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package driver;
import java.io.File;
import java.util.ArrayList;
import java.util.Collections;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import structures.ByteBuilder;
import tracker.SealStats;
import tracker.SealStats.SealStatsLine;
/**
* @author Brian Bushnell
* @date August 15, 2023
*
*/
public class SummarizeSealCrosstalk {
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
SummarizeSealCrosstalk x=new SummarizeSealCrosstalk(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public SummarizeSealCrosstalk(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
Parser parser=new Parser();
parser.out1=out1;
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(a.equals("primary") || a.equals("useprimary")){
usePrimary=Parse.parseBoolean(b);
}else if(arg.indexOf('=')<0 && new File(arg).exists()){
in.add(arg);
}else if(parser.parse(arg, a, b)){
//do nothing
}else{
// throw new RuntimeException("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
outstream.println("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
overwrite=parser.overwrite;
append=parser.append;
if(append) {overwrite=false;}
if(parser.in1!=null){
Tools.addFiles(parser.in1, in);
}
out1=parser.out1;
}
ffout1=FileFormat.testOutput(out1, FileFormat.TXT, null, true, overwrite, append, false);
}
void process(Timer t){
ArrayList<byte[]> header=new ArrayList<byte[]>();
// ArrayList<byte[]> results=new ArrayList<byte[]>();
ArrayList<Result> results=new ArrayList<Result>();
ByteBuilder bb=new ByteBuilder();
bb.append("#All values are in read PPM.\n");
bb.append("#Files");
for(String s : in) {bb.tab().append(s);}
//header.add(bb.nl().toBytes());
bb.clear();
Result totals=new Result("#Totals");
for(String fname : in) {
SealStats ss=new SealStats(fname);
String core=ss.fnamePrefix();
core=core.replace("stats_", "").replace("_stats", "");
SealStatsLine primary=(usePrimary ? ss.primary() : ss.map.get(core));
String name=(usePrimary && primary!=null ? primary.name : core);
SealStatsLine contam=ss.countNonmatching(name);
long primaryReads=(primary==null ? 0 : primary.reads);
long contamReads=(contam==null ? 0 : contam.reads);
Result r=new Result(core, ss.totalReads, ss.matchedReads, primaryReads, contamReads);
totals.add(r);
results.add(r);
}
if(verbose){outstream.println("Finished reading data.");}
{
totals.appendTo(bb);
header.add(bb.nl().toBytes());
bb.clear();
bb.append("#Name\tCorrect\tContam\tAmbig");
header.add(bb.nl().toBytes());
bb.clear();
}
outputResults(header, results);
t.stop();
outstream.println("Time: \t"+t);
// outstream.println("Reads Processed: "+readsProcessed+" \t"+Tools.format("%.2fk reads/sec", (readsProcessed/(double)(t.elapsed))*1000000));
// assert(!errorState) : "An error was encountered.";
}
private void outputResults(ArrayList<byte[]> header, ArrayList<Result> results){
ByteStreamWriter bsw=new ByteStreamWriter(ffout1);
bsw.start();
ByteBuilder bb=new ByteBuilder();
for(byte[] line : header) {bsw.print(line);}
Collections.sort(results);
// Collections.reverse(results);
for(Result r : results) {
r.appendTo(bb).nl();
bsw.print(bb);
bb.clear();
}
errorState=bsw.poisonAndWait() | errorState;
}
private static class Result implements Comparable<Result> {
Result(String name_){
this(name_, 0, 0, 0, 0);
}
Result(String name_, long total_, long matched_, long primary_, long contam_){
name=name_;
total=total_;
matched=matched_;
primary=primary_;
contam=contam_;
ambig=total-matched;
}
Result add(Result r) {
total+=r.total;
matched+=r.matched;
primary+=r.primary;
contam+=r.contam;
ambig+=r.ambig;
return this;
}
ByteBuilder appendTo(ByteBuilder bb) {
double inv=1.0/(Tools.max(1, total));
double ppmMult=inv*1000000;
bb.append(name).tab();//Could use name here instead
bb.append(primary*ppmMult, 2).tab();
bb.append(contam*ppmMult, 2).tab();
bb.append(ambig*ppmMult, 2);
return bb;
}
@Override
public int compareTo(Result o) {
double inv1=1.0/(Tools.max(1, total));
double inv2=1.0/(Tools.max(1, o.total));
if(contam*inv1!=o.contam*inv2) {return contam*inv1>o.contam*inv2 ? 1 : -1;}
if(primary*inv1!=o.primary*inv2) {return primary*inv1>o.primary*inv2 ? 1 : -1;}
if(ambig*inv1!=o.ambig*inv2) {return ambig*inv1>o.ambig*inv2 ? 1 : -1;}
return name.compareTo(o.name);
}
String name;
long total;
long matched;
long primary;
long contam;
long ambig;
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
private ArrayList<String> in=new ArrayList<String>();
private String out1="stdout.txt";
private final FileFormat ffout1;
/*--------------------------------------------------------------*/
private boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
private boolean usePrimary=false;
/*--------------------------------------------------------------*/
private java.io.PrintStream outstream=System.err;
public static boolean verbose=false;
}
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