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package gff;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.HashMap;
import fileIO.ByteFile;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import prok.PGMTools;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.Read;
import stream.ReadInputStream;
public class CutGff_ST {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
CutGff_ST x=new CutGff_ST(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public CutGff_ST(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, null/*getClass()*/, false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
Shared.TRIM_READ_COMMENTS=Shared.TRIM_RNAME=true;
GffLine.parseAttributes=true;
{//Parse the arguments
final Parser parser=parse(args);
overwrite=parser.overwrite;
append=parser.append;
out=parser.out1;
}
fixExtensions(); //Add or remove .gz or .bz2 as needed
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
ffout=FileFormat.testOutput(out, FileFormat.PGM, null, true, overwrite, append, false);
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
Parser parser=new Parser();
parser.overwrite=overwrite;
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
// outstream.println(arg+", "+a+", "+b);
if(PGMTools.parseStatic(arg, a, b)){
//do nothing
}else if(a.equals("in") || a.equals("infna") || a.equals("fnain") || a.equals("fna") || a.equals("ref")){
assert(b!=null);
Tools.addFiles(b, fnaList);
}else if(a.equals("gff") || a.equals("ingff") || a.equals("gffin")){
assert(b!=null);
Tools.addFiles(b, gffList);
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
ReadWrite.verbose=verbose;
}else if(a.equals("invert")){
invert=Parse.parseBoolean(b);
}else if(a.equals("type") || a.equals("types")){
types=b;
}else if(a.equals("attributes") || a.equals("requiredattributes")){
requiredAttributes=b.split(",");
}else if(a.equals("banattributes") || a.equals("bannedattributes")){
bannedAttributes=b.split(",");
}else if(a.equals("banpartial")){
banPartial=Parse.parseBoolean(b);
}
else if(a.equals("minlen")){
minLen=Integer.parseInt(b);
}else if(a.equals("maxlen")){
maxLen=Integer.parseInt(b);
}
else if(parser.parse(arg, a, b)){
//do nothing
}else if(arg.indexOf('=')<0 && new File(arg).exists() && FileFormat.isFastaFile(arg)){
fnaList.add(arg);
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
ArrayList<String> banned=new ArrayList<String>();
if(banPartial){banned.add("partial=true");}
if(bannedAttributes!=null){
for(String s : bannedAttributes){banned.add(s);}
}
bannedAttributes=banned.isEmpty() ? null : banned.toArray(new String[0]);
if(gffList.isEmpty()){
for(String s : fnaList){
String prefix=ReadWrite.stripExtension(s);
String gff=prefix+".gff";
File f=new File(gff);
if(!f.exists()){
String gz=gff+".gz";
f=new File(gz);
assert(f.exists() && f.canRead()) : "Can't read file "+gff;
gff=gz;
}
gffList.add(gff);
}
}
assert(gffList.size()==fnaList.size()) : "Number of fna and gff files do not match: "+fnaList.size()+", "+gffList.size();
return parser;
}
/** Add or remove .gz or .bz2 as needed */
private void fixExtensions(){
fnaList=Tools.fixExtension(fnaList);
gffList=Tools.fixExtension(gffList);
if(fnaList.isEmpty()){throw new RuntimeException("Error - at least one input file is required.");}
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out)){
outstream.println((out==null)+", "+out);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output file "+out+"\n");
}
//Ensure input files can be read
ArrayList<String> foo=new ArrayList<String>();
foo.addAll(fnaList);
foo.addAll(gffList);
if(!Tools.testInputFiles(false, true, foo.toArray(new String[0]))){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
foo.add(out);
if(!Tools.testForDuplicateFiles(true, foo.toArray(new String[0]))){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
}
/*--------------------------------------------------------------*/
/*---------------- Actual Code ----------------*/
/*--------------------------------------------------------------*/
public void process(Timer t){
ByteStreamWriter bsw=new ByteStreamWriter(ffout);
bsw.start();
for(int i=0; i<fnaList.size(); i++){
processFile(fnaList.get(i), gffList.get(i), types, bsw);
}
bsw.poisonAndWait();
}
private void processFile(String fna, String gff, String types, ByteStreamWriter bsw){
ArrayList<GffLine> lines=GffLine.loadGffFile(gff, types, false);
ArrayList<Read> list=ReadInputStream.toReads(fna, FileFormat.FA, -1);
HashMap<String, Read> map=new HashMap<String, Read>();
for(Read r : list){map.put(r.id, r);}
processStrand(lines, map, 0, bsw, invert);
for(Read r : list){r.reverseComplement();}
processStrand(lines, map, 1, bsw, invert);
if(invert){
for(Read r : list){
r.reverseComplement();
bsw.println(r);
}
}
}
private boolean hasAttributes(GffLine gline){
int len=gline.length();
if(len<minLen || len>maxLen){return false;}
if(hasAttributes(gline, bannedAttributes)){return false;}
return requiredAttributes==null || hasAttributes(gline, requiredAttributes);
}
private boolean hasAttributes(GffLine gline, String[] attributes){
if(attributes==null){return false;}
for(String s : attributes){
if(gline.attributes.contains(s)){
return true;
}
}
return false;
}
private void processStrand(ArrayList<GffLine> lines, HashMap<String, Read> map, int strand, ByteStreamWriter bsw, boolean invert){
for(GffLine gline : lines){
if(gline.strand==strand && hasAttributes(gline)){
Read scaf=map.get(gline.seqid);
assert(scaf!=null) : "Can't find "+gline.seqid+" in "+map.keySet();
int start, stop;
if(strand==0){
start=gline.start-1;
stop=gline.stop-1;
}else{
start=scaf.length()-gline.stop-1;
stop=scaf.length()-gline.start-1;
}
if(invert){
byte[] bases=scaf.bases;
for(int i=start; i<stop; i++){
if(i>=0 && i<bases.length){
bases[i]='N';
}
}
}else{
if(start>=0 && stop<scaf.length()){
Read r=new Read(Arrays.copyOfRange(scaf.bases, start, stop), null, gline.attributes, 1);
bsw.println(r);
}
}
}
}
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
private ArrayList<String> fnaList=new ArrayList<String>();
private ArrayList<String> gffList=new ArrayList<String>();
private String out=null;
private String types="CDS";
private boolean invert=false;
private boolean banPartial=true;
private int minLen=1;
private int maxLen=Integer.MAX_VALUE;
private String[] requiredAttributes;
private String[] bannedAttributes;
/*--------------------------------------------------------------*/
private long bytesOut=0;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
private final FileFormat ffout;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
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