1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305
|
package cluster;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import dna.Data;
import fileIO.ByteFile;
import fileIO.ByteFile1;
import fileIO.ByteFile2;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextFile;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentGenericReadInputStream;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;
/**
* Appears to replace read headers with those in a text file.
* @author Brian Bushnell
* @date Feb 7, 2014
*
*/
public class MergeReadHeaders {
public static void main(String[] args){
Timer t=new Timer();
MergeReadHeaders x=new MergeReadHeaders(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public MergeReadHeaders(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Parser parser=new Parser();
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(parser.parseInterleaved(arg, a, b)){
//do nothing
}else if(a.equals("passes")){
assert(false) : "'passes' is disabled.";
// passes=Integer.parseInt(b);
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ByteFile1.verbose=verbose;
ByteFile2.verbose=verbose;
stream.FastaReadInputStream.verbose=verbose;
ConcurrentGenericReadInputStream.verbose=verbose;
stream.FastqReadInputStream.verbose=verbose;
ReadWrite.verbose=verbose;
}else if(a.equals("reads") || a.equals("maxreads")){
maxReads=Parse.parseKMG(b);
}else if(a.equals("t") || a.equals("threads")){
Shared.setThreads(b);
}else if(a.equals("build") || a.equals("genome")){
Data.setGenome(Integer.parseInt(b));
}else if(a.equals("header")){
headerFile=b;
}else if(a.equals("in") || a.equals("input") || a.equals("in1") || a.equals("input1")){
in1=b;
}else if(a.equals("in2") || a.equals("input2")){
in2=b;
}else if(a.equals("out") || a.equals("output") || a.equals("out1") || a.equals("output1")){
out1=b;
}else if(a.equals("out2") || a.equals("output2")){
out2=b;
}else if(a.equals("extin")){
extin=b;
}else if(a.equals("extout")){
extout=b;
}else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
}else if(in1==null && i==0 && Tools.looksLikeInputStream(arg)){
in1=arg;
}else if(out1==null && i==1 && !arg.contains("=")){
out1=arg;
}else{
System.err.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){System.err.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
if(in1==null || headerFile==null){throw new RuntimeException("Error - at least one input file and a header file are required.");}
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
// if(ReadWrite.isCompressed(in1)){ByteFile.FORCE_MODE_BF2=true;}
ByteFile.FORCE_MODE_BF2=true;
}
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
if(!parser.setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
}
}
}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(out2!=null && out2.equalsIgnoreCase("null")){out2=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
Parser.processQuality();
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
ffheader=FileFormat.testInput(headerFile, FileFormat.TEXT, null, true, true);
}
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2, null, null);
if(verbose){System.err.println("Started cris");}
cris.start();
}
boolean paired=cris.paired();
if(verbose){System.err.println("Input is "+(paired ? "paired" : "unpaired"));}
ConcurrentReadOutputStream ros=null;
if(out1!=null){
final int buff=4;
if(cris.paired() && out2==null && (in1==null || !in1.contains(".sam"))){
outstream.println("Writing interleaved.");
}
assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
assert(out2==null || (!out2.equalsIgnoreCase(in1) && !out2.equalsIgnoreCase(in2))) : "out1 and out2 have same name.";
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, null, null, buff, null, false);
ros.start();
}
long readsProcessed=0;
long basesProcessed=0;
TextFile tf=new TextFile(ffheader);
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
// System.err.println("Fetched "+reads);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
final Read r2=r1.mate;
{
readsProcessed++;
basesProcessed+=r1.length();
r1.id=processHeader(tf.readLine());
}
if(r2!=null){
readsProcessed++;
basesProcessed+=r2.length();
r2.id=processHeader(tf.readLine());
}
boolean remove=false;
if(remove){reads.set(idx, null);}
}
if(ros!=null){ros.add(reads, ln.id);}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
errorState|=ReadWrite.closeStreams(cris, ros);
errorState|=tf.close();
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
if(errorState){
throw new RuntimeException("ReformatReads terminated in an error state; the output may be corrupt.");
}
}
public static String processHeader(String s){
assert(s!=null);
return s;
}
/*--------------------------------------------------------------*/
public boolean errorState=false;
private String headerFile=null;
private String in1=null;
private String in2=null;
private String out1=null;
private String out2=null;
private String extin=null;
private String extout=null;
private boolean overwrite=true;
private boolean append=false;
private long maxReads=-1;
private final FileFormat ffheader;
private final FileFormat ffin1;
private final FileFormat ffin2;
private final FileFormat ffout1;
private final FileFormat ffout2;
private PrintStream outstream=System.err;
/*--------------------------------------------------------------*/
public static boolean verbose=false;
}
|