rightSecond, leftMax, leftSecond)){
if(verbose){outstream.println("B: Breaking because isJunction("+rightMax+", "+rightSecond+", "+leftMax+", "+leftSecond+")");}
if(includeJunctionBase && kmer>rkmer){
bb.append(b);
if(verbose){outstream.println("Added base "+(char)b);}
}
break;
}
if(leftCounts!=null && leftMaxPos!=evicted){
if(verbose){outstream.println("B: Breaking because of hidden branch: leftMaxPos!=evicted ("+leftMaxPos+"!="+evicted+")" +
"\nleftMaxPos="+leftMaxPos+", leftMax="+leftMax+", leftSecondPos="+leftSecondPos+", leftSecond="+leftSecond);}
if(includeJunctionBase && kmer>rkmer){
bb.append(b);
if(verbose){outstream.println("Added base "+(char)b);}
}
break;
}
bb.append(b);
if(verbose){outstream.println("Added base "+(char)b);}
if(rightMax<minCountExtend){
if(verbose || verbose2){outstream.println("C: Breaking because highest right was too low: "+rightMax+"<"+minCountExtend);}
break;
}
}
if(verbose || verbose2){outstream.println("Extended by "+(bb.length()-initialLength));}
return bb.length()-initialLength;
}
/*--------------------------------------------------------------*/
/*---------------- Junk Detection ----------------*/
/*--------------------------------------------------------------*/
@Override
public boolean isJunk(Read r){
boolean junk=isJunk(r, localRightCounts.get());
return junk;
}
@Override
public boolean isJunk(Read r, final int[] counts, Kmer kmer){
return isJunk(r, counts);
}
public boolean isJunk(Read r, final int[] counts){
final int blen=r.length();
if(blen<k){return true;}
final byte[] bases=r.bases;
long kmer=0;
long rkmer=0;
int len=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the leftmost kmer */
{
for(int i=0; i<k; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{len++;}
if(verbose){outstream.println("C: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
}
if(len>=k){
int maxPos=fillLeftCounts(kmer, rkmer, counts);
if(counts[maxPos]>0){
//outstream.println("Not junk, left counts[maxPos]="+counts[maxPos]+"; right="+fillRightCounts(kmer, rkmer, counts, mask, shift2));
return false;
}
}
final boolean paired=(r.mateLength()>=k);
int maxDepth=0;
{
for(int i=k; i<blen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{
len++;
if(len>=k){
int depth=getCount(kmer, rkmer);
if(depth>maxDepth){
maxDepth=depth;
if(maxDepth>1 && (!paired || maxDepth>2)){
//outstream.println("Not junk, maxDepth="+maxDepth);
return false;
}
}
}
}
if(verbose){outstream.println("D: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
}
if(len>=k && !paired){
int maxPos=fillRightCounts(kmer, rkmer, counts);
if(counts[maxPos]>0){
//outstream.println("Not junk, right counts[maxPos]="+counts[maxPos]);
return false;
}
}
return true;
}
@Override
public boolean hasKmersAtOrBelow(Read r, int tooLow, final float fraction, Kmer kmer){
return hasKmersAtOrBelow(r, tooLow, fraction);
}
@Override
public boolean hasKmersAtOrBelow(Read r, final int tooLow, final float fraction){
final int blen=r.length();
if(blen<k){return true;}
final byte[] bases=r.bases;
// outstream.println("\n"+new String(r.bases)+":");
long kmer=0;
long rkmer=0;
int len=0;
final int limit=Tools.max(1, Math.round((bases.length-kbig+1)*fraction));
int valid=0, invalid=0;
/* Loop through the bases, maintaining a forward and reverse kmer via bitshifts, to get the leftmost kmer */
{
for(int i=0; i<blen; i++){
final byte b=bases[i];
final long x=AminoAcid.baseToNumber[b];
final long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x<0){
len=0;
kmer=rkmer=0;
}else{
len++;
if(len>=k){
int depth=getCount(kmer, rkmer);
// outstream.println("depth="+depth+", kmer="+toText(kmer));
if(depth>tooLow){valid++;}
else{
invalid++;
if(invalid>=limit){return true;}
}
}
}
