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package sketch;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.Arrays;
import fileIO.ByteFile;
import fileIO.ByteStreamWriter;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import structures.ByteBuilder;
import tax.TaxTree;
/**
* @author Brian Bushnell
* @date May 9, 2016
*
*/
public class AddSSU {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
//Start a timer immediately upon code entrance.
Timer t=new Timer();
//Create an instance of this class
AddSSU x=new AddSSU(args);
//Run the object
x.process(t);
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public AddSSU(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, /*getClass()*/null, false);
args=pp.args;
outstream=pp.outstream;
}
//Set shared static variables prior to parsing
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
{//Parse the arguments
final Parser parser=parse(args);
overwrite=parser.overwrite;
append=parser.append;
in1=parser.in1;
out1=parser.out1;
}
fixExtensions(); //Add or remove .gz or .bz2 as needed
checkFileExistence(); //Ensure files can be read and written
checkStatics(); //Adjust file-related static fields as needed for this program
ffout1=FileFormat.testOutput(out1, FileFormat.SKETCH, null, true, overwrite, append, false);
ffin1=FileFormat.testInput(in1, FileFormat.SKETCH, null, true, false);
if(verbose){
System.err.println("Set r16SFile="+r16SFile);
System.err.println("Set r18SFile="+r18SFile);
}
tree=(treeFile!=null && (preferSSUMapEuks || preferSSUMapProks || clear16SEuks || clear18SEuks ||
clear16SProks || clear18SProks || useSSUMapOnlyEuks || useSSUMapOnlyProks) ? TaxTree.loadTaxTree(treeFile, outstream, false, false) : null);
if(preferSSUMapEuks || preferSSUMapProks || clear16SEuks || clear18SEuks || clear16SProks || clear18SProks || useSSUMapOnlyEuks || useSSUMapOnlyProks){
assert(tree!=null) : "preferSSUMapForEuks, clear16SEuks, and clear18SEuks require a TaxTree.";
}
}
/*--------------------------------------------------------------*/
/*---------------- Initialization Helpers ----------------*/
/*--------------------------------------------------------------*/
/** Parse arguments from the command line */
private Parser parse(String[] args){
Parser parser=new Parser();
for(int i=0; i<args.length; i++){
String arg=args[i];
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(b!=null && b.equalsIgnoreCase("null")){b=null;}
if(a.equalsIgnoreCase("16S") || a.equalsIgnoreCase("16Sfile")){
r16SFile=b;
}else if(a.equalsIgnoreCase("18S") || a.equalsIgnoreCase("18Sfile")){
r18SFile=b;
}else if(a.equalsIgnoreCase("tree") || a.equalsIgnoreCase("treefile")){
treeFile=b;
}else if(a.equals("lines")){
maxLines=Long.parseLong(b);
if(maxLines<0){maxLines=Long.MAX_VALUE;}
}else if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
// ByteFile1.verbose=verbose;
// ByteFile2.verbose=verbose;
// ReadWrite.verbose=verbose;
}
else if(a.equalsIgnoreCase("preferSSUMap")){
preferSSUMap=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("preferSSUMapForEuks") || a.equalsIgnoreCase("preferSSUMapEuks")){
preferSSUMapEuks=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("useSSUMapOnly")){
useSSUMapOnly=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("useSSUMapOnlyEuks") || a.equalsIgnoreCase("SSUMapOnlyEuks")){
useSSUMapOnlyEuks=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("useSSUMapOnlyProks") || a.equalsIgnoreCase("SSUMapOnlyProks")){
useSSUMapOnlyProks=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("preferSSUMapForProks") || a.equalsIgnoreCase("preferSSUMapProks")){
