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package driver;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.concurrent.atomic.AtomicInteger;
import java.util.concurrent.atomic.AtomicLong;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
/**
* @author Brian Bushnell
* @date June 1, 2016
*
*/
public class RenameAndMux {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer();
RenameAndMux x=new RenameAndMux(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public RenameAndMux(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables
ReadWrite.USE_PIGZ=true;
ReadWrite.USE_UNPIGZ=false;
ReadWrite.USE_UNBGZIP=false;
ReadWrite.setZipThreads((Shared.threads()*3+1)/4);
//Create a parser object
Parser parser=new Parser();
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(!arg.contains("=")){
String[] x=(new File(arg).exists() ? new String[] {arg} : arg.split(","));
for(String x2 : x){readPaths.add(x2);}
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=parser.overwrite;
setInterleaved=parser.setInterleaved;
if(parser.in1!=null){
for(String s : parser.in1.split(",")){
readPaths.add(s);
}
}
out1=parser.out1;
out2=parser.out2;
extin=parser.extin;
extout=parser.extout;
}
//Do output file # replacement
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
// System.err.println("out1="+out1+", out2="+out2);
assert(FastaReadInputStream.settingsOK());
//Ensure there is an input file
if(readPaths.isEmpty()){throw new RuntimeException("Error - at least one input file is required.");}
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
if(out1==null){throw new RuntimeException("Error - output destination is required.");}
//Ensure out2 is not set without out1
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, false, false, out1, out2)){
outstream.println((out1==null)+", "+(out2==null)+", "+out1+", "+out2);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Reset counters
readsProcessedA.set(0);
basesProcessedA.set(0);
nextListNumber.set(0);
//Process the read stream
renameAndMerge_MT();
final long readsProcessed=readsProcessedA.get();
final long basesProcessed=basesProcessedA.get();
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
// private void renameAndMerge_ST(){
//
// long readsProcessed=0;
// long basesProcessed=0;
//
// FileFormat ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, false, false);
// FileFormat ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, false, false);
//
// for(String path : readPaths){
//
// String in1=null, in2=null;
//
// //Do input file # replacement
// if(path.indexOf('#')>-1 && !new File(path).exists()){
// in2=path.replace("#", "2");
// in1=path.replace("#", "1");
// }
//
// //Adjust interleaved settings based on number of output files
// if(!setInterleaved){
// assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
// if(in2!=null){ //If there are 2 input streams.
// FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
// if(!printedInterleavedMessage){
// outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
// printedInterleavedMessage=true;
// }
// }else{ //There is one input stream.
// if(out2!=null){
// FASTQ.FORCE_INTERLEAVED=true;
// FASTQ.TEST_INTERLEAVED=false;
// if(!printedInterleavedMessage){
// outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
// printedInterleavedMessage=true;
// }
// }
// }
// }
//
// //Ensure input files can be read
// if(!Tools.testInputFiles(false, true, in1, in2)){
// throw new RuntimeException("\nCan't read some input files.\n");
// }
//
// //Ensure that no file was specified multiple times
// if(!Tools.testForDuplicateFiles(true, in1, in2, out1, out2)){
// throw new RuntimeException("\nSome file names were specified multiple times.\n");
// }
//
// final FileFormat ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
// final FileFormat ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
// final ConcurrentReadInputStream cris;
// final String core=ReadWrite.stripToCore(in1);
// {
// cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2);
// if(verbose){outstream.println("Started cris");}
// cris.start(); //4567
// }
// boolean paired=cris.paired();
// if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
// final ConcurrentReadOutputStream ros;
//
// final int buff=4;
//
// if(cris.paired()){
// outstream.println("Writing interleaved.");
// }
//
// ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, buff, null, false);
// ros.start();
//
// {
//
// ListNum<Read> ln=cris.nextList();
// ArrayList<Read> reads=(ln!=null ? ln.list : null);
//
// if(reads!=null && !reads.isEmpty()){
// Read r=reads.get(0);
// assert((r.mate!=null)==cris.paired());
// }
//
// while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
//
// for(int idx=0; idx<reads.size(); idx++){
// final Read r1=reads.get(idx);
// final Read r2=r1.mate;
//
// final int initialLength1=r1.length();
// final int initialLength2=(r1.mateLength());
//
// {
// readsProcessed++;
// basesProcessed+=initialLength1;
// r1.id=core+"_"+r1.numericID+" /1";
// }
// if(r2!=null){
// readsProcessed++;
// basesProcessed+=initialLength2;
// r2.id=core+"_"+r1.numericID+" /2";
// }
//
//
// }
//
