1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218
|
package pacbio;
import java.util.ArrayList;
import dna.Data;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import fileIO.TextStreamWriter;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Timer;
import stream.ConcurrentReadInputStream;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ByteBuilder;
import structures.ListNum;
import tracker.ReadStats;
/**
* @author Brian Bushnell
* @date Nov 15, 2012
*
*/
public class PartitionReads {
public static void main(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, new Object() { }.getClass().getEnclosingClass(), false);
args=pp.args;
//outstream=pp.outstream;
}
Timer t=new Timer();
boolean verbose=false;
int ziplevel=-1;
String in1=null;
String in2=null;
long maxReads=-1;
String outname1=null;
String outname2=null;
for(int i=0; i<args.length; i++){
final String arg=args[i];
final String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(Parser.parseCommonStatic(arg, a, b)){
//do nothing
}else if(Parser.parseZip(arg, a, b)){
//do nothing
}else if(Parser.parseQuality(arg, a, b)){
//do nothing
}else if(Parser.parseFasta(arg, a, b)){
//do nothing
}else if(a.equals("path") || a.equals("root") || a.equals("tempdir")){
Data.setPath(b);
}else if(a.equals("fasta") || a.equals("in") || a.equals("input") || a.equals("in1") || a.equals("input1")){
in1=b;
if(b.indexOf('#')>-1){
in1=b.replace("#", "1");
in2=b.replace("#", "2");
}
}else if(a.equals("in2") || a.equals("input2")){
in2=b;
}else if(a.startsWith("partition")){
partitions=Integer.parseInt(b);
}else if(a.equals("append") || a.equals("app")){
append=ReadStats.append=Parse.parseBoolean(b);
}else if(a.equals("overwrite") || a.equals("ow")){
overwrite=Parse.parseBoolean(b);
System.out.println("Set overwrite to "+overwrite);
}else if(a.equals("reads") || a.equals("maxreads")){
maxReads=Parse.parseKMG(b);
}else if(a.equals("out") || a.equals("out1")){
if(b==null || b.equalsIgnoreCase("null") || b.equalsIgnoreCase("none") || split.length==1){
System.out.println("No output file.");
outname1=null;
}else{
outname1=b;
assert(!outname1.equalsIgnoreCase(outname2));
}
}else if(a.equals("out2")){
if(b==null || b.equalsIgnoreCase("null") || b.equalsIgnoreCase("none") || split.length==1){
outname2=null;
}else{
outname2=b;
assert(!outname2.equalsIgnoreCase(outname1));
}
}else if(a.startsWith("verbose")){
verbose=Parse.parseBoolean(b);
}else{
throw new RuntimeException("Unknown parameter: "+args[i]);
}
}
{//Process parser fields
Parser.processQuality();
}
assert(FastaReadInputStream.settingsOK());
assert(outname1==null || outname1.indexOf('#')>=0 || partitions<2);
assert(outname2==null || outname2.indexOf('#')>=0 || partitions<2);
assert(outname1==null || !outname1.equalsIgnoreCase(outname2));
if(in1==null){throw new RuntimeException("Please specify input file.");}
final ConcurrentReadInputStream cris;
{
FileFormat ff1=FileFormat.testInput(in1, FileFormat.FASTQ, null, true, true);
FileFormat ff2=FileFormat.testInput(in2, FileFormat.FASTQ, null, true, true);
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ff1, ff2);
if(verbose){System.err.println("Started cris");}
// cris.start(); //4567
// th.start();
}
TextStreamWriter[] tsw1=new TextStreamWriter[partitions];
TextStreamWriter[] tsw2=new TextStreamWriter[partitions];
FileFormat ff=FileFormat.testOutput(outname1, FileFormat.FASTQ, null, true, overwrite, append, false);
fastq=ff.fastq();
fasta=ff.fasta();
bread=ff.bread();
for(int i=0; i<partitions; i++){
tsw1[i]=new TextStreamWriter(outname1.replaceFirst("#", ""+i), overwrite, false, true);
if(outname2!=null){
tsw2[i]=new TextStreamWriter(outname2.replaceFirst("#", ""+i), overwrite, false, true);
}
}
long reads=process(tsw1, tsw2, cris);
t.stop();
System.out.println("Reads: \t"+reads);
System.out.println("Time: \t"+t);
}
public static long process(TextStreamWriter[] tsw1, TextStreamWriter[] tsw2, ConcurrentReadInputStream cris){
for(TextStreamWriter tsw : tsw1){if(tsw!=null){tsw.start();}}
for(TextStreamWriter tsw : tsw2){if(tsw!=null){tsw.start();}}
cris.start(); //4567
ListNum<Read> ln=cris.nextList();
ArrayList<Read> readlist=ln.list;
final boolean paired=cris.paired();
long x=0;
final int div=tsw1.length;
while(!readlist.isEmpty()){
//System.err.println("Got a list of size "+readlist.size());
for(int i=0; i<readlist.size(); i++){
Read r=readlist.get(i);
if(r!=null){
final Read r2=r.mate;
final int mod=(int)(x%div);
ByteBuilder a=null, b=null;
if(fastq){
a=r.toFastq();
if(paired){b=r2.toFastq();}
}else if(fasta){
a=r.toFasta();
if(paired){b=r2.toFasta();}
}else if(bread){
a=r.toText(true);
if(paired){b=(r2==null ? new ByteBuilder(".") : r2.toText(true));}
}else{
throw new RuntimeException("Unsupported output format.");
}
a.append('\n');
tsw1[mod].print(a);
if(paired){
b.append('\n');
if(tsw2[i]!=null){tsw2[i].print(b);}
else{tsw1[i].print(b);}
}
x++;
}
}
cris.returnList(ln.id, readlist.isEmpty());
//System.err.println("Waiting on a list...");
ln=cris.nextList();
readlist=ln.list;
}
//System.err.println("Returning a list... (final)");
assert(readlist.isEmpty());
cris.returnList(ln.id, readlist.isEmpty());
for(TextStreamWriter tsw : tsw1){if(tsw!=null){tsw.poison();}}
for(TextStreamWriter tsw : tsw2){if(tsw!=null){tsw.poison();}}
ReadWrite.closeStream(cris);
return x;
}
/** Permission to overwrite existing files */
public static boolean overwrite=true;
/** Permission to append to existing files */
public static boolean append=false;
public static int partitions=2;
public static boolean fastq=false;
public static boolean fasta=false;
public static boolean bread=false;
}
|