1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563
|
package prok;
import java.io.File;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.HashMap;
import java.util.Map.Entry;
import java.util.concurrent.locks.ReadWriteLock;
import java.util.concurrent.locks.ReentrantReadWriteLock;
import aligner.SingleStateAlignerFlat2;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import structures.ListNum;
import tax.GiToTaxid;
import template.Accumulator;
import template.ThreadWaiter;
import tracker.ReadStats;
/**
* Picks one ribosomal (16S) sequence per taxID.
* This is the old version that does not make a consensus per taxID.
* Instead it just picks whatever aligns best to the global consensus.
*
* @author Brian Bushnell
* @date November 19, 2015
*
*/
public class MergeRibo_Fast implements Accumulator<MergeRibo_Fast.ProcessThread> {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
MergeRibo_Fast x=new MergeRibo_Fast(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public MergeRibo_Fast(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
{//Parse the arguments
final Parser parser=parse(args);
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
extin=parser.extin;
out1=parser.out1;
extout=parser.extout;
}
validateParams();
adjustInterleaving(); //Make sure interleaving agrees with number of input and output files
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
//Create output FileFormat objects
ffout1=FileFormat.testOutput(out1, FileFormat.FASTA, extout, true, overwrite, append, ordered);
//Create input FileFormat objects
ffin=new ArrayList<FileFormat>(in.size());
ffalt=FileFormat.testInput(alt, FileFormat.FASTA, extin, true, true);
for(String s : in){
FileFormat ff=FileFormat.testInput(s, FileFormat.FASTA, extin, true, true);
ffin.add(ff);
}
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
//Create a parser object
Parser parser=new Parser();
//Set any necessary Parser defaults here
//parser.foo=bar;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("ordered")){
ordered=Parse.parseBoolean(b);
}else if(a.equals("in")){
Tools.addFiles(b, in);
}else if(a.equals("alt")){
alt=b;
}else if(a.equals("parse_flag_goes_here")){
long fake_variable=Parse.parseKMG(b);
//Set a variable here
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else if(b==null && new File(arg).exists()){
in.add(arg);
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
}
}
assert(!in.isEmpty()) : "No input file.";
return parser;
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in)){
throw new RuntimeException("\nCan't read some input files.\n");
}
// //Ensure that no file was specified multiple times
// if(!Tools.testForDuplicateFiles(true, out1, in.toArray(new String[0]))){
// throw new RuntimeException("\nSome file names were specified multiple times.\n");
// }
}
/** Make sure interleaving agrees with number of input and output files */
private void adjustInterleaving(){
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
assert(FastaReadInputStream.settingsOK());
}
/** Ensure parameter ranges are within bounds and required parameters are set */
private boolean validateParams(){
// assert(minfoo>0 && minfoo<=maxfoo) : minfoo+", "+maxfoo;
// assert(false) : "TODO";
return true;
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
if(process16S){
Read[] data=ProkObject.loadConsensusSequenceType("16S", true, true);
consensus16S=data[0].bases;
}
if(process18S){
Read[] data=ProkObject.loadConsensusSequenceType("18S", true, true);
consensus18S=data[0].bases;
}
//Turn off read validation in the input threads to increase speed
final boolean vic=Read.VALIDATE_IN_CONSTRUCTOR;
Read.VALIDATE_IN_CONSTRUCTOR=Shared.threads()<4;
//Reset counters
readsProcessed=readsOut=0;
basesProcessed=basesOut=0;
for(FileFormat ff : ffin) {
//Create a read input stream
final ConcurrentReadInputStream cris=makeCris(ff);
//Process the reads in separate threads
spawnThreads(cris);
errorState|=ReadWrite.closeStream(cris);
}
//Do anything necessary after processing
if(ffout1!=null){
//Optionally create a read output stream
final ConcurrentReadOutputStream ros=makeCros(false);
long num=0;
for(Entry<Integer, Ribo> e : bestMap.entrySet()){
Read r=e.getValue().r;
readsOut++;
basesOut+=r.length();
ArrayList<Read> list=new ArrayList<Read>(1);
list.add(r);
ros.add(list, num);
num++;
}
//Close the read streams
errorState|=ReadWrite.closeStream(ros);
}
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Reset read validation
Read.VALIDATE_IN_CONSTRUCTOR=vic;
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
outstream.println(Tools.readsBasesOut(readsProcessed, basesProcessed, readsOut, basesOut, 8, false));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
private ConcurrentReadInputStream makeCris(FileFormat ff){
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(maxReads, true, ff, null);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
boolean paired=cris.paired();
assert(!paired) : "This should not be paired input.";
return cris;
}
private ConcurrentReadOutputStream makeCros(boolean pairedInput){
if(ffout1==null){return null;}
//Select output buffer size based on whether it needs to be ordered
final int buff=(ordered ? Tools.mid(16, 128, (Shared.threads()*2)/3) : 8);
final ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(ffout1, null, buff, null, false);
ros.start(); //Start the stream
return ros;
}
/*--------------------------------------------------------------*/
/*---------------- Thread Management ----------------*/
/*--------------------------------------------------------------*/
/** Spawn process threads */
private void spawnThreads(final ConcurrentReadInputStream cris){
//Do anything necessary prior to processing
//Determine how many threads may be used
final int threads=Shared.threads();
//Fill a list with ProcessThreads
ArrayList<ProcessThread> alpt=new ArrayList<ProcessThread>(threads);
for(int i=0; i<threads; i++){
alpt.add(new ProcessThread(cris, i));
}
//Start the threads and wait for them to finish
boolean success=ThreadWaiter.startAndWait(alpt, this);
errorState&=!success;
}
@Override
public final void accumulate(ProcessThread pt){
readsProcessed+=pt.readsProcessedT;
basesProcessed+=pt.basesProcessedT;
errorState|=(!pt.success);
}
@Override
public final boolean success(){return !errorState;}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/** This class is static to prevent accidental writing to shared variables.
