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package consensus;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.LinkedHashMap;
import java.util.Map.Entry;
import java.util.concurrent.locks.ReadWriteLock;
import java.util.concurrent.locks.ReentrantReadWriteLock;
import fileIO.ByteFile;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import prok.ProkObject;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import sketch.SketchObject;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import stream.SamLine;
import stream.SamReadStreamer;
import stream.SamStreamer;
import structures.ByteBuilder;
import structures.ListNum;
import template.Accumulator;
import template.ThreadWaiter;
import tracker.ReadStats;
import var2.Realigner;
import var2.SamFilter;
/**
* Alters a reference to represent the consensus of aligned reads.
*
* @author Brian Bushnell
* @date September 6, 1019
*
*/
public class ConsensusMaker extends ConsensusObject implements Accumulator<ConsensusMaker.ProcessThread> {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
ConsensusMaker x=new ConsensusMaker(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public ConsensusMaker(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
FASTQ.TEST_INTERLEAVED=FASTQ.FORCE_INTERLEAVED=false;
samFilter.includeUnmapped=false;
// samFilter.includeSupplimentary=false;
// samFilter.includeDuplicate=false;
samFilter.includeNonPrimary=false;
samFilter.includeQfail=false;
// samFilter.minMapq=4;
{//Parse the arguments
final Parser parser=parse(args);
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
in=parser.in1;
extin=parser.extin;
out=parser.out1;
extout=parser.extout;
silent=Parser.silent;
}
{
// if("auto".equalsIgnoreCase(atomic)){Scaffold.setCA3A(Shared.threads()>8);}
// else{Scaffold.setCA3A(Parse.parseBoolean(atomic));}
if(ploidy<1){System.err.println("WARNING: ploidy not set; assuming ploidy=1."); ploidy=1;}
samFilter.setSamtoolsFilter();
streamerThreads=Tools.max(1, Tools.min(streamerThreads, Shared.threads()));
assert(streamerThreads>0) : streamerThreads;
}
validateParams();
fixExtensions(); //Add or remove .gz or .bz2 as needed
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
//Create output FileFormat objects
ffout=FileFormat.testOutput(out, FileFormat.FASTA, extout, true, overwrite, append, ordered);
ffmodel=FileFormat.testOutput(outModel, FileFormat.ALM, null, true, overwrite, false, ordered);
//Create input FileFormat objects
ffin=FileFormat.testInput(in, FileFormat.SAM, extin, true, true);
ffref=FileFormat.testInput(ref, FileFormat.FASTA, null, true, true);
if(inModelFile!=null){
ArrayList<BaseGraph> list=(ArrayList<BaseGraph>)ReadWrite.readObject(inModelFile, false);
inModel=list.get(0);
inModel.calcProbs();
inModel.makeWeights();
}
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
//Create a parser object
Parser parser=new Parser();
//Set any necessary Parser defaults here
//parser.foo=bar;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
BaseGraph.verbose=verbose;
}else if(a.equals("ref")){
ref=b;
}else if(a.equals("outm") || a.equals("outmodel") || a.equals("model") || a.equals("alm")){
outModel=b;
}else if(a.equals("inm") || a.equals("inmodel")){
inModelFile=b;
}else if(a.equals("hist") || a.equals("histogram")){
outHistFile=b;
}else if(a.equals("realign")){
realign=Parse.parseBoolean(b);
}else if(a.equals("printscores")){
printScores=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("useMapq")){
useMapq=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("identityCeiling") || a.equalsIgnoreCase("idceiling") || a.equalsIgnoreCase("ceiling")){
double d=Double.parseDouble(b);
if(d<=2){d=d*100;}
identityCeiling=(int)d;
invertIdentity=true;
}else if(a.equalsIgnoreCase("invertIdentity")){
invertIdentity=Parse.parseBoolean(b);
}else if(a.equals("name") || a.equals("rename") || a.equals("header")){
name=b;
}else if(a.equals("noindels")){
noIndels=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("onlyConvertNs") || a.equalsIgnoreCase("nOnly") || a.equalsIgnoreCase("onlyN")){
onlyConvertNs=Parse.parseBoolean(b);