preferSSUMapProks=Parse.parseBoolean(b);
}
else if(a.equalsIgnoreCase("clearAll")){
clear16S=clear18S=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("clear16S")){
clear16S=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("clear18S")){
clear18S=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("clear16SEuks")){
clear16SEuks=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("clear18SEuks")){
clear18SEuks=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("clear16SProks")){
clear16SProks=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("clear18SProks")){
clear18SProks=Parse.parseBoolean(b);
}
else if(parser.parse(arg, a, b)){
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
if("auto".equalsIgnoreCase(r16SFile)){r16SFile=TaxTree.default16SFile();}
if("auto".equalsIgnoreCase(r18SFile)){r18SFile=TaxTree.default18SFile();}
SSUMap.r16SFile=r16SFile;
SSUMap.r18SFile=r18SFile;
return parser;
}
/** Add or remove .gz or .bz2 as needed */
private void fixExtensions(){
in1=Tools.fixExtension(in1);
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
}
/** Ensure files can be read and written */
private void checkFileExistence(){
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1)){
outstream.println((out1==null)+", "+out1);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output file "+out1+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1, r16SFile, r18SFile)){
throw new RuntimeException("\nCan't read some input files.\n");
}
assert(in1!=null) : "Input sketch file is required";
assert(r16SFile!=null || r18SFile!=null) : "Input SSU file is required";
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, out1, r16SFile, r18SFile)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
}
/** Adjust file-related static fields as needed for this program */
private static void checkStatics(){
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
// if(!ByteFile.FORCE_MODE_BF2){
// ByteFile.FORCE_MODE_BF2=false;
// ByteFile.FORCE_MODE_BF1=true;
// }
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
void process(Timer t){
ByteFile bf=ByteFile.makeByteFile(ffin1);
ByteStreamWriter bsw=makeBSW(ffout1);
processInner(bf, bsw);
errorState|=bf.close();
if(bsw!=null){errorState|=bsw.poisonAndWait();}
t.stop();
outstream.println(Tools.timeLinesBytesProcessed(t, linesProcessed, bytesProcessed, 8));
outstream.println(Tools.linesBytesOut(linesProcessed, bytesProcessed, linesOut, bytesOut, 8, true));
outstream.println();
outstream.println(Tools.number("Sketches:", sketchCount, 8));
outstream.println(Tools.number("16S In:", r16Sin, 8));
outstream.println(Tools.number("18S In:", r18Sin, 8));
outstream.println(Tools.number("16S Added:", r16SfromMap, 8));
outstream.println(Tools.number("18S Added:", r18SfromMap, 8));
outstream.println(Tools.numberPercent("16S Out:", r16Sout, r16Sout*100.0/sketchCount, 2, 8));
outstream.println(Tools.numberPercent("18S Out:", r18Sout, r18Sout*100.0/sketchCount, 2, 8));
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
private static ByteStreamWriter makeBSW(FileFormat ff){
if(ff==null){return null;}
ByteStreamWriter bsw=new ByteStreamWriter(ff);
bsw.start();
return bsw;
}
// private void processInner_old(ByteFile bf, ByteStreamWriter bsw){
// SSUMap.load(outstream);
//
// if(verbose){
// System.err.println("Loaded SSUMap; |16S|="+SSUMap.r16SCount()+", |18S|="+SSUMap.r18SCount());
// }
//
// byte[] line=bf.nextLine();
//// ByteBuilder bb=new ByteBuilder();
//
// final byte[] ssuBytes="SSU:".getBytes();
// final byte[] r16SBytes="16S:".getBytes();
// final byte[] r18SBytes="18S:".getBytes();
//
// while(line!=null){
