1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579
|
package stream;
import java.io.InputStream;
import java.util.ArrayList;
import java.util.Arrays;
import dna.Gene;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.KillSwitch;
import shared.Shared;
import shared.Timer;
import shared.Tools;
/**
* @author Brian Bushnell
* @date Feb 13, 2013
*
*/
public class FastaReadInputStream extends ReadInputStream {
public static void main(String[] args){
int a=20, b=Integer.MAX_VALUE;
if(args.length>1){a=Integer.parseInt(args[1]);}
if(args.length>2){b=Integer.parseInt(args[2]);}
if(args.length>3){MIN_READ_LEN=Integer.parseInt(args[3]);}
if(args.length>4){TARGET_READ_LEN=Integer.parseInt(args[4]);}
if(TARGET_READ_LEN<1){
TARGET_READ_LEN=Integer.MAX_VALUE;
SPLIT_READS=false;
}else{
SPLIT_READS=true;
}
Timer t=new Timer();
FastaReadInputStream fris=new FastaReadInputStream(args[0], false, false, false, Shared.bufferData());
Read r=fris.next();
int i=0;
while(r!=null){
if(i<a){System.out.println("'"+r.toText(false)+"'");}
r=fris.next();
if(++i>=a){break;}
}
while(r!=null && i++<b){r=fris.next();}
t.stop();
System.out.println("Time: \t"+t);
}
public FastaReadInputStream(String fname, boolean interleaved_, boolean amino_, boolean allowSubprocess_, long maxdata){
this(FileFormat.testInput(fname, FileFormat.FASTA, FileFormat.FASTA, 0, allowSubprocess_, false, false), interleaved_, amino_, maxdata);
}
public FastaReadInputStream(FileFormat ff, boolean interleaved_, boolean amino_, long maxdata){
name=ff.name();
amino=amino_;
flag=(amino ? Read.AAMASK : 0);
if(!fileIO.FileFormat.hasFastaExtension(name) && !name.startsWith("stdin")){
System.err.println("Warning: Did not find expected fasta file extension for filename "+name);
}
interleaved=interleaved_;
allowSubprocess=ff.allowSubprocess();
minLen=MIN_READ_LEN;
maxLen=(SPLIT_READS ? (TARGET_READ_LEN>0 ? TARGET_READ_LEN : Integer.MAX_VALUE) : Integer.MAX_VALUE);
MAX_DATA=maxdata>0 ? maxdata : Shared.bufferData();
ins=open();
assert(settingsOK());
}
@Override
public Read next() {
if(!hasMore()){
if(verbose){System.err.println("hasMore() returned false; currentList="+
(currentList==null ? null : currentList.size())+", nextReadIndex="+nextReadIndex+", consumed="+consumed);}
return null;
}
Read r=currentList.set(nextReadIndex, null);
nextReadIndex++;
consumed++;
return r;
}
@Override
public ArrayList<Read> nextList() {
if(nextReadIndex!=0){throw new RuntimeException("'next' should not be used when doing blockwise access.");}
if(currentList==null || nextReadIndex>=currentList.size()){
boolean b=fillList();
}
ArrayList<Read> list=currentList;
currentList=null;
if(list==null || list.isEmpty()){
list=null;
}else{
consumed+=list.size();
}
return list;
}
@Override
public boolean hasMore() {
if(currentList==null || nextReadIndex>=currentList.size()){
if(open){
fillList();
}else{
// assert(generated>0) : "Was the file empty?";
}
}
return (currentList!=null && nextReadIndex<currentList.size());
}
@Override
public void restart() {
if(ins!=null){close();}
assert(ins==null);
// generated=0;
consumed=0;
nextReadIndex=0;
nextReadID=0;
currentList=null;
header=null;
bstart=0;
bstop=0;
currentSection=0;
if(ins==null){
ins=open();
