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package sort;
import java.io.File;
import java.io.IOException;
import java.io.PrintStream;
import java.util.ArrayList;
import java.util.PriorityQueue;
import java.util.concurrent.atomic.AtomicLong;
import fileIO.ByteFile;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.KillSwitch;
import shared.Parse;
import shared.Parser;
import shared.PreParser;
import shared.Shared;
import shared.Timer;
import shared.Tools;
import stream.ConcurrentReadInputStream;
import stream.ConcurrentReadOutputStream;
import stream.CrisContainer;
import stream.FASTQ;
import stream.FastaReadInputStream;
import stream.Read;
import stream.SamLine;
import structures.ListNum;
import tax.AccessionToTaxid;
import tax.GiToTaxid;
import tax.TaxTree;
import tracker.ReadStats;
import var2.ScafMap;
/**
* Sorts reads by name, potentially from multiple input files.
*
* @author Brian Bushnell
* @date September 21, 2016
*
*/
public class SortByName {
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/**
* Code entrance from the command line.
* @param args Command line arguments
*/
public static void main(String[] args){
Timer t=new Timer();
final boolean oldFI=FASTQ.FORCE_INTERLEAVED, oldTI=FASTQ.TEST_INTERLEAVED;
SortByName x=new SortByName(args);
x.process(t);
FASTQ.FORCE_INTERLEAVED=oldFI;
FASTQ.TEST_INTERLEAVED=oldTI;
//Close the print stream if it was redirected
Shared.closeStream(x.outstream);
}
/**
* Constructor.
* @param args Command line arguments
*/
public SortByName(String[] args){
{//Preparse block for help, config files, and outstream
PreParser pp=new PreParser(args, getClass(), false);
args=pp.args;
outstream=pp.outstream;
}
boolean setInterleaved=false; //Whether interleaved was explicitly set.
//Set shared static variables
Shared.capBuffers(4);
Shared.setBufferData(1000000);
ReadWrite.USE_PIGZ=ReadWrite.USE_UNPIGZ=true;
ReadWrite.setZipThreads(Shared.threads());
FASTQ.TEST_INTERLEAVED=true; //TEST_INTERLEAVED must explicitly be disabled with int=f to sort corrupt files.
FASTQ.FORCE_INTERLEAVED=false;
//Create a parser object
Parser parser=new Parser();
boolean ascending=true;
//Parse each argument
for(int i=0; i<args.length; i++){
String arg=args[i];
//Break arguments into their constituent parts, in the form of "a=b"
String[] split=arg.split("=");
String a=split[0].toLowerCase();
String b=split.length>1 ? split[1] : null;
if(a.equals("verbose")){
verbose=Parse.parseBoolean(b);
}else if(a.equals("verbose2")){
assert(false) : "Verbose2 is disabled.";
// verbose2=Parse.parseBoolean(b);
}else if(a.equals("delete")){
delete=Parse.parseBoolean(b);
}else if(a.equalsIgnoreCase("deleteearly")){
deleteEarly=Parse.parseBoolean(b);
}else if(a.equals("allowtemp") || a.equals("usetemp")){
allowTempFiles=Parse.parseBoolean(b);
}else if(a.equals("memmult") || a.equals("mult")){
memBlockMult=(float) Double.parseDouble(b);
}else if(a.equals("memlimit")){
memTotalMult=(float) Double.parseDouble(b);
}else if(a.equals("ascending")){
ascending=Parse.parseBoolean(b);
}else if(a.equals("descending")){
ascending=!Parse.parseBoolean(b);
}else if(a.equals("length")){
if(Parse.parseBoolean(b)){
comparator=ReadLengthComparator.comparator;
}
}else if(a.equals("name")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorName.comparator;
}
}else if(a.equals("quality")){
if(Parse.parseBoolean(b)){
comparator=ReadQualityComparator.comparator;
}
}else if(a.equals("position")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorPosition.comparator;
}
}else if(a.equals("list") || a.equals("names")){
comparator=new ReadComparatorList(b);
}else if(a.equals("random") || a.equals("shuffle")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorRandom.comparator;
}
}else if(a.equals("taxa")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorTaxa.comparator;
}
}else if(a.equals("topo") || a.equals("topological") || a.equals("alphabetic") || a.equals("sequence") || a.equals("lexographic")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorTopological.comparator;
}
}else if(a.equals("flowcell")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorFlowcell.comparator;
}
}else if(a.equals("crispr")){
