File: ReduceSilva.java

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package driver;

import java.io.PrintStream;
import java.util.ArrayList;
import java.util.HashSet;

import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tracker.ReadStats;

/**
 * @author Brian Bushnell
 * @date June 20, 2014
 *
 */
public class ReduceSilva {
	
	/*--------------------------------------------------------------*/
	/*----------------        Initialization        ----------------*/
	/*--------------------------------------------------------------*/
	
	/**
	 * Code entrance from the command line.
	 * @param args Command line arguments
	 */
	public static void main(String[] args){
		Timer t=new Timer();
		ReduceSilva x=new ReduceSilva(args);
		x.process(t);
		
		//Close the print stream if it was redirected
		Shared.closeStream(x.outstream);
	}
	
	/**
	 * Constructor.
	 * @param args Command line arguments
	 */
	public ReduceSilva(String[] args){
		
		{//Preparse block for help, config files, and outstream
			PreParser pp=new PreParser(args, getClass(), false);
			args=pp.args;
			outstream=pp.outstream;
		}
		
		Shared.capBuffers(4);
		ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
		ReadWrite.setZipThreads(Shared.threads());
		FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
		
		Parser parser=new Parser();
		for(int i=0; i<args.length; i++){
			String arg=args[i];
			String[] split=arg.split("=");
			String a=split[0].toLowerCase();
			String b=split.length>1 ? split[1] : null;

			if(parser.parse(arg, a, b)){
				//do nothing
			}else if(a.equals("verbose")){
				verbose=Parse.parseBoolean(b);
			}else if(a.equals("column")){
				column=Integer.parseInt(b);
			}else if(a.equals("parse_flag_goes_here")){
				//Set a variable here
			}else{
				outstream.println("Unknown parameter "+args[i]);
				assert(false) : "Unknown parameter "+args[i];
				//				throw new RuntimeException("Unknown parameter "+args[i]);
			}
		}
		
		{//Process parser fields
			Parser.processQuality();
			
			maxReads=parser.maxReads;
			
			overwrite=ReadStats.overwrite=parser.overwrite;
			append=ReadStats.append=parser.append;
			
			in1=parser.in1;

			out1=parser.out1;
			
			extin=parser.extin;
			extout=parser.extout;
		}
		
		assert(FastaReadInputStream.settingsOK());
		
		if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
		if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
			ByteFile.FORCE_MODE_BF2=true;
		}

		if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
		
		if(!Tools.testOutputFiles(overwrite, append, false, out1)){
			outstream.println((out1==null)+", "+out1);
			throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+"\n");
		}
		
		ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);

		ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
	}
	
	public boolean parseArgument(String arg, String a, String b){
		if(a.equals("reads") || a.equals("maxreads")){
			maxReads=Parse.parseKMG(b);
			return true;
		}else if(a.equals("some_argument")){
			maxReads=Parse.parseKMG(b);
			return true;
		}
		return false;
	}
	
	
	/*--------------------------------------------------------------*/
	/*----------------         Outer Methods        ----------------*/
	/*--------------------------------------------------------------*/

	/** Create read streams and process all data */
	void process(Timer t){
		
		final ConcurrentReadInputStream cris;
		{
			cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, null);
			cris.start();
			if(verbose){outstream.println("Started cris");}
		}
		boolean paired=cris.paired();
		if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}

		final ConcurrentReadOutputStream ros;
		if(out1!=null){
			final int buff=4;

			assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
			
			ros=ConcurrentReadOutputStream.getStream(ffout1, null, buff, null, false);
			ros.start();
		}else{ros=null;}
		
		readsProcessed=0;
		basesProcessed=0;
		
		readsOut=0;
		basesOut=0;
		
		//Process the read stream
		processInner(cris, ros);
		
		ReadWrite.closeStreams(cris, ros);
		if(verbose){outstream.println("Finished.");}
		
		errorState|=ReadStats.writeAll();
		errorState|=ReadWrite.closeStreams(cris, ros);
		
		t.stop();
		
		double rpnano=readsProcessed/(double)(t.elapsed);
		double bpnano=basesProcessed/(double)(t.elapsed);

