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package cardinality;
import java.util.Arrays;
import java.util.Random;
import dna.AminoAcid;
import fileIO.ByteStreamWriter;
import jgi.Dedupe;
import shared.Parser;
import shared.Shared;
import shared.Tools;
import stream.Read;
import structures.LongList;
import structures.SuperLongList;
import ukmer.Kmer;
/**
* Abstract superclass for cardinality-tracking structures like LogLog.
* @author Brian Bushnell
* @date Feb 20, 2020
*
*/
public abstract class CardinalityTracker {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Factory method; creates a tracker using default settings.
* Subclass is determined by static Parser.loglogType field.
*/
public static CardinalityTracker makeTracker(){
if(trackCounts || "BBLog".equalsIgnoreCase(Parser.loglogType)){
return new BBLog();//Fastest, most accurate
}else if("LogLog".equalsIgnoreCase(Parser.loglogType)){
return new LogLog();//Least accurate
}else if("LogLog2".equalsIgnoreCase(Parser.loglogType)){
return new LogLog2();//Slowest, uses mantissa
}else if("LogLog16".equalsIgnoreCase(Parser.loglogType)){
return new LogLog16();//Uses 10-bit mantissa
}else if("LogLog8".equalsIgnoreCase(Parser.loglogType)){
return new LogLog8();//Lowest memory
}
assert(false) : "TODO: "+Parser.loglogType;
throw new RuntimeException(Parser.loglogType);
}
/**
* Factory method; creates a tracker using parsed settings.
* Subclass is determined by static Parser.loglogType field.
*/
public static CardinalityTracker makeTracker(Parser p){
if(trackCounts || "BBLog".equalsIgnoreCase(Parser.loglogType)){
return new BBLog(p);
}else if("LogLog".equalsIgnoreCase(Parser.loglogType)){
return new LogLog(p);
}else if("LogLog2".equalsIgnoreCase(Parser.loglogType)){
return new LogLog2(p);
}else if("LogLog16".equalsIgnoreCase(Parser.loglogType)){
return new LogLog16(p);
}else if("LogLog8".equalsIgnoreCase(Parser.loglogType)){
return new LogLog8(p);
}
assert(false) : "TODO: "+Parser.loglogType;
throw new RuntimeException(Parser.loglogType);
}
/**
* Factory method; creates a tracker using specified settings.
* Subclass is determined by static Parser.loglogType field.
*/
public static CardinalityTracker makeTracker(int buckets_, int k_, long seed, float minProb_){
if(trackCounts || "BBLog".equalsIgnoreCase(Parser.loglogType)){
return new BBLog(buckets_, k_, seed, minProb_);
}else if("LogLog".equalsIgnoreCase(Parser.loglogType)){
return new LogLog(buckets_, k_, seed, minProb_);
}else if("LogLog2".equalsIgnoreCase(Parser.loglogType)){
return new LogLog2(buckets_, k_, seed, minProb_);
}else if("LogLog16".equalsIgnoreCase(Parser.loglogType)){
return new LogLog16(buckets_, k_, seed, minProb_);
}else if("LogLog8".equalsIgnoreCase(Parser.loglogType)){
return new LogLog8(buckets_, k_, seed, minProb_);
}
assert(false) : "TODO: "+Parser.loglogType;
throw new RuntimeException(Parser.loglogType);
}
/*--------------------------------------------------------------*/
/*---------------- Constructors ----------------*/
/*--------------------------------------------------------------*/
/** Create a tracker with parsed parameters. */
public CardinalityTracker(Parser p){
this(p.loglogbuckets, p.loglogk, p.loglogseed, p.loglogMinprob);
}
/**
* Create a tracker with specified parameters.