if(Parse.parseBoolean(b)){
ReadComparatorCrispr.loadNet();
comparator=ReadComparatorCrispr.comparator;
}
}else if(a.equalsIgnoreCase("clump") || a.equalsIgnoreCase("clumpify")){
if(Parse.parseBoolean(b)){
comparator=ReadComparatorClump.comparator;
}
}else if(a.equals("table") || a.equals("gi") || a.equals("gitable")){
if(b==null || "ignore".equalsIgnoreCase(b) || "skip".equalsIgnoreCase(b)){
giTableFile=null;
TaxTree.CRASH_IF_NO_GI_TABLE=false;
}else{giTableFile=b;}
}else if(a.equals("accession")){
accessionFile=b;
}else if(a.equals("tree") || a.equals("taxtree")){
taxTreeFile=b;
}else if(a.equals("maxfiles") || a.equals("files")){
maxFiles=Integer.parseInt(b);
assert(maxFiles>1 && maxFiles<=10000000) : "Invalid value for maxfiles: "+maxFiles+"; range is 2-10000000";
setMaxFiles=true;
}else if(a.equals("genkmer")){
genKmer=Parse.parseBoolean(b);
}else if(a.equals("parse_flag_goes_here")){
//Set a variable here
}else if(parser.parse(arg, a, b)){//Parse standard flags in the parser
//do nothing
}else{
outstream.println("Unknown parameter "+args[i]);
assert(false) : "Unknown parameter "+args[i];
// throw new RuntimeException("Unknown parameter "+args[i]);
}
}
if("auto".equalsIgnoreCase(taxTreeFile)){taxTreeFile=TaxTree.defaultTreeFile();}
if("auto".equalsIgnoreCase(giTableFile)){giTableFile=TaxTree.defaultTableFile();}
if("auto".equalsIgnoreCase(accessionFile)){accessionFile=TaxTree.defaultAccessionFile();}
if(comparator==ReadComparatorTopological.comparator && genKmer){
comparator=ReadComparatorTopological5Bit.comparator;
}else{
genKmer=false;
}
clump=(comparator==ReadComparatorClump.comparator);
comparator.setAscending(ascending);
SamLine.SET_FROM_OK=true;
if(comparator==ReadComparatorRandom.comparator){
ListNum.setDeterministicRandomSeed(-1);
ListNum.setDeterministicRandom(true);
}
{//Process parser fields
Parser.processQuality();
maxReads=parser.maxReads;
overwrite=ReadStats.overwrite=parser.overwrite;
append=ReadStats.append=parser.append;
setInterleaved=parser.setInterleaved;
in1=parser.in1;
in2=parser.in2;
qfin1=parser.qfin1;
qfin2=parser.qfin2;
out1=parser.out1;
out2=parser.out2;
extin=parser.extin;
extout=parser.extout;
minlen=parser.minReadLength;
}
// assert(false) : setInterleaved+", "+FASTQ.FORCE_INTERLEAVED+", "+FASTQ.TEST_INTERLEAVED;
// assert(!FASTQ.FORCE_INTERLEAVED) : setInterleaved+", "+FASTQ.FORCE_INTERLEAVED+", "+FASTQ.TEST_INTERLEAVED;
// assert(!FASTQ.TEST_INTERLEAVED) : setInterleaved+", "+FASTQ.FORCE_INTERLEAVED+", "+FASTQ.TEST_INTERLEAVED;
//Do input file # replacement
if(in1!=null && in2==null && in1.indexOf('#')>-1 && !new File(in1).exists()){
in2=in1.replace("#", "2");
in1=in1.replace("#", "1");
}
//Do output file # replacement
if(out1!=null && out2==null && out1.indexOf('#')>-1){
out2=out1.replace("#", "2");
out1=out1.replace("#", "1");
}
//Adjust interleaved detection based on the number of input files
if(in2!=null){
if(FASTQ.FORCE_INTERLEAVED){outstream.println("Reset INTERLEAVED to false because paired input files were specified.");}
FASTQ.FORCE_INTERLEAVED=FASTQ.TEST_INTERLEAVED=false;
}
assert(FastaReadInputStream.settingsOK());
//Ensure there is an input file
if(in1==null){throw new RuntimeException("Error - at least one input file is required.");}
//Adjust the number of threads for input file reading
if(!ByteFile.FORCE_MODE_BF1 && !ByteFile.FORCE_MODE_BF2 && Shared.threads()>2){
ByteFile.FORCE_MODE_BF2=true;
}
//Ensure out2 is not set without out1
if(out1==null && out2!=null){throw new RuntimeException("Error - cannot define out2 without defining out1.");}
//Adjust interleaved settings based on number of output files
if(!setInterleaved){
if(in2==null && out2!=null){
FASTQ.FORCE_INTERLEAVED=true;
FASTQ.TEST_INTERLEAVED=false;
}else if(in2!=null){
FASTQ.FORCE_INTERLEAVED=false;
FASTQ.TEST_INTERLEAVED=false;
}
}
//Ensure output files can be written
if(!Tools.testOutputFiles(overwrite, append, false, out1, out2)){
outstream.println((out1==null)+", "+(out2==null)+", "+out1+", "+out2);
throw new RuntimeException("\n\noverwrite="+overwrite+"; Can't write to output files "+out1+", "+out2+"\n");
}
//Ensure input files can be read
if(!Tools.testInputFiles(false, true, in1, in2)){