// final ArrayList<Read> listOut=reads;
//
// if(ros!=null){ros.add(listOut, ln.id);}
//
// cris.returnList(ln);
// ln=cris.nextList();
// reads=(ln!=null ? ln.list : null);
// }
// if(ln!=null){
// cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
// }
// }
//
// errorState|=ReadWrite.closeStreams(cris, ros);
//
// ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, null, true, false, true, false);
// ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, null, true, false, true, false);
// }
// readsProcessedA.addAndGet(readsProcessed);
// basesProcessedA.addAndGet(basesProcessed);
// }
private void renameAndMerge_MT(){
FileFormat ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, false, ordered);
FileFormat ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, false, ordered);
final ConcurrentReadOutputStream ros;
final int buff=4;
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, buff, null, false);
ros.start();
// System.err.println("Started ros.");
final int threads=Shared.threads();
ArrayList<MuxThread> list=new ArrayList<MuxThread>(threads);
for(int i=0; i<threads; i++){
MuxThread mt=new MuxThread(ros);
list.add(mt);
mt.start();
}
// System.err.println("Started threads.");
for(MuxThread mt : list){
while(mt.getState()!=Thread.State.TERMINATED){
try {
mt.join();
// System.err.println("Joined.");
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
// System.err.println(mt.getState());
}
// System.err.println("All threads finished.");
errorState|=ReadWrite.closeStream(ros);
}
void renameAndMergeOneFile(final String path, final ConcurrentReadOutputStream ros){
long readsProcessed=0;
long basesProcessed=0;
String in1=path, in2=null;
//Do input file # replacement
if(path.indexOf('#')>-1 && !new File(path).exists()){
in2=path.replace("#", "2");
in1=path.replace("#", "1");
}
synchronized(getClass()){
//Adjust interleaved settings based on number of output files
if(!setInterleaved){
assert(in1!=null && (out1!=null || out2==null)) : "\nin1="+in1+"\nin2="+in2+"\nout1="+out1+"\nout2="+out2+"\n";
if(in2!=null){ //If there are 2 input streams.
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
if(!printedInterleavedMessage){
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
printedInterleavedMessage=true;
}
}else{ //There is one input stream.
if(out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
if(!printedInterleavedMessage){
outstream.println("Set INTERLEAVED to "+FASTQ.FORCE_INTERLEAVED);
printedInterleavedMessage=true;
}
}
}
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1, in2)){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, in2, out1, out2)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
final FileFormat ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
final FileFormat ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
final ConcurrentReadInputStream cris;
final String core=ReadWrite.stripToCore(in1);
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ffin1, ffin2);
if(verbose){outstream.println("Started cris");}
cris.start(); //4567
}
boolean paired=cris.paired();
// if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((r.mate!=null)==cris.paired());
}
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
final Read r2=r1.mate;
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
{
readsProcessed++;
basesProcessed+=initialLength1;
r1.id=core+"_"+r1.numericID+" 1:";
}
if(r2!=null){
readsProcessed++;
basesProcessed+=initialLength2;
r2.id=core+"_"+r1.numericID+" 2:";
}
}
final ArrayList<Read> listOut=reads;
if(ros!=null){ros.add(listOut, 0/*nextListNumber.getAndIncrement()*/);}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
synchronized(getClass()){
errorState|=ReadWrite.closeStream(cris);
}
readsProcessedA.addAndGet(readsProcessed);
basesProcessedA.addAndGet(basesProcessed);
}
/*--------------------------------------------------------------*/
/*---------------- Nested Classes ----------------*/
/*--------------------------------------------------------------*/
private class MuxThread extends Thread{
MuxThread(ConcurrentReadOutputStream ros_){
ros=ros_;
}
@Override
public void run(){
for(int i=nextPathNumber.getAndIncrement(); i<readPaths.size(); i=nextPathNumber.getAndIncrement()){
renameAndMergeOneFile(readPaths.get(i), ros);
}
}
final ConcurrentReadOutputStream ros;
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
protected ArrayList<String> readPaths=new ArrayList<String>();
protected String out1, out2;
protected String extin;
protected String extout;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected AtomicLong readsProcessedA=new AtomicLong(0);
/** Number of bases processed */
protected AtomicLong basesProcessedA=new AtomicLong(0);
protected AtomicLong nextListNumber=new AtomicLong(0);
protected AtomicInteger nextPathNumber=new AtomicInteger(0);
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** This flag has no effect on singlethreaded programs */
private final boolean ordered=false;
/** Whether interleaved was explicitly set. */
private boolean setInterleaved=false;
private boolean printedInterleavedMessage=false;
}
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