		String rpstring=""+readsProcessed;
		String bpstring=""+basesProcessed;
		String rostring=""+readsOut;
		String bostring=""+basesOut;

		final int digits=10;
		while(rpstring.length()<digits){rpstring=" "+rpstring;}
		while(bpstring.length()<digits){bpstring=" "+bpstring;}
		while(rostring.length()<digits){rostring=" "+rostring;}
		while(bostring.length()<digits){bostring=" "+bostring;}
		
		outstream.println("Time:                         \t"+t);
		outstream.println("Reads Processed:    "+rpstring+" \t"+Tools.format("%.2fk reads/sec", rpnano*1000000));
		outstream.println("Bases Processed:    "+bpstring+" \t"+Tools.format("%.2fm bases/sec", bpnano*1000));
		outstream.println();
		outstream.println("Reads Out:          "+rostring+" \t"+Tools.format("%.2f%%", readsOut*100.0/readsProcessed));
		outstream.println("Bases Out:          "+bostring+" \t"+Tools.format("%.2f%%", basesOut*100.0/basesProcessed));
		
		if(errorState){
			throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
		}
	}
	
	/** Iterate through the reads */
	void processInner(final ConcurrentReadInputStream cris, final ConcurrentReadOutputStream ros){
		
		readsProcessed=0;
		basesProcessed=0;
		
		{
			
			ListNum<Read> ln=cris.nextList();
			ArrayList<Read> reads=(ln!=null ? ln.list : null);
			
			if(reads!=null && !reads.isEmpty()){
				Read r=reads.get(0);
				assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
			}

			while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
				if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
				
				for(int idx=0; idx<reads.size(); idx++){
					final Read r1=reads.get(idx);
					
					final int initialLength1=r1.length();
					
					{
						readsProcessed++;
						basesProcessed+=initialLength1;
					}
					
					boolean keep=processRead(r1);
					if(keep){
						readsOut++;
						basesOut+=initialLength1;
					}else{
						reads.set(idx, null);
					}
					
				}
				
				if(ros!=null){ros.add(reads, ln.id);}

				cris.returnList(ln);
				if(verbose){outstream.println("Returned a list.");}
				ln=cris.nextList();
				reads=(ln!=null ? ln.list : null);
			}
			if(ln!=null){
				cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
			}
		}
	}
	
	/*--------------------------------------------------------------*/
	/*----------------         Inner Methods        ----------------*/
	/*--------------------------------------------------------------*/
	
	/**
	 * Process a single read pair.
	 * @param r1 Read 1
	 * @return True if the read should be kept, false if it should be discarded.
	 */
	boolean processRead(final Read r1){
		String[] split=r1.id.split(";");
		if(split.length<=column){return true;}
		String taxa=split[split.length-column-1];
		boolean present=table.contains(taxa);
		if(present){return false;}
		table.add(taxa);
		return true;
	}
	
	/*--------------------------------------------------------------*/
	/*----------------            Fields            ----------------*/
	/*--------------------------------------------------------------*/
	
	private HashSet<String> table=new HashSet<String>();
	
	private String in1=null;

	private String out1=null;
	
	private String extin=null;
	private String extout=null;
	
	private int column=1;
	
	/*--------------------------------------------------------------*/

	protected long readsProcessed=0;
	protected long basesProcessed=0;
	
	protected long readsOut=0;
	protected long basesOut=0;
	
	private long maxReads=-1;
	
	/*--------------------------------------------------------------*/
	/*----------------         Final Fields         ----------------*/
	/*--------------------------------------------------------------*/
	
	private final FileFormat ffin1;

	private final FileFormat ffout1;
	
	/*--------------------------------------------------------------*/
	/*----------------        Common Fields         ----------------*/
	/*--------------------------------------------------------------*/
	
	private PrintStream outstream=System.err;
	public static boolean verbose=false;
	public boolean errorState=false;
	private boolean overwrite=true;
	private boolean append=false;
	
}