if(verbose){outstream.println("E: Scanning i="+i+", len="+len+", kmer="+kmer+", rkmer="+rkmer+"\t"+new String(bases, Tools.max(0, i-k2), Tools.min(i+1, k)));}
}
}
//Compensate for nocalls changing the expected kmer count
final int limit2=Tools.max(1, Math.round((valid+invalid)*fraction));
return valid<1 || invalid>=limit2;
}
/*--------------------------------------------------------------*/
/*---------------- Error Correction ----------------*/
/*--------------------------------------------------------------*/
@Override
public int errorCorrect(Read r){
initializeThreadLocals();
int corrected=errorCorrect(r, localLeftCounts.get(), localRightCounts.get(), localLongList.get(),
localIntList.get(), localIntList2.get(), localByteBuilder.get(), localByteBuilder2.get(), localTracker.get(), localBitSet.get());
return corrected;
}
@Override
public int errorCorrect(Read r, final int[] leftCounts, final int[] rightCounts, LongList kmers, IntList counts, IntList counts2,
final ByteBuilder bb, final ByteBuilder bb2, final ErrorTracker tracker, final BitSet bs, Kmer kmer, Kmer kmer2){
return errorCorrect(r, leftCounts, rightCounts, kmers, counts, counts2, bb, bb2, tracker, bs);
}
boolean hasErrorsFast(LongList kmers){
if(kmers.size<1){return false;}
int prev=-1;
final int incr=Tools.mid(1, k/2, 9), mcc=minCountCorrect();
for(int i=0; i<kmers.size; i+=incr){
long kmer=kmers.get(i);
if(kmer<0){
return true;
}
long rkmer=rcomp(kmer);
int count=getCount(kmer, rkmer);
final int min=Tools.min(count, prev), max=Tools.max(count, prev);
if(count<mcc || (i>0 && (isError(max+1, min-1)))){return true;}
prev=count;
}
long kmer=kmers.get(kmers.size()-1);
if(kmer<0){return true;}
long rkmer=rcomp(kmer);
int count=getCount(kmer, rkmer);
final int min=Tools.min(count, prev), max=Tools.max(count, prev);
return count<mcc || isError(max+1, min-1);
}
public int errorCorrect(Read r, final int[] leftCounts, final int[] rightCounts, LongList kmers, IntList counts, IntList counts2,
final ByteBuilder bb, final ByteBuilder bb2, final ErrorTracker tracker, final BitSet bs){
final byte[] bases=r.bases;
final byte[] quals=r.quality;
tracker.clear();
int valid=tables.fillKmers(bases, kmers);
if(valid<2){return 0;}
if(!r.containsUndefined() && !hasErrorsFast(kmers)){return 0;}
tables.fillCounts(kmers, counts);
final int possibleErrors=tracker.suspected=countErrors(counts, quals);
if(possibleErrors<0){return 0;}
final float expectedErrors=r.expectedErrors(true, r.length());
final Rollback roll=ECC_ROLLBACK ? new Rollback(r, counts) : null;
assert(counts.size>0);
int correctedPincer=0;
int correctedTail=0;
int correctedBrute=0;
int correctedReassemble=0;
if(ECC_PINCER){
correctedPincer+=errorCorrectPincer(bases, quals, leftCounts, rightCounts, kmers, counts, bb, tracker, errorExtensionPincer);
}
if(ECC_TAIL || ECC_ALL){
int start=(ECC_ALL ? 0 : counts.size-k-1);
// if(ECC_PINCER && tracker!=null && tracker.detected>correctedPincer){start=start-k;}
correctedTail+=errorCorrectTail(bases, quals, leftCounts, rightCounts, kmers, counts, bb, tracker, start, errorExtensionTail);
r.reverseComplement();
valid=tables.fillKmers(bases, kmers);
counts.reverse();
correctedTail+=errorCorrectTail(bases, quals, leftCounts, rightCounts, kmers, counts, bb, tracker, start, errorExtensionTail);
r.reverseComplement();
counts.reverse();
}
if(ECC_REASSEMBLE){
if(verbose){outstream.println("Correcting "+possibleErrors+" errors. Counts:\n"+counts);}
if((correctedPincer<1 && correctedTail<1) || countErrors(counts, quals)>0){
correctedReassemble=reassemble(bases, quals, rightCounts, counts, counts2, tracker, errorExtensionReassemble, bb, bb2, null, null, bs);
}
// assert(tracker.detectedReassemble>0) : counts;
if(verbose){outstream.println("Corrected "+correctedReassemble+" errors. Counts:\n"+counts);}
}
assert(counts.size>0);
//123 For testing.