}else if(a.equals("ploidy")){
ploidy=Integer.parseInt(b);
}else if(a.equals("mindepth")){
minDepth=Integer.parseInt(b);
}else if(a.equals("trimdepth") || a.equals("trimdepthfraction")){
trimDepthFraction=Float.parseFloat(b);
}else if(a.equalsIgnoreCase("trimNs")){
trimNs=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("mafn") || a.equals("mafnoref")){
MAF_noref=Float.parseFloat(b);
}else if(a.equalsIgnoreCase("mafsub")){
MAF_sub=Float.parseFloat(b);
}else if(a.equalsIgnoreCase("mafdel")){
MAF_del=Float.parseFloat(b);
}else if(a.equalsIgnoreCase("mafins")){
MAF_ins=Float.parseFloat(b);
}else if(a.equalsIgnoreCase("maf")){
MAF_ins=MAF_noref=MAF_del=MAF_sub=Float.parseFloat(b);
}else if(a.equalsIgnoreCase("mafindel")){
MAF_ins=MAF_del=Float.parseFloat(b);
}else if(a.equals("clearfilters")){
if(Parse.parseBoolean(b)){
samFilter.clear();
}
}else if(a.equals("parse_flag_goes_here")){
long fake_variable=Parse.parseKMG(b);
//Set a variable here
}else if(samFilter.parse(arg, a, b)){
//do nothing
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
}
}
if(ref!=null && !Tools.existsInput(ref)){
specialRef=ProkObject.isSpecialType(ref);
}
return parser;
}
/** Add or remove .gz or .bz2 as needed */
private void fixExtensions(){
in=Tools.fixExtension(in);
if(!specialRef){ref=Tools.fixExtension(ref);}
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure there is an input file
if(in==null){throw new RuntimeException("Error - an input file is required.");}
//Ensure there is an input file
if(ref==null){throw new RuntimeException("Error - a reference file is required.");}
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out, outModel, outHistFile)){
outstream.println((out==null)+", "+out);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output file "+out+" or "+outModel+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(true, true, in, inModelFile, (specialRef ? null : ref))){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in, ref, out, outModel, outHistFile, inModelFile)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
assert(FastaReadInputStream.settingsOK());
}
/** Ensure parameter ranges are within bounds and required parameters are set */
private boolean validateParams(){
// assert(minfoo>0 && minfoo<=maxfoo) : minfoo+", "+maxfoo;
return true;
}
private void writeHist(String fname){
ByteStreamWriter bsw=new ByteStreamWriter(fname, overwrite, append, false);
bsw.start();
bsw.print("#Value\tIdentity");
if(inModel!=null){
bsw.print("\tScore");
}
bsw.nl();
for(int i=0; i<idHist.length; i++){
bsw.print(0.01f*i, 2).tab().print(idHist[i]);
if(inModel!=null){
bsw.tab().print(scoreHist[i]);
}
bsw.nl();
}
errorState=bsw.poisonAndWait()|errorState;
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Turn off read validation in the input threads to increase speed
final boolean vic=Read.VALIDATE_IN_CONSTRUCTOR;
Read.VALIDATE_IN_CONSTRUCTOR=Shared.threads()<4;
//Load reference;
refMap=loadReferenceCustom();
makeRefMap2();
//Reset counters
readsProcessed=readsOut=0;
basesProcessed=basesOut=0;
alignedReads=0;
if(ffin.samOrBam()){
//Create a read input stream
final SamStreamer ss=makeStreamer(ffin);
//Process the reads in separate threads
spawnThreads(ss);
}else{
Shared.capBufferLen(40);
//Create a read input stream
final ConcurrentReadInputStream cris=makeCris(ffin);
//Process the reads in separate threads
spawnThreads(cris);
}
//Optionally create a read output stream
final ConcurrentReadOutputStream ros=makeCros();
outputConsensus(ros);
if(outHistFile!=null){
writeHist(outHistFile);
}
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Close the read streams
errorState|=ReadWrite.closeStream(ros);
//Reset read validation
Read.VALIDATE_IN_CONSTRUCTOR=vic;
//Report timing and results
t.stop();
if(!silent){
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
outstream.println(Tools.readsBasesOut(readsProcessed, basesProcessed, readsOut, basesOut, 8, false));
outstream.println();
}
outstream.println(Tools.number("Ref Count:", refCount, 8));
outstream.println(Tools.number("Sub Count:", subCount, 8));
outstream.println(Tools.number("Del Count:", delCount, 8));
