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package bin;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.HashMap;
import java.util.concurrent.locks.ReadWriteLock;
import java.util.concurrent.locks.ReentrantReadWriteLock;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.LineParser1;
import shared.LineParser4;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FastaReadInputStream;
import stream.Read;
import stream.SamLine;
import stream.SamLineStreamer;
import stream.SamReadInputStream;
import stream.SamStreamer;
import structures.ByteBuilder;
import structures.FloatList;
import structures.IntList;
import structures.IntLongHashMap;
import structures.ListNum;
import tax.TaxTree;
import template.Accumulator;
import template.ThreadWaiter;
import tracker.ReadStats;
import var2.SamFilter;
/**
* Renames contigs based on a sam file.
* Appends coverage (cov_#).
* Also appends taxid (taxid_#) if reads have taxids,
* and the contig does not already have a taxid.
*
* @author Brian Bushnell
* @date September 6, 2019
*
*/
public class ContigRenamer implements Accumulator<ContigRenamer.ProcessThread> {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
ContigRenamer x=new ContigRenamer(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public ContigRenamer(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
samFilter.includeUnmapped=false;
samFilter.includeSupplimentary=false;
samFilter.includeDuplicate=false;
samFilter.includeNonPrimary=false;
samFilter.includeQfail=false;
// samFilter.minMapq=4;
{//Parse the arguments
final Parser parser=parse(args);
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
extin=parser.extin;
out=parser.out1;
extout=parser.extout;
}
{
samFilter.setSamtoolsFilter();
streamerThreads=Tools.max(1, Tools.min(streamerThreads, Shared.threads()));
assert(streamerThreads>0) : streamerThreads;
}
validateParams();
fixExtensions(); //Add or remove .gz or .bz2 as needed
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
//Create output FileFormat objects
ffout=FileFormat.testOutput(out, FileFormat.FASTA, extout, true, overwrite, append, ordered);
//Create input FileFormat objects
ffref=FileFormat.testInput(ref, FileFormat.FASTA, null, true, true);
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
//Create a parser object
Parser parser=new Parser();
//Set any necessary Parser defaults here
//parser.foo=bar;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("ref") || a.equals("contigs") || a.equals("in")){
ref=b;
}else if(a.equals("ordered")){
ordered=Parse.parseBoolean(b);
}else if(a.equals("delimiter")){
delimiter=Parse.parseSymbol(b);
}else if(a.equals("wipe")){
wipe=Parse.parseBoolean(b);
}else if(a.equals("depth") || a.equals("cov") || a.equals("adddepth")){
addDepth=Parse.parseBoolean(b);
}else if(a.equals("tid") || a.equalsIgnoreCase("taxid")){
addTid=Parse.parseBoolean(b);
}else if(a.equals("cami")){
cami=b;
}else if(a.equals("clearfilters")){
if(Parse.parseBoolean(b)){
samFilter.clear();
}
}else if(a.equals("parse_flag_goes_here")){
long fake_variable=Parse.parseKMG(b);
//Set a variable here
}else if(a.equals("sam") || a.equals("insam") || a.equals("samin")){
Tools.getFileOrFiles(b, in, false, false, true, false);
}else if(samFilter.parse(arg, a, b)){
//do nothing
}else if(new File(arg).isFile()){
in.add(arg);
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
}
}
return parser;
}
/** Add or remove .gz or .bz2 as needed */
private void fixExtensions(){
in=Tools.fixExtension(in);
ref=Tools.fixExtension(ref);
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure there is an input file
if(in==null){throw new RuntimeException("Error - an input file is required.");}
//Ensure there is an input file
if(ref==null){throw new RuntimeException("Error - a reference file is required.");}
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out)){
outstream.println((out==null)+", "+out);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output file "+out+"\n");
}
ArrayList<String> x=new ArrayList<String>();
x.addAll(in);
x.add(ref);
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, x)){
throw new RuntimeException("\nCan't read some input files.\n");
}
x.add(out);
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, x.toArray(new String[0]))){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
assert(FastaReadInputStream.settingsOK());
}
/** Ensure parameter ranges are within bounds and required parameters are set */
private boolean validateParams(){
// assert(minfoo>0 && minfoo<=maxfoo) : minfoo+", "+maxfoo;
// assert(false) : "TODO";
return true;
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Turn off read validation in the input threads to increase speed
final boolean vic=Read.VALIDATE_IN_CONSTRUCTOR;
Read.VALIDATE_IN_CONSTRUCTOR=Shared.threads()<4;
//Reset counters
readsProcessed=readsOut=0;
basesProcessed=basesOut=0;
if(in.size()>0) {
processSam();
}else if(cami!=null) {
processCami();
}else {
assert(false) : "No input.";
}
processReference();
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Reset read validation
Read.VALIDATE_IN_CONSTRUCTOR=vic;
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
outstream.println(Tools.readsBasesOut(readsProcessed, basesProcessed, readsOut, basesOut, 8, false));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
private void processSam() {