throw new RuntimeException("\nCan't read some input files.\n");
}
//Ensure that no file was specified multiple times
if(!Tools.testForDuplicateFiles(true, in1, in2, out1, out2)){
throw new RuntimeException("\nSome file names were specified multiple times.\n");
}
//Create input FileFormat objects
ffin1=FileFormat.testInput(in1, FileFormat.FASTQ, extin, true, true);
ffin2=FileFormat.testInput(in2, FileFormat.FASTQ, extin, true, true);
//Create output FileFormat objects
ffout1=FileFormat.testOutput(out1, FileFormat.FASTQ, extout, true, overwrite, append, false);
ffout2=FileFormat.testOutput(out2, FileFormat.FASTQ, extout, true, overwrite, append, false);
tempExt=Tools.getTempExt(ffin1, ffout1, extout);
if(comparator==ReadComparatorPosition.comparator){
if(ReadComparatorPosition.scafMap==null){
ReadComparatorPosition.scafMap=ScafMap.loadSamHeader(in1);
}
}
if((comparator==ReadComparatorTaxa.comparator)){
if(giTableFile!=null){
outstream.println("Loading gi table.");
GiToTaxid.initialize(giTableFile);
}
if(accessionFile!=null){
outstream.println("Loading accession table.");
AccessionToTaxid.load(accessionFile);
}
if(taxTreeFile!=null){
ReadComparatorTaxa.tree=TaxTree.loadTaxTree(taxTreeFile, outstream, true, false);
assert(ReadComparatorTaxa.tree.nameMap()!=null);
}else{
throw new RuntimeException("No tree specified.");
}
}
qualityObserved=!ffin1.fasta();
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
/** Create read streams and process all data */
void process(Timer t){
//Create a read input stream
final ConcurrentReadInputStream cris;
{
useSharedHeader=(ffin1.samOrBam() && ffout1!=null && ffout1.samOrBam());
cris=ConcurrentReadInputStream.getReadInputStream(maxReads, useSharedHeader, ffin1, ffin2, qfin1, qfin2);
cris.start(); //Start the stream
if(verbose){outstream.println("Started cris");}
}
boolean paired=cris.paired();
if(!ffin1.samOrBam()){outstream.println("Input is being processed as "+(paired ? "paired" : "unpaired"));}
// //Optionally create a read output stream
// final ConcurrentReadOutputStream ros;
// if(ffout1!=null){
// final int buff=4;
//
// if(cris.paired() && out2==null && (in1!=null && !ffin1.samOrBam() && !ffout1.samOrBam())){
// outstream.println("Writing interleaved.");
// }
//
// ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, qfout1, qfout2, buff, null, false);
// ros.start(); //Start the stream
// }else{ros=null;}
//Reset counters
readsProcessed=0;
basesProcessed=0;
//Process the read stream
processInner(cris);
if(verbose){outstream.println("Finished; closing streams.");}
//Write anything that was accumulated by ReadStats
errorState|=ReadStats.writeAll();
//Close the read streams
errorState|=ReadWrite.closeStreams(cris);
//Report timing and results
t.stop();
outstream.println(Tools.timeReadsBasesProcessed(t, readsProcessed, basesProcessed, 8));
//Throw an exception of there was an error in a thread
if(errorState){
throw new RuntimeException(getClass().getName()+" terminated in an error state; the output may be corrupt.");
}
}
/** Iterate through the reads */
public void processInner(final ConcurrentReadInputStream cris){
//Do anything necessary prior to processing
final int ziplevel0=ReadWrite.ZIPLEVEL;
ReadWrite.ZIPLEVEL=Tools.mid(1, ReadWrite.ZIPLEVEL, 2);
ArrayList<Read> storage=new ArrayList<Read>();
final long maxMem=Shared.memAvailable(1);
final long memLimit=(long)(maxMem*memTotalMult);//Crashed once at old 0.75 value
final long currentLimit=(long)(maxMem*memBlockMult);
final int readLimit=2000000000;
long currentMem=0;
long bytesDumped=0;
long dumps=0;
// IntList dumpCount=new IntList();
AtomicLong outstandingMem=new AtomicLong();
if(verbose){outstream.println("maxMem="+maxMem+", memLimit="+memLimit+
", currentMem="+currentMem+", currentLimit="+currentLimit);}
{
//Grab the first ListNum of reads
ListNum<Read> ln=cris.nextList();
//Grab the actual read list from the ListNum
ArrayList<Read> reads=(ln!=null ? ln.list : null);
//Check to ensure pairing is as expected
if(reads!=null && !reads.isEmpty()){
Read r=reads.get(0);
assert((ffin1==null || ffin1.samOrBam()) || (r.mate!=null)==cris.paired());
}
//As long as there is a nonempty read list...