outstream.println(Tools.number("Ins Count:", insCount, 8));
outstream.println(Tools.number("Avg Identity:", 100*identitySum/alignedReads, 3, 8));
if(scoreSum>0){
outstream.println(Tools.number("Avg Score:", 100*scoreSum/alignedReads, 3, 8));
}
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
private synchronized LinkedHashMap<String, BaseGraph> loadReferenceCustom(){
assert(!loadedRef);
if(specialRef){return loadReferenceSpecial();}
ConcurrentReadInputStream cris=makeRefCris();
LinkedHashMap<String, BaseGraph> map=new LinkedHashMap<String, BaseGraph>();
ListNum<Read> ln=cris.nextList();
for(; ln!=null && !ln.isEmpty(); ln=cris.nextList()){
if(verbose){outstream.println("Fetched "+ln.size()+" reference sequences.");}
for(Read r : ln){
if(r.bases!=null && r.bases.length>0){
BaseGraph bg=new BaseGraph(r.id, r.bases, r.quality, r.numericID, 0);
map.put(r.name(), bg);
// if(inModel!=null && inModel.name.equals(bg.name)){
//// assert(false) : Arrays.toString(bg.refWeights)+"\n"+Arrays.toString(inModel.refWeights);
// bg.refWeights=inModel.refWeights;
// bg.insWeights=inModel.insWeights;
// bg.delWeights=inModel.delWeights;
// }else{
// bg.insWeights=new float[bg.ref.length];
// bg.delWeights=new float[bg.ref.length];
// Arrays.fill(bg.insWeights, 1);
// Arrays.fill(bg.delWeights, 1);
//// assert(false) : Arrays.toString(bg.delWeights);
// }
}
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
if(verbose){outstream.println("Loaded "+map.size()+" reference sequences.");}
loadedRef=true;
return map;
}
//For ribo subunits in resources directory
private synchronized LinkedHashMap<String, BaseGraph> loadReferenceSpecial(){
assert(!loadedRef);
Read[] array=ProkObject.loadConsensusSequenceType(ref, false, false);
Read r=array[0];
BaseGraph bg=new BaseGraph(r.id, r.bases, r.quality, r.numericID, 0);
LinkedHashMap<String, BaseGraph> map=new LinkedHashMap<String, BaseGraph>();
map.put(r.name(), bg);
return map;
}
private synchronized void makeRefMap2(){
assert(refMap!=null && refMap2==null);
if(verbose){outstream.println("Making refMap2.");}
refMap2=new LinkedHashMap<String, BaseGraph>((refMap.size()*3)/2);
for(Entry<String, BaseGraph> e : refMap.entrySet()){
String key=e.getKey();
if(verbose){outstream.println("Considering "+key);}
BaseGraph value=e.getValue();
String key2=Tools.trimToWhitespace(key);
if(verbose){outstream.println("key2="+key2);}
// if(verbose){outstream.println("put "+key2+", "+value);}
refMap2.put(key2, value);
// if(verbose){outstream.println("putted "+key2+", "+value);}
if(defaultRname==null){defaultRname=key;}
}
// assert(false) : refMap+"\n"+refMap2;
if(verbose){outstream.println("Made refMap2.");}
}
private ConcurrentReadInputStream makeRefCris(){
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffref, null);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
boolean paired=cris.paired();
assert(!paired) : "References should not be paired.";
return cris;
}
private SamStreamer makeStreamer(FileFormat ff){
if(ff==null){return null;}
SamStreamer ss=new SamReadStreamer(ff, streamerThreads, true, maxReads);
ss.start(); //Start the stream
if(verbose){outstream.println("Started Streamer");}
return ss;
}
private ConcurrentReadInputStream makeCris(FileFormat ff){
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ff, null);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
boolean paired=cris.paired();
assert(!paired);
return cris;
}
private ConcurrentReadOutputStream makeCros(){
if(ffout==null){return null;}
//Select output buffer size based on whether it needs to be ordered
final int buff=(ordered ? Tools.mid(16, 128, (Shared.threads()*2)/3) : 8);
final ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(ffout, null, buff, null, false);
ros.start(); //Start the stream
return ros;
}
/*--------------------------------------------------------------*/
/*---------------- Thread Management ----------------*/
/*--------------------------------------------------------------*/
/** Spawn process threads */
private void spawnThreads(final SamStreamer ss){
//Do anything necessary prior to processing