for(int i=0; i<in.size(); i++) {
FileFormat ffin=FileFormat.testInput(in.get(i), FileFormat.SAM, extin, true, true);
//Create a read input stream
SamStreamer ss=makeStreamer(ffin);
if(scafMap.isEmpty()) {scafMap=makeScafMap();}
//Process the reads in separate threads
spawnThreads(ss);
for(Scaf scaf : scafMap.values()) {
scaf.process();
scaf.map.clear();
}
}
}
private void processCami() {
LineParser1 lp=new LineParser1('\t');
ArrayList<byte[]> lines=ByteFile.toLines(cami);
scafMap=new HashMap<String, Scaf>();
addDepth=false;
addTid=true;
for(byte[] line : lines) {
if(!Tools.startsWith(line, '@')){
lp.set(line);
String name=lp.parseString(0);
int taxID=lp.parseInt(2);
Scaf scaf=new Scaf(name, 1);
Scaf old=scafMap.putIfAbsent(name, scaf);
assert(old==null) : "Name conflict for scaffold "+name;
scaf.tidList.add(taxID);
scaf.covList.add(1);
}
}
}
private ConcurrentReadInputStream makeRefCris(){
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffref, null);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
boolean paired=cris.paired();
assert(!paired) : "References should not be paired.";
return cris;
}
private SamStreamer makeStreamer(FileFormat ff){
if(ff==null){return null;}
SamStreamer ss=new SamLineStreamer(ff, streamerThreads, true, maxReads);
ss.start(); //Start the stream
if(verbose){outstream.println("Started Streamer");}
return ss;
}
private static HashMap<String, Scaf> makeScafMap() {
//@SQ SN:foo LN:999
LineParser4 lp=new LineParser4("\t:\t:");
ArrayList<byte[]> header=SamReadInputStream.getSharedHeader(true);
HashMap<String, Scaf> map=new HashMap<String, Scaf>(header.size()+1);
for(byte[] line : header) {
if(Tools.startsWith(line, "@SQ\t")){
lp.set(line);
assert(lp.termEquals("SN", 1)) : "\n"+lp.parseString(1)+"\n"+lp+"\n"+new String(line);
String name=lp.parseString(2);
assert(lp.termEquals("LN", 3));
int len=lp.parseInt(4);
Scaf scaf=new Scaf(name, len);
Scaf old=map.putIfAbsent(name, scaf);
assert(old==null) : "Name conflict for scaffold "+name;
}
}
return map;
}
private ConcurrentReadOutputStream makeCros(){
if(ffout==null){return null;}
//Select output buffer size based on whether it needs to be ordered
final int buff=(ordered ? Tools.mid(16, 128, (Shared.threads()*2)/3) : 8);
final ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(ffout, null, buff, null, false);
ros.start(); //Start the stream
return ros;
}
/*--------------------------------------------------------------*/
/*---------------- Thread Management ----------------*/
/*--------------------------------------------------------------*/
/** Spawn process threads */
private void spawnThreads(final SamStreamer ss){
//Do anything necessary prior to processing
//Determine how many threads may be used
final int threads=Shared.threads();
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(ss, i));
}
//Start the threads
for(ProcessThread pt : alpt){
pt.start();
}
//Wait for threads to finish
boolean success=ThreadWaiter.waitForThreadsToFinish(alpt, this);
errorState&=!success;
//Do anything necessary after processing
}
@Override
public final void accumulate(ProcessThread pt){
readsProcessed+=pt.readsProcessedT;
basesProcessed+=pt.basesProcessedT;
errorState|=(!pt.success);
}
@Override
public final boolean success(){return !errorState;}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
private void processReference(){
ConcurrentReadInputStream cris=makeRefCris();
ConcurrentReadOutputStream ros=makeCros();
ListNum<Read> ln=null;
for(ln=cris.nextList(); ln!=null && ln.size()>0; ln=cris.nextList()) {
for(Read r : ln) {
r.id=(rename(r.id, r.length(), r.numericID));
readsOut++;
basesOut+=r.length();
readsProcessed++;
basesProcessed+=r.length();
}
if(ros!=null) {ros.add(ln.list, ln.id);}
cris.returnList(ln);
}
//Notify the input stream that the list was used
cris.returnList(ln);
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
errorState|=ReadWrite.closeStreams(cris, ros);
}
static String toShortName(String s) {
int idx=Tools.indexOfWhitespace(s);
return idx<0 ? s : s.substring(0, idx);
}
static String toShortName(byte[] s) {
int idx=Tools.indexOfWhitespace(s);
return idx<0 ? new String(s) : new String(s, 0, idx);
}
private String rename(final String old, final int scaflen, long numericID) {
final String name=old;
final String shortName=toShortName(name);
final int oldTaxid=TaxTree.parseHeaderStatic2(old, null);
Scaf scaf=scafMap.get(shortName);
if(scaf==null) {scaf=scafMap.get(name);}
assert(scaf!=null) : "Can't find "+shortName+" in map of size "+scafMap.size();
if(scaf==null) {return old+delimiter+"cov_0";}
IntList tids=scaf.tidList;
FloatList covs=scaf.covList;
float maxCov=0;
int maxTid=-1;
for(int i=0; i<covs.size(); i++) {
int tid=tids.get(i);
float cov=covs.get(i);
if(maxTid<=0 || cov>maxCov) {
maxCov=cov;
maxTid=tid;
}
}
ByteBuilder bb=new ByteBuilder(old.length()+(covs.size*9)+20);
if(wipe) {
bb.append("contig_").append(numericID);
}else {
bb.append(old);
}
if(oldTaxid<0 && maxTid>0 && addTid) {bb.append(delimiter).append("tid_").append(maxTid);}
for(int i=0; i<covs.size && addDepth; i++) {
bb.append(delimiter).append("cov_").append(covs.get(i), 2);
}
// assert(false) : oldTaxid+", "+maxTid+", "+bb;
return bb.toString();
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/** This class is static to prevent accidental writing to shared variables.
