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package hiseq;
import shared.KillSwitch;
import shared.Tools;
import structures.ByteBuilder;
/**
* Parses an Illumina header to gather positional information.
* Superceded by ihp2.
* @author Brian Bushnell
* @date Aug 22, 2018
*
*/
public class IlluminaHeaderParser1 extends ReadHeaderParser {
/*--------------------------------------------------------------*/
/*---------------- Main ----------------*/
/*--------------------------------------------------------------*/
public static void main(String[] args) {
PARSE_COMMENT=true;
IlluminaHeaderParser1 ihp=new IlluminaHeaderParser1();
ihp.test(args.length>0 ? args[0] : null);
}
/*--------------------------------------------------------------*/
/*---------------- Expected Format ----------------*/
/*--------------------------------------------------------------*/
//@VP2-06:112:H7LNDMCVY:2:2437:14181:20134 (Novaseq6k)
//2402:6:1101:6337:2237/1
//MISEQ08:172:000000000-ABYD0:1:1101:18147:1925 1:N:0:TGGATATGCGCCAATT
//HISEQ07:419:HBFNEADXX:1:1101:1238:2072
//A00178:38:H5NYYDSXX:2:1101:3007:1000 1:N:0:CAACCTA+CTAGGTT
//@LH00223:28:22GLGMLT3:1:1101:5928:1016 1:N:0:CTGCTTGGTT+CTAACGACAG (NovaseqX)
// @HWI-Mxxxx or @Mxxxx - MiSeq
// @HWUSI - GAIIx
// @HWI-Dxxxx - HiSeq 2000/2500
// @Kxxxx - HiSeq 3000(?)/4000
// @Nxxxx - NextSeq 500/550
// @Axxxxx - NovaSeq
// @Vxxxxx = NextSeq 2000
// @AAxxxxx - NextSeq 2000 P1/P2/P3
// @Hxxxxxx - NovaSeq S1/S2/S4
//
// AAXX = Genome Analyzer
// BCXX = HiSeq v1.5
// ACXX = HiSeq High-Output v3
// ANXX = HiSeq High-Output v4
// ADXX = HiSeq RR v1
// AMXX, BCXX =HiSeq RR v2
// ALXX = HiSeqX
// BGXX, AGXX = High-Output NextSeq
// AFXX = Mid-Output NextSeq
// 5 letter/number = MiSeq
/*--------------------------------------------------------------*/
/*---------------- Public Methods ----------------*/
/*--------------------------------------------------------------*/
public IlluminaHeaderParser1 parse(String id_) {
reset(id_);
try {
commentSeparator=findCommentSeparator();
if(PARSE_COORDINATES){parseCoordinates();}
if(PARSE_COMMENT){parseComment();}
} catch (Throwable e) {
System.err.println("Trouble parsing header "+id_);
KillSwitch.throwableKill(e);
}
return this;
}
@Override
public String machine() {
return null;
}
@Override
public String sample() {
return null;
}
@Override
public int run() {
return -1;
}
@Override
public String flowcell() {
return null;
}
@Override
public int lane() {return lane;}
@Override
public int tile() {return tile;}
@Override
public int xPos() {return x;}
@Override
public int yPos() {return y;}
@Override
public char pairCode() {return pairCode;}
@Override
public char chastityCode() {return chastityCode;}
@Override
public int controlBits() {return controlBits;}
@Override
public String barcode() {
assert(PARSE_COMMENT);
return barcode;
}
@Override
public String extra() {
assert(PARSE_COMMENT);
return extra;
}
/*--------------------------------------------------------------*/
/*---------------- Private Methods ----------------*/
/*--------------------------------------------------------------*/
/** Parse lane, tile, x, and y coordinates */
private void parseCoordinates(){
pos=commentSeparator;
goBackSeveralColons(4);
lane=parseInt();
if(!Tools.isDigit(id.charAt(pos))){//Hiseq 3000? I'm not really sure what the header looks like for this block
while(pos<limit && id.charAt(pos)!=':'){pos++;}
pos++;
lane=parseInt();
}
tile=parseInt();
x=parseInt();
y=parseInt();
}
/** Parse the comment field for pair number, chastity filter, and barcode */