// if(line.length>0){
// if(maxLines>0 && linesProcessed>=maxLines){break;}
// linesProcessed++;
// bytesProcessed+=(line.length+1);
//
// final boolean header=(line[0]=='#');
//
// linesOut++;
// bytesOut+=(line.length+1);
//
// if(header){
// if(Tools.startsWith(line, "#SZ:")){
// sketchCount++;
//
// bsw.print(line);
//
// final int tid=parseTaxID(line);
// final boolean has16S=Tools.contains(line, ssuBytes, 0) || Tools.contains(line, r16SBytes, 0);
// final boolean has18S=Tools.contains(line, r18SBytes, 0);
//
// if(verbose){
// System.err.println("For line "+new String(line)+":");
// System.err.println("tid="+tid+", has16S="+has16S+", has18S="+has18S);
// }
//
// if(tid>0){
// final byte[] r16S=has16S ? null : SSUMap.r16SMap.get(tid);
// final byte[] r18S=has18S ? null : SSUMap.r18SMap.get(tid);
// if(r16S!=null){bsw.print("\t16S:").print(r16S.length); ssuOut++;}
// if(r18S!=null){bsw.print("\t18S:").print(r18S.length); ssuOut++;}
// if(r16S!=null){bsw.print("\n#16S:").print(r16S);}
// if(r18S!=null){bsw.print("\n#18S:").print(r18S);}
//
// if(verbose){System.err.println("Found 16S: "+(r16S!=null)+"; found 18S: "+(r18S!=null));}
// }
// bsw.println();
// }else if(Tools.startsWith(line, "#16S:") || Tools.startsWith(line, "#18S:") || Tools.startsWith(line, "#SSU:")){
// bsw.println(line);
// ssuIn++;
// ssuOut++;
// }else{
// assert(Tools.startsWith(line, "##")) : new String(line);
// bsw.println(line);
// }
// }else{
// bsw.println(line);
// }
// }
// line=bf.nextLine();
// }
// }
private void processInner(ByteFile bf, ByteStreamWriter bsw){
SSUMap.load(outstream);
if(verbose){
System.err.println("Loaded SSUMap; |16S|="+SSUMap.r16SCount()+", |18S|="+SSUMap.r18SCount());
}
byte[] line=bf.nextLine();
// ByteBuilder bb=new ByteBuilder();
// final byte[] ssuBytes="SSU:".getBytes();
// final byte[] r16SBytes="16S:".getBytes();
// final byte[] r18SBytes="18S:".getBytes();
SketchHeader header=null;
while(line!=null){
if(line.length>0){
if(maxLines>0 && linesProcessed>=maxLines){break;}
linesProcessed++;
bytesProcessed+=(line.length+1);
final boolean isHeader=(line[0]=='#');
if(isHeader){
if(Tools.startsWith(line, "#SZ:")){
assert(header==null) : "\nReplacing this:\n"+header.toBytes()+"\nWith this:\n"+new String(line)+"\n";
header=new SketchHeader(line);
sketchCount++;
}else if(Tools.startsWith(line, "##")){
bsw.println(line);
linesOut++;
bytesOut+=(line.length+1);
}else{
header.addLine(line);
}
}else{
if(header!=null){
try {
processHeader(header);
} catch (Throwable e) {
e.printStackTrace();
assert(false) : header.toBytes();
}
r16Sout+=(header.r16S==null ? 0 : 1);
r18Sout+=(header.r18S==null ? 0 : 1);
linesOut+=1+(header.r16S==null ? 0 : 1)+(header.r18S==null ? 0 : 1);
ByteBuilder bb=header.toBytes();
bytesOut+=(bb.length+1);
bsw.println(bb);
header=null;
}
bsw.println(line);
linesOut++;
bytesOut+=(line.length+1);
}
}
line=bf.nextLine();
}
}
void processHeader(SketchHeader header){
if(verbose){System.err.println("Processing tid "+header.tid+":\n"+header.toBytes()+"\n");}
final boolean euk=(tree!=null && header.tid>0 && header.tid<SketchObject.minFakeID) ? tree.isEukaryote(header.tid) : false;
final boolean prok=(tree!=null && header.tid>0 && header.tid<SketchObject.minFakeID) ? tree.isProkaryote(header.tid) : false;
if(useSSUMapOnly || (useSSUMapOnlyEuks && euk) || (useSSUMapOnlyProks && prok)){header.r16S=header.r18S=null;}
if(header.tid>0){
final boolean preferMap=(preferSSUMap || (preferSSUMapEuks && euk) || (preferSSUMapProks && prok));
byte[] r16S=(SSUMap.r16SMap==null ? null : SSUMap.r16SMap.get(header.tid));
byte[] r18S=(SSUMap.r18SMap==null ? null : SSUMap.r18SMap.get(header.tid));