* @param buckets_ Number of buckets (counters)
* @param k_ Kmer length
* @param seed Random number generator seed; -1 for a random seed
* @param minProb_ Ignore kmers with under this probability of being correct
*/
public CardinalityTracker(int buckets_, int k_, long seed, float minProb_){
// if((buckets_&1)==0){buckets_=(int)Primes.primeAtLeast(buckets_);} //Legacy code, needed modulo operation
buckets=powerOf2AtLeast(buckets_);
assert(buckets>0 && Integer.bitCount(buckets)==1) : "Buckets must be a power of 2: "+buckets;
bucketMask=buckets-1;
k=Kmer.getKbig(k_);
minProb=minProb_;
//For old hash function
// tables=new long[numTables][][];
// for(int i=0; i<numTables; i++){
// tables[i]=makeCodes(steps, bits, (seed<0 ? -1 : seed+i));
// }
Random randy=Shared.threadLocalRandom(seed<0 ? -1 : seed);
hashXor=randy.nextLong();
}
/**
* Return the lowest power of 2 that is >= target.
* Provided because buckets must currently be a power of 2.
*/
public static final int powerOf2AtLeast(int target){
if(target<1){return 1;}
int ret=1, limit=Tools.min(target, 0x40000000);
while(ret<limit){ret<<=1;}
return ret;
}
/*--------------------------------------------------------------*/
/*---------------- Methods ----------------*/
/*--------------------------------------------------------------*/
/** Deprecated; use LogLogWrapper */
public static final void main(String[] args){
LogLogWrapper llw=new LogLogWrapper(args);
final boolean vic=Read.VALIDATE_IN_CONSTRUCTOR;
Read.VALIDATE_IN_CONSTRUCTOR=Shared.threads()<4;
llw.process();
Read.VALIDATE_IN_CONSTRUCTOR=vic;
}
/**
* Old table-based hash function.
* Slower than the new function.
*/
public final long hash(final long value0, final long[][] table){
long value=value0, code=0;
long mask=(bits>63 ? -1L : ~((-1L)<<bits));
for(int i=0; i<steps; i++){//I could also do while value!=0
int x=(int)(value&mask);
value>>=bits;
code=code^table[i][x];
}
return code;
}
/** Hash and add a number to this tracker */
public final void add(long number){
hashAndStore(number);
}
/**
* Hash and track the Read
* */
public final void hash(Read r){
if(r==null){return;}
if(r.length()>=k){hash(r.bases, r.quality);}
if(r.mateLength()>=k){hash(r.mate.bases, r.mate.quality);}
}
/**
* Hash and track the sequence
* */
public final void hash(byte[] bases, byte[] quals){
if(k<32){hashSmall(bases, quals);}
else{hashBig(bases, quals);}
}
/**
* Hash and track the sequence using short kmers 0<k<32
* */
public final void hashSmall(byte[] bases, byte[] quals){
final int shift=2*k;
final int shift2=shift-2;
final long mask=(shift>63 ? -1L : ~((-1L)<<shift));
int len=0;
long kmer=0, rkmer=0;
if(minProb>0 && quals!=null){//Debranched loop
assert(quals.length==bases.length) : quals.length+", "+bases.length;
float prob=1;
for(int i=0; i<bases.length; i++){
byte b=bases[i];
long x=AminoAcid.baseToNumber[b];
long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
{//Update probability
byte q=quals[i];
prob=prob*PROB_CORRECT[q];
if(len>k){
byte oldq=quals[i-k];
prob=prob*PROB_CORRECT_INVERSE[oldq];
}
}
if(x>=0){
len++;
}else{
len=0;
kmer=rkmer=0;
prob=1;
}
if(len>=k && prob>=minProb){
add(Tools.max(kmer, rkmer));
}
}
}else{
for(int i=0; i<bases.length; i++){
byte b=bases[i];
long x=AminoAcid.baseToNumber[b];
long x2=AminoAcid.baseToComplementNumber[b];
kmer=((kmer<<2)|x)&mask;
rkmer=((rkmer>>>2)|(x2<<shift2))&mask;
if(x>=0){
len++;
}else{
len=0;
kmer=rkmer=0;
}
if(len>=k){
add(Tools.max(kmer, rkmer));
}
}
}
}
/**
* Hash and track the sequence using long kmers >31
* */
public final void hashBig(byte[] bases, byte[] quals){
Kmer kmer=getLocalKmer();
int len=0;
float prob=1;
for(int i=0; i<bases.length; i++){
byte b=bases[i];
long x=Dedupe.baseToNumber[b];
kmer.addRightNumeric(x);
if(minProb>0 && quals!=null){//Update probability
prob=prob*PROB_CORRECT[quals[i]];
if(len>k){
byte oldq=quals[i-k];
prob=prob*PROB_CORRECT_INVERSE[oldq];
}
}
if(AminoAcid.isFullyDefined(b)){
len++;
}else{
len=0;
prob=1;
}
if(len>=k && prob>=minProb){
add(kmer.xor());
}
}
}
/**
* Make a table of random bitmasks for hashing.