//Determine how many threads may be used
final int threads=Shared.threads();
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(ss, i));
}
//Start the threads
for(ProcessThread pt : alpt){
pt.start();
}
if(verbose){outstream.println("Started worker threads.");}
//Wait for threads to finish
boolean success=ThreadWaiter.waitForThreadsToFinish(alpt, this);
errorState&=!success;
//Do anything necessary after processing
}
/** Spawn process threads */
private void spawnThreads(final ConcurrentReadInputStream cris){
//Do anything necessary prior to processing
//Determine how many threads may be used
final int threads=Shared.threads();
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(cris, i));
}
//Start the threads
for(ProcessThread pt : alpt){
pt.start();
}
if(verbose){outstream.println("Started worker threads.");}
//Wait for threads to finish
boolean success=ThreadWaiter.waitForThreadsToFinish(alpt, this);
errorState&=!success;
//Do anything necessary after processing
ReadWrite.closeStreams(cris);
}
private void outputConsensus(ConcurrentReadOutputStream ros){
if(verbose){outstream.println("Making consensus.");}
ArrayList<Read> consensusList=new ArrayList<Read>(200);
ArrayList<BaseGraph> graphList=new ArrayList<BaseGraph>(200);
long num=0;
for(Entry<String, BaseGraph> e : refMap.entrySet()){
BaseGraph bg=e.getValue();
graphList.add(bg);
Read r=bg.traverse();
if(name!=null){r.id=name;}
consensusList.add(r);
refCount+=bg.refCount;
subCount+=bg.subCount;
delCount+=bg.delCount;
insCount+=bg.insCount;
readsOut++;
basesOut+=r.length();
if(consensusList.size()>=200){
if(ros!=null){ros.add(consensusList, num);}
consensusList=new ArrayList<Read>(200);
num++;
}
}
if(consensusList.size()>0){
if(ros!=null){ros.add(consensusList, num);}
consensusList=new ArrayList<Read>(200);
num++;
}
if(graphList.size()>0 && ffmodel!=null){
// ReadWrite.writeObjectInThread(graphList, ffmodel.name(), true);
ReadWrite.writeAsync(graphList, ffmodel.name(), true);
}
}
@Override
public final void accumulate(ProcessThread pt){
readsProcessed+=pt.readsProcessedT;
basesProcessed+=pt.basesProcessedT;
alignedReads+=pt.alignedReadsT;
identitySum+=pt.identitySumT;
scoreSum+=pt.scoreSumT;
for(int i=0; i<idHist.length; i++){
idHist[i]+=pt.idHistT[i];
scoreHist[i]+=pt.scoreHistT[i];
}
errorState|=(!pt.success);
}
@Override
public final boolean success(){return !errorState;}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
public ByteBuilder toText() {
// TODO Auto-generated method stub
return null;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/** This class is static to prevent accidental writing to shared variables.
* It is safe to remove the static modifier. */
class ProcessThread extends Thread {
//Constructor
ProcessThread(final SamStreamer ss_, final int tid_){
ss=ss_;
cris=null;
tid=tid_;
realigner=(realign ? new Realigner() : null);
}
//Constructor
ProcessThread(final ConcurrentReadInputStream cris_, final int tid_){
ss=null;
cris=cris_;
tid=tid_;
realigner=null;//(realign ? new Realigner() : null);
}
//Called by start()
@Override
public void run(){
//Do anything necessary prior to processing
//Process the reads
processInner();
//Do anything necessary after processing
//Indicate successful exit status
success=true;
}
/** Iterate through the reads */
void processInner(){
//Grab and process all lists
if(ss!=null){
for(ListNum<Read> ln=ss.nextReads(); ln!=null; ln=ss.nextReads()){
processList(ln);
}
}else{
for(ListNum<Read> ln=cris.nextList(); ln!=null && ln.size()>0; ln=cris.nextList()){
processList(ln);
cris.returnList(ln);
}
}
}
void processList(ListNum<Read> ln){
//Loop through each read in the list
for(Read r : ln){
//Validate reads in worker threads
if(!r.validated()){r.validate(true);}
//Track the initial length for statistics
final int initialLength=r.length();
//Increment counters
readsProcessedT++;
basesProcessedT+=initialLength;
{
//Reads are processed in this block.
processRead(r);
}
}
}
/**
* Process a read or a read pair.
* @param r Read 1
* @param r2 Read 2 (may be null)
* @return True if the reads should be kept, false if they should be discarded.
