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package stream;
import java.io.File;
import java.util.ArrayList;
import java.util.LinkedHashMap;
import java.util.Map.Entry;
import java.util.Set;
import fileIO.FileFormat;
import fileIO.ReadWrite;
import shared.KillSwitch;
import shared.Shared;
import structures.ByteBuilder;
import structures.ListNum;
/**
* This implementation allows only a single, synchronous open stream.
*
* @author Brian Bushnell
* @date May 1, 2019
*
*/
public class MultiCros2 extends BufferedMultiCros {
/** For testing */
public static void main(String[] args){
String in=args[0];
String pattern=args[1];
ArrayList<String> names=new ArrayList<String>();
for(int i=2; i<args.length; i++){
names.add(args[i]);
}
MultiCros2 mcros=new MultiCros2(pattern, null, false, false, false, false, FileFormat.FASTQ, false);
ConcurrentReadInputStream cris=ConcurrentReadInputStream.getReadInputStream(-1, true, false, in);
cris.start();
ListNum<Read> ln=cris.nextList();
ArrayList<Read> reads=(ln!=null ? ln.list : null);
while(ln!=null && reads!=null && reads.size()>0){//ln!=null prevents a compiler potential null access warning
for(Read r1 : reads){
mcros.add(r1, r1.barcode(true));
}
cris.returnList(ln);
ln=cris.nextList();
reads=(ln!=null ? ln.list : null);
}
cris.returnList(ln);
ReadWrite.closeStreams(cris);
mcros.dumpAll();
mcros.close();
}
/*--------------------------------------------------------------*/
/*---------------- Initialization ----------------*/
/*--------------------------------------------------------------*/
/** @See Details in superclass constructor */
public MultiCros2(String pattern1_, String pattern2_,
boolean overwrite_, boolean append_, boolean allowSubprocess_, boolean useSharedHeader_, int defaultFormat_, boolean threaded_){
super(pattern1_, pattern2_, overwrite_, append_, allowSubprocess_, useSharedHeader_, defaultFormat_, threaded_, 1);
bufferMap=new LinkedHashMap<String, Buffer>();
}
/*--------------------------------------------------------------*/
/*---------------- Outer Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
public boolean finishedSuccessfully(){
return !errorState;
}
@Override
public void add(Read r, String name){
assert(!threaded);
Buffer b=bufferMap.get(name);
if(b==null){
b=new Buffer(name);
bufferMap.put(name, b);
//Note: I could adjust bytesPerBuffer threshold here in response to the number of buffers.
}
b.add(r);
// System.err.println("Added "+name);
}
@Override
public long dumpResidual(ConcurrentReadOutputStream rosu){
long dumped=0;
//For each Buffer, check if it contains residual reads
//If so, dump it into the stream
for(Entry<String, Buffer> e : bufferMap.entrySet()){
Buffer b=e.getValue();
assert((b.readsIn<minReadsToDump) == (b.list!=null && !b.list.isEmpty()));
if(b.readsIn>0 && b.readsIn<minReadsToDump){
assert(b.list!=null && !b.list.isEmpty());
residualReads+=b.readsIn;
residualBases+=b.basesIn;
if(rosu!=null){rosu.add(b.list, 0);}
}
b.list=null;
}
return dumped;
}
@Override
public ByteBuilder report(){
ByteBuilder bb=new ByteBuilder(1024);
if(minReadsToDump>0){
bb.append("Residual").tab().append(residualReads).tab().append(residualBases).nl();
}
for(Entry<String, Buffer> e : bufferMap.entrySet()){
Buffer b=e.getValue();
if(b.readsIn>=minReadsToDump){
bb.append(b.name).tab().append(b.readsIn).tab().append(b.basesIn).nl();
}
}
return bb;
}
@Override
public Set<String> getKeys(){return bufferMap.keySet();}
/*--------------------------------------------------------------*/
/*---------------- Inner Methods ----------------*/
/*--------------------------------------------------------------*/
@Override
long closeInner() {
long x=dumpAll();
return x;
}
@Override
long dumpAll(){
long dumped=0;
for(Entry<String, Buffer> e : bufferMap.entrySet()){
dumped+=e.getValue().dump();
}
return dumped;
}
/*--------------------------------------------------------------*/
/*---------------- Getters ----------------*/
/*--------------------------------------------------------------*/
/*--------------------------------------------------------------*/
/*---------------- Inner Classes ----------------*/
/*--------------------------------------------------------------*/
/**
* A Buffer holds reads destined for to a specific file.