}else{
assert(false) : "is should be null";
}
}
@Override
public final boolean close(){
synchronized(this){
if(!open){return false;}
open=false;
assert(ins!=null);
try {
if(ins!=System.in){
errorState|=ReadWrite.finishReading(ins, name, allowSubprocess);
}
} catch (Exception e) {
System.err.println("Some error occured: "+e);
errorState=true;
}
ins=null;
}
return false;
}
@Override
public boolean paired() {return interleaved;}
@Override
public void start() {}
private final boolean fillList(){
// assert(open);
if(!open){
currentList=null;
return false;
}
assert(currentList==null || nextReadIndex>=currentList.size());
nextReadIndex=0;
currentList=new ArrayList<Read>(BUF_LEN);
if(header==null){
header=nextHeader();
if(header==null){
currentList=null;
return false;
}
}
long len=0;
for(int i=0; i<BUF_LEN && len<MAX_DATA; i++){
Read r=generateRead(0);
if(r==null){break;}
currentList.add(r);
len+=r.length();
if(interleaved){
Read r2=generateRead(1);
if(r2==null){break;}
len+=r2.length();
r.mate=r2;
r2.mate=r;
}
nextReadID++;
if(verbose){System.err.println("Generated a read; i="+i+", BUF_LEN="+BUF_LEN);}
// if(i==1){assert(false) : r.numericID+", "+r.mate.numericID;}
}
return currentList.size()>0;
}
private final Read generateRead(int pairnum){
if(verbose){System.err.println("Called generateRead(); bstart="+bstart+", bstop="+bstop+", currentSection="+currentSection+", header="+header);}
assert(header!=null) : "Null header for fasta read - input file may be corrupt: "+name;
if(bstart<bstop && buffer[bstart]==carrot){
header=nextHeader();
currentSection=0;
}
if(header==null){
close();
return null;
}
byte[] bases=nextBases();
currentSection++;
while(bases==null){
// if(!open){return null;} //Should not be needed...
header=nextHeader();
if(header==null){
close();
return null;
}
bases=nextBases();
currentSection=1;
}
assert(bases!=null);
assert(bases.length>0);
byte[] quals=null;
if(FAKE_QUALITY){
quals=new byte[bases.length];
Arrays.fill(quals, (byte)(Shared.FAKE_QUAL));
}
// String hd=((currentSection==1 && !hitmax) ? header : header+"_"+currentSection);
String hd=((!FORCE_SECTION_NAME && currentSection==1 && bases.length<=maxLen) ? header : header+"_part_"+currentSection);
// assert(false) : FORCE_SECTION_NAME+", "+(currentSection==1)+", "+(bases.length<=maxLen)+", "+bases.length+", "+maxLen;
assert(currentSection==1 || bases.length>0) : "id="+hd+", section="+currentSection+", len="+bases.length+"\n"+new String(bases);
Read r=null;
if(FASTQ.PARSE_CUSTOM){//TODO: This appears to be legacy code from old custom header format, which won't work anymore
assert(false) : "TODO - this code for parsing custom headers of fasta files is obsolete.";
if(header!=null && header.indexOf('_')>0){
String temp=header;
if(temp.endsWith(" /1") || temp.endsWith(" /2")){temp=temp.substring(0, temp.length()-3);}
String[] answer=temp.split("_");
if(answer.length>=5){
try {
int trueChrom=Gene.toChromosome(answer[1]);
byte trueStrand=Byte.parseByte(answer[2]);
int trueLoc=Integer.parseInt(answer[3]);
int trueStop=Integer.parseInt(answer[4]);
// r=new Read(bases, trueChrom, trueStrand, trueLoc, trueStop, hd, quals, nextReadID);
r=new Read(bases, quals, hd, nextReadID, (flag|trueStrand), trueChrom, trueLoc, trueStop);