*/
void processRead(final Read r){
if(r.bases==null || r.length()<=1){return;}
final SamLine sl=r.samline;
if(sl!=null && !sl.mapped()){return;}
final String rname=(sl==null ? defaultRname : sl.rnameS());
BaseGraph bg=refMap.get(rname);
if(bg==null){bg=refMap2.get(Tools.trimToWhitespace(rname));}
assert(bg!=null) : "Can't find graph for "+rname;
float identity;
float score=0;//unused
// assert(false) : r;
if(sl==null){
// identity=SketchObject.alignAndMakeMatch(r, bg.original);
// assert(false) : bg.refWeights[0];
// identity=SketchObject.alignAndMakeMatch(r, bg.original, bg.refWeights, bg.insWeights, bg.delWeights);
identity=SketchObject.alignAndMakeMatch(r, bg.original, bg.refWeights);
if(identity<=0){return;}
assert(r.match!=null) : identity+", "+r;
}else{
assert(sl!=null && sl.mapped() && sl.seq!=null) : sl;
assert(r.match!=null);
if(samFilter!=null && !samFilter.passesFilter(sl)){return;}
identity=sl.calcIdentity();
}
assert(r.match!=null && identity>0) : identity+", "+r;
if(inModel!=null){
if(verbose){System.err.println(r.start+"\t"+r.stop+"\t"+new String(r.match));}
score=inModel.score(r, false, true);
if(printScores){
// System.err.println(identity+"\t"+score+"\t"+inModel.score(r, false, true));
System.err.println(String.format("%.5f\t%.5f", identity, score));
}
// assert(false);
// if(score<0){
// verbose=true;
// inModel.score(r, false, false);
// }
// assert(!verbose);
}
alignedReadsT++;
identitySumT+=identity;
scoreSumT+=score;
idHistT[Tools.mid(0, Math.round(100*identity), 100)]++;
scoreHistT[Tools.mid(0, Math.round(100*score), 100)]++;
// assert(sl.calcIdentity()<=0.78) : sl.calcIdentity()+", "+samFilter.maxId;
// assert(false) : sl.cigar+", "+sl.calcIdentity();
// System.err.println(rname);
if(realigner!=null) {
assert(false);
realigner.realign(r, sl, bg.original, true);
}
bg.add(r);
}
long[] idHistT=new long[101];
long[] scoreHistT=new long[101];
/** Number of reads processed by this thread */
protected long readsProcessedT=0;
/** Number of bases processed by this thread */
protected long basesProcessedT=0;
protected long alignedReadsT=0;
double identitySumT=0;
double scoreSumT=0;
/** True only if this thread has completed successfully */
boolean success=false;
/** Shared input stream */
private final SamStreamer ss;
/** Alternate input stream */
private final ConcurrentReadInputStream cris;
/** Thread ID */
final int tid;
/** For realigning reads */
final Realigner realigner;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Read input file path */
private String in=null;
/** Reference input file path */
private String ref=null;
private String inModelFile;
private BaseGraph inModel;
private String outHistFile;
/** Consensus output file path */
private String out=null;
/** Model output file path */
private String outModel=null;
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
protected long alignedReads=0;
protected double identitySum=0;
protected double scoreSum=0;
/** Number of reads retained */
protected long readsOut=0;
/** Number of bases retained */
protected long basesOut=0;
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
public long subCount=0;
public long refCount=0;
public long delCount=0;
public long insCount=0;
long[] idHist=new long[101];
long[] scoreHist=new long[101];
boolean printScores=false;
/*--------------------------------------------------------------*/
/** Threads dedicated to reading the sam file */
private int streamerThreads=SamStreamer.DEFAULT_THREADS;
private String name=null;
private boolean loadedRef=false;
private boolean specialRef=false;
private boolean realign=false;
private int ploidy=1;
private final boolean silent;
public final SamFilter samFilter=new SamFilter();
/** Uses full ref names */
public LinkedHashMap<String, BaseGraph> refMap;
/** Uses truncated ref names */
public LinkedHashMap<String, BaseGraph> refMap2;
private String defaultRname=null;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Read input file */
private final FileFormat ffin;
/** Reference input file */
private final FileFormat ffref;
/** Consensus output file */
private final FileFormat ffout;
/** Model output file */
private final FileFormat ffmodel;
@Override
public final ReadWriteLock rwlock() {return rwlock;}
private final ReadWriteLock rwlock=new ReentrantReadWriteLock();
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
/** Reads ARE output in input order, even though this is false */
private final boolean ordered=false;
}
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