if(false && tracker.detected()>tracker.corrected()){
correctedBrute+=errorCorrectBruteForce(bases, quals, leftCounts, rightCounts, kmers, counts, bb, tracker, errorExtensionPincer);
}
assert(correctedPincer+correctedTail+correctedReassemble+correctedBrute==tracker.corrected())
: correctedPincer+", "+correctedTail+", "+correctedReassemble+", "+correctedBrute+", "+tracker;
if(ECC_ROLLBACK && (tracker.corrected()>0 || tracker.rollback)){
if(!tracker.rollback && quals!=null && tracker.corrected()>3){
float mult=Tools.max(1, 0.5f*(0.5f+0.01f*r.length()));//1 for a 150bp read.
if(countErrors(counts, quals)>0 && tracker.corrected()>mult+expectedErrors){tracker.rollback=true;}
else if(tracker.corrected()>2.5f*mult+expectedErrors){tracker.rollback=true;}
}
// boolean printed=false;
IntList counts0=roll.counts0;
for(int i=0; !tracker.rollback && i<counts.size; i++){
int a=Tools.max(0, counts0.get(i));
int b=Tools.max(0, counts.get(i));
// assert(b+1>=a) : "Z: RID="+r.numericID+"; "+a+"->"+b+"\n"+counts0+"\n"+counts;
if(b<a-1 && !isSimilar(a, b)){
// assert(false) : "Y: RID="+r.numericID+"; "+a+"->"+b+"\n"+counts0+"\n"+counts;
if(verbose){outstream.println("Y: RID="+r.numericID+"; "+a+"->"+b+"\n"+counts0+"\n"+counts);}
tracker.rollback=true;
}
// else if(b<a-1 && !printed){
// assert(false);
// if(verbose){outstream.println("X: RID="+r.numericID+"; "+a+"->"+b+"\n"+counts0+"\n"+counts);}
// printed=true;
// }
}
if(tracker.rollback){
roll.rollback(r, counts);
tracker.clearCorrected();
return 0;
}
}
if(MARK_BAD_BASES>0 && (!MARK_ERROR_READS_ONLY || countErrors(counts, quals)>0 ||
r.expectedErrors(false, r.length())>3)){
int marked=markBadBases(bases, quals, counts, bs, MARK_BAD_BASES, MARK_DELTA_ONLY, MARK_QUALITY);
tracker.marked=marked;
}
return tracker.corrected();
}
private int findBestMutant(final byte[] bases, final int a, final LongList kmers, final long[] bestMutant){
Arrays.fill(bestMutant, -1);
final long kmer0=kmers.get(a);
if(kmer0<0){return -1;}
long mask=3;
int max=0, second=0;
for(int i=0; i<k; i++){
for(long num=0; num<4; num++){
final long kmer=(kmer0&~mask)|(num<<(2*i));
if(kmer!=kmer0){
final long rkmer=rcomp(kmer);
int count=getCount(kmer, rkmer);
if(count>max){
second=max;
max=count;
bestMutant[0]=kmer;
bestMutant[1]=rkmer;
bestMutant[2]=i;
}else if(count>second){
second=count;
}
}
}
mask<<=2;
}
bestMutant[3]=max;
bestMutant[4]=second;
return max>second ? max : -1;
}
private boolean fixBestMutant(final byte[] bases, final int a, final LongList kmers, final IntList counts, final int pos, final long kmer){
int shift=2*pos;
long mask=(3L)<<shift;
byte num=(byte)((kmer>>shift)&mask);
byte base=AminoAcid.numberToBase[num];
byte old=bases[a+pos];
bases[a+pos]=base;
// long[] copy=counts.toArray();
// tables.regenerateKmers(bases, kmers, counts, a);
tables.fillKmers(bases, kmers);
tables.fillCounts(kmers, counts);
// for(int i=0; i<copy.length; i++){
// if(counts.get(i)<copy[i]){
// bases[a+pos]=old;
// tables.fillKmers(bases, kmers);
// tables.fillCounts(kmers, counts);
// return false;
// }
// }
return true;
}
public int errorCorrectBruteForce(final byte[] bases, final byte[] quals, final int[] leftBuffer, final int[] rightBuffer,
final LongList kmers, final IntList counts, final ByteBuilder bb, final ErrorTracker tracker, final int errorExtension){
int detected=0;
int corrected=0;
final long[] bestMutant=new long[5];
final int[] countCopy=counts.toArray();
final byte[] basesCopy=bases.clone();
final int maxCount=Tools.max(counts.array, 0, counts.size-1);
if(maxCount<minCountCorrect()){
return 0;
}