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
if(verbose2){outstream.println("Fetched "+reads.size()+" reads.");}
//Loop through each read in the list
for(int idx=0; idx<reads.size(); idx++){
final Read r1=reads.get(idx);
final Read r2=r1.mate;
//Track the initial length for statistics
final int initialLength1=r1.length();
final int initialLength2=(r1.mateLength());
//Increment counters
readsProcessed+=r1.pairCount();
basesProcessed+=initialLength1+initialLength2;
maxLengthObserved=Tools.max(maxLengthObserved, initialLength1, initialLength2);
if(minlen<1 || initialLength1>=minlen || initialLength2>=minlen){
if(genKmer){ReadComparatorTopological5Bit.genKmer(r1);}
else if(clump){ReadComparatorClump.set(r1);}
currentMem+=r1.countBytes()+(r2==null ? 0 : r2.countBytes());
storage.add(r1);
}
}
if(allowTempFiles && (currentMem>=currentLimit || storage.size()>=readLimit)){
if(verbose){outstream.println("currentMem: "+currentMem+" >= "+currentLimit+", dumping. ");}
outstandingMem.addAndGet(currentMem);
// dumpCount.add(storage.size());
sortAndDump(storage, currentMem, outstandingMem, null, null, false);
storage=new ArrayList<Read>();
bytesDumped+=currentMem;
dumps++;
currentMem=0;
if(verbose){outstream.println("Waiting on memory; outstandingMem="+outstandingMem);}
waitOnMemory(outstandingMem, memLimit);
if(verbose){outstream.println("Done waiting; outstandingMem="+outstandingMem);}
}
//Notify the input stream that the list was used
cris.returnList(ln);
// if(verbose){outstream.println("Returned a list.");}
//Fetch a new list
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
//Notify the input stream that the final list was used
if(ln!=null){
cris.returnList(ln.id, ln.list==null || ln.list.isEmpty());
}
}
outstream.println("Finished reading input.");
outstandingMem.addAndGet(currentMem);
if(dumps==0){
ReadWrite.ZIPLEVEL=ziplevel0;
outstream.println("Sorting.");
if(out1!=null){
sortAndDump(storage, currentMem, outstandingMem, out1, out2, useSharedHeader);
storage=null;
waitOnMemory(outstandingMem, 0);
}else{
Timer t=new Timer();
Shared.sort(storage, comparator);//For timing/testing.