r.setSynthetic(true);
} catch (NumberFormatException e) {
FASTQ.PARSE_CUSTOM=false;
System.err.println("Turned off PARSE_CUSTOM because could not parse "+new String(header));
}
}else{
FASTQ.PARSE_CUSTOM=false;
System.err.println("Turned off PARSE_CUSTOM because answer="+Arrays.toString(answer));
}
}else{
FASTQ.PARSE_CUSTOM=false;
System.err.println("Turned off PARSE_CUSTOM because header="+header+", index="+header.indexOf('_'));
}
}
if(r==null){
r=new Read(bases, quals, hd, nextReadID, flag);
}
r.setPairnum(pairnum);
if(verbose){System.err.println("Made read:\t"+(r.length()>1000 ? r.id : r.toString()));}
return r;
}
private String nextHeader(){
if(verbose){System.err.println("Called nextHeader(); bstart="+bstart+"; bstop="+bstop);}
assert(bstart>=bstop || buffer[bstart]=='>' || buffer[bstart]<=slashr) : bstart+", "+bstop+", '"+(char)buffer[bstart]+"'"+"\t"+name;
while(bstart<bstop && buffer[bstart]!='>'){bstart++;}
int x=bstart;
assert(bstart>=bstop || buffer[x]=='>') : bstart+", "+bstop+", '"+(char)buffer[x]+"'";
while(x<bstop && buffer[x]>slashr){x++;}
if(verbose){System.err.println("A: x="+x);}
if(x<bstop && (buffer[x]<=STX || buffer[x]==tab)){ //Handle deprecated 'SOH' symbol and tab
while(x<bstop && (buffer[x]>slashr || buffer[x]<=STX || buffer[x]==tab)){
if(buffer[x]==0x1){buffer[x]=carrot;}
x++;
}
}
if(verbose){System.err.println("B: x="+x);}
if(x>=bstop){
int fb=fillBuffer();
if(verbose){System.err.println("B: fb="+fb);}
if(fb<1){
if(verbose){System.err.println("Returning null from nextHeader()");}
return null;
}
x=0;
assert(bstart==0 && bstart<bstop && buffer[x]=='>') : "Improperly formatted fasta file; expecting '>' symbol.\n"+
(buffer[x]=='@' ? "If this is a fastq file, please rename it with a '.fastq' extension.\n" : "")+
bstart+", "+bstop+", "+(int)buffer[x]+", "+(char)buffer[x]; //Note: This assertion will fire if a fasta file starts with a newline.
while(x<bstop && buffer[x]>slashr){x++;}
if(x<bstop && (buffer[x]<=STX || buffer[x]==tab)){ //Handle deprecated 'SOH' symbol and tab
while(x<bstop && (buffer[x]>slashr || buffer[x]<=STX || buffer[x]==tab)){
if(buffer[x]==0x1){buffer[x]=carrot;}
x++;
}
}
}
if(verbose){System.err.println("C: x="+x);}
assert(x>=bstop || buffer[x]<=slashr);
int start=bstart+1, stop=x;
if(Shared.TRIM_READ_COMMENTS){
for(int i=start; i<stop; i++){
if(Character.isWhitespace(buffer[i])){
stop=i;
break;
}
}
}
String s=stop>start ? new String(buffer, start, stop-start) : "";
// String s=new String(buffer, bstart+1, x-(bstart+1));
if(verbose){System.err.println("Fetched header: '"+s+"'");}
bstart=x+1;
return s;
}
private byte[] nextBases(){
if(verbose){System.err.println("Called nextBases()");}
// assert(open) : "Attempting to read from a closed file. Current header: "+header;
if(bstart>=bstop){
int bytes=fillBuffer();
if(bytes<1 || !open){return null;}
}
int x=bstart;
int bases=0;
if(!(x>=bstop || buffer[x]!='>')){
handleNoSequence(x);
}
// assert(x>=bstop || buffer[x]!='>') :
// "A fasta header with no sequence was encountered. To discard such headers, please re-run with the -da flag.";
//"\n<START>"+new String(buffer, 0, Tools.min(x+1, buffer.length))+"<STOP>\n";
