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package ml;
import java.util.ArrayList;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.Read;
import structures.ListNum;
/**
* @author Brian Bushnell
* @date June 1, 2016
*
*/
public class ProcessBBMergeHeaders {
public static void main(String[] args){
Timer t=new Timer();
ProcessBBMergeHeaders x=new ProcessBBMergeHeaders(args);
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
public ProcessBBMergeHeaders(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
FASTQ.TEST_INTERLEAVED=false;
FASTQ.FORCE_INTERLEAVED=false;
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(parser.parse(arg, a, b)){
//do nothing
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
in1=parser.in1;
out1=parser.out1;
}
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, null, true, true, false, false);
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
}
void process(Timer t){
final ConcurrentReadInputStream cris;
{
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, false, ffin1, null);
cris.start();
}
final ConcurrentReadOutputStream ros;
if(out1!=null){
final int buff=4;
assert(!out1.equalsIgnoreCase(in1) && !out1.equalsIgnoreCase(in1)) : "Input file and output file have same name.";
ros=ConcurrentReadOutputStream.getStream(ffout1, null, buff, null, false);
ros.start();
}else{ros=null;}
long readsProcessed=0;
{
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
ArrayList<Read> keep=new ArrayList<Read>(reads.size());
keep.add(new Read(null, null, headerString(), 0));
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose){outstream.println("Fetched "+reads.size()+" reads.");}
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
Header h=makeHeader(r1.id);
if(h!=null){
r1.id=h.toString();
keep.add(r1);
}
readsProcessed++;
}
if(ros!=null){ros.add(keep, ln.id);}
keep=new ArrayList<Read>();
cris.returnList(ln);
if(verbose){outstream.println("Returned a list.");}
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
ReadWrite.closeStreams(cris, ros);
if(verbose){outstream.println("Finished.");}
t.stop();
outstream.println("Time: \t"+t);
outstream.println("Reads Processed: "+readsProcessed+" \t"+Tools.format("%.2fk reads/sec", (readsProcessed/(double)(t.elapsed))*1000000));
}
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
private Header makeHeader(String line){
if(!line.startsWith("insert=")){return null;}
if(!line.contains(" mo=")){return null;}
Header h=new Header(line);
return h.valid ? h : null;
}
public String headerString(){
return "#Correct\tminOverlap\tbestOverlap\tbestBadInt\tsecondBestOverlap\tsecondBestBadInt\t"
+ "expectedErrors\tbestExpectedErrors\tbestRatio\tbestBad\tsecondBestRatio\tsecondBestBad\tprobability";
}
private class Header {
//mo=14_r1ee=5.2728_r2ee=3.4856_bi=202_bo=98_bb=5.3063_br=0.0598_bbi=6_sbi=270_sbo=30_sbb=12.4775_sbr=0.4343_sbbi=14_be=6.5990_pr=0.0007
Header(String line_){
line=line_;
String[] split=line.split(" ");
trueInsert=Integer.parseInt(split[0].split("=")[1]);
split=split[2].split("_");
for(String s : split){
String[] split2=s.split("=");
String a=split2[0], b=split2[1];
if(a.equals("mo")){
minOverlap=Integer.parseInt(b);
}else if(a.equals("bi")){
bestInsert=Integer.parseInt(b);
}else if(a.equals("bo")){
bestOverlap=Integer.parseInt(b);
}else if(a.equals("bbi")){
bestBadInt=Integer.parseInt(b);
}else if(a.equals("sbi")){
secondBestInsert=Integer.parseInt(b);
}else if(a.equals("sbo")){
secondBestOverlap=Integer.parseInt(b);
}else if(a.equals("sbbi")){
secondBestBadInt=Integer.parseInt(b);
}else if(a.equals("r1ee")){
expectedErrors1=Float.parseFloat(b);
}else if(a.equals("r2ee")){
expectedErrors2=Float.parseFloat(b);
}else if(a.equals("be")){
bestExpected=Float.parseFloat(b);
}else if(a.equals("pr")){
probability=Float.parseFloat(b);
}else if(a.equals("br")){
bestRatio=Float.parseFloat(b);
}else if(a.equals("bb")){
bestBad=Float.parseFloat(b);
}else if(a.equals("sbr")){
secondBestRatio=Float.parseFloat(b);
}else if(a.equals("sbb")){
secondBestBad=Float.parseFloat(b);
}else{
throw new RuntimeException(s);
}
}
correct=(bestInsert==trueInsert);
valid=(split.length==15 && bestInsert>0 && secondBestInsert>0);
assert(!valid || bestOverlap>0) : bestOverlap+", "+bestInsert;
}
@Override
public String toString(){
return Tools.format("%d\t%d\t%d\t%d\t%d\t%d\t%.4f\t%.4f\t%.4f\t%.4f\t%.4f\t%.4f\t%.8f",
correct ? 1 : 0, minOverlap, bestOverlap, bestBadInt, secondBestOverlap, secondBestBadInt,
expectedErrors1+expectedErrors2, bestExpected, bestRatio, bestBad, secondBestRatio, secondBestBad, probability);
}
int trueInsert;
int minOverlap;
float expectedErrors1;
float expectedErrors2;
float bestExpected;
float probability;
int bestInsert;
int bestOverlap;
float bestRatio;
float bestBad;
int bestBadInt;
int secondBestInsert;
int secondBestOverlap;
float secondBestRatio;
float secondBestBad;
int secondBestBadInt;
boolean correct;
boolean valid=false;
String line;
}
/*--------------------------------------------------------------*/
private String in1=null;
private String out1=null;
private final FileFormat ffin1;
private final FileFormat ffout1;
/*--------------------------------------------------------------*/
private long maxReads=-1;
/*--------------------------------------------------------------*/
private java.io.PrintStream outstream=System.err;
public static boolean verbose=false;
}
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