int thresh=Tools.max(3, maxCount/4);
for(int a=0, d=k+1; a<counts.size; a++, d++){
final int aCount=counts.get(a);
if(aCount<thresh){
detected++;
boolean fixed=false;
int mCount=findBestMutant(bases, a, kmers, bestMutant);
if(mCount>thresh && mCount>=minCountCorrect() && (isSimilar(mCount, maxCount) || (mCount<maxCount && mCount>(1+aCount)*3))){
long kmer=bestMutant[0];
long rkmer=bestMutant[1];
int pos=(int)bestMutant[2];
assert(mCount==bestMutant[3]);
int secondBestCount=(int)bestMutant[4];
if(mCount>(1+secondBestCount*3) && pos>=errorExtension && pos<=(k-errorExtension)){
fixBestMutant(bases, a, kmers, counts, pos, kmer);
fixed=true;
corrected++;
}
}
}
}
for(int i=0; i<countCopy.length; i++){
if(counts.get(i)<countCopy[i]){
for(int j=0; j<bases.length; j++){bases[j]=basesCopy[j];}
counts.clear();
for(int count : countCopy){counts.add(count);}
tables.fillKmers(bases, kmers);
corrected=0;
}
}
{
tracker.detectedBrute+=detected;
tracker.correctedBrute+=corrected;
}
return corrected;
}
public int errorCorrectPincer(final byte[] bases, final byte[] quals, final int[] leftBuffer, final int[] rightBuffer,
final LongList kmers, final IntList counts, final ByteBuilder bb, final ErrorTracker tracker, final int errorExtension){
int detected=0;
int corrected=0;
//a is the index of the left kmer
//b is a+1 (right-extension of left kmer)
//c is d-1 (left-extension of right kmer)
//d is the index of the right kmer
//the base between the kmers is at a+k
for(int a=0, d=k+1; d<counts.size; a++, d++){
final int aCount=counts.get(a);
final int bCount=counts.get(a+1);
final int cCount=counts.get(d-1);
final int dCount=counts.get(d);
final byte qb=(quals==null ? 20 : quals[a+kbig]);
if(isError(aCount, bCount, qb) && isError(dCount, cCount, qb) && isSimilar(aCount, dCount)){
if(verbose){
outstream.println("Found error: "+aCount+", "+bCount+", "+cCount+", "+dCount);
}
//Looks like a 1bp substitution; attempt to correct.
detected++;
int ret=correctSingleBasePincer(a, d, bases, quals, leftBuffer, rightBuffer, kmers, counts, bb, errorExtension);
corrected+=ret;
if(verbose){
outstream.println("Corrected error.");
}
}else{
if(verbose){
outstream.println("Not an error: "+aCount+", "+bCount+", "+cCount+", "+dCount+
"; "+isError(aCount, bCount, qb)+", "+isError(dCount, cCount, qb)+", "+isSimilar(aCount, dCount));
}
}
}
// if(detected==0 && counts.get(0)>2 && counts.get(counts.size-1)>2){
// assert(!verbose);
// verbose=true;
// outstream.println("\n"+counts);
// errorCorrectPincer(bases, quals, leftBuffer, rightBuffer, kmers, counts, bb, tracker);
// assert(false);
// }
{
tracker.detectedPincer+=detected;
tracker.correctedPincer+=corrected;
}
return corrected;
}
public int errorCorrectTail(final byte[] bases, final byte[] quals, final int[] leftBuffer, final int[] rightBuffer,
final LongList kmers, final IntList counts, final ByteBuilder bb, final ErrorTracker tracker, final int startPos, final int errorExtension){
if(bases.length<k+2+errorExtension+deadZone){return 0;}
int detected=0;
int corrected=0;
//a is the index of the left kmer
//b is a+1
//the base between the kmers is at a+k
for(int a=Tools.max(startPos, errorExtension), lim=counts.size-deadZone-1; a<lim; a++){//errorExtension-1
final int aCount=counts.get(a);
final int bCount=counts.get(a+1);
final byte qb=(quals==null ? 20 : quals[a+kbig]);
if(isError(aCount, bCount, qb) && isSimilar(aCount, a-errorExtension, a-1, counts) && isError(aCount, a+2, a+k, counts)){
if(verbose){
outstream.println("Found error: "+aCount+", "+bCount);
}
//Assume like a 1bp substitution; attempt to correct.