* When sufficient reads are present, it opens a stream and writes them,
* then closes the stream.
*/
private class Buffer {
Buffer(String name_){
name=name_;
String s1=pattern1.replaceFirst("%", name);
String s2=pattern2==null ? null : pattern2.replaceFirst("%", name);
//These are created with overwrite=false append=true because
//the files will be appended to if the stream gets prematurely retired.
//Therefore, files must be explicitly deleted first.
//Alternative would be to create a new FileFormat each time.
ff1=FileFormat.testOutput(s1, defaultFormat, null, allowSubprocess, false, true, false);
ff2=FileFormat.testOutput(s2, defaultFormat, null, allowSubprocess, false, true, false);
list=new ArrayList<Read>(readsPerBuffer);
if(verbose){System.err.println("Made buffer for "+name);}
}
/**
* Add a read to this buffer, and update all the tracking variables.
* This may trigger a dump.
*/
void add(Read r){
list.add(r);
long size=r.countPairBytes();
int count=r.pairCount();
currentBytes+=size;
bytesInFlight+=size;
basesIn+=size;
readsInFlight+=count;
readsIn+=count;
if(list.size()>=readsPerBuffer || currentBytes>=bytesPerBuffer){
if(verbose){
System.err.println("list.size="+list.size()+"/"+readsPerBuffer+
", bytes="+currentBytes+"/"+bytesPerBuffer+", bytesInFlight="+bytesInFlight+"/"+memLimitUpper);
}
dump();
}
//Too much buffered data; dump everything.
if(bytesInFlight>=memLimitUpper){
long dumped=dumpAll();
if(dumped<1 && Shared.EA()){
KillSwitch.kill("\nThis program ran out of memory."
+ "\nTry increasing the -Xmx flag or get rid of the minreads flag,"
+ "\nor disable assertions to skip this message and try anyway.");
}
}
}
/** Create a stream, dump buffered reads, and close the stream */
long dump(){
// System.err.println("Dumping "+name);
if(list.isEmpty() || readsIn<minReadsToDump){return 0;}
if(numDumps==0 && overwrite){
delete(ff1);
delete(ff2);
}
// assert(false) : counter+", "+overwrite;
final long size0=list.size();
ConcurrentReadOutputStream ros=ConcurrentReadOutputStream.getStream(ff1, ff2, rswBuffers, null, useSharedHeader && numDumps==0);
ros.start();
ros.add(list, 0);
errorState=ReadWrite.closeStream(ros) | errorState;
// System.err.println("Closed stream "+name);
bytesInFlight-=currentBytes;
readsInFlight-=size0;
readsWritten+=size0;
currentBytes=0;
numDumps++;
list=new ArrayList<Read>(readsPerBuffer);
return size0;
}
private void delete(FileFormat ff){
if(ff==null){return;}
File f=new File(ff.name());
if(f.exists()){f.delete();}
}
/** Current list of buffered reads */
private ArrayList<Read> list;
/** Stream name, which is the variable part of the file pattern */
private final String name;
/** Output file 1 */
private final FileFormat ff1;
/** Output file 2 */
private final FileFormat ff2;
/** Number of reads entering the buffer */
private long readsIn=0;
/** Number of bases entering the buffer */
private long basesIn=0;
/** Number of reads written to disk */
@SuppressWarnings("unused")
private long readsWritten=0;//This does not count read2!
/** Number of bytes currently in this buffer (estimated) */
private long currentBytes=0;
/** Number of dumps executed */
private long numDumps=0;
}
/*--------------------------------------------------------------*/
/*---------------- Fields ----------------*/
/*--------------------------------------------------------------*/
/** Map of names to buffers */
public final LinkedHashMap<String, Buffer> bufferMap;
}
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