t.stop("Sort Time: \t\t\t");
}
}else{
// dumpCount.add(storage.size());
sortAndDump(storage, currentMem, outstandingMem, null, null, false);
storage=null;
waitOnMemory(outstandingMem, 0);
outstream.println("Merging "+(dumps+1)+" files.");
ReadWrite.ZIPLEVEL=ziplevel0;
mergeAndDump(outTemp, /*dumpCount, */useSharedHeader, false);
}
}
private void waitOnMemory(AtomicLong outstandingMem, long target){
if(outstandingMem.get()>target){
if(verbose){outstream.println("Syncing; outstandingMem="+outstandingMem);}
while(outstandingMem.get()>target){
try {
synchronized(outstandingMem){
outstandingMem.wait(2000);
}
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
}
}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
private final long maxSizeObserved(){//Includes some extra for a margin of error
return Tools.max(maxSizeObservedStatic(), Tools.max(maxLengthObserved, 150)*(qualityObserved ? 2 : 1)+500);
}
final static long maxSizeObservedStatic(){//Includes some extra for a margin of error
return Tools.max(maxLengthObservedStatic, 150)*(qualityObservedStatic ? 2 : 1)+500;
}
private static long mergeMemNeeded(long maxSizeObserved, int inFiles){
return (long)(maxSizeObserved*2*(inFiles+1)*1.6);
}
private static int maxMergeFiles(long maxSizeObserved){
final long memAvailable=Shared.memAvailableAdvanced();
int files=(int)Tools.max(3, memAvailable/Tools.max(maxSizeObserved, 500))-1;
return Tools.min(files, 10000);
}
private static void adjustBuffers(long maxSizeObserved, int inFiles, PrintStream outstream){
if(Shared.numBuffers()<3){return;}
long memNeeded=mergeMemNeeded(maxSizeObserved, inFiles);
long memAvailable=Shared.memAvailableAdvanced();
if(memNeeded>memAvailable*0.8){
outstream.println("Reduced buffers prior to merging due to low memory.");
Shared.capBufferLen(40);
Shared.capBuffers(2);
}
}
/*--------------------------------------------------------------*/
private ArrayList<String> mergeRecursive(final ArrayList<String> inList, boolean allowSubprocess){
assert(maxFiles>1);
ArrayList<String> currentList=inList;
final int oldZL=ReadWrite.ZIPLEVEL;
final boolean deleteAfterMerge=(delete && deleteEarly);
final int limit=Tools.min(mergeFileLimit, maxFiles, maxMergeFiles(maxSizeObserved()));
while(currentList.size()>maxFiles){
ReadWrite.ZIPLEVEL=Tools.min(ReadWrite.ZIPLEVEL, 4);
final int size=currentList.size();
final int groups=(size+limit-1)/limit;
assert(groups>0 && groups<size);
ArrayList<ArrayList<String>> listList=new ArrayList<ArrayList<String>>();
ArrayList<String> outList=new ArrayList<String>();
for(int i=0; i<groups; i++){
listList.add(new ArrayList<String>());
}
for(int i=0; i<size; i++){
listList.get(i%groups).add(currentList.get(i));
}
for(ArrayList<String> subList : listList){
String temp=getTempFile();
FileFormat ffoutTemp=FileFormat.testOutput(temp, FileFormat.FASTQ, null, true, false, false, false);
mergeRecursiveInner(subList, ffoutTemp, null, deleteAfterMerge, allowSubprocess);
outList.add(temp);
}
if(delete && !deleteEarly){//delete them now that this pass is finished
System.err.println("Deleting "+size+" temp files...");
for(ArrayList<String> subList : listList){
for(String fname : subList){new File(fname).delete();}
}
System.err.println(" done.");
}
currentList=outList;
}
ReadWrite.ZIPLEVEL=oldZL;
return currentList;
}
public void mergeRecursiveInner(ArrayList<String> inList, FileFormat ffoutTemp1, FileFormat ffoutTemp2, boolean deleteAfterMerge, boolean allowSubprocess){
errorState|=mergeAndDump(inList, /*null, */ffoutTemp1, ffoutTemp2, deleteAfterMerge, useSharedHeader, allowSubprocess, outstream, maxSizeObserved());
}
private String getTempFile(){
String temp;
File dir=new File(".");//(Shared.tmpdir()==null ? null : new File(Shared.tmpdir()));
if(dir!=null && !dir.exists()){dir.mkdirs();}
try {
temp=File.createTempFile("sort_temp_", tempExt, dir).toString();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
KillSwitch.kill(e.getMessage());
return null;
}
return temp;
}
private boolean mergeAndDump(ArrayList<String> fnames, /*IntList dumpCount, */boolean useHeader, boolean allowSubprocess) {
final long maxSizeObserved=maxSizeObserved();