* It is safe to remove the static modifier. */
class ProcessThread extends Thread {
//Constructor
ProcessThread(final SamStreamer ss_, final int tid_){
ss=ss_;
tid=tid_;
}
//Called by start()
@Override
public void run(){
//Do anything necessary prior to processing
//Process the reads
processInner();
//Do anything necessary after processing
//Indicate successful exit status
success=true;
}
/** Iterate through the reads */
void processInner(){
//Grab and process all lists
for(ListNum<SamLine> ln=ss.nextLines(); ln!=null; ln=ss.nextLines()){
// if(verbose){outstream.println("Got list of size "+list.size());} //Disabled due to non-static access
processList(ln);
}
}
void processList(ListNum<SamLine> ln){
//Grab the actual read list from the ListNum
final ArrayList<SamLine> reads=ln.list;
//Loop through each read in the list
for(int idx=0; idx<reads.size(); idx++){
final SamLine r=reads.get(idx);
//Track the initial length for statistics
final int initialLength=r.length();
//Increment counters
readsProcessedT+=r.length();
basesProcessedT+=initialLength;
addLine(r);
}
}
private void addLine(SamLine sl) {
if(samFilter!=null && !samFilter.passesFilter(sl)){return;}
String key=sl.rnameS();
Scaf scaf=scafMap.get(key);
assert(scaf!=null) : "Can't find scaffold "+key+" in map of size "+scafMap.size();
scaf.add(sl);
}
/** Number of reads processed by this thread */
protected long readsProcessedT=0;
/** Number of bases processed by this thread */
protected long basesProcessedT=0;
/** True only if this thread has completed successfully */
boolean success=false;
/** Shared input stream */
private final SamStreamer ss;
/** Thread ID */
final int tid;
}
private static class Scaf {
Scaf(String shortName_, int length_) {
name=shortName_;
length=length_;
}
void add(SamLine sl) {
int taxid=TaxTree.parseHeaderStatic2(sl.qname, null);
int length=sl.length();
synchronized(this) {map.increment(taxid, length);}
}
void process() {
final int[] keys=map.keys();
final long[] values=map.values();
final int invalid=map.invalid();
int taxid=-1;
long totalMappedBases=0;
long maxMappedBases=0;
for(int i=0; i<keys.length; i++) {
int key=keys[i];
long value=values[i];
if(key!=invalid) {
totalMappedBases+=value;
if(value>maxMappedBases) {
maxMappedBases=value;
taxid=key;
}
}
}
assert(length>0);
final float cov=totalMappedBases/(float)length;
tidList.add(taxid);
covList.add(cov);
}
final String name;
final int length;
IntLongHashMap map=new IntLongHashMap(7);
IntList tidList=new IntList(2);
FloatList covList=new FloatList(2);
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Sam input file path */
private ArrayList<String> in=new ArrayList<String>();
/** Contig input file path */
private String ref=null;
private String cami=null;
/** Primary output file path */
private String out=null;
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
/** Number of reads retained */
protected long readsOut=0;
/** Number of bases retained */
protected long basesOut=0;
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
private String delimiter=" ";
private boolean wipe=false;
private boolean addDepth=true;
private boolean addTid=true;
/*--------------------------------------------------------------*/
/** Threads dedicated to reading the sam file */
private int streamerThreads=SamStreamer.DEFAULT_THREADS;
public final SamFilter samFilter=new SamFilter();
public HashMap<String, Scaf> scafMap=new HashMap<String, Scaf>();
@Override
public final ReadWriteLock rwlock() {return rwlock;}
private final ReadWriteLock rwlock=new ReentrantReadWriteLock();
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Secondary input file */
private final FileFormat ffref;
/** Primary output file */
private final FileFormat ffout;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
/** Reads are output in input order */
private boolean ordered=false;
}
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