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package bin;
import java.util.ArrayList;
import java.util.HashMap;
import java.util.Random;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import structures.ByteBuilder;
import structures.FloatList;
import structures.IntHashSet;
import structures.LongHashSet;
/**
* @author Brian Bushnell
* @date Feb 23, 2025
*
*/
public class AllToAllVectorMaker extends BinObject {
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
AllToAllVectorMaker x=new AllToAllVectorMaker(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public AllToAllVectorMaker(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
loader=new DataLoader(outstream);
loader.netFileLarge=loader.netFileMid=loader.netFileSmall=null;
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(a.equals("seed")){
seed=Long.parseLong(b);
}else if(a.equals("rate") || a.equals("positivity")){
positiveRate=Float.parseFloat(b);
}else if(a.equals("edgefraction")){
edgeFraction=Float.parseFloat(b);
}else if(a.equals("gcdif") || a.equals("maxgcdif")){
maxGCDif=Float.parseFloat(b);
}else if(a.equals("maxkmerdif")){
maxKmerDif=Float.parseFloat(b);
}else if(a.equals("maxdepthratio")){
maxDepthRatio=Float.parseFloat(b);
}else if(a.equals("lines")){
lines=Parse.parseKMG(b);
}else if(a.equals("rolls")){
baseRolls=Integer.parseInt(b);
}else if(a.equals("kmerdif") || a.equals("outkmerdif")){
outKmerDif=b;
}else if(a.equals("kmerfraction") || a.equals("outkmerfraction")){
outKmerFraction=b;
}else if(a.equals("minlen")){
minlen=Parse.parseIntKMG(b);
}else if(a.equals("maxlen")){
maxlen=Parse.parseIntKMG(b);
}else if(a.equalsIgnoreCase("printSizeInVector")){
Oracle.printSizeInVector=Parse.parseBoolean(b);
}else if(loader.parse(arg, a, b)){
//do nothing
}else if(SimilarityMeasures.parse(arg, a, b)){
//do nothing
}else if(parser.parse(arg, a, b)){
//do nothing
}else{
// throw new RuntimeException("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
outstream.println("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
out1=parser.out1;
}
maxProduct=maxKmerDif*maxDepthRatio*0.75f;
KmerProb.load();
ffout1=FileFormat.testOutput(out1, FileFormat.TXT, null, true, true, false, false);
}
void process(Timer t){
validation=true;
ArrayList<Contig> contigs=allContigs=loader.loadData();
if(verbose){outstream.println("Finished reading data.");}
loader.trimEdges(contigs, Binner.maxEdges, Binner.minEdgeWeight, true);
HashMap<Integer, ArrayList<Contig>> map=new HashMap<Integer, ArrayList<Contig>>();
for(Contig c : contigs) {
if(c.labelTaxid>0) {
ArrayList<Contig> list=map.get(c.labelTaxid);
if(list==null) {map.put(c.labelTaxid, list=new ArrayList<Contig>());}
list.add(c);
}
}
outputResults(contigs, map);
if(outKmerDif!=null) {
outputKmerDifs(outKmerDif, 0);
outputKmerDifs(outKmerDif, 1);
}
if(outKmerFraction!=null) {
outputKmerDifFraction(outKmerFraction, 1.0/1024, 0.25);
}
t.stop();
outstream.println("Positive: \t"+positiveLines);
outstream.println("Negative: \t"+negativeLines);
outstream.println("Time: \t"+t);
outstream.println("Reads Processed: "+loader.contigsLoaded+
" \t"+Tools.format("%.2fk bases/sec", (loader.basesLoaded/(double)(t.elapsed))*1000000));
assert(!errorState) : "An error was encountered.";
}
private void outputResults(ArrayList<Contig> contigs, HashMap<Integer, ArrayList<Contig>> map){
LongHashSet used=new LongHashSet();
ByteStreamWriter bsw=ByteStreamWriter.makeBSW(ffout1);
randy=Shared.threadLocalRandom(seed);
Oracle oracle=new Oracle(999999, 0);
if(bsw!=null) {//Print header
vecBuffer.clear();
oracle.toVector(contigs.get(0), contigs.get(1), vecBuffer, true);
bsw.print("#dims\t").print(vecBuffer.size-1).tab().println(1);
}
ArrayList<ArrayList<Contig>> clusters=new ArrayList<ArrayList<Contig>>(map.values());
while(linesOut<lines) {
final boolean positive=(randy.nextFloat()<=positiveRate);
ByteBuilder bb=null;
while(bb==null) {bb=makeLine(clusters, oracle, positive);}
if(bb!=null) {
if(bsw!=null) {
bsw.print(bb.nl());
bb.clear();
}
linesOut++;
}
}
if(bsw!=null) {
errorState=bsw.poisonAndWait() | errorState;
}
}
private void outputKmerDifs(String fname, int sign) {
fname=fname.replaceFirst("%", sign+"");
ByteStreamWriter bsw=ByteStreamWriter.makeBSW(fname, true, false, true);
FloatList[] matrix=kmerDifMatrix[sign];
assert(matrix!=null);
for(int lenIdx=0; lenIdx<matrix.length; lenIdx++) {
FloatList list=matrix[lenIdx];
// System.err.println(lenIdx+": "+(list==null ? "null" : list.size()+""));
if(list!=null && list.size()>=100) {
int length=KmerProb.dequantizeLength(lenIdx);
list.sort();
bsw.print(length).tab().print(list.size());
for(int i=0; i<=100; i++) {
bsw.tab().print(list.percentile(i*0.01), 8);
}
bsw.println();
}
}
int x=Tools.binarySearch(new float[1], 1);
//TODO: use this to make an array of percentiles indexed by kmer dif.