* Superceded by new hash method.
* */
private static final long[][] makeCodes(int length, int bits, long seed){
if(true) {return null;}//Short circuit
Random randy=Shared.threadLocalRandom(seed);
int modes=1<<bits;
long[][] r=new long[length][modes];
for(int i=0; i<length; i++){
for(int j=0; j<modes; j++){
long x=randy.nextLong();
while(Long.bitCount(x)>33){
x&=(~(1L<<randy.nextInt(64)));
}
while(Long.bitCount(x)<31){
x|=(1L<<randy.nextInt(64));
}
r[i][j]=x;
}
}
return r;
}
public final float compensationFactorBuckets(){
assert(Integer.bitCount(buckets)==1) : buckets;
int zeros=Integer.numberOfTrailingZeros(buckets);
return compensationFactorLogBuckets(zeros);
}
/**
* Multiplier to compensate for overestimating genome size
* when the number of buckets is too small,
* as a function of log2(buckets)
* @param logBuckets
* @return Multiplier for final estimate
*/
public final float compensationFactorLogBuckets(int logBuckets){
float[] array=compensationFactorLogBucketsArray();
return (array!=null && logBuckets<array.length) ? array[logBuckets] : 1/(1+(1<<logBuckets));
}
public SuperLongList toFrequency(){
SuperLongList list=new SuperLongList(1000);
int[] counts=getCounts();
for(int x : counts){
if(x>0){list.add(x);}
}
list.sort();
return list;
}
/**
* Print a kmer frequency histogram.
* @param path File to which to print
* @param overwrite
* @param append
* @param supersample Adjust counts for the effect of subsampling
*/
public void printKhist(String path, boolean overwrite, boolean append, boolean supersample, int decimals){
SuperLongList sll=toFrequency();
ByteStreamWriter bsw=new ByteStreamWriter(path, overwrite, append, false);
bsw.start();
bsw.print("#Depth\tCount\n");
final double mult=Tools.max(1.0, (supersample ? cardinality()/(double)buckets : 1));
final long[] array=sll.array();
final LongList list=sll.list();
for(int depth=0; depth<array.length; depth++){
long count=array[depth];
if(count>0){
bsw.print(depth).tab();
if(supersample){
if(decimals>0){
bsw.print(count*mult, decimals).nl();
}else{
bsw.print(Tools.max(1, Math.round(count*mult))).nl();
}
}else{
bsw.print(count).nl();
}
}
}
int count=0;
long prevDepth=-1;
for(int i=0; i<list.size; i++){
long depth=list.get(i);
if(depth!=prevDepth && count>0){
assert(depth>prevDepth);
bsw.print(prevDepth).tab();
if(supersample){
if(decimals>0){
bsw.print(count*mult, decimals).nl();
}else{
bsw.print(Tools.max(1, Math.round(count*mult))).nl();
}
}else{
bsw.print(count).nl();
}
count=0;
}else{
count++;
}
prevDepth=depth;
}
if(count>0){
bsw.print(prevDepth).tab();
if(supersample){
if(decimals>0){
bsw.print(count*mult, decimals).nl();
}else{
bsw.print(Tools.max(1, Math.round(count*mult))).nl();
}
}else{
bsw.print(count).nl();
}
}
bsw.poisonAndWait();
}
/** Sum of counts array, if present */
public final long countSum(){
int[] counts=getCounts();
return counts==null ? 0 : shared.Vector.sum(counts);
}
/*--------------------------------------------------------------*/
/*---------------- Abstract Methods ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Abstract Methods ----------------*/
/*--------------------------------------------------------------*/
/** Calculate cardinality estimate from this tracker */
public abstract long cardinality();
/** Counts array, if present.