detected++;
int ret=correctSingleBaseRight(a, bases, quals, leftBuffer, rightBuffer, kmers, counts, bb, errorExtension);
corrected+=ret;
if(verbose){
outstream.println("Corrected error.");
}
}else{
if(verbose){
outstream.println("Not an error: "+aCount+", "+bCount+
"; "+isError(aCount, bCount, qb)+", "+isSimilar(aCount, a-errorExtension, a-1, counts)+", "+isError(aCount, a+2, a+k, counts));
}
}
}
// if(detected==0 && counts.get(0)>2 && counts.get(counts.size-1)>2){
// assert(!verbose);
// verbose=true;
// outstream.println("\n"+counts);
// errorCorrectPincer(bases, quals, leftBuffer, rightBuffer, kmers, counts, bb, tracker);
// assert(false);
// }
{
tracker.detectedTail+=detected;
tracker.correctedTail+=corrected;
}
return corrected;
}
// public int reassemble_inner(final ByteBuilder bases, final byte[] quals, final int[] rightCounts, final IntList counts,
// final int errorExtension, final Kmer kmer, final Kmer kmer2){
// throw new RuntimeException("TODO");
// }
@Override
public int reassemble_inner(final ByteBuilder bb, final byte[] quals, final int[] rightCounts, final IntList counts,
final int errorExtension, final Kmer kmer, final Kmer regenKmer){
return reassemble_inner(bb, quals, rightCounts, counts, errorExtension);
}
public int reassemble_inner(final ByteBuilder bb, final byte[] quals, final int[] rightCounts, final IntList counts,
final int errorExtension){
final int length=bb.length();
if(length<k+1+deadZone){return 0;}
final byte[] bases=bb.array;
long kmer=0, rkmer=0;
int detected=0;
int corrected=0;
int len=0;
//a is the index of the first base of the left kmer
//b=a+1 is the index of the next base
//ca=a-k is the index of the next base
//cb=a-k is the index of the next base
//the base between the kmers is at a+k
for(int a=0, lim=length-deadZone-1; a<lim; a++){
//Generate the new kmer
final byte aBase=bases[a];
final long x=AminoAcid.baseToNumber[aBase];
final long x2=AminoAcid.baseToComplementNumber[aBase];
if(x<0){
len=0;
kmer=rkmer=0;
}else{
//Now consider the next kmer
kmer=((kmer<<2)|(long)x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
len++;
if(verbose){
outstream.println("len: "+len+" vs "+k+"; a="+a);
}
if(len>=k){
final int b=a+1;
final int ca=a-k+1;
final int cb=ca+1;
final int aCount=counts.get(ca);
final int bCount=counts.get(cb);
final byte qb=(quals==null ? 20 : quals[b]);
if(verbose){
outstream.println("ca="+ca+", cb="+cb+"; aCount="+aCount+", bCount="+bCount);
outstream.println(isError(aCount, bCount, qb)+", "+isSimilar(aCount, ca-errorExtension, ca-1, counts)+
", "+isError(aCount, ca+2, ca+k, counts));
}
// if(isError(aCount, bCount) && isSimilar(aCount, ca-errorExtension, ca-1, counts) && isError(aCount, ca+2, ca+k, counts)){
if(isSubstitution(ca, errorExtension, qb, counts)){
if(verbose){
outstream.println("***Found error: "+aCount+", "+bCount);
}
//Assume a 1bp substitution; attempt to correct.
int rightMaxPos=fillRightCounts(kmer, rkmer, rightCounts);
int rightMax=rightCounts[rightMaxPos];
int rightSecondPos=Tools.secondHighestPosition(rightCounts);
int rightSecond=rightCounts[rightSecondPos];
byte base=bases[b];
byte num=AminoAcid.baseToNumber[base];
if(rightMax>=minCountExtend){
detected++;
if(num==rightMax){
detected--;
// bases2[b]=base;
}else if((isError(rightMax, rightSecond, qb) || !isJunction(rightMax, rightSecond)) && isSimilar(aCount, rightMax)){
bases[b]=AminoAcid.numberToBase[rightMaxPos];
corrected++;
tables.regenerateCounts(bases, counts, ca);
if(verbose){outstream.println("Corrected error: "+num+"->"+rightMaxPos+". New counts:\n"+counts);}
}
// else if(rightSecond>=minCountExtend && isJunction(rightMax, rightSecond) && isSimilar(aCount, rightSecond)
// && !isSimilar(aCount, rightMax)){//This branch may not be very safe.
