1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251
|
package barcode;
import java.io.File;
import java.util.ArrayList;
import java.util.LinkedHashSet;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextStreamWriter;
import shared.LineParserS4;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import shared.TrimRead;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.ListNum;
/**
* This is intended to run simultaneously on all files from a demultiplexed lane,
* labeling each read with the barcode of the input file, then printing them
* to a single output file.
* For example, all reads from input file 52866.4.475040.GAGGCCGCCA-TTATCTAGCT.filter-DNA.fastq.gz
* would have "(tab)GAGGCCGCCA+TTATCTAGCT" appended to their headers.
* @author Brian Bushnell
* @date May 10, 2024
*
*/
public class TagAndMerge {
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
TagAndMerge x=new TagAndMerge(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public TagAndMerge(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(a.equals("trim")){
trimLen=(b==null ? 1 : Integer.parseInt(b));
}else if(a.equals("r1only") || a.equals("read1only") || a.equals("dropr2")){
dropR2=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("shrinkHeader") || a.equals("shrink")){
shrinkHeader=Parse.parseBoolean(b);
}else if(a.equals("barcodes") || a.equals("barcodesout")){
barcodesOutFile=b;
}else if(a.equals("remap")){
remap=(b==null ? null : b.toCharArray());
}else if(a.equals("in")){
in.clear();
String[] b2=(b==null) ? null : (new File(b).exists() ? new String[] {b} : b.split(","));
for(String b3 : b2){in.add(b3);}
}else if(b==null && new File(arg).exists()){
in.add(arg);
}else if(parser.parse(arg, a, b)){
//do nothing
}else{
assert(false) : "Unknown parameter "+args[i];
outstream.println("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
out1=parser.out1;
}
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, null, true, overwrite, append, false);
}
void process(Timer t){
final ConcurrentReadOutputStream ros;
if(out1!=null){
final int buff=4;
for(String s : in) {
assert(!out1.equalsIgnoreCase(s)) : "Input file and output file have same name.";
}
ros=ConcurrentReadOutputStream.getStream(ffout1, null, buff, null, false);
ros.start();
}else{ros=null;}
for(String s : in) {
processInner(s, ros);
}
ReadWrite.closeStream(ros);
outstream.println("Finished processing "+in.size()+" files.");
if(barcodesOutFile!=null) {
TextStreamWriter tsw=new TextStreamWriter(barcodesOutFile, overwrite, append, false);
tsw.start();
for(String tag : barcodes) {tsw.println(tag);}
tsw.poisonAndWait();
}
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
outstream.println(Tools.readsBasesOut(readsProcessed, basesProcessed, readsOut, basesOut, 8, false));
}
void processInner(String fname, ConcurrentReadOutputStream ros) {
FileFormat ffin=FileFormat.testInput(fname, FileFormat.FASTQ, null, true, true);
String tag=Barcode.parseBarcodeFromFname(fname);
assert(tag!=null) : "Can't find barcode in filename "+fname;
if(remap!=null) {
for(int i=0; i<remap.length; i+=2) {
tag=tag.replace(remap[i], remap[i+1]);
}
}
if(tag!=null && Barcode.isBarcode(tag)) {
barcodes.add(tag);
}
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin, null);
cris.start();
}
boolean paired=cris.paired();
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin==null || ffin.samOrBam()) || (r.mate!=null)==cris.paired());
}
//ln!=null prevents a compiler potential null access warning
while(ln!=null && reads!=null && reads.size()>0){
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
processPair(r1, tag);
}
if(ros!=null){ros.add(reads, ln.id);}
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
ReadWrite.closeStream(cris);
}
private void processPair(Read r, String tag) {
readsProcessed+=r.pairCount();
basesProcessed+=r.pairLength();
if(dropR2) {r.mate=null;}
processRead(r, tag);
processRead(r.mate, tag);
readsOut+=r.pairCount();
basesOut+=r.pairLength();
}
private void processRead(Read r, String tag) {
if(r==null) {return;}
if(trimLen>=0 && trimLen<r.length()) {
final int x=TrimRead.trimToPosition(r, 0, trimLen-1, 1);
assert(x>0) : x+", "+trimLen+", "+r.length();
}
bb.clear();
if(!shrinkHeader) {
bb.append(r.id).tab().append(tag);
}else {
//LH00223:36:22JWNJLT3:8:1101:31031:1064 1:N:0:AAAGACCAGG+GAGTTTGAGA
// ihp.parse(r.id);
// final LineParser lp=ihp.lp();
lp.set(r.id);
bb.colon().colon().colon().appendTerm(lp, 3).colon().appendTerm(lp, 4).colon();
bb.space().appendTerm(lp, 7);
// bb.colon().appendTerm(lp, 8).colon().appendTerm(lp, 9).colon().appendTerm(lp, 10);
bb.tab().append(tag);
}
r.id=bb.toString();
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
private ArrayList<String> in=new ArrayList<String>();
private String out1=null;
private String barcodesOutFile=null;
private LinkedHashSet<String> barcodes=new LinkedHashSet<String>();
private int trimLen=-1;
private boolean dropR2=false;
private boolean shrinkHeader=false;
private char[] remap=new char[] {'-', '+'};
private final FileFormat ffout1;
private boolean overwrite=true;
private boolean append=false;
/*--------------------------------------------------------------*/
private long maxReads=-1;
private long readsProcessed=0, basesProcessed=0;
private long readsOut=0, basesOut=0;
private final ByteBuilder bb=new ByteBuilder();
// private final IlluminaHeaderParser2 ihp=new IlluminaHeaderParser2();
private final LineParserS4 lp=new LineParserS4(":::::: ");
/*--------------------------------------------------------------*/
private java.io.PrintStream outstream=System.err;
public static boolean verbose=false;
}
|