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package clump;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Random;
import assemble.AbstractRemoveThread;
import dna.AminoAcid;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import jgi.BBMerge;
import kmer.KmerTableSet;
import shared.KillSwitch;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* Reduces reads to their feature kmer.
* @author Brian Bushnell
* @date Nov 10, 2015
*
*/
public class KmerReduce {
/*--------------------------------------------------------------*/
/*---------------- Static Methods ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
final boolean pigz=ReadWrite.USE_PIGZ, unpigz=ReadWrite.USE_UNPIGZ;
Timer t=new Timer();
KmerReduce x=new KmerReduce(args);
x.process(t);
ReadWrite.USE_PIGZ=pigz;
ReadWrite.USE_UNPIGZ=unpigz;
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* @param fname0 Input filename of reads
* @param k Kmer length
* @param cutoff Minimum count to retain
* @return Set of pivot kmers
*/
public static KmerTableSet getValidKmersFromReads(final String fname0, int k, int cutoff){
final String fname=fname0+"_"+(new Random().nextLong()>>>1)+".fa.gz";
assert(!new File(fname).exists());
ArrayList<String> arglist=new ArrayList<String>();
arglist.add("in="+fname0);
arglist.add("out="+fname);
arglist.add("k="+k);
String[] args=arglist.toArray(new String[0]);
main(args);
assert(false) : fname+", "+k+", "+cutoff;
KmerTableSet set=getValidKmers(fname, k, cutoff);
File f=new File(fname);
if(f.exists()){f.delete();}
return set;
}
/**
* @param fname Input filename of pivot kmers
* @param k Kmer length
* @param cutoff Minimum count to retain
* @return Set of pivot kmers
*/
public static KmerTableSet getValidKmers(final String fname, int k, int cutoff){
ArrayList<String> arglist=new ArrayList<String>();
arglist.add("in="+fname);
arglist.add("k="+k);
if(cutoff>1 && prefilter){
arglist.add("prefilter="+(cutoff-1));
}
String[] args=arglist.toArray(new String[0]);
KmerTableSet set=new KmerTableSet(args, 12);
Timer t=new Timer();
set.process(t);
// errorState|=set.errorState;
assert(!set.errorState);
t.stop();
set.prefilterArray=null;
AbstractRemoveThread.process(Shared.threads(), cutoff, Integer.MAX_VALUE, set, true);
return set;
}
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Constructor.
* @param args Command line arguments
*/
public KmerReduce(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){
//do nothing
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(a.equals("k")){
k=Integer.parseInt(b);
assert(k>0 && k<32);
}else if(a.equals("comparisons") || a.equals("c")){
//do nothing
}else if(a.equals("ecco")){
ecco=Parse.parseBoolean(b);
}else if(a.equals("rename") || a.equals("addname")){
//do nothing
}else if(a.equals("rcomp") || a.equals("reversecomplement")){
//do nothing
}else if(a.equals("condense") || a.equals("consensus")){
//do nothing
}else if(a.equals("correct") || a.equals("ecc")){
//do nothing
}else if(a.equals("groups") || a.equals("g") || a.equals("sets") || a.equals("ways")){
//do nothing
}else if(a.equals("seed")){
KmerComparator.defaultSeed=Long.parseLong(b);
}else if(a.equals("hashes")){
KmerComparator.setHashes(Integer.parseInt(b));
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
in1=parser.in1;
in2=parser.in2;
out1=parser.out1;
extin=parser.extin;
extout=parser.extout;
}
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){outstream.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+"\n");
}
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2, null, null);
cris.start();
if(verbose){outstream.println("Started cris");}
}
boolean paired=cris.paired();
final ConcurrentReadOutputStream ros;
if(out1!=null){
final int buff=Tools.max(4, Shared.threads());
assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
ros=ConcurrentReadOutputStream.getStream(ffout1, null, buff, null, false);
ros.start();
}else{ros=null;}
readsProcessed=0;
basesProcessed=0;
//Process the read stream
processInner(cris, ros);
if(verbose){outstream.println("Finished; closing streams.");}
errorState|=ReadStats.writeAll();
errorState|=ReadWrite.closeStreams(cris, ros);
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
if(errorState){
Clumpify.sharedErrorState=true;
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/** Manage threads */
public void processInner(final ConcurrentReadInputStream cris, final ConcurrentReadOutputStream ros){
if(verbose){outstream.println("Making comparator.");}
KmerComparator kc=new KmerComparator(k, false, false);
if(verbose){outstream.println("Making hash threads.");}
final int threads=Shared.threads();
ArrayList<HashThread> alht=new ArrayList<HashThread>(threads);
for(int i=0; i<threads; i++){alht.add(new HashThread(cris, ros, kc));}
if(verbose){outstream.println("Starting threads.");}
for(HashThread ht : alht){ht.start();}
if(verbose){outstream.println("Waiting for threads.");}
/* Wait for threads to die */
for(HashThread ht : alht){
/* Wait for a thread to die */
while(ht.getState()!=Thread.State.TERMINATED){
try {
ht.join();
} catch (InterruptedException e) {
e.printStackTrace();
}
}
readsProcessed+=ht.readsProcessedT;
basesProcessed+=ht.basesProcessedT;
}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
private class HashThread extends Thread{
HashThread(ConcurrentReadInputStream cris_, ConcurrentReadOutputStream ros_, KmerComparator kc_){
cris=cris_;
ros=ros_;
kc=kc_;
}
@Override
public void run(){
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
ArrayList<Read> out=new ArrayList<Read>(reads.size());
for(Read r : reads){
if(ecco && r.mate!=null){
if(r.mate!=null){BBMerge.findOverlapStrict(r, r.mate, true);}
}
final long kmer=kc.hash(r, null, 0, false);
readsProcessedT++;
basesProcessedT+=r.length();
if(kmer>=0){
Read temp=new Read(toBytes(kmer), null, header, r.numericID);
out.add(temp);
}
}
if(ros!=null){ros.add(out, ln.id);}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
final ConcurrentReadInputStream cris;
final ConcurrentReadOutputStream ros;
final KmerComparator kc;
protected long readsProcessedT=0;
protected long basesProcessedT=0;
private static final String header="1";
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
public byte[] toBytes(final long kmer){
byte[] dest=KillSwitch.allocByte1D(k);
fill(kmer, dest, 0);
return dest;
}
public void fill(final long kmer, final byte[] dest, int pos){
for(int i=k-1; i>=0; i--, pos++){
int x=(int)((kmer>>(2*i))&3);
dest[pos]=AminoAcid.numberToBase[x];
}
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
private int k=31;
static boolean prefilter=true;
/*--------------------------------------------------------------*/
/*---------------- I/O Fields ----------------*/
/*--------------------------------------------------------------*/
private String in1=null;
private String in2=null;
private String out1=null;
private String extin=null;
private String extout=null;
/*--------------------------------------------------------------*/
protected long readsProcessed=0;
protected long basesProcessed=0;
private long maxReads=-1;
protected boolean ecco=false;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
private final FileFormat ffin1;
private final FileFormat ffin2;
private final FileFormat ffout1;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
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