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package stream;
import java.util.ArrayList;
import java.util.Set;
import java.util.concurrent.ArrayBlockingQueue;
import fileIO.FileFormat;
import shared.KillSwitch;
import shared.Parse;
import shared.Shared;
import shared.Tools;
import structures.ByteBuilder;
/**
* Allows output of reads to multiple different output streams.
* Each output stream is controlled by a buffer,
* which stores reads until there is a sufficient quantity to dump.
*
* @author Brian Bushnell
* @date May 14, 2019
*
*/
public abstract class BufferedMultiCros extends Thread {
/*--------------------------------------------------------------*/
/*---------------- Constructors ----------------*/
/*--------------------------------------------------------------*/
public static BufferedMultiCros make(String out1, String out2, boolean overwrite, boolean append,
boolean allowSubprocess, boolean useSharedHeader, int defaultFormat) {
return make(out1, out2, overwrite, append, allowSubprocess, useSharedHeader,
defaultFormat, defaultThreaded, defaultMcrosType, defaultMaxStreams);
}
public static BufferedMultiCros make(String out1, String out2, boolean overwrite, boolean append,
boolean allowSubprocess, boolean useSharedHeader, int defaultFormat, boolean threaded,
int mcrosType, int maxStreams) {
BufferedMultiCros mcros=null;
if(mcrosType==2){//Slow, synchronous mcros type
mcros=new MultiCros2(out1, out2, overwrite, append, true, useSharedHeader, FileFormat.FASTQ, threaded);
}else if(mcrosType==3){//Faster, asynchronous type
mcros=new MultiCros3(out1, out2, overwrite, append, true, useSharedHeader, FileFormat.FASTQ, threaded, maxStreams);
}else if(mcrosType==4){//Threaded file closing
mcros=new MultiCros4(out1, out2, overwrite, append, true, useSharedHeader, FileFormat.FASTQ, threaded, maxStreams);
}else if(mcrosType==5){//New retirement ordering by timer
mcros=new MultiCros5(out1, out2, overwrite, append, true, useSharedHeader, FileFormat.FASTQ, threaded, maxStreams);
}else if(mcrosType==6){//New retirement ordering by timer
mcros=new MultiCros6(out1, out2, overwrite, append, true, useSharedHeader, FileFormat.FASTQ, threaded, maxStreams);
}else{
throw new RuntimeException("Bad mcrosType: "+mcrosType);
}
return mcros;
}
/**
* Primary constructor.
* @param pattern1_ Name pattern for file 1; must contain % (required)
* @param pattern2_ Name pattern for file 2; must contain % (optional)
* @param overwrite_ Permission to overwrite
* @param append_ Permission to append to existing files (this should generally be false)
* @param allowSubprocess_ Allow subprocesses such as pigz, bgzip, or samtools
* @param useSharedHeader_ Print the stored header (from an input sam file) in all output sam files
* @param defaultFormat_ Assume files are in this format if they don't have a valid extension
* @param threaded_ Run this mcros in its own thread
* @param maxStreams_ Max allowed number of concurrent open streams
*/
public BufferedMultiCros(String pattern1_, String pattern2_,
boolean overwrite_, boolean append_, boolean allowSubprocess_, boolean useSharedHeader_,
int defaultFormat_, boolean threaded_, int maxStreams_){
assert(pattern1_!=null && pattern1_.indexOf('%')>=0);
assert(pattern2_==null || pattern1_.indexOf('%')>=0);
//Perform # expansion for twin files
if(pattern2_==null && pattern1_.indexOf('#')>=0){
pattern1=pattern1_.replaceFirst("#", "1");
pattern2=pattern1_.replaceFirst("#", "2");
}else{
pattern1=pattern1_;
pattern2=pattern2_;
}
overwrite=overwrite_;
append=append_;
allowSubprocess=allowSubprocess_;
useSharedHeader=useSharedHeader_;
defaultFormat=defaultFormat_;
threaded=threaded_;
transferQueue=threaded ? new ArrayBlockingQueue<ArrayList<Read>>(8) : null;
maxStreams=maxStreams_;
//Significantly impacts performance.
//Higher numbers give more retires but less time per retire.