bsw.poisonAndWait();
}
private void outputKmerDifFraction(String fname, double incr, double max) {
ByteStreamWriter bsw=ByteStreamWriter.makeBSW(fname, true, false, true);
bsw.println("#ceil(size)\tcount\tprobs");
FloatList[] matrix1=kmerDifMatrix[1];
FloatList[] matrix0=kmerDifMatrix[0];
bsw.print(" \t ");
float key=0;
for(int i=0; key<=max; i++) {
key=(float)(i*incr);
bsw.tab().print(key, 10);
}
for(int lenIdx=0; lenIdx<matrix1.length; lenIdx++) {
FloatList list1=matrix1[lenIdx];
FloatList list0=matrix0[lenIdx];
int size=Tools.min(list1==null ? 0 : list1.size(), list0==null ? 0 : list0.size());
if(size>100) {
int length=KmerProb.dequantizeLength(lenIdx);
FloatList fractions=fractionIndexedByKmerDif(list1, list0, incr, max);
bsw.print(length).tab().print(size);
for(int i=0; i<fractions.size(); i++) {
bsw.tab().print(fractions.get(i), 8);
}
bsw.println();
// bsw.poisonAndWait();
// assert(false);
}
}
int x=Tools.binarySearch(new float[1], 1);
//TODO: use this to make an array of percentiles indexed by kmer dif.
bsw.poisonAndWait();
}
private FloatList fractionIndexedByKmerDif(FloatList plus, FloatList minus, double incr, double max) {
plus.shrink().sort();
minus.shrink().sort();
float invPlus=1f/Math.max(1, plus.size());
float invMinus=1f/Math.max(1, minus.size());
FloatList fractions=new FloatList(1+(int)Math.ceil(max/incr));
float key=0;
for(int i=0; key<=max; i++) {
key=(float)(i*incr);
int idxPlus=Tools.max(1, Tools.binarySearch(plus.array, key));
int idxMinus=Tools.binarySearch(minus.array, key);
float fractionPlus=idxPlus*invPlus;
float fractionMinus=idxMinus*invMinus;
float fraction=fractionPlus/(fractionPlus+fractionMinus);
fractions.add(fraction);
}
for(int i=fractions.size()-2; i>=0; i--) {//Fix low sample size weirdness
fractions.set(i, Tools.max(fractions.get(i), fractions.get(i+1)));
}
return fractions;
}
// private ByteBuilder makeLine(ArrayList<Contig> contigs, HashMap<Integer, ArrayList<Contig>> map,
// LongHashSet used, Oracle oracle) {
// Contig a=null;
// while(a==null || a.labelTaxid<1 || a.size()<minlen) {
// int idx=randomIndex(randy, contigs.size(), baseRolls+1);
// a=contigs.get(idx);
// }
// assert(a.labelTaxid>0);
// boolean positive=(randy.nextFloat()<=positiveRate);
// ArrayList<Contig> list=(positive ? map.get(a.labelTaxid) : contigs);
// Contig b=findOther(a, list, used, null, randy, positive);
// if(b==null) {return null;}
// assert(b.labelTaxid>0) : a.name()+", "+b.name()+", "+positive;
// vecBuffer.clear();
// oracle.toVector(a, b, vecBuffer, true);
// if(outKmerDif!=null || outKmerFraction!=null) {
// int same=(a.labelTaxid==b.labelTaxid) ? 1 : 0;
// float dif=SimilarityMeasures.calculateDifferenceAverage(a.counts, b.counts);
// int size=(int)Tools.min(a.size(), b.size());
// FloatList difs=getDifList(size, same);
// difs.add(dif);
// assert(getDifList(size, same).size>0);
// }
//// assert(false) : Arrays.toString(kmerDifMatrix)+", "+
//// Arrays.toString(kmerDifMatrix[0])+", "+Arrays.toString(kmerDifMatrix[1]);
//
// return toLine(vecBuffer);
// }
private ByteBuilder makeLine(ArrayList<ArrayList<Contig>> clusters, Oracle oracle, final boolean positive) {
ArrayList<Contig> alist=clusters.get(randy.nextInt(clusters.size()));
ArrayList<Contig> blist=alist;
while(!positive && alist==blist) {blist=clusters.get(randy.nextInt(clusters.size()));}
FloatList vector=null;
for(int i=0; i<9 && vector==null; i++) {
vector=makeVector(alist, blist, minlen, maxlen, oracle);
}
// System.err.println(vector==null ? "fail" : "success");