// bases2[b]=AminoAcid.numberToBase[rightSecondPos];
// corrected++;
// if(verbose){outstream.println("Corrected error.");}
// }
}
}else{
if(verbose){
outstream.println("Not an error: "+aCount+", "+bCount+
"; "+isError(aCount, bCount, qb)+", "+isSimilar(aCount, a-errorExtension, a-1, counts)+", "+isError(aCount, a+2, a+k, counts));
}
}
}
}
}
return corrected;
}
private int correctSingleBasePincer(final int a, final int d, final byte[] bases, final byte[] quals, final int[] leftBuffer, final int[] rightBuffer,
final LongList kmers, final IntList counts, final ByteBuilder bb, final int errorExtension){
final byte leftReplacement, rightReplacement;
final int loc=a+k;
{
bb.clear();
final long kmer=kmers.get(a);
final long rkmer=rcomp(kmer);
int extension=extendToRight2(bb, null, rightBuffer, errorExtension, true, kmer, rkmer);
if(extension<errorExtension){return 0;}
for(int i=1; i<extension; i++){
if(bb.get(i)!=bases[loc+i]){
return 0;
}
}
leftReplacement=bb.get(0);
}
{
bb.clear();
final long rkmer=kmers.get(d);
final long kmer=rcomp(rkmer);
int extension=extendToRight2(bb, null, rightBuffer, errorExtension, true, kmer, rkmer);
if(extension<errorExtension){return 0;}
bb.reverseComplementInPlace();
for(int i=0; i<extension-1; i++){
if(bb.get(i)!=bases[loc+i+1-extension]){
return 0;
}
}
rightReplacement=bb.get(extension-1);
}
if(leftReplacement!=rightReplacement){return 0;}
if(bases[loc]==leftReplacement){return 0;}
if(!isSimilar(a, leftReplacement, kmers, counts)){return 0;}
bases[loc]=leftReplacement;
assert(d==a+k+1);
tables.regenerateKmers(bases, kmers, counts, a);
return 1;
}
private int correctSingleBaseRight(final int a, final byte[] bases, final byte[] quals, final int[] leftBuffer, final int[] rightBuffer,
final LongList kmers, final IntList counts, final ByteBuilder bb, final int errorExtension0){
final byte leftReplacement;
final int loc=a+k;
final int errorExtension=Tools.min(errorExtension0, bases.length-loc);
{
bb.clear();
final long kmer=kmers.get(a);
final long rkmer=rcomp(kmer);
int extension=extendToRight2(bb, null, rightBuffer, errorExtension, true, kmer, rkmer);
if(extension<errorExtension){return 0;}
for(int i=1; i<extension; i++){
if(bb.get(i)!=bases[loc+i]){
return 0;
}
}
leftReplacement=bb.get(0);
}
if(bases[loc]==leftReplacement){return 0;}
if(!isSimilar(a, leftReplacement, kmers, counts)){return 0;}
bases[loc]=leftReplacement;
tables.regenerateKmers(bases, kmers, counts, a);
return 1;
}
private boolean isSimilar(int a, byte newBase, LongList kmers, IntList counts){
long kmer=kmers.get(a);
final long x=AminoAcid.baseToNumber[newBase];
kmer=((kmer<<2)|x)&mask;
long rkmer=rcomp(kmer);
int count=getCount(kmer, rkmer);
int aCount=counts.get(a);
boolean similar=isSimilar(aCount, count);
return similar;
}
/*--------------------------------------------------------------*/
/*---------------- Inherited Abstract Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
final void makeKhist(){
tables.makeKhist(outHist, histColumns, histMax, histHeader, histZeros, true, smoothHist, gcHist, false, 0.01, 1, 1);
}
@Override
final void dumpKmersAsText(){
tables.dumpKmersAsBytes_MT(outKmers, minToDump, maxToDump, true, null);
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
@Override
public final KmerTableSet tables(){return tables;}
public final KmerTableSet tables;
/** Normal kmer length */
final int k;
/** k-1; used in some expressions */
final int k2;
final int shift;
final int shift2;
final long mask;
}
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