//Optimal seems to be around 4-6, at least for 16 streams.
streamsToRetire=Tools.mid(2, (maxStreams+1)/3, 16);
final long bytes=Shared.memAvailable();
memLimitLower=Tools.max(50000000, (long)(memLimitLowerMult*bytes));
memLimitMid=Tools.max(70000000, (long)(memLimitMidMult*bytes));
memLimitUpper=Tools.max(90000000, (long)(memLimitUpperMult*bytes));
}
/*--------------------------------------------------------------*/
/*---------------- Parsing ----------------*/
/*--------------------------------------------------------------*/
public static boolean parseStatic(String arg, String a, String b){
if(a.equals("mcrostype")){
defaultMcrosType=Integer.parseInt(b);
}else if(a.equals("threaded")){
defaultThreaded=Parse.parseBoolean(b);
}else if(a.equals("streams")){
defaultMaxStreams=Integer.parseInt(b);
}else if(a.equalsIgnoreCase("readsPerBuffer")){
defaultReadsPerBuffer=Integer.parseInt(b);
}else if(a.equalsIgnoreCase("bytesPerBuffer")){
defaultBytesPerBuffer=Integer.parseInt(b);
}else if(a.equalsIgnoreCase("memLimitLowerMult") || a.equals("mllmult") || a.equals("mllm")){
memLimitLowerMult=Float.parseFloat(b);
assert(memLimitLowerMult>=0 && memLimitLowerMult<1);
}else if(a.equalsIgnoreCase("memLimitMidMult") || a.equals("mlmmult") || a.equals("mlmm")){
memLimitMidMult=Float.parseFloat(b);
assert(memLimitMidMult>=0 && memLimitMidMult<1);
}else if(a.equalsIgnoreCase("memLimitUpperMult") || a.equals("mlumult") || a.equals("mlum")){
memLimitUpperMult=Float.parseFloat(b);
assert(memLimitUpperMult>=0 && memLimitUpperMult<1);
}else{
return false;
}
return true;
}
/*--------------------------------------------------------------*/
/*---------------- Abstract Methods ----------------*/
/*--------------------------------------------------------------*/
/** True if no errors were encountered */
public abstract boolean finishedSuccessfully();
/**
* Add a single read. Should not be used in threaded mode.
* Should only be used by this class.
* @param r Read to add.
* @param name Name of destination buffer.
*/
abstract void add(Read r, String name);
/**
* Dump all buffered reads to disk, except when minReadsToDump forbids it.
* @return Number of reads dumped.
*/
abstract long dumpAll();
/**
* Dump all residual reads to this stream.
* @param rosu Destination stream.
* @return Number of residual reads dumped.
*/
public abstract long dumpResidual(ConcurrentReadOutputStream rosu);
/** Dump everything and close any open streams. */
abstract long closeInner();
/** Generate a report on how many reads went to each file */
public abstract ByteBuilder report();
/** Time for shutting down output threads */
public String printRetireTime() {
throw new RuntimeException("printRetireTime not available for "+getClass().getName());
}
/** Time for shutting down output threads */
public String printCreateTime() {
throw new RuntimeException("printRetireTime not available for "+getClass().getName());
}
public abstract Set<String> getKeys();
/*--------------------------------------------------------------*/
/*---------------- Final Methods ----------------*/
/*--------------------------------------------------------------*/
/** Shut this down and perform any cleanup needed. */
public final void close(){
if(threaded){poisonAndWait();}
else{closeInner();}
}
/** Primary file pattern */
public final String fname(){return pattern1;}
/** Return true if this stream has detected an error */
public final boolean errorState(){
return errorState;
}
/**
* Send a list of reads to an output buffer.
* The reads must have a name attached to the object field in order to be written.
*/
public final void add(ArrayList<Read> list) {
if(threaded){//Send to the transfer queue
addToQueue(list);
}else{//Add the reads from this thread
addToBuffers(list);
}
}
/** Send individual reads to their designated buffer */
private final void addToBuffers(ArrayList<Read> list) {
for(Read r : list){
if(r.obj!=null){
String name=(String)r.obj;
readsInTotal++;
add(r, name);//Reads without a name in the obj field get ignored here.