while(x<bstop && bases<maxLen && buffer[x]!='>'){
while(x<bstop && bases<maxLen && buffer[x]!='>'){
if(buffer[x]>slashr){bases++;}
x++;
}
assert(x==bstop || buffer[x]=='>' || bases==maxLen);
if(x==bstop && bases<maxLen){
int fb=fillBuffer();
if(fb<1){
x=bstop;
if(verbose){System.err.println("Broke loop when fb="+fb+"; bstart="+bstart+", bstop="+bstop);}
break;
}
x=bstart;
bases=0;
}
}
if(bases<minLen){
if(bases==0){handleNoSequence(x);}
// assert(open) : "Attempting to read from a closed file. Current header: "+header;
bstart=x;
if(verbose){System.err.println("Fetched "+bases+" bases; returning null. bstart="+bstart+", bstop="+bstop/*+"\n"+new String(buffer)*/);}
return null;
}
byte[] r=new byte[bases];
// if(Read.TO_UPPER_CASE){
// for(int i=bstart, j=0; j<bases; i++){
// assert(i<x);
// byte b=buffer[i];
// // if(verbose){System.err.println("grabbed base "+(char)b+" = "+b);}
// if(b>slashr){
// r[j]=(b<91 ? b : (byte)(b-32));//Convert to upper case
// // if(verbose){System.err.println("set to base "+(char)r[j]+" = "+r[j]);}
// j++;
// }
// }
// }else{
for(int i=bstart, j=0; j<bases; i++){
assert(i<x);
byte b=buffer[i];
if(b>slashr){
r[j]=b;
j++;
}
}
// }
if(verbose){System.err.println("Fetched "+bases+" bases, open="+open+":\n'"+(r.length>1000 ? "*LONG*" : new String(r))+"'");}
bstart=x;
return r;
}
private void handleNoSequence(int x){
if(currentSection>0){return;}//This section is spuriously entered for reads that are a multiple of the target read length when splitting.
if(WARN_IF_NO_SEQUENCE){
synchronized(getClass()){
if(reportedHeader==header){return;}
reportedHeader=header;
System.err.println("Warning: A fasta header with no sequence was encountered ("+currentSection+"):\n"+header+"\n");
if(WARN_FIRST_TIME_ONLY){WARN_IF_NO_SEQUENCE=false;}
}
}
assert(!ABORT_IF_NO_SEQUENCE) : "\n<START>"+new String(buffer, 0, Tools.min(x+1, buffer.length))+"<STOP>\n";
}
/** Fills buffer. Ensures that result will extend to the next caret or EOF. Returns number of bytes filled. */
private final int fillBuffer(){
// assert(open);
if(!open){return 0;}
if(verbose){System.err.println("fillBuffer() : bstart="+bstart+", bstop="+bstop);}
if(bstart<bstop){ //Shift end bytes to beginning
if(bstart>0){
// assert(bstart>0) : bstart+", "+bstop+", "+new String(buffer);
int extra=bstop-bstart;
for(int i=0; i<extra; i++, bstart++){
buffer[i]=buffer[bstart];
}
bstop=extra;
}
}else{
bstop=0;
}
if(verbose){System.err.println("After shift : bstart="+bstart+", bstop="+bstop);}
// assert(bstart>0 || buffer[0]=='>') : "bstart="+bstart+", bstop="+bstop+", buffer[0]='"+(char)buffer[0]+"'";
// assert(bstart<=bstop) : "bstart="+bstart+", bstop="+bstop+", buffer[0]='"+(char)buffer[0]+"'";
bstart=0;
int len=bstop;
int r=-1;
int sum=0;
boolean seenNewline=false;
while(len==bstop){//hit end of input without encountering a caret
if(bstop==buffer.length){
buffer=KillSwitch.copyOf(buffer, buffer.length*2L);
if(verbose){System.err.println("Resized to "+buffer.length);}
}
if(verbose){System.err.println("A: bstop="+bstop+", len="+len);}
try {
r=-1;
r=ins.read(buffer, bstop, buffer.length-bstop);
} catch (Exception e) {
//e.printStackTrace(); //This can happen sometimes when using a fixed number of reads.