final int limit=Tools.min(mergeFileLimit, maxMergeFiles(maxSizeObserved));
if(fnames.size()>limit){
outstream.println("Performing recursive merge to reduce open files.");
fnames=mergeRecursive(fnames, allowSubprocess);
}
return mergeAndDump(fnames, /*dumpCount,*/ ffout1, ffout2, delete, useHeader, allowSubprocess, outstream, maxSizeObserved);
}
public static boolean mergeAndDump(ArrayList<String> fnames, FileFormat ffout1, FileFormat ffout2,
boolean deleteAfterMerge, boolean useHeader, boolean allowSubprocess, PrintStream outstream, final long maxSizeObserved) {
final int oldBuffers=Shared.numBuffers();
final int oldBufferLen=Shared.bufferLen();
if(!FileFormat.isFastaFile(fnames.get(0))){
synchronized(SortByName.class){
qualityObservedStatic=true;
}
}
adjustBuffers(maxSizeObserved, fnames.size(), outstream);
System.err.println("Merging "+fnames);
// outstream.println("zl="+ReadWrite.ZIPLEVEL);
// outstream.println("ztd="+ReadWrite.ZIP_THREAD_DIVISOR);
// outstream.println("mzt="+ReadWrite.MAX_ZIP_THREADS);
// outstream.println("pigz="+ReadWrite.USE_PIGZ);
ListNum.setDeterministicRandom(false);
boolean errorState=false;
final ConcurrentReadOutputStream ros;
if(ffout1!=null){
final int buff=1;
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, null, null, buff, null, useHeader);
ros.start(); //Start the stream
}else{ros=null;}
ArrayList<CrisContainer> cclist=new ArrayList<CrisContainer>(fnames.size());
PriorityQueue<CrisContainer> q=new PriorityQueue<CrisContainer>(fnames.size());
for(int i=0; i<fnames.size(); i++){
String fname=fnames.get(i);
// int size=(dumpCount==null ? -1 : dumpCount.get(i));
CrisContainer cc=new CrisContainer(fname, /*size, */comparator, allowSubprocess);
if(cc.peek()!=null){
cclist.add(cc);
q.add(cc);
}
}
//TODO: Use the read counts, stored in dumpCount, to approximately restore random values for shuffling.
mergeAndDump(q, ros, outstream);
if(verbose){
outstream.println("Finished processing "+fnames);
}
for(CrisContainer cc : cclist){
errorState|=cc.close();
}
if(deleteAfterMerge){
for(String fname : fnames){
new File(fname).delete();
}
}
if(ros!=null){errorState|=ReadWrite.closeStream(ros);}
Shared.setBufferLen(oldBufferLen);
Shared.setBuffers(oldBuffers);
return errorState;
}
private static void mergeAndDump(final PriorityQueue<CrisContainer> q, final ConcurrentReadOutputStream ros, PrintStream outstream) {
// for(CrisContainer cc : q){
// assert(!cc.cris().paired()) : FASTQ.TEST_INTERLEAVED+", "+FASTQ.FORCE_INTERLEAVED+
// ", "+cc.cris().fname()+", "+cc.cris().getClass();
// }
long maxLen=0;
final int limit=100000;
ArrayList<Read> buffer=new ArrayList<Read>(2*limit);
while(!q.isEmpty()){
if(verbose2){outstream.println("q size: "+q.size());}
for(int i=0; !q.isEmpty() && (buffer.size()<limit || i<1); i++){//For loop to force it to run at least once
CrisContainer cc=q.poll();
if(verbose2){outstream.println("Polled a cc.");}
ArrayList<Read> list=cc.fetch();
if(verbose2){outstream.println("Grabbed "+list.size()+" reads.");}
buffer.addAll(list);
if(verbose2){outstream.println("Buffer size: "+buffer.size());}
if(cc.hasMore()){
if(verbose2){outstream.println("Returned cc.");}
q.add(cc);
}else{
if(verbose2){outstream.println("Discarded cc.");}
}
}
Shared.sort(buffer, comparator);
if(verbose2){outstream.println("Sorted buffer.");}
// if(repair){
// assert(false) : "TODO";
// }
for(int i=1; i<buffer.size(); i++){
Read a=buffer.get(i-1);
Read b=buffer.get(i);
assert(comparator.compare(a, b)<=0) : "\n"+a+"\n"+b;
// assert(a.mate==null) : "\n"+a+"\n"+b;
}
final Read peek=(q.isEmpty() ? null : q.peek().peek());
final int maxIndex=(peek==null ? buffer.size() : indexOfLowestAbovePivot(buffer, peek));
ArrayList<Read> list=new ArrayList<Read>(maxIndex);
for(int index=0; index<maxIndex; index++){
Read r=buffer.get(index);
assert(peek==null || comparator.compare(peek, r)>0) : "\n"+peek+"\n"+r;
list.add(r);
maxLen=Tools.max(maxLen, r.length());
}
if(ros!=null){ros.add(list, 0);}
ArrayList<Read> oldbuffer=buffer;
buffer=new ArrayList<Read>(2*limit);
for(int i=maxIndex, size=oldbuffer.size(); i<size; i++){