}
}
handleLoad0();
}
/** Called to handle load after adding a list */
void handleLoad0() {
//Do nothing
}
/*--------------------------------------------------------------*/
/*---------------- Threaded Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
/** For threaded mode */
public final void run(){
assert(threaded) : "This should only be called in threaded mode.";
try {
for(ArrayList<Read> list=transferQueue.take(); list!=poisonToken; list=transferQueue.take()){
if(verbose){System.err.println("Got list; size=\"+transferQueue.size())");}
addToBuffers(list);
if(verbose){System.err.println("Added list; size="+transferQueue.size());}
}
} catch (InterruptedException e) {
//Terminate JVM if something goes wrong
KillSwitch.exceptionKill(e);
}
closeInner();
}
/** Indicate that no more reads will be sent, for threaded mode */
public final void poison(){
assert(threaded) : "This should only be called in threaded mode.";
addToQueue(poisonToken);
}
boolean addToQueue(ArrayList<Read> list) {
boolean success=false;
for(int i=0; i<10 && !success; i++) {
try {
transferQueue.put(list);
success=true;
} catch (InterruptedException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
if(!success) {
KillSwitch.kill("Something went wrong when adding to "+getClass().getName());
}
return success;
}
/** Indicate that no more reads will be sent, for threaded mode */
public final void poisonAndWait(){
assert(threaded) : "This should only be called in threaded mode.";
poison();
waitForFinish();
}
/** Wait for this object's thread to terminate */
public final void waitForFinish(){
assert(threaded);
if(verbose){System.err.println("Waiting for finish.");}
while(this.getState()!=Thread.State.TERMINATED){
if(verbose){System.err.println("Attempting join.");}
try {
this.join(1000);
} catch (InterruptedException e) {
e.printStackTrace();
}
}
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Output file patterns containing a % symbol */
public final String pattern1, pattern2;
/** True if an error was encountered */
boolean errorState=false;
/** File overwrite permission */
final boolean overwrite;
/** File append permission */
final boolean append;
/** Subprocess spawning permission (e.g., for pigz) */
final boolean allowSubprocess;
/** Output file format, if unclear from file extension */
final int defaultFormat;
/** Buffers for each ReadStreamWriter */
int rswBuffers=1;
/** Print the shared header (for sam files) */
final boolean useSharedHeader;
/** Don't retire below this limit */
final long memLimitLower;
/** Possibly take some action */
final long memLimitMid;
/** Dump everything if this limit is reached from buffered reads */
final long memLimitUpper;
/** Allow this many active streams, for MCros3+ */
public final int maxStreams;
/** Retire this many streams at a time */
public final int streamsToRetire;
/** Dump a buffer once it holds this many reads */
public int readsPerBuffer=defaultReadsPerBuffer;
/** Dump a buffer once it holds this many bytes (estimated) */
public int bytesPerBuffer=defaultBytesPerBuffer;
/** Never write files with fewer than this many reads */
public long minReadsToDump=0;
/** Number of reads encountered that were not written */
public long residualReads=0, residualBases=0;
long readsInTotal=0;
/** Current number of buffered reads */
long readsInFlight=0;
/** Current number of buffered bytes (estimated) */
long bytesInFlight=0;
/** Used when MultiCros is run in threaded mode */
private final ArrayBlockingQueue<ArrayList<Read>> transferQueue;
/** Signal to terminate when in threaded mode */
private final ArrayList<Read> poisonToken=new ArrayList<Read>(0);
/** True if this object is intended to run in a separate thread */
public final boolean threaded;
/** Use a LogLog to track cardinality for each output file */
public boolean trackCardinality=false;
/*--------------------------------------------------------------*/
/*---------------- Static Fields ----------------*/
/*--------------------------------------------------------------*/
private static float memLimitLowerMult=0.20f;
private static float memLimitMidMult=0.40f;
private static float memLimitUpperMult=0.60f;
public static boolean defaultThreaded=true;
public static int defaultMaxStreams=12;
public static int defaultMcrosType=6;
public static int defaultReadsPerBuffer=32000;
public static int defaultBytesPerBuffer=16000000;
public static boolean verbose=false;
}
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