if(r16S!=null && (preferMap || header.r16S==null)){
header.r16S=r16S;
r16SfromMap++;
}
if(r18S!=null && (preferMap || header.r18S==null)){
header.r18S=r18S;
r18SfromMap++;
}
}
if(clear16S || (clear16SEuks && euk) || (clear16SProks && prok)){header.r16S=null;}
if(clear18S || (clear18SEuks && euk) || (clear18SProks && prok)){header.r18S=null;}
}
int parseTaxID(byte[] line){
String[] split=Tools.tabPattern.split(new String(line));
for(String s : split){
if(s.startsWith("ID:") || s.startsWith("TAXID:")){
final int colon=s.indexOf(':');
final String sub=s.substring(colon+1);
return Integer.parseInt(sub);
}
}
return -1;
}
/*--------------------------------------------------------------*/
//A very limited parser
private class SketchHeader {
SketchHeader(byte[] line){
this(new String(line, 1, line.length-1));
}
SketchHeader(String line){
if(line.charAt(0)=='#'){line=line.substring(1);}
assert(line.startsWith("SZ:"));
String[] split=Tools.tabPattern.split(line);
fields=new ArrayList<String>(line.length()+2);
int tid_=-1;
for(String s : split){
if(s.startsWith("16S:") || s.startsWith("18S:") || s.startsWith("SSU:")){
//do nothing
}else{
if(s.startsWith("ID:") || s.startsWith("TAXID:")){
final int colon=s.indexOf(':');
final String sub=s.substring(colon+1);
tid_=Integer.parseInt(sub);
}
fields.add(s);
}
}
tid=tid_;
}
void addLine(byte[] line){
assert(line[0]=='#');
assert(line[1]=='1' || line[1]=='S') : new String(line);
if(Tools.startsWith(line, "#16S:") || Tools.startsWith(line, "#SSU:")){
assert(r16S==null);
r16S=Arrays.copyOfRange(line, 5, line.length);
r16Sin++;
}else if(Tools.startsWith(line, "#18S:")){
assert(r18S==null);
r18S=Arrays.copyOfRange(line, 5, line.length);
r18Sin++;
}else{
assert(false) : new String(line);
}
}
ByteBuilder toBytes(){
ByteBuilder bb=new ByteBuilder(1000);
bb.append('#');
for(int i=0; i<fields.size(); i++){
if(i>0){bb.tab();}
bb.append(fields.get(i));
}
if(r16S!=null){bb.tab().append("16S:").append(r16S.length);}
if(r18S!=null){bb.tab().append("18S:").append(r18S.length);}
if(r16S!=null){bb.nl().append("#16S:").append(r16S);}
if(r18S!=null){bb.nl().append("#18S:").append(r18S);}
return bb;
}
final int tid;
ArrayList<String> fields;
byte[] r16S;
byte[] r18S;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
private String in1=null;
private String out1=null;
private String r16SFile="auto";
private String r18SFile="auto";
private String treeFile="auto";
boolean preferSSUMap=false;
boolean preferSSUMapEuks=false;
boolean preferSSUMapProks=false;
boolean useSSUMapOnly=false;
boolean useSSUMapOnlyEuks=false;
boolean useSSUMapOnlyProks=false;
boolean clear16S=false;
boolean clear18S=false;
boolean clear16SEuks=false;
boolean clear18SEuks=false;
boolean clear16SProks=false;
boolean clear18SProks=false;
/*--------------------------------------------------------------*/
private long linesProcessed=0;
private long linesOut=0;
private long bytesProcessed=0;
private long bytesOut=0;
private long sketchCount=0;
private long r16Sin=0;
private long r16Sout=0;
private long r16SfromMap=0;
private long r18Sin=0;
private long r18Sout=0;
private long r18SfromMap=0;
private long maxLines=Long.MAX_VALUE;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
private final FileFormat ffin1;
private final FileFormat ffout1;
private final TaxTree tree;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
private PrintStream outstream=System.err;
public static boolean verbose=false;
public boolean errorState=false;
private boolean overwrite=true;
private boolean append=false;
}
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