buffer.add(oldbuffer.get(i));
}
if(verbose2){outstream.println("Buffer contains "+buffer.size()+" reads.");}
}
assert(buffer.isEmpty());
synchronized(SortByName.class){
maxLengthObservedStatic=Tools.max(maxLengthObservedStatic, maxLen);
}
}
//
// final Read peek=(q.isEmpty() ? null : q.peek().peek());
//
// final int maxIndex=(peek==null ? buffer.size() : indexOfLowestAbovePivot(buffer, peek));
//
// for(int index=0; index<maxIndex; index++){
// Read r=buffer.get(index);
// list.add(r);
// if(list.size()>=200){
// if(ros!=null){ros.add(list, 0);}
// list=new ArrayList<Read>(200);
// }
// }
//
// if(!list.isEmpty()){
// if(ros!=null){ros.add(list, 0);}
// list=null;
// }
//// if(verbose2){outstream.println("Stopped at index "+index+".");}
//
// ArrayList<Read> oldbuffer=buffer;
// buffer=new ArrayList<Read>(2*limit);
// for(int i=maxIndex, size=oldbuffer.size(); i<size; i++){
// buffer.add(oldbuffer.get(i));
// }
// if(verbose2){outstream.println("Buffer contains "+buffer.size()+" reads.");}
//}
private static final int indexOfLowestAbovePivot(final ArrayList<Read> list, final Read pivot){
final int size=list.size();
final int maxIndex=binarySearch(list, pivot);
if(maxIndex<0){return 0;}
if(maxIndex>=size){return size;}
Read r=list.get(maxIndex);
final int x=comparator.compare(pivot, r);
assert(x<=0) : x+"\n"+pivot.id+"\n"+r.id;
final int ret=(x==0 ? maxIndex+1 : maxIndex);
assert(ret==size || comparator.compare(pivot, list.get(ret))<0);
assert(ret==0 || comparator.compare(pivot, list.get(ret-1))>=0);
return ret;
}
/** Returns highest index of an equal value, or the insert position */
private static final int binarySearch(final ArrayList<Read> list, final Read pivot){
int a=0, b=list.size()-1;
while(b>a){
final int mid=(a+b)/2;
final Read r=list.get(mid);
final int x=comparator.compare(pivot, r);
if(x<0){b=mid;}
else if(x>0){a=mid+1;}
else{return mid;}
}
// for(int i=1; i<list.size(); i++){
// assert(comparator.compare(list.get(i-1), list.get(i))<=0) : i+"\n"+list.get(i-1)+"\n"+ list.get(i)+"\n";
// }
//
// assert(a>=list.size() || comparator.compare(pivot, list.get(a))<=0) :
// comparator.compare(pivot, list.get(a))+"\n"+
// a+", "+b+", "+list.size()+"\n"+pivot.id+"\n"+list.get(a).id;
// comparator.compare(pivot, list.get(a))+", "+a+", "+b+", "+list.size()+"\n"+pivot.id+"\n"+list.get(a-1).id+"\n"+list.get(a).id+"\n"+list.get(a+1).id;
while(a>=0 && a<list.size()-1){
final int x=comparator.compare(pivot, list.get(a+1));
assert(x<=0) : x+", "+a+", "+b+", "+list.size()+"\n"+pivot.id+"\n"+list.get(a).id;
if(x<0){break;}
a++;
}
if(a>=0 && a==list.size()-1){//Corner case; insert after last element was not handled
if(comparator.compare(pivot, list.get(a))>0){a++;}
}
assert(a>=list.size() || comparator.compare(pivot, list.get(a))<=0) :
comparator.compare(pivot, list.get(a))+"\n"+
a+", "+b+", "+list.size()+"\n"+pivot.id+"\n"+list.get(a).id;
return a;
}
private void sortAndDump(final ArrayList<Read> storage, final long currentMem, final AtomicLong outstandingMem, String fname1, String fname2, boolean useHeader) {
String temp1=fname1;
String temp2=fname2;
if(temp1==null){
synchronized(outTemp){
if(verbose2){outstream.println("Synced to outTemp to make a temp file.");}
temp1=getTempFile();
if(verbose2){outstream.println("Temp file: "+temp1);}
outTemp.add(temp1);
}
}
if(verbose || true){outstream.println("Created a WriteThread for "+temp1+(temp2==null ? "" : ", "+temp2));}
WriteThread wt=new WriteThread(storage, currentMem, outstandingMem, temp1, temp2, useHeader, outstream);
wt.start();
}
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
private static class WriteThread extends Thread{
public WriteThread(final ArrayList<Read> storage_, final long currentMem_, final AtomicLong outstandingMem_,
String fname1_, String fname2_, boolean useHeader_, PrintStream outstream_){
storage=storage_;
currentMem=currentMem_;
outstandingMem=outstandingMem_;
fname1=fname1_;
fname2=fname2_;
useHeader=useHeader_;
outstream=outstream_;
}
@Override
public void run(){
if(verbose){outstream.println("Started a WriteThread.");}
final FileFormat ffout1=FileFormat.testOutput(fname1, FileFormat.FASTQ, null, true, false, false, false);