return vector==null ? null : toLine(vector);
}
private FloatList makeVector(ArrayList<Contig> alist, ArrayList<Contig> blist,
int minSize, int maxSize, Oracle oracle) {
int numClusters=randy.nextInt(3);
// System.err.println(numClusters+", "+(alist==blist));
// System.err.println("numClusters="+numClusters);
if(numClusters==0) {
IntHashSet used=new IntHashSet(7);
Contig a=selectContig(alist, minSize, maxSize, used);
Contig b=selectContig(blist, minSize, Integer.MAX_VALUE, used);
if(!passesFilter(a, b)) {return null;}
assert(a!=b);
vecBuffer.clear();
// System.err.println("size="+a.size()+", "+a.numContigs()+", "+b.size()+", "+b.numContigs());
return oracle.toVector(a, b, vecBuffer, true);
}else if(numClusters==1) {
IntHashSet used=new IntHashSet(7);
Contig a=selectContig(alist, minSize, maxSize, used);
if(a==null) {return null;}
Cluster b=selectCluster(blist, 2+randy.nextInt(maxClusterContigs-1), minSize, Integer.MAX_VALUE, used);
if(!passesFilter(a, b)) {return null;}
vecBuffer.clear();
// System.err.println("size="+a.size()+", "+a.numContigs()+", "+b.size()+", "+b.numContigs());
FloatList fl=oracle.toVector(a, b, vecBuffer, true);
decluster(b);
return fl;
}else {
Cluster a=selectCluster(alist, 2+randy.nextInt(maxClusterContigs-1), minSize, maxSize, null);
if(a==null) {return null;}
Cluster b=selectCluster(blist, 2+randy.nextInt(maxClusterContigs-1), minSize, Integer.MAX_VALUE, a.contigSet);
if(!passesFilter(a, b)) {
decluster(a);
return null;
}
vecBuffer.clear();
// System.err.println("size="+a.size()+", "+a.numContigs()+", "+b.size()+", "+b.numContigs());
FloatList fl=oracle.toVector(a, b, vecBuffer, true);
decluster(a);
decluster(b);
return fl;
}
}
private void decluster(Cluster clust) {
for(Contig c : clust) {c.cluster=null; c.dest=-1;}
clust.clear();
}
private Bin selectBin(ArrayList<Contig> list, int maxElements, int minSize, int maxSize, IntHashSet used) {
if(maxElements==1) {return selectContig(list, minSize, maxSize, used);}
return selectCluster(list, maxElements, minSize, maxSize, used);
}
private Cluster selectCluster(ArrayList<Contig> list, int maxElements, int minSize, int maxSize, IntHashSet used) {
IntHashSet set=new IntHashSet(7);
long size=0;
for(int i=0; i<100; i++) {
Contig c=list.get(randy.nextInt(list.size()));
long size2=size+c.size();
if(size2>=minSize && size2<=maxSize && !set.contains(c.id())
&& (used==null || !used.contains(c.id()))) {
set.add(c.id());
size=size2;
}
if(set.size()>=maxElements) {break;}
if(size>minSize) {
if(i>20 && set.size()>=2) {break;}
if(randy.nextFloat()<0.05f) {break;}
}
}
if(size<minSize || size>maxSize) {return null;}//fail
Cluster clust=new Cluster(0);
for(int i : set.toArray()) {
clust.add(allContigs.get(i));
}
return clust;
}
private Contig selectContig(ArrayList<Contig> list, int minSize, int maxSize, IntHashSet used) {
for(int i=0; i<40; i++) {
Contig c=list.get(randy.nextInt(list.size()));
if(c.size()>=minSize && c.size()<=maxSize && (used==null || !used.contains(c.id()))) {
used.add(c.id());
return c;
}
}
// System.err.println("Can't find contig in range ("+minSize+", "+maxSize+") in list: ");
// for(int i=0; i<list.size() && i<1000; i++) {
// System.err.print(list.get(i).size()+", ");
// }
return null;
}
private ByteBuilder toLine(FloatList vector) {
lineBuffer.clear();
for(int i=0; i<vector.size(); i++) {
if(i>0) {lineBuffer.tab();}
lineBuffer.append(vector.get(i), 7, true);
}
if(vector.lastElement()==1) {positiveLines++;}
else {negativeLines++;}
return lineBuffer;
}
// private Contig findOther(final Contig a, ArrayList<Contig> contigs,
