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package driver;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.Collections;
import java.util.Comparator;
import java.util.HashMap;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextFile;
import fileIO.TextStreamWriter;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import tax.TaxNode;
import tax.TaxTree;
/**
* @author Brian Bushnell
* @date Oct 17, 2014
*
*/
public class SummarizeContamReport {
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
SummarizeContamReport x=new SummarizeContamReport(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public SummarizeContamReport(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Shared.capBuffers(4);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ReadWrite.verbose=verbose;
}else if(a.equals("minreads")){
minReads=Long.parseLong(b);
}else if(a.equals("minsequnits") || a.equals("minunits") || a.equals("minseqs")){
minSeqUnits=Long.parseLong(b);
}else if(a.equals("in")){
for(String term : b.split(",")){
in.add(term);
}
}else if(a.equals("tree")){
treeFile=b;
}else if(b==null && new File(arg).exists()){
in.add(arg);
}else if(parser.parse(arg, a, b)){
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
if("auto".equalsIgnoreCase(sizeFile)){sizeFile=TaxTree.defaultSizeFile();}
{//Process parser fields
Parser.processQuality();
overwrite=parser.overwrite;
append=parser.append;
out1=parser.out1;
}
if(in.isEmpty()){throw new RuntimeException("Error - at least one input file is required.");}
if(out1!=null && out1.equalsIgnoreCase("null")){out1=null;}
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+"\n");
}
ffout1=FileFormat.testOutput(out1, FileFormat.TEXT, null, true, overwrite, append, false);
ffinArray=new FileFormat[in.size()];
for(int i=0; i<in.size(); i++){
ffinArray[i]=FileFormat.testInput(in.get(i), FileFormat.TEXT, null, false, false);
}
tree=TaxTree.loadTaxTree(treeFile, System.err, true, false);
if(tree!=null){tree.loadSizeFile(sizeFile);}
}
void process(Timer t){
for(FileFormat ff : ffinArray){
if(ff.canRead()){
processOneFile(ff);
}else{
System.err.println("Skipping unreadable file "+ff.name());
}
}
printOutput();
t.stop();
outstream.println(Tools.timeLinesBytesProcessed(t, linesProcessed, charsProcessed, 8));
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
void processOneFile(FileFormat ff){
final TextFile tf;
{
tf=new TextFile(ff);
if(verbose){outstream.println("Started tf");}
}
{
String line;
line=tf.nextLine();
if(line.startsWith("CONTAM SUMMARY")){
line=tf.nextLine();
}
assert(line.startsWith("Examined ")) : line;
line=tf.nextLine();
assert(line.startsWith("|Taxonomy")) : line;
while(/*(maxReads<0 || linesProcessed<maxReads) && */(line=tf.nextLine())!=null){
linesProcessed++;
charsProcessed+=line.length();
if(line.startsWith("|")){
if(line.startsWith("|TOTAL")){break;}
processLine(line);
}else{
break;
}
}
}
errorState|=tf.close();
}
private void printOutput(){
final TextStreamWriter tsw;
{
tsw=new TextStreamWriter(ffout1);
tsw.start();
if(verbose){outstream.println("Started tsw");}
tsw.println("#Name\tSeqUnits\tReads\tTaxID\tClade\tsize\tcSize\tseqs\tcSeqs\tcNodes");
}
ArrayList<StringLongLong> list=new ArrayList<StringLongLong>(map.size());
list.addAll(map.values());
Collections.sort(list, new ComparatorA());
boolean filterA=minSeqUnits>1, filterB=minReads>1;
boolean filter=filterA || filterB;
for(StringLongLong sll : list){
if(sll.a>=minSeqUnits && sll.b>=minReads){
// if(!filter || (filterA && sll.a>=minSeqUnits) || (filterB && sll.b>=minReads)){
int tid=-1;
TaxNode tn;
// TaxNode tn=null;
TaxNode ancestor=null;
long size=0;
long cumulative_size=0;
long seqs=0;
long cumulative_seqs=0;
long cumulative_nodes=0;
if(tree!=null){
tid=tree.parseNameToTaxid(sll.s);
if(tid>=0){
tn=tree.getNode(tid);
ancestor=tree.getNodeAtLevelExtended(tid, TaxTree.SUPERKINGDOM_E);
size=tree.toSize(tn);
cumulative_size=tree.toSizeC(tn);
seqs=tree.toSeqs(tn);
cumulative_seqs=tree.toSeqsC(tn);
cumulative_nodes=tree.toNodes(tn);
}
}
tsw.println(sll.s+"\t"+sll.a+"\t"+sll.b+"\t"+tid+"\t"+(ancestor==null ? "null" : ancestor.name)+
"\t"+size+"\t"+cumulative_size+"\t"+seqs+"\t"+cumulative_seqs+"\t"+cumulative_nodes);
}
}
errorState|=tsw.poisonAndWait();
}
private void processLine(String line){
String[] split=line.split("\\|");
String[] split2=split[1].split(";");
String name=split2[split2.length-1];
try {
long a=Long.parseLong(split[2]);
long b=Long.parseLong(split[3]);
StringLongLong p=map.get(name);
if(p==null){
p=new StringLongLong(name, a, b);
map.put(name, p);
}else{
p.a+=a;
p.b+=b;
}
} catch (NumberFormatException e) {
// TODO Auto-generated catch block
e.printStackTrace();
System.err.println(line);
System.err.println(Arrays.toString(split));
System.err.println(Arrays.toString(split2));
shared.KillSwitch.kill();
}
}
/*--------------------------------------------------------------*/
class ComparatorA implements Comparator<StringLongLong> {
@Override
public int compare(StringLongLong x, StringLongLong y) {
if(x.a!=y.a){return x.a<y.a ? 1 : -1;}
if(x.b!=y.b){return x.b<y.b ? 1 : -1;}
return x.s.compareTo(y.s);
}
}
class ComparatorB implements Comparator<StringLongLong> {
@Override
public int compare(StringLongLong x, StringLongLong y) {
if(x.b!=y.b){return x.b<y.b ? 1 : -1;}
if(x.a!=y.a){return x.a<y.a ? 1 : -1;}
return x.s.compareTo(y.s);
}
}
class StringLongLong {
StringLongLong(String s_){
s=s_;
}
StringLongLong(String s_, long a_, long b_){
s=s_;
a=a_;
b=b_;
}
final String s;
long a;
long b;
}
/*--------------------------------------------------------------*/
private ArrayList<String> in=new ArrayList<String>();
private String out1=null;
private String treeFile="auto";
private String sizeFile="auto";
TaxTree tree=null;
private HashMap<String, StringLongLong> map=new HashMap<String, StringLongLong>();
/*--------------------------------------------------------------*/
long minReads=0;
long minSeqUnits=0;
long linesProcessed=0;
long charsProcessed=0;
/*--------------------------------------------------------------*/
private final FileFormat ffinArray[];
private final FileFormat ffout1;
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
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