}
if(verbose){System.err.println("B: r="+r);}
//if(verbose){System.err.println("r="+r);}
if(r>0){
sum+=r;
bstop=bstop+r;
if(bstop>0 && len==0){len=1;}//Probably to skip the first >
while(len<bstop && (buffer[len]!=carrot || !seenNewline)){//I need to see a caret after newline
seenNewline|=(buffer[len]=='\n');
len++;
}
if(verbose){System.err.println("C: bstop="+bstop+", len="+len+", seenNewline="+seenNewline/*+", seenCarrot="+seenCarrot*/);}
}else{
len=bstop;
if(verbose){System.err.println("D: bstop="+bstop+", len="+len);}
break;
}
}
if(verbose){System.err.println("E: bstop="+bstop+", len="+len+", r="+r);}
//Skip ';'-delimited comments
if(header==null && bstop>bstart && buffer[bstart]==';'){
if(sum==0){return sum;}
int lastsemi=bstart;
assert(nextReadID==0);
assert(bstart==0);
while(bstop>bstart && buffer[bstart]==';'){
while(bstop>bstart && (buffer[bstart]>slashr || buffer[bstart]<=STX || buffer[bstart]==tab)){bstart++;}
while(bstop>bstart && buffer[bstart]<=slashr){bstart++;}
}
if(bstart>=bstop){ //Overflowed buffer with comments; recur
bstart=lastsemi;
return fillBuffer();
}
}
assert(r==-1 || buffer[len]=='>');
if(verbose){System.err.println("After filling: bstart="+bstart+", bstop="+bstop+", len="+len+", r="+r+", sum="+sum);}
return sum;
}
private final InputStream open(){
if(open){
throw new RuntimeException("Attempt to open already-opened fasta file "+name);
}
open=true;
ins=ReadWrite.getInputStream(name, true, allowSubprocess);
bstart=0;
bstop=0;
// lasteol=-1;
return ins;
}
public boolean isOpen(){return open;}
/** Validate fasta settings for auto-shredding input.
* This is kind of obsolete legacy code for BBMap. */
public static final boolean settingsOK(){
if(MIN_READ_LEN>=Integer.MAX_VALUE-1){
throw new RuntimeException("Minimum FASTA read length is too long: "+MIN_READ_LEN);
}
if(MIN_READ_LEN<1){
throw new RuntimeException("Minimum FASTA read length is too short: "+MIN_READ_LEN);
}
if(SPLIT_READS){
if(TARGET_READ_LEN<1){
throw new RuntimeException("Target FASTA read length is too short: "+TARGET_READ_LEN);
}
if(MIN_READ_LEN>TARGET_READ_LEN){
throw new RuntimeException("Minimum FASTA read length is longer than maximum read length: "+MIN_READ_LEN+">"+TARGET_READ_LEN);
}
}
if(MIN_READ_LEN>=Integer.MAX_VALUE-1 || MIN_READ_LEN<1){return false;}
if(SPLIT_READS && (TARGET_READ_LEN<1 || MIN_READ_LEN>TARGET_READ_LEN)){return false;}
return true;
}
@Override
public String fname(){return name;}
public final String name;
private ArrayList<Read> currentList=null;
private String header=null;
private String reportedHeader=null;
private boolean open=false;
private byte[] buffer=new byte[16384];
private int bstart=0, bstop=0;
public InputStream ins;
private long consumed=0;
private long nextReadID=0;
private int nextReadIndex=0;
private int currentSection=0;
public final boolean allowSubprocess;
public final boolean interleaved;
public final boolean amino;
public final int flag;
private final int BUF_LEN=Shared.bufferLen();
private final long MAX_DATA;
private final int maxLen, minLen;
public static boolean verbose=false;
private static final byte slashr='\r', slashn='\n', carrot='>', space=' ', tab='\t', SOH=0x1, STX=0x2;
public static boolean SPLIT_READS=false;
public static int TARGET_READ_LEN=500;
public static int MIN_READ_LEN=1;
public static boolean FAKE_QUALITY=false;
public static boolean FORCE_SECTION_NAME=false;
public static boolean WARN_IF_NO_SEQUENCE=true;
public static boolean WARN_FIRST_TIME_ONLY=true;
public static boolean ABORT_IF_NO_SEQUENCE=false;
}
|