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package jasper;
import java.io.FileNotFoundException;
import java.io.IOException;
import java.util.HashSet;
import shared.Parser;
import shared.PreParser;
import shared.Timer;
public class DenseTreeValidate {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
public static void main(String[] args) throws FileNotFoundException, IOException {
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
DenseTreeValidate x=new DenseTreeValidate(args);
//Run the object
x.process(t);
}
/**
* Handles pre-parsing and parsing of user flags.
* Reads in the sketch similarity file (sim) and organism-parent relationship file (tree).
*
* @param args string of the arguments input at the commandline.
*/
public DenseTreeValidate(String[] args) {
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Primary parsing of standard arguments found in all bbmap programs (maxReads, parseSam, parseZip, etc).
Parser parser=new Parser();
//Loop through arguments up to the maximum number of arguments input.
//process all remaining arguments.
for(int i=0; i<args.length; i++){
//Grab argument string at index.
String arg=args[i];
//Split argument string on "=".
String[] split=arg.split("=");
//Convert the left side to lowercase.
String a=split[0].toLowerCase();
//Ternary conditional statement: is the length of the split greater than 1 (thus, an actual input)?
//if so, the right side of the split is the b variable, if not, b is null.
String b=split.length>1 ? split[1] : null;
//If b isn't null but a string "null" was input, convert b to null.
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
//Unused example statement. does nothing currently. start here for adding new flag parsing.
if(a.equals("parse_flag_goes_here")){
//Handle reference variable assignment.
}else if(a.equals("sim")){
sim=b;
//Handle kmer variable assignment.
}else if(a.equals("tree")){
tree=b;
//Parses in and out flags, handles all flags not recognized earlier in class.
}else if(parser.parse(arg, a, b)){
//If not one of the known parameters, let the user know they made a mistake.
}else{
assert(false) : "Unknown parameter "+args[i];
outstream.println("Unknown parameter "+args[i]);
}
}
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/**
* Creates the similarity matrix and the relationship tree.
* Also passes these objects to the inner processing method for analysis.
*
* @param t
* @throws FileNotFoundException
* @throws IOException
*/
void process(Timer t) throws FileNotFoundException, IOException{
//Pass input file to Tree class to create tree
DenseTree relationshipTree=new DenseTree(tree);
//Pass similarity file to create similarity matrix object
DenseSimilarityMatrix matrix=new DenseSimilarityMatrix(sim, relationshipTree);
//Add parent node similarity percentages to each node in the tree.
//addRelationSims(relationshipTree, matrix);
//Traverse the tree and add levels to all nodes.
//Hardcoded to start at node "0" or "life" node.
relationshipTree.beginTraverse("0");
//relationshipTree.setIdentity(relationshipTree.getNode(10), matrix);
//TODO: remove 10 since this is just part of testing/percolate
//relationshipTree.root.percolateIdentityUp(10);
//Check similarities.
checkSimilarities(relationshipTree, matrix);
//System.out.println(relationshipTree);
t.stop();
outstream.println("Time: \t"+t);
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
// /**
// * Iterate over nodes in the Tree and compare present relationships
// * with values found in the similarity matrix.
// *
// * @param tree Tree object containing TreeNode objects detailing the parent and children of each node.
// * @param matrix SimilarityMatrix2 object containing percentage similarity of sketches.
// */
// void addRelationSims(DenseTree tree, DenseSimilarityMatrix matrix){
//
// //Iterate over organisms/nodes in the tree.
// for ( String keyOrg : tree.keySet() ) {
//
// TreeNode keyNode = tree.getNode(keyOrg);
//
// //Identify parent node.
// String parentName = keyNode.getParentName();
//
// //Get descendant nodes.
// HashSet<String> childNames = keyNode.getChildren();
//
// if(!parentName.equals("0")) {
//
// double parSim = matrix.getSimilarity(keyOrg, parentName);
//
// keyNode.addParSim(parSim);
// }
//
// for(String kid : childNames) {
//
// if(!tree.getNode(kid).parentName.equals("0")) {
//
// double kidSim = matrix.getSimilarity(keyOrg, kid);
//
// keyNode.addChildSim(kid, kidSim);
// }
// }
// }
// }
void checkSimilarities(DenseTree tree, DenseSimilarityMatrix matrix) {
//Iterate over organisms/nodes in the tree.
for ( String keyOrgName : tree.keySet() ) {
//If the organism isn't the life/0 node.
if(!keyOrgName.equals("0")) {
//System.out.println("key org " + keyOrg);
//Get the node from the tree
TreeNode keyNode = tree.getNode(keyOrgName);
int keyNodeID = keyNode.getNodeId();
tree.root.resetIdentity();
tree.setIdentity(keyNode, matrix);
tree.root.percolateIdentityUp(keyNode.nodeId);
//Prevents analysis of "empty" nodes that don't contain sequences (genus/phylum/etc).
//TODO: if there are no sibling nodes, the parent sim could be 0.
if(keyNode.parentNode.averageIdentity() != 0.0) {
//Identify parent node.
String parentName = keyNode.getParentName();
double parentSimilarity = matrix.getSimilarity(keyOrgName, parentName);
//Get the child node names.
HashSet<String> childNameSet = keyNode.getChildren();
//Iterate over the organisms in the matrix.
for(String matrixOrg : tree.keySet()) {
TreeNode matrixOrgNode = tree.getNode(matrixOrg);
//if we aren't comparing similarities of the node to itself and
//if we aren't examining a child node and
//if we aren't examining a parent node
if(!matrixOrgNode.isDescendantOf(keyNode) && !matrixOrgNode.isAncestorOf(keyNode) && !matrixOrg.equals(parentName) ) {
double matrixOrgSim = matrix.getSimilarity(keyOrgName, matrixOrg);
if(matrixOrgSim > parentSimilarity) {
System.out.println();
System.out.println("problem");
System.out.println("key org " + keyOrgName);
//System.out.println("kid name " + minChildName);
System.out.println("par name " + parentName);
System.out.println("other org " + matrixOrg);
System.out.println("par sim " + parentSimilarity);
//System.out.println("child sim " + minChildSim);
System.out.println("matrix sim " + matrixOrgSim);
keyNode.flagRelation(matrixOrg, matrixOrgSim);
System.out.println(keyNode.getFlaggedRelations());
}
}
}
}
}
}
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
private String sim=null;
private String tree=null;
private String out=null;
/*--------------------------------------------------------------*/
private long linesProcessed=0;
private long linesOut=0;
private long bytesProcessed=0;
private long bytesOut=0;
private long taxa=0;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
private String[] header;
//private final FileFormat ffin;
//private final FileFormat ffout;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
//private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
/**Output stream that output statistics are piped through to the output file. */
private java.io.PrintStream outstream=System.err;
}
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