* It is safe to remove the static modifier. */
class ProcessThread extends Thread {
//Constructor
ProcessThread(final ConcurrentReadInputStream cris_, final int tid_){
cris=cris_;
tid=tid_;
}
//Called by start()
@Override
public void run(){
//Do anything necessary prior to processing
//Process the reads
processInner();
//Do anything necessary after processing
//Indicate successful exit status
success=true;
}
/** Iterate through the reads */
void processInner(){
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//Check to ensure pairing is as expected
if(ln!=null && !ln.isEmpty()){
Read r=ln.get(0);
// assert(ffin1.samOrBam() || (r.mate!=null)==cris.paired()); //Disabled due to non-static access
}
//As long as there is a nonempty read list...
while(ln!=null && ln.size()>0){
// if(verbose){outstream.println("Fetched "+reads.size()+" reads.");} //Disabled due to non-static access
processList(ln);
//Notify the input stream that the list was used
cris.returnList(ln);
// if(verbose){outstream.println("Returned a list.");} //Disabled due to non-static access
//Fetch a new list
ln=cris.nextList();
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
void processList(ListNum<Read> ln){
//Grab the actual read list from the ListNum
final ArrayList<Read> reads=ln.list;
//Loop through each read in the list
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
//Validate reads in worker threads
if(!r1.validated()){r1.validate(true);}
//Track the initial length for statistics
final int initialLength1=r1.length();
//Increment counters
readsProcessedT++;
basesProcessedT+=initialLength1;
boolean keep=processRead(r1);
}
}
/**
* Process a read or a read pair.
* @return True if the reads should be kept, false if they should be discarded.
*/
boolean processRead(final Read r){
Integer key=GiToTaxid.parseTaxidNumber(r.id, '|');
if(key==null || key==-1){return false;}
float id=align(r);
float product=id*r.length();
Ribo ribo=new Ribo(r, key, id);
synchronized(bestMap){
Ribo old=bestMap.get(key);
if(old==null || old.product<product){
bestMap.put(key, ribo);
return true;
}
}
return false;
}
float align(Read r){
float a=(process16S ? align(r.bases, consensus16S) : 0);
float b=(process18S ? align(r.bases, consensus18S) : 0);
return Tools.max(a, b);
}
float align(byte[] query, byte[] ref){
int a=0, b=ref.length-1;
int[] max=ssa.fillUnlimited(query, ref, a, b, -9999);
if(max==null){return 0;}
final int rows=max[0];
final int maxCol=max[1];
final int maxState=max[2];
final float id=ssa.tracebackIdentity(query, ref, a, b, rows, maxCol, maxState, null);
return id;
}
SingleStateAlignerFlat2 ssa=new SingleStateAlignerFlat2();
/** Number of reads processed by this thread */
protected long readsProcessedT=0;
/** Number of bases processed by this thread */
protected long basesProcessedT=0;
/** True only if this thread has completed successfully */
boolean success=false;
/** Shared input stream */
private final ConcurrentReadInputStream cris;
/** Thread ID */
final int tid;
}
private static class Ribo implements Comparable<Ribo>{
Ribo(Read r_, int tid_, float identity_){
r=r_;
tid=tid_;
identity=identity_;
product=r.length()*identity;
}
@Override
public int compareTo(Ribo o) {
if(o.product>product){return -1;}
else if(o.product<product){return 1;}
else if(o.r.length()>r.length()){return -1;}
else if(o.r.length()<r.length()){return 1;}
return 0;
}
Read r;
int tid;
float identity;
float product;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file path */
private ArrayList<String> in=new ArrayList<String>();
/** Alternate input file path */
private String alt=null;
/** Primary output file path */
private String out1=null;
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
HashMap<Integer, Ribo> bestMap=new HashMap<Integer, Ribo>(10000000);
HashMap<Integer, ArrayList<Ribo>> listMap=new HashMap<Integer, ArrayList<Ribo>>(10000000);
static byte[] consensus16S;
byte[] consensus18S;
/*--------------------------------------------------------------*/
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
/** Number of reads retained */
protected long readsOut=0;
/** Number of bases retained */
protected long basesOut=0;
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
private boolean process16S=true;
private boolean process18S=false;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file */
private final ArrayList<FileFormat> ffin;
private final FileFormat ffalt;
/** Primary output file */
private final FileFormat ffout1;
@Override
public final ReadWriteLock rwlock() {return rwlock;}
private final ReadWriteLock rwlock=new ReentrantReadWriteLock();
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
/** Reads are output in input order */
private boolean ordered=false;
}
|