final FileFormat ffout2=FileFormat.testOutput(fname2, FileFormat.FASTQ, null, true, false, false, false);
final ConcurrentReadOutputStream ros;
if(ffout1!=null){
final int buff=4;
ros=ConcurrentReadOutputStream.getStream(ffout1, ffout2, null, null, buff, null, useHeader);
ros.start(); //Start the stream
}else{ros=null;}
if(verbose){outstream.println("Started a ros.");}
Shared.sort(storage, comparator);
if(verbose){outstream.println("Sorted reads.");}
ArrayList<Read> buffer=new ArrayList<Read>(200);
long id=0;
for(int i=0, lim=storage.size(); i<lim; i++){
Read r=storage.set(i, null);
buffer.add(r);
if(buffer.size()>=200){
if(ros!=null){ros.add(buffer, id);}
id++;
buffer=new ArrayList<Read>(200);
}
}
if(ros!=null && buffer.size()>0){ros.add(buffer, id);}
errorState|=ReadWrite.closeStream(ros);
if(verbose){outstream.println("Closed ros.");}
synchronized(outstandingMem){
outstandingMem.addAndGet(-currentMem);
if(verbose){outstream.println("Decremented outstandingMem: "+outstandingMem);}
outstandingMem.notify();
if(verbose){outstream.println("Notified outstandingMem.");}
}
}
final ArrayList<Read> storage;
final long currentMem;
final AtomicLong outstandingMem;
final String fname1;
final String fname2;
final boolean useHeader;
boolean errorState=false;
final PrintStream outstream;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file path */
private String in1=null;
/** Secondary input file path */
private String in2=null;
private String qfin1=null;
private String qfin2=null;
/** Primary output file path */
private String out1=null;
/** Secondary output file path */
private String out2=null;
private ArrayList<String> outTemp=new ArrayList<String>();
/** Override input file extension */
private String extin=null;
/** Override output file extension */
private String extout=null;
private String tempExt=null;
private String giTableFile=null;;
private String taxTreeFile=null;
private String accessionFile=null;
/*--------------------------------------------------------------*/
long maxLengthObserved=0;
private static long maxLengthObservedStatic=0;
private final boolean qualityObserved;
private static boolean qualityObservedStatic;
/** Number of reads processed */
protected long readsProcessed=0;
/** Number of bases processed */
protected long basesProcessed=0;
/** Quit after processing this many input reads; -1 means no limit */
private long maxReads=-1;
private boolean delete=true;
private boolean deleteEarly=false;
private boolean useSharedHeader=false;
private boolean allowTempFiles=true;
private int minlen=0;
private float memBlockMult=0.30f; //Crashed once at old default 0.35
private float memTotalMult=0.65f; //Crashed once at old default 0.75
/** Max files to merge per pass */
private int maxFiles=12;
private boolean setMaxFiles=false;
private static int mergeFileLimit=24;
private boolean genKmer=true;
/*--------------------------------------------------------------*/
/*---------------- Final Fields ----------------*/
/*--------------------------------------------------------------*/
/** Primary input file */
private final FileFormat ffin1;
/** Secondary input file */
private final FileFormat ffin2;
/** Primary output file */
private final FileFormat ffout1;
/** Secondary output file */
private final FileFormat ffout2;
private final boolean clump;
private static ReadComparator comparator=ReadComparatorName.comparator;
/*--------------------------------------------------------------*/
/*---------------- Common Fields ----------------*/
/*--------------------------------------------------------------*/
/** Print status messages to this output stream */
private PrintStream outstream=System.err;
/** Print verbose messages */
public static boolean verbose=false;
/** Print verbose messages */
public static final boolean verbose2=false;
/** True if an error was encountered */
public boolean errorState=false;
/** Overwrite existing output files */
private boolean overwrite=true;
/** Append to existing output files */
private boolean append=false;
}
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