private void parseComment(){
pos=commentSeparator+1;
pairCode=parseChar();
chastityCode=parseChar();
controlBits=parseInt();
int idx=id.indexOf(' ', pos);
idx=(idx>=0 ? idx : id.indexOf('\t', pos));
if(idx<0) {
barcode=id.substring(pos);
extra=null;
}else {
barcode=id.substring(pos, idx);
extra=id.substring(idx+1);
}
}
/** Clear all fields and point to a new header */
private void reset(String id_){
id=id_;
limit=(id==null ? -1 : id.length());
pos=-1;
commentSeparator=-1;
lane=-1;
tile=-1;
x=-1;
y=-1;
pairCode='?';
barcode=null;
chastityCode='?';
controlBits=-1;
}
/*--------------------------------------------------------------*/
/*---------------- Helper Methods ----------------*/
/*--------------------------------------------------------------*/
/**
* Find the first instance of the comment separator (' ' or '/')
* @return Position of the separator, or header length if not found
*/
private int findCommentSeparator(){
for(int i=0; i<limit; i++){
char c=id.charAt(i);
if(c==' ' || c== '/'){return i;}
}
return limit;
}
/**
* Decrement pos until target colons have been encountered
* @modifies pos
* @param target number of colons to backtrack
*/
private void goBackSeveralColons(int target){
for(int colons=0; pos>=0; pos--){
if(id.charAt(pos)==':'){
colons++;
if(colons==target){break;}
}
}
pos++;
}
/**
* Parse an integer from the current location in the string,
* and advance the position to the beginning of the next number.
* @modifies pos
* @return The parsed number
*/
private int parseInt(){
int current=0;
assert(Tools.isDigit(id.charAt(pos))) : id;
while(pos<limit && Tools.isDigit(id.charAt(pos))){
current=current*10+(id.charAt(pos)-'0');
pos++;
}
pos++;
return current;
}
/**
* Parse a character from the current location in the string,
* and advance the position to the next code character.
* @modifies pos
* @return The parsed char
*/
private char parseChar(){
char c=id.charAt(pos);
assert(c!=':');
pos++;
assert(pos>=limit || id.charAt(pos)==':');
pos++;
assert(pos>=limit || id.charAt(pos)!=':');
return c;
}
public String toString() {
ByteBuilder bb=new ByteBuilder();
bb.append("lane:\t").append(lane).nl();
bb.append("tile:\t").append(tile).nl();
bb.append("x:\t").append(x).nl();
bb.append("y:\t").append(y).nl();
bb.append("pairnum:\t").append(pairCode).nl();
bb.append("barcode:\t").append(barcode).nl();
bb.append("chastity:\t").append(chastityCode).nl();
return bb.toString();
}
/*--------------------------------------------------------------*/
/*---------------- Public Fields ----------------*/
/*--------------------------------------------------------------*/
/** Flowcell lane number */
public int lane;
/** Tile number */
public int tile;
/** X-coordinate within tile (pixels) */
public int x;
/** Y-coordinate within tile (pixels) */
public int y;
/** '1' for read 1, '2' for read 2 */
public char pairCode;
/** 'Y' for fail, 'N' for pass */
public char chastityCode;
/** A number for control bits set */
public int controlBits;
/** Read barcode */
public String barcode;
/** Anything after the barcode */
public String extra;
/*--------------------------------------------------------------*/
/*---------------- Private Fields ----------------*/
/*--------------------------------------------------------------*/
/** Length of header */
private int limit;
/** Current position, typically the start of the next token */
private int pos;
/** Position after coordinates, but before pairnum; typically space or slash */
private int commentSeparator;
}
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