// LongHashSet used, Oracle oracle, Random randy, boolean positive) {
// for(int i=0; i<100; i++) {
// int idx=randomIndex(randy, contigs.size(), baseRolls);
// Contig b=contigs.get(idx);
// if(a.pairMap!=null && randy.nextFloat()<edgeFraction) {
// ArrayList<KeyValue> edges=KeyValue.toList(a.pairMap);
// KeyValue kv=edges.get(randy.nextInt(Tools.min(edges.size(), 4)));
// if(kv.key<allContigs.size()) {b=allContigs.get(kv.key);}
// positive=(a.labelTaxid==b.labelTaxid);//Keep it either way
//// System.err.print('.');
// }
// boolean same=(a.labelTaxid==b.labelTaxid);
// if(a!=b && b.labelTaxid>0 && (same || Math.abs(a.gc()-b.gc())<=maxGCDif) &&
// (a.size()<=maxlen && b.size()<=maxlen) && b.size()>=minlen) {
// final long key=toKey(a.id(), b.id());
// if((a.labelTaxid==b.labelTaxid)==positive && !used.contains(key)) {
// if(same || oracle==null || oracle.similarity(a, b, 1)>=0) {
// used.add(key);
// return b;
// }
// }
// }
// }
// return null;
// }
private boolean passesFilter(Bin a, Bin b) {
if(a==null || b==null || a==b) {return false;}
final float gcDif=Tools.absdif(a.gc(), b.gc());
final boolean same=a.labelTaxid==b.labelTaxid;
if(gcDif>maxGCDif) {
// System.err.println("Failed filter: "+same+", gcDif="+gcDif);
return false;
}
final float depthRatio=a.depthRatio(b);
if(depthRatio>maxDepthRatio) {
// System.err.println("Failed filter: "+same+", depthRatio="+depthRatio);
return false;
}
final float kmerDif=SimilarityMeasures.calculateDifferenceAverage(a.tetramers, b.tetramers);
if(kmerDif>maxKmerDif) {
// System.err.println("Failed filter: "+same+", kmerDif="+kmerDif);
return false;
}
final float product=kmerDif*depthRatio;
if(product>maxProduct) {
// System.err.println("Failed filter: "+same+", product="+product);
return false;
}
return true;
}
private int randomIndex(Random randy, int max, int rolls) {
int idx=randy.nextInt(max);
for(int i=randy.nextInt(rolls+1); i>0; i--) {
idx=Math.min(idx, randy.nextInt(max));
}
return idx;
}
private static long toKey(int a, int b) {
return (((long)Math.min(a, b))<<32)|(long)Math.max(a, b);
}
/*--------------------------------------------------------------*/
FloatList getDifList(int size, int sameGenome) {
int idx=KmerProb.quantizeLength(size);
FloatList[] matrix=kmerDifMatrix[sameGenome];
if(matrix[idx]==null) {matrix[idx]=new FloatList();}
return matrix[idx];
}
FloatList[][] kmerDifMatrix=new FloatList[2][38];
/*--------------------------------------------------------------*/
private String out1=null;
private String outKmerDif=null;
private String outKmerFraction=null;
private final FileFormat ffout1;
DataLoader loader=null;
long seed=-1;
long lines=1000000;
long linesOut=0;
long posCount=0;
long negCount=0;
float positiveRate=0.5f;
float edgeFraction=0.1f;
int baseRolls=2;
long positiveLines=0;
long negativeLines=0;
int maxClusterContigs=9;
Random randy;
float maxGCDif=1.0f;//0.15
float maxDepthRatio=1000.0f;//2.4
float maxKmerDif=1.0f;//0.02
final float maxProduct;
int minlen=100;
int maxlen=2000000000;
ArrayList<Contig> allContigs=null;
ArrayList<ArrayList<Contig>> allSets=null;
private final ByteBuilder lineBuffer=new ByteBuilder();
private final FloatList vecBuffer=new FloatList();
/*--------------------------------------------------------------*/
private boolean errorState=false;
/*--------------------------------------------------------------*/
private java.io.PrintStream outstream=System.err;
public static boolean verbose=false;
}
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