* Should be overridden for classes that track counts. */
public int[] getCounts(){
return null;
}
/** Add another tracker to this one */
public abstract void add(CardinalityTracker log);
/** Generate a 64-bit hashcode from a number, and add it to this tracker */
public abstract void hashAndStore(final long number);
/** TODO: Deprecate?
* Designed to compensate for overestimate with small numbers of buckets.
* Appears to be handled by using the harmonic mean in the case of multiple trials. */
public abstract float[] compensationFactorLogBucketsArray();
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Kmer length */
public final int k;
/** Ignore kmers under this probability of correctness */
public final float minProb;
/**
* Number of buckets for tracking.
* Must be a power of 2.
* Larger is slower and uses more memory, but more accurate.
*/
public final int buckets;
/** Mask for lower bits of hashcode to yield bucket; bucketMask==buckets-1. */
final int bucketMask;
/** Reusable kmer for hashing. */
private final ThreadLocal<Kmer> localKmer=new ThreadLocal<Kmer>();
/** The hash function will yield a different mapping for each mask here. */
private final long hashXor;
/** For long kmer mode */
protected Kmer getLocalKmer(){
Kmer kmer=localKmer.get();
if(kmer==null){
localKmer.set(new Kmer(k));
kmer=localKmer.get();
}
kmer.clearFast();
return kmer;
}
/*--------------------------------------------------------------*/
/*---------------- Deprecated Table Fields ----------------*/
/*--------------------------------------------------------------*/
static final int numTables=4;
static final int numTablesMask=numTables-1;
/** Bits hashed per cycle; no longer needed */
private static final int bits=8;
private static final int steps=(63+bits)/bits;;
// final long[][][] tables;
/*--------------------------------------------------------------*/
/*---------------- Statics ----------------*/
/*--------------------------------------------------------------*/
/** Converts quality score to probability of correctness */
public static final float[] PROB_CORRECT=Arrays.copyOf(align2.QualityTools.PROB_CORRECT, 128);
/** Inverse of PROB_CORRECT */
public static final float[] PROB_CORRECT_INVERSE=Arrays.copyOf(align2.QualityTools.PROB_CORRECT_INVERSE, 128);
/** Atomic mode allows less memory when multithreaded, but lower peak concurrency due to contention. */
public static final boolean atomic=false;//non-atomic is faster.
/** Track number of times the value in each bucket was seen. */
public static boolean trackCounts=false;
/** Ignores kmers such that x%SKIPMOD!=0, to skip expensive hash and store functions */
static final long SKIPMOD=1;//No longer used; requires a modulo operation
/** Records the last cardinality tracked, for use in static contexts (RQCFilter) */
public static long lastCardinality=-1;
// /** Ignore hashed values above this, to skip expensive read and store functions. */
// static final long maxHashedValue=((-1L)>>>3);//No longer used
/** These determine how to combine multiple buckets to yield a value.
* Mean is best even though mwa is pretty close. Median is much worse. */
public static boolean USE_MEAN=true;//Arithmetic mean
public static boolean USE_MEDIAN=false;
public static boolean USE_MWA=false;//Median-weighted-average
public static boolean USE_HMEAN=false;//Harmonic mean
public static boolean USE_GMEAN=false;//Geometric mean
}
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